Article
Biology
Ju Wang, Wei Zheng, Shuoping Zhang, Keqiang Yan, Miao Jin, Huiling Hu, Zhen Ma, Fei Gong, Guangxiu Lu, Yan Ren, Liang Lin, Ge Lin, Liang Hu, Siqi Liu
Summary: This study established a model for evaluating embryo or oocyte quality based on metabolites in follicular fluid, with phosphatidylcholines identified as key contributors. The increase of PCs in follicular fluid may lead to a decrease in embryo quality. Pathway analysis suggested that accumulation of arachidonic acid induced by PCs could weaken embryo quality.
Review
Biochemistry & Molecular Biology
Ana Teresa Brinca, Ana Cristina Ramalhinho, Angela Sousa, Antonio Helio Oliani, Luiza Breitenfeld, Luis A. Passarinha, Eugenia Gallardo
Summary: PCOS is one of the main causes of anovulatory infertility and its etiology and pathophysiology are still unclear. PCOS patients undergoing assisted reproductive techniques may experience poor response, low oocyte quality, and ovarian hyperstimulation syndrome. Metabolomic analysis can improve the success rate of assisted reproductive technology.
Article
Agriculture, Dairy & Animal Science
Huaxing Zhao, Xiaohua He, Xianjun Zhang, Junsong Shi, Rong Zhou, Ranbiao Mai, Qiaoyun Su, Gengyuan Cai, Sixiu Huang, Zheng Xu, Zhenfang Wu, Zicong Li
Summary: The quality of in vitro matured oocytes is usually lower than that of in vivo matured oocytes, possibly due to the absence of some important signal regulators in vitro. In this study, we found that progesterone and androstenedione were upregulated in the follicular fluid (FF) during in vivo pig oocyte maturation. The supplementation of progesterone or androstenedione during in vitro maturation significantly improved the pig oocyte maturation rate and subsequent embryo developmental competence.
Article
Biochemistry & Molecular Biology
Di Zhang, Jia Lv, Rongxin Tang, Ying Feng, Yongfeng Zhao, Xiaoyang Fei, Richeng Chian, Qigui Xie
Summary: The study investigated exosomal microRNAs in human ovarian follicular fluid to explore their potential association with oocyte quality. Analysis revealed that several exosomal miRNAs were critical for maintaining oocyte quality, suggesting they may be potential biomarkers for evaluating oocyte quality, which is important in clinical settings.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Obstetrics & Gynecology
Berna Aslan Cetin, Pelin Ocal, Tulay Irez, Ezel Uslu, Kubra Irmak, Suat Karatas
Summary: The study found a correlation between sialic acid levels in follicular fluid and oocyte quality during in vitro fertilization (IVF), but not with clinical parameters. Sialic acid levels in the follicular fluid may play a role in normal oocyte maturation or act as a compensatory mechanism against reactive oxygen species during the maturation process.
REPRODUCTIVE SCIENCES
(2022)
Article
Endocrinology & Metabolism
Ziyu Guo, Jihong Yang, Guangping Yang, Ting Feng, Xinyue Zhang, Yao Chen, Ruizhi Feng, Yun Qian
Summary: The study found that higher levels of NAM in large follicles are associated with larger follicle development and higher rates of oocyte maturation and fertilization. Adding 5mM NAM during in vitro maturation process can improve mice oocyte quality and reduce damage caused by oxidative stress.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Genetics & Heredity
Yue Liu, Zhiheng Yu, Shigang Zhao, Lei Cheng, Yuanyuan Man, Xueying Gao, Han Zhao
Summary: Elevated oxidative stress levels were found in both the serum and follicular fluid of PCOS patients compared to normal controls, with the former showing more severe oxidative stress. The study revealed that oxidative stress in follicular fluid has a stronger impact on embryo quality, highlighting the importance of this factor in future research.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Obstetrics & Gynecology
Robert B. Hood, Donghai Liang, Youran Tan, Jennifer B. Ford, Irene Souter, Jorge E. Chavarro, Dean P. Jones, Russ Hauser, Audrey J. Gaskins
Summary: This study identified the metabolic pathways and metabolites in the serum and follicular fluid that are associated with peak estradiol levels and the number of mature oocytes. The serum metabolome mainly involves fatty acid and amino acid pathways, while the follicular fluid metabolome mainly involves lipid, vitamin, and hormone pathways. These findings provide insights into the biological mechanisms underlying follicular and oocyte development.
HUMAN REPRODUCTION
(2023)
Article
Endocrinology & Metabolism
Ying Ding, Yihong Jiang, Mingjiang Zhu, Qinling Zhu, Yaqiong He, Yao Lu, Yuan Wang, Jia Qi, Yifan Feng, Rong Huang, Huiyong Yin, Shengxian Li, Yun Sun
Summary: This study investigated the lipidomic profiles in follicular fluid (FF) of different phenotypes of Polycystic ovary syndrome (PCOS) and identified metabolic signatures and candidate biomarkers that provide a better understanding of the pathogenesis of PCOS.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anne-Sophie Neyroud, Regina Maria Chiechio, Gregory Moulin, Solene Ducarre, Claire Heichette, Aurelien Dupont, Mathieu Budzynski, Pascale Even-Hernandez, Maria Jose Lo Faro, Marina Yefimova, Valerie Marchi, Celia Ravel
Summary: The morphology and quantification of extracellular vesicles (EVs) in follicular fluid (FF) of healthy individuals were studied in this research. EVs play important roles in the development of female gametes, but limited knowledge exists regarding their morphology. The findings of this study provide a comprehensive morphological description and quantification of FF-derived EVs, serving as a reference for further research on EVs in ovarian diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Sweta Ravisankar, Kelsey E. Brooks, Melinda J. Murphy, Nash Redmayne, Junghyun Ryu, Jason M. Kinchen, Shawn L. Chavez, Jon D. Hennebold
Summary: The administration of gonadotropins during infertility treatment affects the development of ovarian follicles and the competency of oocytes. Metabolomics analysis identified unique metabolites in follicular fluid that were associated with different embryo classifications, with an increase in the cortisol to cortisone ratio observed in the blastocyst group. The glucocorticoid receptor NR3C1 plays a role in regulating follicular processes through paracrine signaling, but further studies are needed to understand its specific mechanisms.
SCIENTIFIC REPORTS
(2021)
Article
Endocrinology & Metabolism
Li Yu, Miao Liu, Shiji Xu, Zhenxin Wang, Te Liu, Jiaye Zhou, Doudou Zhang, Xi Dong, Baishen Pan, Beili Wang, Suying Liu, Wei Guo
Summary: This study evaluated the role of hormones and mitochondrial function in embryo development during in vitro fertilization cycles. The results showed that LH and FSH levels in follicular fluid could predict the formation of high-quality embryos, and embryo development was associated with mitochondrial function in the follicular fluid.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Aleksandra E. Lewandowska, Anna Fel, Marcel Thiel, Paulina Czaplewska, Krzysztof Lukaszuk, Jacek R. Wisniewski, Stanislaw Oldziej
Summary: Two label-free quantitative workflows based on different high-resolution mass spectrometers and LC setups were presented for the analysis of human follicular fluid proteome, with around 1000 proteins identified in each workflow. The concentration values obtained from both workflows showed significant correlation, leading to unified lists of proteins linked to oocyte maturity and blastocyst development. The Quad-Orbitrap workflow is suitable for in-depth analysis of low abundant proteome without extensive fractionation, while the Triple Quad-TOF workflow offers a more robust approach with potential for effectiveness with increasing number of analyzed samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Alice Bongrani, Ingrid Plotton, Namya Mellouk, Christelle Rame, Fabrice Guerif, Pascal Froment, Joelle Dupont
Summary: This study aimed to characterize the expression profile of androgens in the follicular fluid of PCOS patients and compare it to control women and women with only polycystic ovary morphology. The results showed that the intrafollicular concentrations of androgens synthesized by ovarian theca cells were significantly higher in PCOS patients compared to control women, and were positively correlated with PCOS clinical and biological features. Additionally, there was a significant correlation between the intrafollicular concentrations of androgens and adipokines, suggesting a close relationship between these two hormonal systems in ovarian physiology and PCOS physiopathology.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Genetics & Heredity
Aalaap Naigaonkar, Roshan Dadachanji, Indira Hinduja, Srabani Mukherjee
Summary: Women with PCOS undergoing IVF have disrupted redox balance in follicular fluid, potentially impacting oocyte/embryo quality. Granulosa cells may play a crucial role in maintaining follicular redox balance. The glutathione system and paraoxonase1 could be further explored as surrogate markers for IVF prognosis/outcome.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)