Article
Entomology
Yayun Zuo, Zeyu Wang, Xuan Ren, Yakun Pei, Ahmed A. A. Aioub, Zhaonong Hu
Summary: miRNAs are small, non-coding RNAs that play crucial roles in regulating various biological processes. This study shows that the loss of a specific miRNA may not result in obvious phenotypic changes, suggesting redundancy in miRNA function. Transcriptome analysis reveals changes in global gene expression patterns in the miRNA knockout strain, indicating the potential role of miRNAs in transcriptional adaptation. These findings highlight the importance of studying miRNA function and its impact on gene expression.
Article
Biochemistry & Molecular Biology
Yu-Jen Chen, Chia-Tien Hsu, Shang-Feng Tsai, Cheng-Hsu Chen
Summary: Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. In this study, the correlation between plasma miR-21 and the severity of CAD was investigated. RNA sequencing was performed and differentially expressed miRNAs in CAD were identified. MiR-21-5p, miR-20a-5p, and miR-101-3p were found to be associated with the TGF-beta pathway and CAD. These findings may lead to the development of early prediction miRNA biomarkers and therapeutic tools for CAD in the field of kidney transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Jinhui Che, Zhan Su, Weizhong Yang, Lu Xu, Yunjiu Li, Haihong Wang, Wuyuan Zhou
Summary: This study investigated the regulatory mechanism of the p53/miR-29c-3p/ADAM12 axis in hepatocellular carcinoma (HCC) using CRISPR/Cas9 technology. The results showed that ADAM12 was highly expressed while miR-29c-3p was lowly expressed in HCC. miR-29c-3p inhibited the migratory and invasive abilities of HCC cells by targeting ADAM12 expression. Moreover, p53 upregulated miR-29c-3p, thereby suppressing HCC cell activities, and ADAM12 knockout or p53 overexpression reduced HCC tumor formation and metastasis.
DIGESTIVE AND LIVER DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Asmaa Y. Abuhamad, Nurul Nadia Mohamad Zamberi, Sakari Vanharanta, Siti Nur Hasanah Mohd Yusuf, M. Aiman Mohtar, Saiful Effendi Syafruddin
Summary: This study found that clear cell renal cell carcinoma (ccRCC) cells are able to secrete PDGFB, which can activate the mTORC1 signaling pathway and promote the development of ccRCC. The level of PDGFB secretion was positively correlated with the expression of intracellular KLF6 and PDGFB. Reintroduction of either KLF6 or PDGFB sustained mTORC1 signaling activity in KLF6-targeted ccRCC cells. Conditioned media of PDGFB-overexpressing ccRCC cells was able to re-activate mTORC1 activity in KLF6-targeted cells. Cancer cell-derived PDGFB can mediate mTORC1 signaling pathway activation in ccRCC, further solidifying the association between the KLF6-PDGFB axis and mTORC1 signaling pathway activity in ccRCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Yiguan Qian, Yang Li, Luwei Xu, Ke Chen, Ning Liu, Xiaobing Yang, Qian Lv, Rongfei Li, Changcheng Zhou, Zheng Xu, Ruipeng Jia, Yu-Zheng Ge
Summary: In this study, a novel circRNA called circ-PRKCI was found to promote the proliferation of renal cell carcinoma (RCC) through the miR-545-3p/CCND1 signaling pathway. It was also discovered that tumor cell-derived exosomal circ-PRKCI could enhance the proliferation, migration, and invasion of RCC cells while inhibiting apoptosis. This study provides new insights into the molecular mechanisms of RCC progression.
Article
Biotechnology & Applied Microbiology
Jonathan W. Villanueva, Lawrence Kwong, Teng Han, Salvador Alonso Martinez, Michael T. Shanahan, Matt Kanke, Lukas E. Dow, Charles G. Danko, Praveen Sethupathy
Summary: Somatic mutations play a crucial role in driving colorectal cancer (CRC) by disrupting gene regulatory mechanisms, and different combinations of mutations can lead to unique changes in regulatory mechanisms, affecting the efficacy of therapeutics. MicroRNAs, important regulators of gene expression, can be altered by oncogenic mutations. This study identified 12 different modules of microRNA expression patterns in genetically-modified mouse intestinal organoid models with different combinations of mutations common in CRC. The study also demonstrated the aberrant upregulation of miR-24-3p in genetically-modified mouse enteroids, regardless of mutational context. Furthermore, miR-24-3p was found to promote CRC cell survival in multiple cell contexts. These findings provide insight into the mechanisms driving inter-tumor heterogeneity and highlight potential microRNA therapeutic targets for precision medicine in CRC.
Article
Oncology
Patrice Cagle, Nikia Smith, Timothy O. Adekoya, Yahui Li, Susy Kim, Leslimar Rios-Colon, Gagan Deep, Suryakant Niture, Christopher Albanese, Simeng Suy, Sean P. Collins, Deepak Kumar
Summary: The abnormal expression of microRNA miR-214-3p plays a role in promoting the progression of prostate cancer, while miR-214 functions as a tumor suppressor. Targeting miR-214 may offer a new therapeutic approach for the treatment of prostate cancer.
Article
Medicine, Research & Experimental
Ziwei Li, Jie Wang, Dianting Li, Haiying Chen, Tao Meng
Summary: This study revealed that the expression levels of miR-372-3p were upregulated in placental tissues from patients with PE, especially in those with early-onset PE. In vitro analysis demonstrated that miR-372-3p overexpression inhibited trophoblast cell migration, invasion, and epithelial-mesenchymal transition (EMT). The study also found that miR-372-3p is sponged by twist1 and downregulating twist1 expression can rescue the inhibitory effects of miR-372-3p overexpression.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Andrology
Yi Ma, Yan Zhou, Qian Xiao, Sha-Sha Zou, Yin-Ci Zhu, Ping Ping, Xiang-Feng Chen
Summary: The study found that seminal exosomal miR-210-3p levels were positively correlated with the severity of varicocoele in patients, while negatively correlated with sperm count and seminal inhibin-B expression. Surgical treatment significantly reduced the level of miR-210-3p.
Article
Oncology
Dmitry Chernyakov, Alexander Gross, Annika Fischer, Nicola Bornkessel, Christoph Schultheiss, Dennis Gerloff, Bayram Edemir
Summary: This study reveals that NFAT5 directly regulates the expression of Ranbp3l by binding its promoter, leading to tumor-like phenotype and altered cell behavior and gene expression in RANBP3L-deficient cells. Loss of RANBP3L may promote the development and progression of renal cell carcinoma.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Cell Biology
Nianyong Yuan, Guowei Li
Summary: In this study, hsa_circ_0037858 was identified as a potential circRNA related to ccRCC metastasis. It was significantly downregulated in ccRCC and further decreased in metastatic ccRCC. MiR-5000-3p was predicted to be the most potential binding miRNA of hsa_circ_0037858. Protein-protein interaction analysis revealed several hub genes associated with miR-5000-3p, and FMR1 was identified as the most potential downstream gene. Hsa_circ_0037858 suppressed metastasis and enhanced FMR1 expression in ccRCC through the miR-5000-3p/FMR1 axis.
Article
Pharmacology & Pharmacy
Yuanjun Lu, Yau-Tuen Chan, Junyu Wu, Zixin Feng, Hongchao Yuan, Qiucheng Li, Tingyuan Xing, Lin Xu, Cheng Zhang, Hor-Yue Tan, Terence Kin-Wah Lee, Yibin Feng, Ning Wang
Summary: This study identified miR-3689a-3p as a critical microRNA responsible for sorafenib resistance in hepatocellular carcinoma (HCC). miR-3689a-3p disrupts intracellular copper trafficking by targeting CCS, resulting in reduced scavenging of mitochondrial oxidative stress and ultimately causing cell death in response to sorafenib treatment in HCC.
DRUG RESISTANCE UPDATES
(2023)
Article
Microbiology
Ata Garajei, Milad Parvin, Hady Mohammadi, Abdolamir Allameh, Azin Hamidavi, Masoud Sadeghi, Azadeh Emami, Serge Brand
Summary: This study evaluated the expression of miR-486-3p, miR-561-5p, miR-548-3p, and miR-509-5p in tissue biopsy samples with and without OSCC. The results showed that the expression of miR-486-3p and miR-561-5p was significantly lower in cancer samples compared to normal tissue samples, while miR-548-3p expression increased in the OSCC group compared to the control group. There was no significant difference in miR-509-5p expression between OSCC and control groups.
Article
Cell Biology
Bin Zhao, Cong Huang, Jie Pan, Hao Hu, Xiaojuan Liu, Kaoyuan Zhang, Fenli Zhou, Xin Shi, Jun Wu, Bo Yu, Xiaofan Chen, Wei Zhang
Summary: Recent evidence shows that circRNAs, particularly circPLIN2, play important regulatory roles in the development and progression of clear cell renal cell carcinoma (ccRCC) by increasing mRNA stability and competitively binding specific miRNA.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Ying Wang, Yunjing Zhang, Xinwan Su, Qiongzi Qiu, Yuan Yuan, Chunhua Weng, Sailan Zou, Yan Tian, Weidong Han, Pengyuan Liu, Xingyi Guo, Jianhua Mao, Xianghui Fu, Ping Wang, Weiqiang Lin
Summary: This study found that circDVL1 is reduced in the serums and tissues from ccRCC patients, and negatively correlates with malignant features of ccRCC. Overexpression of circDVL1 inhibits proliferation and migration, induces apoptosis in ccRCC cells. Mechanistically, circDVL1 functions as a sponge for miR-412-3p, preventing its repression of target gene PCDH7 in ccRCC cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Oncology
Zhiyong Wang, Xiaodong Feng, Alfredo A. Molinolo, Daniel Martin, Lynn Vitale-Cross, Nijiro Nohata, Mizuo Ando, Amy Wahba, Panomwat Amornphimoltham, Xingyu Wu, Mara Gilardi, Michael Allevato, Victoria Wu, Dana J. Steffen, Philip Tofilon, Nahum Sonenberg, Joseph Califano, Qianming Chen, Scott M. Lippman, J. Silvio Gutkind
Article
Oncology
Damiano Cosimo Rigiracciolo, Maria Francesca Santolla, Rosamaria Lappano, Adele Vivacqua, Francesca Cirillo, Giulia Raffaella Galli, Marianna Talia, Lucia Muglia, Michele Pellegrino, Nijiro Nohata, Maria Teresa Di Martino, Marcello Maggiolini
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2019)
Article
Oncology
Hiroko Toda, Naohiko Seki, Sasagu Kurozumi, Yoshiaki Shinden, Yasutaka Yamada, Nijiro Nohata, Shogo Moriya, Tetsuya Idichi, Kosei Maemura, Takaaki Fujii, Jun Horiguchi, Yuko Kijima, Shoji Natsugoe
MOLECULAR ONCOLOGY
(2020)
Article
Oncology
Yasutaka Yamada, Nijiro Nohata, Akifumi Uchida, Mayuko Kato, Takayuki Arai, Shogo Moriya, Keiko Mizuno, Satoko Kojima, Kazuto Yamazaki, Yukio Naya, Tomohiko Ichikawa, Naohiko Seki
Article
Oncology
Hirofumi Yoshino, Hideki Enokida, Yoichi Osako, Nijiro Nohata, Masaya Yonemori, Satoshi Sugita, Kazuki Kuroshima, Masafumi Tsuruda, Shuichi Tatarano, Masayuki Nakagawa
MOLECULAR ONCOLOGY
(2020)
Article
Cell Biology
Damiano Cosimo Rigiracciolo, Nijiro Nohata, Rosamaria Lappano, Francesca Cirillo, Marianna Talia, Domenica Scordamaglia, J. Silvio Gutkind, Marcello Maggiolini
Article
Cell Biology
Keiko Mizuno, Kengo Tanigawa, Nijiro Nohata, Shunsuke Misono, Reona Okada, Shunichi Asai, Shogo Moriya, Takayuki Suetsugu, Hiromasa Inoue, Naohiko Seki
Article
Oncology
Shunsuke Misono, Keiko Mizuno, Takayuki Suetsugu, Kengo Tanigawa, Nijiro Nohata, Akifumi Uchida, Hiroki Sanada, Reona Okada, Shogo Moriya, Hiromasa Inoue, Naohiko Seki
Summary: Small cell lung cancer (SCLC) is a fatal tumor with poor prognosis in patients who relapse after initial treatment. This study identified a molecular signature of SCLC after treatment failure, focusing on genes related to the cell cycle pathway. The overexpression of MCM2, MCM4, MCM6, and MCM7 was detected in SCLC clinical specimens, suggesting their potential as therapeutic targets. Knockdown of these MCM genes attenuated cancer cell proliferation and enhanced cisplatin sensitivity in SCLC cells, indicating their role in treatment resistance.
Article
Oncology
Damiano Cosimo Rigiracciolo, Nijiro Nohata, Rosamaria Lappano, Francesca Cirillo, Marianna Talia, Sendi Rafael Adame-Garcia, Nadia Arang, Simone Lubrano, Ernestina Marianna De Francesco, Antonino Belfiore, J. Silvio Gutkind, Marcello Maggiolini
Summary: This study aimed to explore the function of the S100A8/A9-RAGE system in TNBC. The results showed that S100A8 and S100A9 were highly expressed in BC, particularly in HER2-positive and TNBC, and were associated with poor clinical outcomes. High RAGE expression was also correlated with poor overall survival in BC patients. The study further revealed that the S100A8/A9-RAGE system triggers FAK activation by engaging a cytoskeleton mechanosensing complex in TNBC cells, and identified the Hippo pathway as the most enriched in BC patients expressing high RAGE levels. The findings suggest that the RAGE-FAK-YAP transduction pathway could be a potential target for halting the aggressive TNBC subtype.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Shunichi Asai, Ayaka Koma, Nijiro Nohata, Takashi Kinoshita, Naoko Kikkawa, Mayuko Kato, Chikashi Minemura, Katsuhiro Uzawa, Toyoyuki Hanazawa, Naohiko Seki
Summary: Based on RNA sequence analysis, the expression of miR-1-3p, miR-206, miR-133a-3p, and miR-133b was found to be suppressed in HNSCC. In silico analysis identified 28 genes that were potentially regulated by these miRNAs and were upregulated in HNSCC tissues. The expression of PFN2 and PSEN1 was found to be independent prognostic factors for HNSCC patients, and miR-1-3p, miR-206, miR-133a-3p, and miR-133b directly controlled PFN2 expression, affecting the migration and invasion abilities of cancer cells.
Article
Biochemistry & Molecular Biology
Tomoaki Saito, Shunichi Asai, Nozomi Tanaka, Nijiro Nohata, Chikashi Minemura, Ayaka Koma, Naoko Kikkawa, Atsushi Kasamatsu, Toyoyuki Hanazawa, Katsuhiro Uzawa, Naohiko Seki
Summary: This study utilized ChIP-Seq to analyze active enhancers and identified genes associated with drug resistance and prognosis in oral squamous cell carcinoma, providing crucial genetic information for improving treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Shunji Takahashi, Nobuhiko Oridate, Kaoru Tanaka, Yasushi Shimizu, Yasushi Fujimoto, Koji Matsumoto, Tomoya Yokota, Tomoko Yamazaki, Masanobu Takahashi, Tsutomu Ueda, Nobuhiro Hanai, Hironori Yamaguchi, Hiroki Hara, Tomokazu Yoshizaki, Ryuji Yasumatsu, Masahiro Nakayama, Kiyoto Shiga, Takashi Fujii, Kenji Mitsugi, Kenichi Takahashi, Nijiro Nohata, Burak Gumuscu, Ramona F. Swaby, Makato Tahara
Summary: The study reports the efficacy of pembrolizumab in Japanese patients with R/M HNSCC, suggesting it as a first-line treatment option. Treatment-related adverse events occurred at a relatively high rate, but the overall safety profile was acceptable.
INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Alimasi Aersilan, Naoko Hashimoto, Kazuyuki Yamagata, Masataka Yokoyama, Akitoshi Nakayama, Xiaoyan Shi, Hidekazu Nagano, Ikki Sakuma, Nijiro Nohata, Takashi Kinoshita, Naohiko Seki, Bahityar Rahmutulla, Atsushi Kaneda, Siti Nurul Zhahara, Yingbo Gong, Motoi Nishimura, Shoichiro Kawauchi, Eiryo Kawakami, Tomoaki Tanaka
Summary: The microRNA miR-874 acts as a potential tumor suppressor by suppressing target genes in various cancer types. This study reveals the relationship between miR-874-induced apoptosis and the mevalonate pathway, as well as its association with the tumor suppressor p53. The findings suggest that miR-874 suppresses the mevalonate pathway by targeting SREBF2 and PMVK, leading to the activation of the p53 pathway and promoting cell cycle arrest or apoptosis.
SCIENTIFIC REPORTS
(2022)
Review
Oncology
Raphaelly Venzel, Maria Clara Paulino Campos, Larissa Pessoa de Oliveira, Rodrigo Vasquez Dan Lins, Adamo Davi Diogenes Siena, Kim Tavares Mesquita, Talita Pollyana Moreira dos Santos, Nijiro Nohata, Lucas Coelho Marliere Arruda, Helioswilton Sales-Campos, Marinaldo Pacifico Cavalcanti Neto
Summary: Traditional therapeutic approaches for malignant melanoma have limitations and ineffectiveness in improving patient survival and tumor recurrence. Immunotherapy, as a promising alternative, modulates cell signaling pathways involved in the immune system's effector mechanisms, known as immunological checkpoints, to boost antitumor responses. Clinical studies on immunotherapeutic regimens have shown encouraging results in recent decades. This review discusses current immunotherapeutic regimens, molecular and cellular mechanisms, and clinical studies of immunotherapy in melanoma.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2023)
Article
Genetics & Heredity
Yoshiaki Shinden, Tadahiro Hirashima, Nijiro Nohata, Hiroko Toda, Reona Okada, Shunichi Asai, Takako Tanaka, Yuto Hozaka, Takao Ohtsuka, Yuko Kijima, Naohiko Seki
Summary: Our recent research identified certain passenger strands of miRNAs that function as tumor-suppressive miRNAs in cancer cells, and analyzed the miRNA expression signature of breast cancer (BrCa) to identify oncogenic genes controlled by pre-miR-99a. The study revealed FAM64A as a potential target and highlighted the importance of aberrant expression of FAM64A in the malignant transformation of BrCa.
JOURNAL OF HUMAN GENETICS
(2021)
