Article
Microbiology
Nicolas L. Fernandez, Ziyuan Chen, David E. H. Fuller, Lieke A. van Gijtenbeek, Taylor M. Nye, Julie S. Biteen, Lyle A. Simmons
Summary: DnmA is a DNA methyltransferase that forms N6-methyladenosine in Bacillus subtilis and its activity is regulated by prior methylation and RNA-DNA hybrids. DnmA is localized to the replisome region and the nucleoid, and it is excluded from the nucleoid under conditions of high DNA methylation, RNA-DNA hybrid accumulation, or limited DNA binding activity.
Article
Environmental Sciences
Hongfei Lu, Xuebin Qi, Shafeeq Ur Rahman, Dongmei Qiao, Ping Li, Yang Han, Zhijuan Zhao
Summary: The increasing salinity levels under reclaimed water irrigation have negative effects on plant growth, but the combination of B. subtilis and S. cerevisiae can promote rice physiological indices, with B. subtilis having a greater effect. Soil nitrogen concentration and the amount of B. subtilis applied are major factors influencing leaf physiological indices.
Article
Agricultural Engineering
Hongfei Lu, Xuebin Qi, Ping Li, Dongmei Qiao, Shafeeq-ur-Rahman, Fangfang Bai, Jiaxin Cui
Summary: Reclaimed water-fresh water combined irrigation increased the plant height and yield of rice. Inoculation with Bacillus subtilis and Saccharomyces cerevisiae enhanced nutrient absorption capacity and improved soil fertility and texture, while reducing soil salt accumulation.
INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING
(2022)
Article
Food Science & Technology
Vasilios Tsiouris, Panagiotis Tassis, Jog Raj, Tilemachos Mantzios, Konstantinos Kiskinis, Marko Vasiljevic, Nikola Delic, Evanthia Petridou, Georgia D. Brellou, Zoe Polizopoulou, Nikolaos Mittas, Ioanna Georgopoulou
Summary: The study demonstrated that the supplementation of MMDA significantly improved the adverse effects of AFB(1) and OTA in broilers, including increased feed conversion ratio, reduced biochemical alterations, decreased intestinal pH values, and lower E. coli counts in the ceca of birds.
Review
Biochemical Research Methods
Mengyao Lu, Imann Mosleh, Alireza Abbaspourrad
Summary: Human milk oligosaccharides (HMOs) are important components in human milk, and the engineered microbial routes have been researched for large-scale production. The selection of host strains and expression of appropriate glycosyltransferase are key factors in HMOs production using engineered microbial pathways.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jiheng Tian, Baowen Xing, Mengyuan Li, Changgeng Xu, Yi-Xin Huo, Shuyuan Guo
Summary: Bacillus subtilis is a versatile microbial cell factory that can produce valuable proteins and value-added chemicals. Researchers have developed an efficient CRISPR-Cas9 method for large-scale and scarless genome engineering in Bacillus subtilis. They were able to delete DNA fragments up to 134.3 kb, 3.5 times longer than previous reports, with a positivity rate of 100%. The study also investigated the effects of using a heterologous NHEJ system, linear donor DNA, and various donor DNA lengths on the engineering efficiencies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Patrick Becker, Paul Esker, Gerardina Umana
Summary: The study shows that by rotating the use of biological pesticides and chemical fungicides, the severity of Black sigatoka can be significantly reduced, lowering pathogen resistance to fungicides and decreasing the use of chemical pesticides.
Article
Biochemistry & Molecular Biology
Hong Zhang, Parker Murphy, Jason Yu, Sukyeong Lee, Francis T. F. Tsai, Ambro van Hoof, Jiqiang Ling
Summary: Aminoacyl-tRNA synthetases (aaRSs), essential enzymes for protein synthesis, can cause neurological disorders when mutated. This study investigates the mechanism behind these mutations and found that combined defects in aminoacylation and editing result in severe proteotoxicity. The results show that aminoacylation deficiency predisposes cells to proteotoxic stress and impairs ribosome-associated quality control.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Biochemical Research Methods
Shen-Long Tsai, Qing Sun, Wilfred Chen
Summary: The widespread use of biomass for energy and chemical production is hindered by its recalcitrant nature. Consolidated bioprocessing (CBP) can reduce overall cost by combining enzyme production, biomass hydrolysis, and sugar fermentation. Cellulosomes found in anaerobic microorganisms are efficient for biomass depolymerization. However, the conversion rate of synthetic cellulosomes is currently low. Displaying more complex cellulosome structures and tethering them onto living cells hold the potential to enhance overall efficiency.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Mehdi Pakizeh, Leila Nouri, Mohammad Hossein Azizi
Summary: Probiotics are an effective and safe method to reduce toxins in pistachio paste. By increasing the number of microorganisms and decreasing the toxin level, a higher percentage of toxins can be removed. The study found that the addition of probiotics significantly reduced the level of aflatoxin B1 in pistachio paste to permissible limits.
JOURNAL OF FOOD QUALITY
(2022)
Article
Agriculture, Dairy & Animal Science
Andressa Vieira de Moraes, Marco Shizuo Owatari, Eduardo da Silva, Marina de Oliveira Pereira, Marina Piola, Claudio Ramos, Daniel Rosa Farias, Delano Dias Schleder, Gabriel Fernandez Alves Jesus, Adolfo Jatoba
Summary: This study evaluated the use of two microencapsulated probiotic additives in extruded diets for juvenile Nile tilapia, and found that the addition of probiotics improved the zootechnical and health aspects of the fish. The fish fed with probiotic diets showed greater survival, improvements in feed conversion, as well as in the specific growth rate. Histological and histomorphometric analysis revealed that the probiotic treatments had a positive effect on the intestinal health of the fish. Metagenomic evaluation also showed significant differences in the intestinal microbiomes between the treatments. In the challenge against the pathogen A. hydrophila, the probiotic treatments exhibited lower cumulative mortality compared to the control group.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Chenyun Wang, Wei Zhang, Rongzhen Tian, Jianing Zhang, Linpei Zhang, Zhaohong Deng, Xueqin Lv, Jianghua Li, Long Liu, Guocheng Du, Yanfeng Liu
Summary: In this study, a multi-view learning strategy was used to generate synthetic NCSs for regulating gene expression in S. cerevisiae and B. subtilis. The synthetic NCSs improved bioproduction of specific compounds in these microorganisms.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Akira Nishimura, Ryoya Tanahashi, Tomoki Oi, Kyoyuki Kan, Hiroshi Takagi
Summary: The current CRISPR/Cas9 systems in Saccharomyces cerevisiae cannot be considered non-genetic modification technology due to the need for plasmid expression systems. However, our study demonstrates that editing the yeast genome can be achieved without plasmid expression systems, using a commercially available protein transfection reagent and chemically modified sgRNAs.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yu Zhao, Camila Coelho, Stephanie Lauer, Milosz Majewski, Jon M. Laurent, Ran Brosh, Jef D. Boeke
Summary: The use of synthetic genomics has revolutionized our ability to answer fundamental biological questions by designing and building "big" DNA constructs. Saccharomyces cerevisiae, or budding yeast, is a powerful platform for assembling large synthetic constructs, but introducing designer variations to episomal assemblies remains challenging. The CRISPR Engineering of EPisomes in Yeast (CREEPY) method provides a rapid and efficient way to edit large synthetic episomal DNA constructs.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Engineering, Environmental
Yan Xia, Lichao Sun, Zeyu Liang, Yingjie Guo, Jing Li, Dan Tang, Yi-Xin Huo, Shuyuan Guo
Summary: SpRY overcomes the PAM recognition barrier in Bacillus subtilis and enables the development of a highly efficient PAM-less base editing toolbox. This toolbox allows for multiplexed gene editing and has been successfully applied in gene knockout and translation control. It shows great potential in metabolic engineering and codon expansion.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Yu Zhang, Yun Zhang, Xiuling Shang, Bo Wang, Qitiao Hu, Shuwen Liu, Tingyi Wen
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Article
Biochemistry & Molecular Biology
Hongwei Su, Tao Tao, Zhao Yang, Xing Kang, Xu Zhang, Danyue Kang, Song Wu, Chong Li
Article
Biotechnology & Applied Microbiology
Shuwen Liu, Haihan Xiao, Fangfang Zhang, Zheng Lu, Yun Zhang, Aihua Deng, Zhongcai Li, Cui Yang, Tingyi Wen
BIOTECHNOLOGY FOR BIOFUELS
(2019)
Article
Microbiology
Hua Bai, Huibin Lv, Aihua Deng, Xiaodan Jiang, Xuemin Li, Tingyi Wen
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Faiza Naz, Yixin Wu, Nan Zhang, Zhao Yang, Changyuan Yu
Article
Neurosciences
Jie Wu, Jing-Qiu Qu, Yan-Jun Zhou, Yong-Jian Zhou, Yuan-Yuan Li, Nan-Qu Huang, Cheng-Min Deng, Yong Luo
Article
Cell Biology
Zhao Yang, Haifeng Wang, Nan Zhang, Tianying Xing, Wei Zhang, Guoqing Wang, Chong Li, Changyuan Yu
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Respiratory System
Ling Gong, Gang Liu, Honglan Zhu, Caihong Li, Pengmei Li, Changlu Liu, Hongbo Tang, Kaifeng Wu, Jie Wu, Daishun Liu, Xiaoping Tang
BMC PULMONARY MEDICINE
(2020)
Article
Ophthalmology
Dalan Jing, Aihua Deng, Hongmei Wang, Yilin Chou, Xiaodan Jiang, Zhenxiang Chen, Xuemin Li, Tingyi Wen
Summary: The study compared the safety and satisfaction of bandage contact lens (BCL) and eye patching in patients after cataract surgery. Results showed that the patient satisfaction scores of the BCL group were higher, with patients more likely to choose the same treatment and recommend BCLs to others.
JOURNAL OF OPHTHALMOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Song Qiu, Zhenyu Guo, Faiza Naz, Zhao Yang, Changyuan Yu
Summary: This review discusses the utilization, development, and cost of cathode materials in MFCs, highlighting effective modification methods that have led to improved power generation in recent years.
BIOELECTROCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Yun Zhang, Zhe Liu, Xueliang Wang, Hui Jian, Haihan Xiao, Tingyi Wen
Summary: Mitochondrial serine hydroxymethyltransferase (SHMT2) supports the proliferation of bladder cancer cells by maintaining redox homeostasis and its deficiency triggers cell apoptosis through ROS-dependent, mitochondrial-mediated pathways.
CANCER GENE THERAPY
(2022)
Article
Biology
Yun Zhang, Jing Zhao, Xueliang Wang, Yuan Tang, Shuwen Liu, Tingyi Wen
Summary: This study successfully engineered Corynebacterium glutamicum ATCC13032 for the fermentation production of GABA and butyrolactam from glucose. The metabolic engineering strategies, including the construction of biosynthetic pathways and genetic modifications, effectively increased the production of GABA and butyrolactam. This research provides new insights into utilizing industrial microorganisms to produce target chemicals from renewable carbon sources.
Article
Biochemical Research Methods
Lai Li, Aihua Deng, Shuwen Liu, Junyue Wang, Ruilin Shi, Tiantian Wang, Di Cui, Hua Bai, Yun Zhang, Tingyi Wen
Summary: A major challenge in engineering microorganisms for desirable product is the balance between cell growth and product synthesis. In this study, researchers developed a method to screen and evaluate different quorum sensing systems and achieved efficient product synthesis in microorganisms. This approach can be widely applied in various microorganisms.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Hui Jian, Yun Zhang, Junyue Wang, Zhenxiang Chen, Tingyi Wen
Summary: This study proposes a strategy to enhance the effect of chemodynamic therapy (CDT) by targeting abnormal glutamine metabolism in cancer cells. The researchers developed iron-doped zeolitic imidazolate frameworks (ZIF(Fe) NPs) to co-deliver a drug called telaglenastat (CB-839) and Fe2+ into the tumor. The inhibition of glutamine metabolism by CB-839 amplifies Fe2+-induced oxidative stress and reduces nucleotide pools, leading to enhanced cell-killing effect of CDT. In vivo assessments confirm the improved anticancer performance and biocompatibility of the developed nanoparticles.
Review
Oncology
Faiza Naz, Mengran Shi, Salvia Sajid, Zhao Yang, Changyuan Yu
Summary: Cancer is characterized by heterogeneity, primarily caused by cancer stem cells that can be transformed by both intrinsic and extrinsic factors to intensify the aggressiveness of cancer cells. Understanding the intrinsic and extrinsic factors that influence cancer stem cell heterogeneity is crucial for intervening in these processes to ultimately improve cancer research and management.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)