期刊
ONCOTARGET
卷 8, 期 26, 页码 43192-43200出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.17860
关键词
satellite cells; differentiation capacity; mTOR signaling pathway
资金
- Science and Technology Planning Projects of Guangdong Province, China [2016A050502045, 6101821101032017]
- Science and Technology Planning Project of Guangzhou, China [201704030005]
- Natural Science Foundation of Guangdong Province, China [2016A030313417]
We isolated and cultured satellite cells (SCs) from the longissimus dorsi muscles of 1- day- old male Landrace and Lantang piglets to compare the SC differentiation capacity in the two breeds. Lantang piglets yielded more (P < 0.05) SCs per gram of muscle than Landrace piglets (5.2 +/- 0.9x10(4) vs. 2.4 +/- 0.2x10(4)). Transcription of the differentiation markers myogenin and myosin heavy chain I (MyHC I) in the longissimus dorsi muscle was higher in Lantang than Landrace piglets (P < 0.05). Protein levels of myogenin (P < 0.05), MyHC I (P < 0.05), and myogenic regulatory factor 4 (P = 0.07) were higher in Lantang than Landrace piglet SCs after 72 h of differentiation. Creatine kinase activity was higher in Lantang than Landrace piglet SCs after 24, 48, and 72 h of differentiation (P < 0.05), and there was a greater fusion index in Landrace piglet SCs after 72 h of differentiation. In addition, phosphorylation of Akt, mTOR, S6K1, S6, and 4EBP1 was lower in Lantang than Landrace piglet SCs (P < 0.05). Thus differentiation was more extensive in Lantang than Landrace piglet SCs, but expression of the mTOR signaling pathway was lower in Lantang piglet SCs, suggesting mTOR signaling may inhibit myogenic differentiation. These findings reveal that mTOR signaling is a factor in myogenesis and imply that mTOR could potentially serve as an activator of myoblast differentiation and muscle regeneration.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据