Article
Biochemistry & Molecular Biology
Zhi-hao Su, Jun-li Lv, Qi Ou, Zi-qin Zhao, Kai-yi Zheng, Xiao-ying Zhang, Wen-qing Lai, Xue-yang Wang, Ming-jie Deng, Mu-wang Li
Summary: The white epidermis of silkworms is caused by uric acid crystals accumulation. Abnormal metabolism of uric acid reduces its production, resulting in a transparent or translucent phenotype. This study examined the metabolite changes following Bombyx mori nucleopolyhedrovirus (BmNPV) infection in two strains of silkworms (p50 and op50) and found that the uric acid pathway plays a critical role in resistance. Feeding with inosine, a metabolite in this pathway, significantly enhanced larval resistance and modulated other metabolic pathways. The results provide insights into the resistance mechanism of silkworms to BmNPV and offer new strategies for pest control.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Jing Xu, Xiaole Xie, Qiuqin Ma, Lu Zhang, Yu Li, Yin Chen, Kang Li, Yang Xiao, Gianluca Tettamanti, Hanhong Xu, Ling Tian
Summary: This study characterized the molecules involved in Bombyx mori nucleopolyhedrovirus (BmNPV)-silkworm interaction by RNA sequencing. The upregulated genes were mainly involved in translation, signal transduction, folding, sorting, and degradation, while the downregulated genes were predominantly related to metabolism. Knockout of specific genes inhibited or promoted BmNPV proliferation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Entomology
Xiong Wang, Guangyu Ma, Feifei Ren, Mian Muhammad Awais, Jingchen Sun
Summary: Research has shown that Bombyx mori fatty acid binding protein 1 (BmFABP1) is expressed in silkmoth cells and in the tissues of silkmoth larvae, where it inhibits the replication of Bombyx mori nucleopolyhedrovirus (BmNPV). This study provides new insights into the potential antiviral role of BmFABP1 and its importance in breeding BmNPV-resistant transgenic silkmoths.
Article
Microbiology
Yanting Liang, Weifan Xu, Yanyan Zhou, Yun Gao, Huan Tian, Xiaofeng Wu, Yusong Xu, Huabing Wang
Summary: The protein BmSUH plays a crucial role in the oral infection of BmNPV by mediating the entry of ODVs into the midgut. This study provides insights into preventing BmNPV infection through disrupting essential protein-protein interactions.
Article
Virology
Mudasir Gani, Sergei Senger, Satish Lokanath, Pawan Saini, Kamlesh Bali, Rakesh Gupta, Vankadara Sivaprasad, Johannes A. Jehle, Joerg T. Wennmann
Summary: The study revealed the distribution of Indian BmNPV and investigated their genetic composition through methods like gene sequencing and phylogenetic analysis. It found genetic variations among different isolates and identified some new closely related viral strains.
Article
Virology
Guanping Chen, Yuedong Li, Xiangshuo Kong, Shudi Zhao, Jiale Li, Xiaofeng Wu
Summary: This study found that the expression of Bombyx mori HEXIM1 (BmHEXIM1) was significantly upregulated after BmNPV infection. By suppressing BmRelish-driven immune responses, BmHEXIM1 assisted in the immune escape of BmNPV. This research provides new insights into the mechanisms of immune escape of BmNPV and theoretical guidance for the breeding of BmNPV-resistant silkworm varieties.
Article
Entomology
Jun-Qing Ge, Zhu-Hong Wang, Xi Chen, Hua Chen, Jian Huang
Summary: BmNPV p26 is conserved among all completely sequenced Lepidoptera baculoviruses and may play an important role in the virus infection cycle, with functions in both the cytoplasm and nucleus. RNAi-based knockdown of p26 decreased budded virus production in BmNPV-infected cells and may be associated with BmNPV replication during the late infection stage.
Article
Entomology
Xiong Wang, Guangyu Ma, Feifei Ren, Mian Muhammad Awais, Jingchen Sun
Summary: This study investigates the interactions between the Bombyx mori nucleopolyhedrovirus (BmNPV) and its host, identifying potential proteins involved in BmNPV invasion and proliferation. The results provide valuable information for understanding the replication process of BmNPV and identifying potential receptor proteins.
Article
Fisheries
Xiaoli Kang, Yaping Wang, Wenjuan Liang, Xin Tang, Yan Zhang, Lingyan Wang, Ping Zhao, Zhongyan Lu
Summary: BmFoxO expression decreases after BmNPV infection in silkworms, affecting PEPCK expression. Overexpression of BmFoxO enhances antiviral resistance in silkworms by upregulating PEPCK.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Virology
Miao Hu, Yi You, Yao Li, Shiyi Ma, Jiaqi Li, Meng Miao, Yanping Quan, Wei Yu
Summary: A recent study found that the nucleopolyhedrovirus BmNPV modifies the mitochondrial ACO2 enzyme through acetylation at lysine 56. Deacetylation of ACO2 decreases its enzymatic activity and impairs ATP production and mitochondrial function. Lowering ACO2 acetylation also reduces BmNPV replication and the generation of progeny viruses.
Article
Biochemistry & Molecular Biology
Qian Wang, Miao Cui, Cong Zhang, Anqi Xia, Qiang Wang, Xiaoyong Liu, Keping Chen, Hengchuan Xia
Summary: The cloning of the SOCS7 gene in the silkworm and its role in the immune response against infection were investigated in this study.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xiong Wang, Yinong Zhang, Shigang Fei, Mian Muhammad Awais, Hao Zheng, Min Feng, Jingchen Sun
Summary: The study revealed that the Heat Shock Protein 75 (TRAP1) in Bombyx mori may play a crucial role in the proliferation of Bombyx mori nucleopolyhedrovirus, with the expression levels of TRAP1 affecting the expression of the viral capsid protein.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Xianghan Mei, Chun Li, Peilin Peng, Jue Wang, Enxi He, Zhiyong Qiu, Dingguo Xia, Qiaoling Zhao, Dongxu Shen
Summary: In this study, a C-type lectin gene called BmIML-2 was identified from the silkworm Bombyx mori. It was found that BmIML-2 can inhibit the proliferation of the BmNPV virus by facilitating cell apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zhi-Gang Hu, Zhan-Qi Dong, Jiang-Hao Miao, Ke-Jie Li, Jie Wang, Peng Chen, Cheng Lu, Min-Hui Pan
Summary: The key functional domains of Bombyx mori nucleopolyhedrovirus (BmNPV) immediate early protein 1 (IE1) have been identified through sequence analyses and truncation experiments. The C-terminus of IE1 is more conserved than the N-terminus, and specific regions within IE1 are necessary for its localization and transcriptional activation activity. These findings contribute to the understanding of the mechanism of baculovirus infection and provide potential targets for the development of small molecule IE1 mutants as agonists or antagonists.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Xiu Shi, Yaxin Zhang, Tianchen Zhu, Nan Li, Sufei Sun, Min Zhu, Jun Pan, Zeen Shen, Xiaolong Hu, Xing Zhang, Chengliang Gong
Summary: The study utilized 16S rDNA sequencing and metabolomics to show significant alterations in gut microbiota and metabolites of silkworm midgut after BmNPV infection. Analysis revealed enrichment of specific pathways related to viral infection and metabolism, highlighting the relationship between digestive tract gut microbiota, metabolites, and host-virus interaction. These findings enhance understanding of viral infection links to midgut microbiota and metabolic activity in the silkworm model.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yinu Li, Feng Jiang, Xiaofeng Shi, Xingjian Liu, Huipeng Yang, Zhifang Zhang
DNA AND CELL BIOLOGY
(2016)
Article
Immunology
Xuexing Zheng, Shukun Wang, Weijiao Zhang, Xingjian Liu, Yongzhu Yi, Songtao Yang, Xianzhu Xia, Yinv Li, Zhifang Zhang
INTERNATIONAL IMMUNOPHARMACOLOGY
(2016)
Article
Multidisciplinary Sciences
Xingjian Liu, Yonglong Wei, Yinu Li, Haoyang Li, Xin Yang, Yongzhu Yi, Zhifang Zhang
Article
Genetics & Heredity
Mehboob Arslan, Xin Yang, Diwakar Santhakumar, Xingjian Liu, Xiaoyuan Hu, Muhammad Munir, Yinu Li, Zhifang Zhang
Article
Virology
Lulu Zhao, Xingjian Liu, Kai Tang, Zhifang Zhang, Huan Zhang, Yinu Li
Summary: This study characterized the biological and genetic features of HearNPV-BJ, a strain of Helicoverpa assulta nucleopolyhedrovirus. The whole genome sequence analysis revealed its high similarity with other Helicoverpa armigera nucleopolyhedroviruses and suggested that HearNPV-BJ may be a variant of this species.
Article
Pharmacology & Pharmacy
Dan Li, Haozhi Song, Jialei Li, Xingjian Liu, Xintao Gao, Tong Wu, Zhifang Zhang, Yinu Li
Summary: This study proposed a novel nanoparticle vaccine strategy using ferritin self-assembly technology for Peste des Petits Ruminants Virus (PPRV). The fusion of PPRV hemagglutinin (H) protein with ferritin significantly increased the expression level, immunogenicity, and protective immune response compared to the antigen alone. The use of ferritin nanoparticles as antigen delivery carriers showed promise in enhancing antigen expression and improving immunogenicity and immune response.