Article
Multidisciplinary Sciences
Masaki Kinoshita, Meng Amy Li, Michael Barber, William Mansfield, Sabine Dietmann, Austin Smith
Summary: Genome remethylation is crucial for mammalian development, with a study showing that deficiencies in Dnmt3a and Dnmt3b lead to ES cells developing into trophoblast cells due to failed suppression of Ascl2. Regulating the expression of Ascl2 can impact the transdifferentiation and contribution of ES cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Silvia Mara Baez Rodriguez, Georgiana-Adeline Staicu, Ani-Simona Sevastre, Carina Baloi, Vasile Ciubotaru, Anica Dricu, Ligia Gabriela Tataranu
Summary: Glioblastoma stem cells (GSCs) are self-renewing cells that can initiate tumors and are linked to tumor heterogeneity. Standard treatments fail to eradicate GSCs, leading to disease recurrence. Understanding the biology and survival mechanisms of GSCs is crucial for targeting them effectively.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Karina Biserova, Arvids Jakovlevs, Romans Uljanovs, Ilze Strumfa
Summary: Cancer stem cells, particularly in glioblastoma, play a crucial role in tumor resistance to treatment and recurrence. The regulatory mechanisms of glioma stem cells include tumor microenvironment, niche concept, metabolism, immunity, genetics, and epigenetics, which serve as potential targets for future treatments.Understanding the molecular markers and treatment resistance of GSCs in glioblastoma may lead to more effective therapeutic strategies in the future.
Article
Oncology
Lei Qiu, Xiuwei Yang, Jingyu Wu, Changzhi Huang, Yongchang Miao, Zan Fu
Summary: The study reveals that upregulation of HIST2H2BF enhances CSC phenotype, malignancy, and liver metastasis through activating Notch signaling in CRC, shedding light on the mechanism of maintaining stem cell features in CRC and providing new potential therapeutic targets.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Steffi Urbschat, Gerrit Breitfelder, Mohamed Henia, Walter Schulz-Schaeffer, Christoph Sippl, Joachim Oertel, Ralf Ketter
Summary: This study analyzed the methylation status of the RB1 promoter in 85 glioblastomas and found only one case with RB1 promoter methylation. Despite contradictory results in the literature, this is the largest study on this topic to date and the first to use the WHO 2016 classification, suggesting that RB1 promoter methylation does not play a role in the development and progression of glioblastoma.
Article
Multidisciplinary Sciences
Claire Vinel, Gabriel Rosser, Loredana Guglielmi, Myrianni Constantinou, Nicola Pomella, Xinyu Zhang, James R. Boot, Tania A. Jones, Thomas O. Millner, Anaelle A. Dumas, Vardhman Rakyan, Jeremy Rees, Jamie L. Thompson, Juho Vuononvirta, Suchita Nadkarni, Tedani El Assan, Natasha Aley, Yung-Yao Lin, Pentao Liu, Sven Nelander, Denise Sheer, Catherine L. R. Merry, Federica Marelli-Berg, Sebastian Brandner, Silvia Marino
Summary: This study compares patient-matched glioma-initiating cells with neural stem cells, enabling the discovery of patient-specific disease mechanisms and identification of effective drugs.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Joana Vieira de Castro, Celine S. Goncalves, Eduarda P. Martins, Irene Miranda-Lorenzo, Mariana T. Cerqueira, Adhemar Longatto-Filho, Afonso A. Pinto, Rui L. Reis, Nuno Sousa, Christopher Heeschen, Bruno M. Costa
Summary: Identifying and isolating glioblastoma stem cells (GSCs) is crucial for understanding tumor biology and developing targeted therapies. A novel subpopulation of autofluorescent cells in GBM has been discovered with all the functional characteristics of GSCs, providing a simple and reliable biomarker for targeting these highly malignant tumors. This discovery has significant implications for both biological and clinical research in glioblastoma.
Review
Cell Biology
Raul Fernandez Perez, Juan Ramon Tejedor, Agustin Fernandez Fernandez, Mario Fernandez Fraga
Summary: Aging and cancer are clearly associated processes, and epigenetic mechanisms play a role in connecting the two phenomena. However, recent studies have shown significant differences in DNA methylation loss between aging and cancer. The availability of genome-wide epigenetic data allows for systematic studies to clarify the similarities and differences in epigenetic alterations between the two processes. Understanding the molecular mechanisms underlying epigenetic clocks, especially in somatic stem cell aging, and integrating existing and upcoming epigenetic evidence within genetic damage models of aging will be crucial for better understanding of these interrelated phenomena.
Article
Cell Biology
Cristina Pagano, Giovanna Navarra, Laura Coppola, Giorgio Avilia, Olga Pastorino, Rosa Della Monica, Michela Buonaiuto, Giovanni Torelli, Pasquale Caiazzo, Maurizio Bifulco, Chiara Laezza
Summary: This study demonstrates that N6-isopentenyladenosine (iPA) induces caspase-independent necroptosis in glioblastoma cells, bypassing the apoptosis resistance mechanism and potentially offering a more effective therapeutic strategy.
CELL DEATH DISCOVERY
(2022)
Review
Oncology
Manam Inushi De Silva, Brett W. Stringer, Cedric Bardy
Summary: Glioblastoma (GBM) is the most lethal primary brain cancer due to treatment-resistant recurrence. Recent studies suggest that GBM tumor cells exhibit heterogeneous populations originating from unique genetic abnormalities, which allows them to adapt and evade treatment. It is proposed that effective treatment of GBM requires therapeutics that restrain GBM plasticity, considering the boundaries of tumorigenesis, neurodevelopment, and brain injury in GBM.
Article
Multidisciplinary Sciences
Hiroshi Sakai, Yuichiro Sawada, Naohito Tokunaga, Kaori Tanaka, So Nakagawa, Iori Sakakibara, Yusuke Ono, So-Ichiro Fukada, Yasuyuki Ohkawa, Tadahiko Kikugawa, Takashi Saika, Yuuki Imai
Summary: DNA methylation is crucial for regulating cellular identity, and the epigenetic regulator Uhrf1 plays a critical role in the proliferation and differentiation of satellite cells in muscle regeneration by controlling cell-type-specific gene expression through maintenance of DNA methylation.
Article
Oncology
Sabah Akhtar, Shireen Hourani, Lubna Therachiyil, Abdullah Al-Dhfyan, Abdelali Agouni, Asad Zeidan, Shahab Uddin, Hesham M. Korashy
Summary: This article summarizes the characteristics and important roles of cancer stem cells (CSCs) and their connection with environmental toxin PAHs and cytosolic receptor AhR. The review also discusses the signaling pathways related to tumorigenesis and progression that are mediated by AhR, as well as the epigenetic regulations of CSCs by the AhR/CYP1A pathway.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Cell Biology
Chuanyu Zhang, Hang Wen, Siying Liu, Enze Fu, Lu Yu, Shang Chen, Qingsheng Han, Zongjin Li, Na Liu
Summary: This study identifies a regulator called Dppa3 that promotes the formation of 2CLCs and zygotic genome activation. Dppa3 regulates DNA methylation in ESCs and its overexpression leads to the conversion of ESCs into 2CLCs, which is associated with DNA damage response.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Joana B. B. Serrano, Nils C. C. Tabeling, Cindy M. M. de Winter-Korver, Saskia K. M. van Daalen, Ans M. M. van Pelt, Callista L. L. Mulder
Summary: This study investigates the DNA methylation status of sperm from SSCT-derived offspring in F1 and F2 generations using reduced-representation bisulfite sequencing. The results show no major differences in DNA methylation between SSCT-derived offspring and control. These findings provide crucial support for the clinical application of SSCT in humans.
CLINICAL EPIGENETICS
(2023)
Article
Biochemistry & Molecular Biology
Jun Tang, Weiyan Peng, Yixiao Feng, Xin Le, Kang Wang, Qin Xiang, Lili Li, Yan Wang, Can Xu, Junhao Mu, Ke Xu, Ping Ji, Qian Tao, Ailong Huang, Chu-Xia Deng, Yong Lin, Tingxiu Xiang
Summary: This study discusses the importance of inactivation of tumor-suppressor genes in oncogenesis, as well as the role of a novel epigenetic regulatory mechanism in p53 inactivation.
Article
Neurosciences
Karolina Minta, Gunnar Brinkmalm, Erik Portelius, Per Johansson, Johan Svensson, Petronella Kettunen, Anders Wallin, Henrik Zetterberg, Kaj Blennow, Ulf Andreasson
Summary: The study identified that the concentrations of brevican and neurocan peptides were significantly decreased in the cerebrospinal fluid of VaD patients, suggesting their potential as diagnostic biomarkers for VaD.
JOURNAL OF ALZHEIMERS DISEASE
(2021)
Article
Clinical Neurology
Liu Shi, Laura M. Winchester, Sarah Westwood, Alison L. Baird, Sneha N. Anand, Noel J. Buckley, Abdul Hye, Nicholas J. Ashton, Isabelle Bos, Stephanie J. B. Vos, Mara ten Kate, Philip Scheltens, Charlotte E. Teunissen, Rik Vandenberghe, Silvy Gabel, Karen Meersmans, Sebastiaan Engelborghs, Ellen E. De Roeck, Kristel Sleegers, Giovanni B. Frisoni, Olivier Blin, Jill C. Richardson, Regis Bordet, Jose L. Molinuevo, Lorena Rami, Anders Wallin, Petronella Kettunen, Magda Tsolaki, Frans Verhey, Alberto Lleo, Isabel Sala, Julius Popp, Gwendoline Peyratout, Pablo Martinez-Lage, Mikel Tainta, Peter Johannsen, Yvonne Freund-Levi, Lutz Froeich, Valerija Dobricic, Cristina Legido-Quigley, Frederik Barkhof, Ulf Andreasson, Kaj Blennow, Henrik Zetterberg, Johannes Streffer, Christina M. Lill, Lars Bertram, Pieter Jelle Visser, Hartmuth C. Kolb, Vaibhav A. Narayan, Simon Lovestone, Alejo J. Nevado-Holgado
Summary: The study found that blood proteins can predict the presence of AD pathology as measured in the ATN framework as well as clinical diagnosis.
ALZHEIMERS & DEMENTIA
(2021)
Article
Biochemistry & Molecular Biology
Anirban Das, Sumedha Sudhaman, Daniel Morgenstern, Ailish Coblentz, Jiil Chung, Simone C. Stone, Noor Alsafwani, Zhihui Amy Liu, Ola Abu Al Karsaneh, Shirin Soleimani, Hagay Ladany, David Chen, Matthew Zatzman, Vanja Cabric, Liana Nobre, Vanessa Bianchi, Melissa Edwards, Lauren C. Sambira Nahum, Ayse B. Ercan, Arash Nabbi, Shlomi Constantini, Rina Dvir, Michal Yalon-Oren, Gadi Abebe Campino, Shani Caspi, Valerie Larouche, Alyssa Reddy, Michael Osborn, Gary Mason, Scott Lindhorst, Annika Bronsema, Vanan Magimairajan, Enrico Opocher, Rebecca Loret De Mola, Magnus Sabel, Charlotta Frojd, David Sumerauer, David Samuel, Kristina Cole, Stefano Chiaravalli, Maura Massimino, Patrick Tomboc, David S. Ziegler, Ben George, An Van Damme, Nobuko Hijiya, David Gass, Rose B. McGee, Oz Mordechai, Daniel C. Bowers, Theodore W. Laetsch, Alexander Lossos, Deborah T. Blumenthal, Tomasz Sarosiek, Lee Yi Yen, Jeffrey Knipstein, Anne Bendel, Lindsey M. Hoffman, Sandra Luna-Fineman, Stefanie Zimmermann, Isabelle Scheers, Kim E. Nichols, Michal Zapotocky, Jordan R. Hansford, John M. Maris, Peter Dirks, Michael D. Taylor, Abhaya Kulkarni, Manohar Shroff, Derek S. Tsang, Anita Villani, Wei Xu, Melyssa Aronson, Carol Durno, Adam Shlien, David Malkin, Gad Getz, Yosef E. Maruvka, Pamela S. Ohashi, Cynthia Hawkins, Trevor J. Pugh, Eric Bouffet, Uri Tabori
Summary: This study reports on the effectiveness of ICI treatment in children with tumors caused by germline DNA replication repair deficiency. It shows that high mutation burden and microsatellite load are important predictors of sustained response and long-term survival following PD-1 blockade.
Article
Endocrinology & Metabolism
Tobias Hallen, Gudmundur Johannsson, Rahil Dahlen, Camilla A. M. Glad, Charlotte Orndal, Angelica Engvall, Helena Caren, Thomas Skoglund, Daniel S. Olsson
Summary: This study explored DNA methylation patterns associated with tumor progression in patients with nonfunctioning pituitary adenomas (NFPAs). The results showed that some methylation sites were associated with tumor progression and could potentially serve as biomarkers for early identification of tumor progression.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Neurosciences
Susanna Larsson, Petronella Kettunen, Helena Caren
Summary: Brain tumours are a leading cause of death in children with solid tumours, and high-grade gliomas are particularly devastating. This study presents a clinically relevant in vivo model for paediatric glioma using zebrafish larvae and primary glioma stem cell cultures. The model allows for the monitoring of tumour invasion and evaluation of drug treatments. This research has the potential to improve understanding of cellular behaviour and personalized treatments for paediatric high-grade gliomas.
Article
Biology
Marica Andersson, Jonathan A. C. Roques, Geoffrey Mukisa Aliti, Karin Ademar, Henrik Sundh, Kristina Sundell, Mia Ericson, Petronella Kettunen
Summary: The housing density of zebrafish affects their welfare, with a density of 1 fish/L leading to higher stress levels and aggressive behavior. However, densities between 4 and 16 fish/L do not compromise the welfare of zebrafish.
Article
Clinical Neurology
Jonathan Kjaer Gronbaek, Aske Foldbjerg Laustsen, Sebastian Toescu, Barry Pizer, Conor Mallucci, Kristian Aquilina, Emanuela Molinari, Magnus Aasved Hjort, Lingvita Gumbeleviciene, Peter Hauser, Beatrix Palmafy, Kirsten van Baarsen, Eelco Hoving, Julian Zipfel, Christoffer Ehrstedt, Pernilla Grillner, Michael Thude Callesen, Radek Fric, Morten Wibroe, Karsten Nysom, Kjeld Schmiegelow, Astrid Sehested, Rene Mathiasen, Marianne Juhler
Summary: According to the study, there is no significant relationship between handedness and postoperative speech impairment. The study does not support the hypothesis that left-handedness should be of clinical relevance in the risk assessment of CMS.
CHILDS NERVOUS SYSTEM
(2022)
Article
Pathology
Sandra Ferreyra Vega, Anna Wenger, Teresia Kling, Thomas Olsson Bontell, Asgeir Store Jakola, Helena Caren
Summary: Diffuse gliomas and meningiomas, the most common primary intracranial tumors of the central nervous system, exhibit genetic and epigenetic heterogeneity within the tumors, with potential clinical implications for diagnosis and assessment of molecular markers.
Article
Clinical Neurology
Elizabeth Schepke, Maja Lofgren, Torsten Pietsch, Thomas Olsson Bontell, Teresia Kling, Anna Wenger, Sandra Ferreyra Vega, Anna Danielsson, Sandor Dosa, Stefan Holm, Anders Oberg, Per Nyman, Marie Eliasson-Hofvander, Per-Erik Sandstrom, Stefan M. Pfister, Birgitta Lannering, Magnus Sabel, Helena Caren
Summary: DNA methylation analysis is a reliable method for the precise diagnosis of paediatric brain tumours, providing important molecular information for treatment decisions.
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
(2022)
Article
Oncology
Olof Rask, Fredrik Nilsson, Paivi Lahteenmaki, Christoffer Ehrstedt, Stefan Holm, Per-Erik Sandstrom, Per Nyman, Magnus Sabel, Pernilla Grillner
Summary: This study investigates the diagnostic time intervals for tumors of the central nervous system in children and adolescents in Sweden and identifies factors associated with delayed diagnosis. The results provide valuable insights for clinical improvement.
PEDIATRIC BLOOD & CANCER
(2022)
Article
Neurosciences
Anna Wenger, Sandra Ferreyra Vega, Elizabeth Schepke, Maja Lofgren, Thomas Olsson Bontell, Magnus Tisell, Daniel Nilsson, Teresia Kling, Helena Caren
Summary: DNA methylation is stable and promising for tumor classification in pediatric brain tumors, with more changes occurring in high-grade tumors and mainly in regions without gene affiliation.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2022)
Article
Oncology
Asimina Zisi, Dimitris C. Kanellis, Simon Moussaud, Ida Karlsson, Helena Caren, Lars Braeutigam, Jiri Bartek, Mikael S. Lindstrom
Summary: High-grade gliomas are aggressive and resistant to chemotherapy. Inhibition of ribosome biogenesis (RiBi) could be a potential therapeutic strategy for glioma, as it is positively correlated with the development of glioma.
Article
Neurosciences
Sandra Ferreyra Vega, Thomas Olsson Bontell, Teresia Kling, Asgeir Store Jakola, Helena Caren
Summary: This study investigated the DNA methylation changes associated with tumor progression in primary and recurrent IDH-mutant gliomas. It was found that a subset of IDH-mutant astrocytomas exhibited a switch in methylation subtypes, with the primary tumors classified as low-grade astrocytomas progressing to high-grade astrocytomas in the recurrent tumors. The number of differentially methylated CpG sites increased over time, and astrocytomas accumulated more differentially methylated CpG sites than oligodendrogliomas during tumor progression.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2023)
Review
Oncology
Anna Wenger, Helena Caren
Summary: Diffuse gliomas are brain tumors that affect adults and children. DNA methylation patterns can be used as a diagnostic tool for tumor classification and stratification, leading to changes in clinical diagnosis. The use of DNA methylation profiling as a diagnostic tool requires consideration of several factors.