期刊
ONCOTARGET
卷 8, 期 23, 页码 37342-37354出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.16954
关键词
calprotectin; S100A8/A9; food hypersensitivity; IgE; animal experiment
资金
- Shanghai Municipal Commission of Health and Family Planning [201640297]
- Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition [11DZ2260500]
- National Natural Science Foundation of China [81172686, 81302446]
Calprotectin, also known as S100A8/A9, has been linked to gut inflammation caused by IgE-mediated food hypersensitivities, but the pathophysiologic abnormalities it causes remain to be determined. We created a mild food hypersensitivity model through oral gavage of ovalbumin in Norway brown rats without using immune adjuvant. Changes in the levels of calprotectin and inflammation-associated cytokines were then observed over time. We found that fecal calprotectin as well as jejunal and liver TLR4, TNF-alpha, NF-kappa B, IL-1 beta, and IL-6 were upregulated in hypersensitive rats. Additionally, the influence of calprotectin on CD4(+) T and dendritic cells was observed by co-culturing CD4(+) T cells with dendritic cells, which revealed a shift toward increased Th2 T cells in calprotectin-treated cultures. These results suggest that calprotectin, along with other inflammatory factors, promotes the inflammation seen in mild food allergy.
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