Review
Oncology
Mateusz Smolarz, Piotr Widlak
Summary: Exosomes are emerging as a potential source of cancer biomarkers, particularly through the miRNA content. Despite promising research results, no biomarkers for early cancer detection have been clinically validated yet. This highlights the constant need for new biomarker candidates in the field of lung cancer research.
Article
Veterinary Sciences
Matias Aguilera-Rojas, Soroush Sharbati, Torsten Stein, Mario Candela Andrade, Barbara Kohn, Ralf Einspanier
Summary: This study investigated the effects of haemolysis on circulating miRNAs in dogs, and found that some miRNAs were significantly sensitive to haemolysis. The study also revealed differences in canine miRNA abundance compared to human miRNA. These findings have important implications for the discovery of circulating biomarkers in dogs, and emphasize the need to carefully consider the impact of haemolysis in serum or plasma-based tests.
BMC VETERINARY RESEARCH
(2022)
Article
Immunology
Emilio Nunez-Borque, Sergio Fernandez-Bravo, Pablo Rodriguez Del Rio, Lucia Palacio-Garcia, Angela Di Giannatale, Virginia Di Paolo, Angela Galardi, Marta Colletti, Luisa Pascucci, Jaime Tome-Amat, Javier Cuesta-Herranz, Maria Dolores Ibanez-Sandin, Jose Julio Laguna, Alberto Benito-Martin, Vanesa Esteban
Summary: This study investigates the involvement of miRNAs in anaphylactic reactions and their potential as biomarkers. The researchers found differential expression profiles of circulating miRNAs in patients with anaphylaxis, particularly miR-375-3p. Functional and molecular experiments demonstrated that miR-375-3p plays a negative role in the stability of the endothelial barrier. These findings provide insights into the molecular mechanisms underlying anaphylactic reactions and suggest the potential of miR-375-3p as a biomarker.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Jolanta Kryczka, Monika Migdalska-Sek, Jacek Kordiak, Justyna M. Kiszalkiewicz, Dorota Pastuszak-Lewandoska, Adam Antczak, Ewa Brzezianska-Lasota
Summary: The study aimed to find diagnostic and/or prognostic biomarkers in non-small cell lung cancer (NSCLC) patients based on circulating microRNAs in extracellular vesicles (EVs). Results showed significantly lower levels of miR-23a and miR-let7i in NSCLC patients compared to controls, with potential diagnostic value. ROC curve analysis demonstrated the potential of miR-23a and miR-let7i as non-invasive markers for NSCLC diagnosis.
Article
Cell Biology
Joanna Patrycja Wroblewska, Michal Stefan Lach, Marcin Rucinski, Igor Piotrowski, Lukasz Galus, Wiktoria Maria Suchorska, Stephanie Kreis, Andrzej Marszalek
Summary: The study found that miRNAs extracted from exosomes of UM patients could be potential biomarkers for diagnosis and metastatic spread of UM.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Cuong Viet Pham, Snehal Midge, Hridika Barua, Yumei Zhang, Tuong Ngoc-Gia Nguyen, Roberto A. Barrero, Andrew Duan, Wang Yin, Guoqin Jiang, Yingchun Hou, Shufeng Zhou, Yiming Wang, Xiaoqing Xie, Phuong H. L. Tran, Dongxi Xiang, Wei Duan
Summary: The use of 'exosome depleted serum' in cell culture-based studies may introduce artifacts into research examining the function of human and rodent EVs, especially those involving EV miRNA. Researchers are urged to seriously reconsider the practice of using 'exosome depleted serum' in the production of human and other mammalian EVs in vitro.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Gastroenterology & Hepatology
Qichen Shen, Zhuizui Huang, Lingyan Ma, Jiachen Yao, Ting Luo, Yao Zhao, Yingping Xiao, Yuanxiang Jin
Summary: Inflammatory bowel disease (IBD) is a global disease without a cure, and disruption of the microbial ecosystem is considered a major cause. This study investigated the role of extracellular vesicles (EVs) and membrane vesicles (MVs) in host-microbe interactions in colitis. The researchers found that miR-181b-5p transplantation inhibited inflammation and promoted macrophage polarization, while miR-200b-3p regulated the microbiota composition and contributed to intestinal barrier integrity. MVs from feces and BMVs from colitis also had similar effects. These findings provide new insights and potential treatment strategies for colitis.
Article
Virology
Steven Kopcho, Marina McDew-White, Wasifa Naushad, Mahesh Mohan, Chioma M. Okeoma
Summary: This study comprehensively analyzed the composition of circulating exmiRNAs and their association with extracellular particles, such as EVs and ECs, using sRNA sequencing. The study also found that SIV infection can alter the miRNA profile of EVs and suggested that miR-128-3p may be a potential target of HIV/SIV. This research has important implications for the development of biomarker approaches for various diseases based on the analysis of circulating exmiRNAs.
Article
Biochemistry & Molecular Biology
Onur Tokgun, Kubilay Inci
Summary: The study demonstrates the association between MYC gene expression, small extracellular vesicles (sEVs) function, and miRNA composition in SCLC. The findings suggest MYC and sEVs as potential therapeutic targets for SCLC treatment.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Oncology
Jolene Chisholm, Sandor Haas-Neill, Peter Margetts, Khalid Al-Nedawi
Summary: Prostate cancer (PC) is a major cause of death in men, and current biomarker tests have limitations in accurately diagnosing the disease. This study examined the protein, mRNA, and miRNA content of extracellular vesicles (EVs) collected from PC patients and healthy subjects. The analysis revealed distinct groups of proteins, mRNAs, and miRNAs that differed between healthy subjects and PC patients, as well as between different tumor types. These findings suggest potential molecular markers for diagnosing PC.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ari Ogaki, Yuji Ikegaya, Ryuta Koyama
Summary: Extracellular vesicles (EVs) composed of lipid bilayer membranes and containing various molecules like mRNA and miRNA play a crucial role in intercellular communication in the brain. Astrocytes, as the most abundant glial cells in the mammalian brain, may be influenced by EVs from inside and outside the brain, subsequently affecting brain function. Further research on the specific targeting mechanism of EVs to astrocytes could open up new therapeutic possibilities for brain diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Chaolin Deng, Min-Er Zhong, Yanyu Chen, Meng Pan, Lai Xu, Yi Xiao, Yanpan Gao, Bin Wu
Summary: This study analyzed the differential protein profiles of serum-derived extracellular vesicles (EVs) between mucinous adenocarcinoma (MC) and non-mucinous adenocarcinoma (AC), and identified the key EV protein PLA2G2A associated with MC pathogenesis. Overexpression of PLA2G2A in colon cancer cell line promoted cell invasion and migration ability. PLA2G2A may serve as a prognostic predictive marker for colon cancer patients with BRAF mutations.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Oncology
Rosario Distefano, Giovanni Nigita, Patricia Le, Giulia Romano, Mario Acunzo, Patrick Nana-Sinkam
Summary: Post-transcriptional modifications in miRNA molecules have provided new insights into cancer biology and potential cancer biomarkers. In this study, we analyzed the profiles of canonical and non-canonical miRNAs in lung cancer patients of different races. We identified distinct post-transcriptional modifications in lung cancer tissues, indicating the relevance of miRNA isoforms as potential biomarkers. Concurrent profiling of canonical and non-canonical miRNAs may help identify additional biological pathways and biomarkers in lung cancer.
Article
Biochemistry & Molecular Biology
Pina Fusco, Anna Fietta, Maria Rosaria Esposito, Luca Zanella, Sara Micheli, Angelica Bastianello, Lorenzo Bova, Giulia Borile, Giuseppe Germano, Elisa Cimetta
Summary: Tumor hypoxia stimulates release of extracellular vesicles (EVs) that facilitate short- and long-range intercellular communication and metastatization. The role of hypoxic EVs and their miR-210-3p cargo enrichment in promoting Neuroblastoma (NB) dissemination was investigated. The study found that hypoxic EVs were more potent in inducing NB cells migration and colony formation, and miR-210-3p was identified as the most abundant miRNA in the cargo of hypoxic EVs. Overexpression of miR-210-3p in normoxic EVs conferred them pro-metastatic features, while miR-210-3p silencing suppressed the metastatic ability of hypoxic EVs both in vitro and in vivo.
CELL AND BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Cinzia Giordano, Felice Maria Accattatis, Luca Gelsomino, Piercarlo Del Console, Balazs Gyorffy, Mario Giuliano, Bianca Maria Veneziani, Grazia Arpino, Carmine De Angelis, Pietro De Placido, Erica Pietroluongo, Francesco Zinno, Daniela Bonofiglio, Sebastiano Ando, Ines Barone, Stefania Catalano
Summary: This study identified novel miRNA signatures, miRNA-27a and miRNA-128, in breast cancer by analyzing the expression profiles of EV-derived miRNAs in the blood circulation. These miRNAs showed diagnostic accuracy in distinguishing breast cancer patients from non-cancer controls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Developmental Biology
James Jabalee, Tamara A. Franz-Odendaal
DEVELOPMENTAL BIOLOGY
(2015)
Article
Oncology
Christopher T. D. Dickman, James Lawson, James Jabalee, Sara A. MacLellan, Nancy E. LePard, Kevin L. Bennewith, Cathie Garnis
Article
Oncology
James Jabalee, Anita Carraro, Tony Ng, Eitan Prisman, Cathie Garnis, Martial Guillaud
ANALYTICAL CELLULAR PATHOLOGY
(2018)
Article
Anatomy & Morphology
J. Jabalee, S. Hillier, T. A. Franz-Odendaal
JOURNAL OF ANATOMY
(2013)
Review
Cell Biology
James Jabalee, Rebecca Towle, Cathie Garnis
Article
Biochemistry & Molecular Biology
James Lawson, Christopher Dickman, Rebecca Towle, James Jabalee, Ariana Javer, Cathie Garnis
MOLECULAR CARCINOGENESIS
(2019)
Review
Biochemistry & Molecular Biology
Issraa Shoucair, Fernanda Weber Mello, James Jabalee, Saeideh Maleki, Cathie Garnis
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Saeideh Maleki, James Jabalee, Cathie Garnis
Summary: Drug resistance remains a major obstacle in cancer treatment, with various mechanisms contributing to its development. Extracellular vesicles (EVs) derived from cancer cells play critical roles in tumor progression, metastasis, and drug resistance by delivering protein and genetic material to cells of the tumor microenvironment. Understanding the biochemical and genetic mechanisms underlying drug resistance will aid in the development of new therapeutic strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)