☆ 4.7 Article

CAGE: Chromatin Analogous Gene Expression

ACS SYNTHETIC BIOLOGY (2017)

期刊

ACS SYNTHETIC BIOLOGY

卷 6, 期 10, 页码 1800-1806

出版社

AMER CHEMICAL SOC

DOI: 10.1021/acssynbio.7b00045

关键词

CAGE; molecular machine; gene expression; RNA polymerase regulation

类别

Biochemical Research Methods

资金

Micron Foundation
National Institute of General Medical Sciences of the National Institutes of Health [K25GM093233]
National Science Foundation [CMMI-1344915]
FAME
MARCO
DARPA

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Protocol

Reagent

摘要

Self-assembled nucleic acids perform biological, chemical, and mechanical work at the nanoscale. DNA-based molecular machines have been designed here to perform work by reacting with cancer-specific miRNA mimics and then regulating gene expression in, vitro by tuning RNA polymerase activity. Because RNA production is topologically restrained, the machines demonstrate chromatin analogous gene expression (CAGE). With modular and tunable design features, CAGE has potential for molecular biology, synthetic biology, and personalized medicine applications.

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