期刊
EUROPEAN JOURNAL OF MEDICAL GENETICS
卷 58, 期 2, 页码 95-104出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejmg.2014.12.001
关键词
Schizophrenia (SCZ); Epigenetic; DNA methylation; Long noncoding RNAs (lncRNAs)
资金
- National Natural Science Foundation of China [31100919, 81371469, 31301084]
- Natural Science Foundation of Zhejiang Province [LR13H020003, LQ13C060002]
- Natural Science Foundation of Ningbo [2013A610249]
- K. C. Wong Magna Fund in Ningbo University
- Ningbo social development research projects [2012C50032]
Schizophrenia (SCZ) is a complex mental disorder contributed by both genetic and epigenetic factors. Long noncoding RNAs (lncRNAs) was recently found playing an important regulatory role in mental disorders. However, little was known about the DNA methylation of lncRNAs, although numerous SCZ studies have been performed on genetic polymorphisms or epigenetic marks in protein coding genes. We presented a comprehensive genome wide DNA methylation study of both protein coding genes and lncRNAs in female patients with paranoid and undifferentiated SCZ. Using the methyl-CpG binding domain (MBD) protein-enriched genome sequencing (MBD-seq), 8,163 and 764 peaks were identified in paranoid and undifferentiated SCZ, respectively (p < 1 x 10-5). Gene ontology analysis showed that the hypermethylated regions were enriched in the genes related to neuron system and brain for both paranoid and undifferentiated SCZ (p < 0.05). Among these peaks, 121 peaks were located in gene promoter regions that might affect gene expression and influence the SCZ related pathways. Interestingly, DNA methylation of 136 and 23 known lncRNAs in Refseq database were identified in paranoid and undifferentiated SCZ, respectively. In addition. similar to 20% of intergenic peaks annotated based on Refseq genes were overlapped with lncRNAs in UCSC and gencode databases. In order to show the results well for most biological researchers, we created an online database to display and visualize the information of DNA methyation peaks in both types of SCZ (http://www.bioinfo.org/scz/scz.htm). Our results showed that the aberrant DNA methylation of lncRNAs might be another important epigenetic factor for SCZ. Crown Copyright (C) 2014 Published by Elsevier Masson SAS. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据