期刊
SCIENTIFIC REPORTS
卷 7, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-12086-z
关键词
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资金
- European Union's Horizon European Research Council (ERC Consolidator Grant) [682574]
- National Institutes of Health [EB 015908, RR02584, HL 034557]
- Cancer Prevention and Research Institute of Texas (CPRIT grants) [RP 101243, RP 140021]
- Swiss National Science Foundation [PP00P2_133562]
- Centre d'Imagerie BioMedicale (CIBM) of the UNIL
- Centre d'Imagerie BioMedicale (CIBM) of the UNIGE
- Centre d'Imagerie BioMedicale (CIBM) of the HUG
- Centre d'Imagerie BioMedicale (CIBM) of the CHUV
- Centre d'Imagerie BioMedicale (CIBM) of the EPFL
- Leenards Foundation
- Jeantet Foundation
- European Research Council (ERC) [682574] Funding Source: European Research Council (ERC)
The mammalian brain relies primarily on glucose as a fuel to meet its high metabolic demand. Among the various techniques used to study cerebral metabolism, C-13 magnetic resonance spectroscopy (MRS) allows following the fate of C-13-enriched substrates through metabolic pathways. We herein demonstrate that it is possible to measure cerebral glucose metabolism in vivo with sub-second time resolution using hyperpolarized C-13 MRS. In particular, the dynamic C-13-labeling of pyruvate and lactate formed from C-13-glucose was observed in real time. An ad-hoc synthesis to produce [2,3,4,6,6-H-2(5), 3,4-C-13(2)]-D-glucose was developed to improve the 13C signal-to-noise ratio as compared to experiments performed following [U-H-2(7), U-C-13]-D-glucose injections. The main advantage of only labeling C3 and C4 positions is the absence of C-13-C-13 coupling in all downstream metabolic products after glucose is split into 3-carbon intermediates by aldolase. This unique method allows direct detection of glycolysis in vivo in the healthy brain in a noninvasive manner.
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