期刊
ONCOTARGET
卷 8, 期 9, 页码 14443-14461出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.14804
关键词
cancer; endosome; extracellular vesicle; mesenchymal stromal cell; nuclear envelope invagination
资金
- Roseman University
- Sachsisches Staatsministerium fur Wissenschaft und Kunst [4-7531.60/29/31]
- Deutsche Forschungsgemeinschaft [SFB655-B3]
Extracellular membrane vesicles (EVs) function as vehicles of intercellular communication, but how the biomaterials they carry reach the target site in recipient cells is an open question. We report that subdomains of Rab7(+) late endosomes and nuclear envelope invaginations come together to create a sub-nuclear compartment, where biomaterials associated with CD9(+) EVs are delivered. EV-derived biomaterials were also found in the nuclei of host cells. The inhibition of nuclear import and export pathways abrogated the nuclear localization of EV-derived biomaterials or led to their accumulation therein, respectively, suggesting that their translocation is dependent on nuclear pores. Nuclear envelope invagination-associated late endosomes were observed in ex vivo biopsies in both breast carcinoma and associated stromal cells. The transcriptome of stromal cells exposed to cancer cell-derived CD9(+) EVs revealed that the regulation of eleven genes, notably those involved in inflammation, relies on the nuclear translocation of EV-derived biomaterials. Our findings uncover a new cellular pathway used by EVs to reach nuclear compartment.
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