期刊
SCIENTIFIC REPORTS
卷 7, 期 -, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41598-017-16570-4
关键词
-
资金
- MRC [MR/K015885/1]
- University of Manchester
- A*STAR IMB
- BBSRC [BB/H017828/1, BB/N006933/1] Funding Source: UKRI
- MRC [MR/K015885/1] Funding Source: UKRI
The endoplasmic reticulum (ER) is a single organelle in eukaryotic cells that extends throughout the cell and is involved in a large number of cellular functions. Using a combination of fixed and live cells (human MRC5 lung cells) in diffraction limited and super-resolved fluorescence microscopy (STORM) experiments, we determined that the average persistence length of the ER tubules was 3.03 +/- 0.24 mu m. Removing the branched network junctions from the analysis caused a slight increase in the average persistence length to 4.71 +/- 0.14 mu m, and provides the tubule's persistence length with a moderate length scale dependence. The average radius of the tubules was 44.1 +/- 3.2 nm. The bending rigidity of the ER tubule membranes was found to be 10.9 +/- 1.2 kT (17.0 +/- 1.3 kT without branch points). We investigated the dynamic behaviour of ER tubules in live cells, and found that the ER tubules behaved like semi-flexible fibres under tension. The majority of the ER tubules experienced equilibrium transverse fluctuations under tension, whereas a minority number of them had active super-diffusive motions driven by motor proteins. Cells thus actively modulate the dynamics of the ER in a well-defined manner, which is expected in turn to impact on its many functions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据