期刊
ACS SYNTHETIC BIOLOGY
卷 6, 期 11, 页码 2014-2020出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.7b00149
关键词
mammalian synthetic biology; gene expression; genetic circuits; Q-system; embryonic stem cells
资金
- University of Utah
- National Science Foundation CAREER Program [CBET-1554017]
- Office of Naval Research [N00014-16-1-3012]
- University of Utah Undergraduate Research Opportunities Program (UROP)
- University of Utah Flow Cytometry Facility
- National Cancer Institute [5P30CA042014-24]
- National Center for Research of the National Institutes of Health [1S10RR026802-01]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1554017] Funding Source: National Science Foundation
The field of mammalian synthetic biology seeks to engineer enabling technologies to create novel approaches for programming cells to probe, perturb, and regulate gene expression with unprecedented precision. To accomplish this, new genetic parts continue to be identified that can be used to build novel genetic circuits to re-engineer cells to perform specific functions. Here, we establish a new transcription-based genetic circuit that combines genes from the quinic acid sensing metabolism of Neorospora crassa and the bacterial Lac repressor system to create a new orthogonal genetic tool to be used in mammalian cells. This work establishes a novel genetic tool, called LacQ, that functions to regulate gene expression in Chinese hamster ovarian (CHO) cells, human embryonic kidney 293 (HEK293) cells, and in mouse embryonic stem (ES) cells.
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