Review
Chemistry, Physical
Ning-Bo Yi, Xing-Jie Hu, Fei Wang, Si-Yi Chen, Xiao-Xue Xu, Xin Li, Hao Wang, Taolei Sun, Zeng-Ying Qiao, Dong-Bing Cheng
Summary: Formaldehyde, the simplest endogenous carbonyl molecule, plays important roles in biosynthesis and metabolism. Detecting endogenous formaldehyde is crucial for disease diagnosis and therapeutic monitoring. Recent studies have focused on the development of formaldehyde-responsive fluorescent probes, which have great potential in the detection and visualization of formaldehyde in living systems and disease diagnostics. This review summarizes the fluorescence luminescence mechanism and design concepts of formaldehyde fluorescent probes, as well as their recent applications in bioimaging and illness diagnostics, providing references for further development in this field.
Article
Biochemical Research Methods
Jun Liu, Kaipeng Li, Peng Xue, Jinyi Xu
Summary: This study developed a new cell-permeable fluorescence probe functionality for visualizing endogenous formaldehyde changes. A smart multi-lock system was also proposed to monitor formaldehyde. The versatile latent fluorophore can be used to investigate the roles and functions of formaldehyde.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Yin Jiang, Shumei Huang, Minghui Liu, Zejun Li, Weimin Xiao, Huatang Zhang, Liu Yang, Hongyan Sun
Summary: Formaldehyde is involved in physiological processes but can also cause diseases if its levels are unbalanced. Fluorescent probes based on the 2-aza-Cope rearrangement mechanism have been widely used for imaging FA. FA-2, a probe with high sensitivity and selectivity, has been successfully used to monitor FA fluctuations within living cells.
Article
Materials Science, Biomaterials
Qi Song, Zhiqiang Liu, Jie Niu, Bowen Zheng, Jingcheng Hao, Jie Jiang
Summary: A two-photon excited fluorescent probe CMB-1 has been developed for the detection and regeneration of formaldehyde. It utilizes a novel nucleophilic addition and alcoholysis reactivity mechanism, enabling a specific turn-on response towards formaldehyde and allowing monitoring of both exogenous and endogenous formaldehyde in living cells through one- and two-photon microscopy, with minimal impact on its native homeostasis and local concentration.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Analytical
Wenlong Sheng, Xue Zhang, Miaohui Yu, Meng Jin, Ning Li, Chen Sun, Lizhen Wang, Qing Xia, Xiaobin Li, Yun Zhang, Baocun Zhu, Kechun Liu
Summary: A novel fluorescent probe Mem-FA was developed to sensitively detect and distinguish internal and external sources of formaldehyde on cell membrane, showing potential for specific tracing of formaldehyde-induced diseases.
Article
Chemistry, Applied
Zhangtao Min, Mai Zhang, Haijun Sun, Li Xu, Xiaoqing Wang, Zhipeng Liu
Summary: In this paper, a near-infrared fluorescent probe named DISE was reported, which showed a highly sensitive response to formaldehyde (FA). Confocal imaging experiments demonstrated that DISE can image endogenous and exogenous FA in Arabidopsis root tissue, indicating its potential as an imaging tool to study the biological roles of FA in plants.
Article
Biochemistry & Molecular Biology
Hui Sun, Qingyu Xu, Mingguang Ren, Fangong Kong
Summary: Formaldehyde (HCHO) is a common environmental toxicant that can harm the human respiratory tract and nervous system when exposed for a long period of time. HCHO is a carcinogen and increases the risk of cancer in humans. An innovative chitosan-based fluorescent probe (CS-FA) was developed for the detection of HCHO, which showed faster response time and high sensitivity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Spectroscopy
Yuting He, Hao Wang, Xinhang Fang, Weibing Zhang, Jingyuan Zhang, Junhong Qian
Summary: Two fluorescent sensors with semicarbazide receptors at different positions of coumarin were synthesized. One sensor (CAA) showed fluorescence turn-on response to Cu2+ and inhibition of PET for FA, while the other sensor (CAB) exhibited a three-stage fluorescence response to Cu2+. CAA showed good selectivity for quantification of Cu2+ and FA as well as bioimaging of Cu2+ in living cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Organic
Nai-Jie Lin, Huan Wu, Jing Peng, Shu-Han Yang, Rui Tan, Yu Peng, Ya-Wen Wang
Summary: A novel ratiometric probe SWJT-10 based on isophorone derivatives was successfully designed and synthesized for the detection of formaldehyde (FA). This probe exhibited significant ratiometric fluorescence response to FA in aqueous solution, with a blue shift from NIR (680 nm) to yellow light region (600 nm). It showed excellent selectivity, high sensitivity, and rapid response to FA (less than 5 s) due to a new recognition mechanism. Furthermore, SWJT-10 has been successfully applied for monitoring FA in living cells and zebrafish.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Chemistry, Physical
Lin Liu, Di Zhang, Man Li, Jiacheng Shi, Fengqi Guo, Jie Guo, Tieliang Wang
Summary: A new fluorescent probe ZZY was developed and synthesized to reversibly recognize sulfur dioxide (SO2) and formaldehyde (FA) in mitochondria. The probe exhibited high selectivity, sensitivity, low cytotoxicity, and a detection limit of 0.447 μM for SO2. It also showed good biocompatibility and successfully performed reversible imaging of SO2 and FA in mitochondria of living cells.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Review
Chemistry, Multidisciplinary
Xiao Liu, Young-Tae Chang
Summary: This article outlines the concepts and mechanisms of different strategies for live cell discrimination and introduces their applications in cell-type discrimination. These strategies include protein-oriented, carbohydrate-oriented, DNA-oriented, gating-oriented, metabolism-oriented, and lipid-oriented approaches. Fluorescent probes can achieve live cell distinction by interacting with biomolecules, accumulating via transporters, and participating in metabolism.
CHEMICAL SOCIETY REVIEWS
(2022)
Article
Spectroscopy
Songtao Cai, Chang Liu, Jin Gong, Song He, Liancheng Zhao, Xianshun Zeng
Summary: The article introduces a novel fluorescence probe NP-Lyso for detecting and imaging formic acid (FA) in lysosomes, which shows promising features after rational design and synthesis, providing a potential approach for investigating the biofunctions of FA in living systems.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Min Wang, Xiao Zhang, Le Wang, Jingyi Zhang, Na Liu, Di Zhang
Summary: A novel fluorescent probe (FAP1) with lysosomal localization group was designed and synthesized for the detection of formaldehyde. The probe showed good fluorescence recognition performances for formaldehyde, including large Stokes shift (130 nm), specific selectivity, good anti-interference ability, and low minimum detection limit (16 nM). It is notable that the probe exhibited good biocompatibility and could be applied to the imaging detection of formaldehyde in lysosome.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Physical
Zhiqiang Xu, Xiaomeng Yang, Ziru Liu, Ming-Xing Zhang
Summary: A new synthetic method utilizing 4-sulfo-1,8-naphthalimide derivative was discovered to construct 4-hydrazino-1,8-naphthalimide derivative, which acts as a fluorescent probe for detecting formaldehyde. The probe has advantages such as easy preparation, turn-on fluorescence signal, specific selectivity, and high sensitivity, and is capable of imaging exogenous/endogenous formaldehyde in living cells and visualizing formaldehyde in lysosomes. The crystal structure of the reaction product further confirms the possible recognition mechanism, and the probe can also detect gaseous formaldehyde using prepared test paper.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2022)
Article
Chemistry, Applied
Yansong Wang, Xiaolong Sun, Qingxin Han, Tony D. James, Xuechuan Wang
Summary: The study proposes a fluorescence-based method for detecting formaldehyde by synthesizing a water-soluble probe FAP-1 and successfully using it for formaldehyde analysis in genuine leather samples. Compared with traditional HPLC and UV-Vis methods, the fluorescence method is more competitive and promising.
Review
Chemistry, Multidisciplinary
Lu Lu, Zhou-yue Wu, Xin Li, Feng Han
ACTA PHARMACOLOGICA SINICA
(2019)
Article
Chemistry, Analytical
Juan Cheng, Yong Ren, Yujie Huang, Xiaozhuan Li, Mingzhu Huang, Feng Han, Xingguang Liang, Xin Li
ANALYTICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Chang Wang, Shengdan Wang, Yuan Wang, Honghai Wu, Kun Bao, Rong Sheng, Xin Li
SCIENTIFIC REPORTS
(2020)
Article
Biophysics
Fangqin Wang, Xuefeng Jiang, Huaijiang Xiang, Ning Wang, Yunjing Zhang, Xi Yao, Ping Wang, Hao Pan, Lifang Yu, Yunfeng Cheng, Yongzhou Hu, Weiqiang Lin, Xin Li
Summary: Acute kidney injury (AKI) is common in hospital patients, and the lack of efficient early diagnosis leads to delayed treatment and high mortality. A near-infrared fluorophore with inherent kidney-targeting ability has been discovered, which can be used for non-intrusive interrogation of AKI-related biomarkers.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Engineering, Chemical
Lei Zhou, Yu Chen, Baihao Shao, Juan Cheng, Xin Li
Summary: Hydrogen sulfide, a colorless acidic gas with a rotten-egg smell, is not only an endogenous signaling molecule with important biological functions but also an elusive subject in physiology and pathology. Research enthusiasm for hydrogen sulfide remains strong, with fluorescence imaging technology playing a crucial role in studying its biology, facilitated by the development of fluorescent probes.
FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Feng Yan, Jianfeng Zhuang, Qian Yu, Zhangqi Dou, Xuefeng Jiang, Shuyu Tan, Yifeng Han, Xinyan Wu, Yi Zang, Cong Li, Jia Li, Huaijun Chen, Libin Hu, Xin Li, Gao Chen
Summary: Novel fluorescent probe design strategy targeting PDGFR beta successfully developed PDGFP 1 probe for accurate glioma imaging and grading. Experimental results demonstrated high selectivity and positive correlation with tumor grades in mouse models and patient samples, showing potential for clinical translation.
Article
Chemistry, Analytical
Rongrong Tao, Meihua Liao, Yuxiang Wang, Huan Wang, Yuhang Tan, Siyao Qin, Wenjing Wei, Chunzhi Tang, Xingguang Liang, Yifeng Han, Xin Li
Summary: The study re-engineered phenylhydrazines to create a selective, rapid, and reversible fluorescent probe named PFM309 for tracking formaldehyde flux. PFM309 identified the crucial role of ALDH2 in AD pathology, showcasing its potential as a target for AD drug development.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yong Ren, Lei Zhou, Xin Li
Summary: Novel strategies were developed to optimize the photophysical properties of organic fluorophores for imaging probes. By mimicking the excited-state intramolecular proton transfer (ESIPT) effect, a series of 2-(2-hydroxyphenyl)-benzothiazole (HBT) derivatives were designed by complexing the heteroatoms therein with a boron atom, resulting in significant red-shifted emission wavelengths, enhanced quantum yields, and minimal impact on molecular size. Notably, compounds 12B and 13B emitted in the near-infrared region, making them among the smallest organic structures with emission above 650 nm.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Lei Zhou, Xuan Zhang, Ying Dong, Yuan Pan, Jia Li, Yi Zang, Xin Li
Summary: In this study, we developed a strategy of biomarker combination triggered tandem activation to design senescence-specific fluorogenic probe PGal_FA, which enables the simultaneous detection of multiple senescent biomarkers in the same live cell. We demonstrated the improved selectivity of probe PGal_FA toward senescent cells compared to traditional single-biomarker-based probe and successfully used it to detect senescent cells in bleomycin-induced pulmonary fibrosis tissues.
Article
Medicine, Research & Experimental
Rongrong Tao, Ning Wang, Tianruo Shen, Yuhang Tan, Yong Ren, Wenjing Wei, Meihua Liao, Davin Tan, Chunzhi Tang, Nenggui Xu, Huan Wang, Xiaogang Liu, Xin Li
Summary: By conducting computational modelling and fine-tuning the intramolecular charge transfer of Thioflavin T (ThT) analogues, researchers developed an ultrasensitive probe AH-2, which can image amyloid-beta (A beta) deposits in naturally aging mouse brains with high fidelity and has potential for early diagnosis of Alzheimer's disease (AD).
Article
Chemistry, Multidisciplinary
Rui-Ying Guo, Yu-Tian Zhang, Supphachok Chanmungkalakul, Hao-Ran Guo, Yongzhou Hu, Jia Li, Xiaogang Liu, Yi Zang, Xin Li
Summary: Inspired by the cellular machinery of reversible thiol modification by NO, a family of cysteine analogues tagged with fluorophores have been successfully designed to visualize cellular NO dynamics. Probes 1a and 2a were found to be biocompatible and capable of swiftly responding to cellular NO dynamics, making them promising tools for studying NO pathophysiology in health and disease.
Article
Nanoscience & Nanotechnology
Rui-Ying Guo, Han-Min Wang, Xiaowu Dong, Yongzhou Hu, Jia Li, Yi Zang, Xin Li
Summary: Fluorescence probes are attractive tools for tumor imaging, and two strategies are commonly used for designing tumor-targeted probes, with both showing similar tumor selectivity but the activatable probes exhibiting enhanced tumor-to-normal imaging contrast.
ACS APPLIED BIO MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Juan Cheng, Dan Li, Meiling Sun, Yi Wang, Qiao-Qin Xu, Xing-Guang Liang, Yun-Bi Lu, Yongzhou Hu, Feng Han, Xin Li
Article
Medicine, Research & Experimental
Yu Lei, Wenming Ren, Cheng-Kun Wang, Rong-Rong Tao, Huai-Jiang Xiang, Li-Li Feng, Yin-Ping Gao, Quan Jiang, Xin Li, Youhong Hu, Feng Han
Article
Medicine, Research & Experimental
Ruiying Guo, Feng Huang, Bo Zhang, Youyou Yan, Jinxin Che, Yizhen Jin, Yuxin Zhuang, Rong Dong, Yangling Li, Biqin Tan, Rui Song, Yongzhou Hu, Xiaowu Dong, Xin Li, Nengming Lin
