Article
Cell Biology
Changhao Wu, Chenglong Zheng, Shiyu Chen, Zhiwei He, Hao Hua, Chengyi Sun, Chao Yu
Summary: Pancreatic cancer (PC) is a gastrointestinal tract malignant tumor with a poor prognosis. FOXQ1 has been found to be overexpressed in PC tissues and associated with poor prognosis. FOXQ1 overexpression promotes PC cell proliferation, tumor stemness, invasion, and metastasis, while FOXQ1 silencing shows the reverse effect. Mechanistic studies reveal that FOXQ1 promotes LDHA transcription to enhance aerobic glycolysis, contributing to the progression of PC.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Lucia Simona Ferraraccio, Paolo Bertoncello
Summary: In this study, dehydrogenase enzymes for glucose and lactate were encapsulated in alginate hydrogels and deposited on glassy carbon electrodes. These enzyme modified alginate hydrogels were used as electrochemiluminescence (ECL)-based biosensors for the detection of glucose and lactate. The ECL response was obtained through the redox reaction between the substrate, NAD+, and the encapsulated enzyme. The results suggest that the encapsulated dehydrogenases have potential for the development of biocompatible sensors for glucose and lactate detection in physiological fluids.
BIOELECTROCHEMISTRY
(2023)
Review
Oncology
Eva Kocianova, Viktoria Piatrikova, Tereza Golias
Summary: This review discusses the role and significance of lactate in cancer, as well as recent findings on lactate metabolism and signaling pathways in cancer.
Article
Cell Biology
Leonie K. Stabenow, Darya Zibrova, Claudia Ender, Dario L. Helbing, Katrin Spengler, Christian Marx, Zhao-Qi Wang, Regine Heller
Summary: Vascular aging is associated with endothelial dysfunction, which is influenced by the accumulation of senescent cells in aged tissues and their environment. Senescence is closely linked to changes in cell metabolism, with senescent cells showing enhanced glycolytic and oxidative glucose metabolism.
Article
Biochemistry & Molecular Biology
Anastasya Boyarski, Noam Shlush, Shiraz Paz, Jerry Eichler, Lital Alfonta
Summary: In this study, four different enzymatic constructs of L-lactate dehydrogenase were compared and the fusion enzyme containing two cytochromes showed superior catalytic and electrochemical properties. This fusion enzyme exhibited higher activity, lower redox potential, wider dynamic range, and higher product inhibition concentration than the native enzyme. Additionally, it displayed measurable lactate-dependent oxygen reduction.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Kyuseok Lee, Gyeoungsun Song, Jungwook Kwon, Jihyeon Kim, Haesik Yang
Summary: In this study, a zwitterionic phenazine compound, mPPS, was used as an organic catalyst and electron mediator for the oxidation of NADH and the detection of glucose and LDH. mPPS exhibited high stability in phosphate buffered saline and its reduced form displayed good water solubility.
Article
Medicine, General & Internal
Murat Yilmaz, Beliz Oztok Tekten
Summary: This study aimed to determine the potential role of postictal serum LDH activities in differentiating epileptic seizures from non-epileptic seizures. The results showed significantly higher LDH activities in patients with epileptic seizures compared to those with non-epileptic seizures, suggesting the potential use of LDH activities as a diagnostic tool in distinguishing between seizure types.
Article
Cell Biology
Xiukun Hou, Xianle Shi, Wei Zhang, Dapeng Li, Linfei Hu, Jihong Yang, Jingzhu Zhao, Songfeng Wei, Xi Wei, Xianhui Ruan, Xiangqian Zheng, Ming Gao
Summary: High expression levels of LDHA in papillary thyroid carcinoma are associated with aggressive clinicopathological features and poor prognosis. LDHA promotes cancer cell migration, invasion, and proliferation, making it a potential therapeutic target.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Tomomi Minemura, Atsunori Fukuhara, Michio Otsuki, Iichiro Shimomura
Summary: The study reveals that LDHA regulates Glut1 expression and basal glucose uptake in adipocytes. Knockout or inhibition of LDHA leads to decreased Glut1 protein levels and reduced glucose uptake.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Maitham A. Khajah, Sarah Khushaish, Yunus A. Luqmani
Summary: This study revealed that the expression, activity, and lactate production of LDH-A and B were significantly enhanced in endocrine-resistant breast cancer cells. Targeting LDH might be an effective strategy to inhibit cancer cell motility.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Medicine, Research & Experimental
John M. Macharia, Zsolt Kaposztas, Timea Varjas, Ferenc Budan, Afshin Zand, Imre Bodnar, Raposa L. Bence
Summary: Although the pathophysiology of colorectal cancer (CRC) is complex and not well understood, interactions between risk factors play a crucial role in the development and progression of the disease. The use of lactic acid bacteria (LAB) prebiotics and probiotics to modulate the tumor microenvironment (TME) has become increasingly popular in CRC management. This study aims to investigate the negative effects of LAB-derived lactic acid in the colonic mucosa in colorectal cancer management. More research is needed to ensure that LAB and their lactic acid by-products do not have unintended adverse effects in CRC management.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Cell Biology
Chuanyu Guo, Monika Deshpande, Yueqi Niu, Isha Kachwala, Miguel Flores-Bellver, Haley Megarity, Taylor Nuse, Savalan Babapoor-Farrokhran, Michael Ramada, Jaron Sanchez, Neelay Inamdar, Thomas V. Johnson, Maria Valeria Canto-Soler, Silvia Montaner, Akrit Sodhi
Summary: Tight glycemic control is essential for managing diabetes and preventing microvascular disease. However, this control can lead to episodes of hypoglycemia, which have adverse effects. This study shows that low glucose levels induce the expression of Glut1, a glucose transporter, in retinal cells through hypoxia-inducible factor (HIF)-1. This response to low glucose levels results in increased secretion of vasoactive mediators that promote diabetic retinopathy. These findings have implications for optimizing glucose management in diabetic patients.
Article
Biochemistry & Molecular Biology
Michele Mannelli, Tania Gamberi, Francesca Magherini, Tania Fiaschi
Summary: This study demonstrates that cachexia induction in myotubes is associated with a metabolic shift towards fermentation, and inhibiting lactate formation can prevent cachexia. The findings suggest lactate dehydrogenase as a possible new tool for counteracting the onset of this pathology.
Article
Biochemistry & Molecular Biology
Marika A. Franczak, Oliwia Krol, Gabriela Harasim, Agata Jedrzejewska, Nadia Zaffaroni, Carlotta Granchi, Filippo Minutolo, Amir Avan, Elisa Giovannetti, Ryszard T. Smolenski, Godefridus J. Peters
Summary: Malignant mesothelioma (MM) is a highly aggressive and resistant tumor. In this study, the cytotoxicity of new inhibitors of glucose transporter type 1 (GLUT-1) and lactate dehydrogenase-A (LDH-A) in relation to ATP/NAD+ metabolism, glycolysis and mitochondrial respiration was investigated. The inhibitors showed cytotoxicity in MM cells, associated with a decrease in ATP and NAD+, and were most effective in cells with the highest metabolic modulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Yuanyuan Zhang, Pengyu Jia, Kui Wang, Yan Zhang, Yuying Lv, Pei Fan, Liufei Yang, Shuyue Zhang, Tianyue Wang, Jing Zhao, Haixia Lv, Xinlin Chen, Yong Liu, Haidong Wei, Pengbo Zhang
Summary: Metabolic adaption drives microglial inflammatory responses, and lactate shapes immunological and inflammatory states. In this study, the researchers investigated the involvement of lactate in regulating microglial inflammatory responses after cerebral ischemia. They found that lactate treatment inhibited the increase in inflammatory markers and NF-kappa B pathway induced by oxygen-glucose deprivation in BV2 cells. Lactate also activated HIF-1 alpha, which played a role in the protective response of microglia. Furthermore, lactate-treated BV2 cells improved the viability and reduced the apoptosis of neurons after ischemia. These findings suggest that lactate can modulate microglial inflammatory responses and promote neuronal survival.
BRAIN RESEARCH BULLETIN
(2023)
Review
Surgery
Yang Hu, Xuan Zhou, Xiaofei Tong, Xiangyu Chen, Mingzhu Wang, Xianrui Wu, Peiting Li, Fengjie Tang, Jianda Zhou, Ping Li
Summary: This study found that the use of antibiotics after breast implantation reduces the incidence of infection. Smoking, obesity, and type II diabetes are risk factors for postoperative infections.
FRONTIERS IN SURGERY
(2022)
Review
Materials Science, Biomaterials
Lalithasri Ramasubramanian, Shixian Du, Siraj Gidda, Nataliya Bahatyrevich, Dake Hao, Priyadarsini Kumar, Aijun Wang
Summary: Cardiovascular diseases remain a major cause of global mortality. Extracellular vesicles, as nanovesicles for molecular transport and intracellular communication regulation, have potential as diagnostic biomarkers for early detection of CVD and as targeted drug delivery vesicles. However, their clinical application is limited by challenges in isolation, standardization, and delivery.
Article
Nanoscience & Nanotechnology
Lalithasri Ramasubramanian, Harsha Jyothi, Leora Goldbloom-Helzner, Brandon M. Light, Priyadarsini Kumar, Randy P. Carney, Diana L. Farmer, Aijun Wang
Summary: This study validates lipid rafts as a functional biomaterial for synthesizing biomimetic nanovesicles and shows their potential in promoting neurogenesis and angiogenesis.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Editorial Material
Cell Biology
Kaitlin C. Clark, Ashley Amador, Aijun Wang
NEURAL REGENERATION RESEARCH
(2023)
Article
Engineering, Biomedical
Alexander Stahl, Dake Hao, Janos Barrera, Dominic Henn, Sien Lin, Seyedsina Moeinzadeh, Sungwoo Kim, William Maloney, Geoffrey Gurtner, Aijun Wang, Yunzhi Peter Yang
Summary: Conventional synthetic vascular grafts have high failure rates due to mismatched mechanical properties with native vessels and poor regenerative potential. In this study, we developed a new bioactive synthetic vascular graft with tailored pore sizes to promote pro-regenerative macrophage populations and improve vascular regeneration. The grafts showed similar mechanical properties to native blood vessels and maintained patency and vascular regeneration in animal models.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Dake Hao, Ruiwu Liu, Tomas Gonzalez Fernandez, Christopher Pivetti, Jordan Elizabeth Jackson, Edwin Samuel Kulubya, Hong-Jiang Jiang, Hai-Yang Ju, Wen-Liang Liu, Alyssa Panitch, Kit S. Lam, J. Kent Leach, Diana L. Farmer, Aijun Wang
Summary: This study developed a biomaterial that regulates endogenous cell adhesion to promote vascularized bone regeneration. The biomaterial improved the adhesion of mesenchymal stem cells, osteoblasts, endothelial progenitor cells, and endothelial cells, leading to enhanced bone formation and vascularization in animal models.
BIOACTIVE MATERIALS
(2023)
Review
Medicine, General & Internal
Clifford T. Pereira, Elise E. Hill, Anastasiya Stasyuk, Neil Parikh, Jannat Dhillon, Aijun Wang, Andrew Li
Summary: Peripheral nerve injuries requiring surgical repair have three accepted methods: end-to-end, end-to-side, and side-to-side neurorrhaphy, each with specific indications. Understanding the circumstances for each method is important, but having a deeper understanding of the molecular mechanisms underlying repair can aid surgeons in decision-making and guide technique nuances. This paper aims to summarize the similarities and divergences of the three commonly used nerve repair strategies and the scope of molecular mechanisms and signal transduction pathways in nerve regeneration, as well as identify knowledge gaps for improving clinical outcomes in patients.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Medicine, Research & Experimental
Clifford Pereira, Sabrina Valentina Lazar, Aijun Wang
Summary: Peripheral nerve injury (PNI) is a common neurological injury, and interpositional nerve grafts are used to bridge the nerve gaps. Rapid revascularization of the grafts is crucial for satisfactory nerve regeneration. Off-the-shelf nerve allografts or scaffolds are being investigated as alternatives to autografts due to their advantages such as a wide variety of sizes and no donor site morbidity. Tissue engineering approaches, including pro-angiogenic stem cells and three-dimensional bioprinting, are being explored to enhance the revascularization of nerve grafts and scaffolds.
WIRES MECHANISMS OF DISEASE
(2023)
Article
Chemistry, Analytical
Kayle J. Bender, Yongheng Wang, Chuo Ying Zhai, Zoe Saenz, Aijun Wang, Elizabeth K. Neumann
Summary: This study presents a method for mass spectrometry imaging on undecalcified bones, allowing for the analysis of tissue types and lipidomic profiles in different regions of the spine. It is expected to contribute to understanding the mechanisms underlying bone-related diseases.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Biomedical
Kewa Gao, Jie Li, Hengyue Song, Hesong Han, Yongheng Wang, Boyan Yin, Diana L. Farmer, Niren Murthy, Aijun Wang
Summary: Nanoparticle-based drug delivery systems have the potential to revolutionize medicine, but their impact is limited due to low vascular permeability and rapid clearance by phagocytic cells. This study demonstrates the ability of lipid nanoparticle (LNP) mRNA complexes to deliver mRNA in utero and effectively transfect major organs with low toxicity. Furthermore, it shows that Cas9 mRNA and sgRNA complexed to LNPs can edit fetal organs. These findings suggest a promising strategy for treating various diseases before birth.
BIOACTIVE MATERIALS
(2023)
Article
Pediatrics
Jana Krystofova Mike, Yasmine White, Rachel S. Hutchings, Christian Vento, Janica Ha, Ariana Iranmahboub, Hadiya Manzoor, Anya Gunewardena, Cheryl Cheah, Aijun Wang, Brian D. Goudy, Satyan Lakshminrusimha, Janel Long-Boyle, Jeffrey R. Fineman, Donna M. Ferriero, Emin Maltepe
Summary: Clemastine shows promise in treating demyelinating diseases but has no effect on neurodevelopmental outcomes following global ischemic insult.
Article
Medicine, Research & Experimental
Dake Hao, Lu Lu, Hengyue Song, Yixin Duan, Jianing Chen, Randy Carney, Jian Jian Li, Ping Zhou, Jan Nolta, Kit S. Lam, J. Kent Leach, Diana L. Farmer, Alyssa Panitch, Aijun Wang
Summary: A new generation of collagen-binding extracellular vesicles (EVs) was developed to improve their retention and therapeutic efficacy. The results showed that these collagen-binding EVs had longer in situ retention, suppressed inflammatory responses, and enhanced tissue regeneration.
Review
Developmental Biology
Abd-Elrahman Said Hassan, Yimeng Lina Du, Su Yeon Lee, Aijun Wang, Diana Lee Farmer
Summary: Spina bifida is a common congenital defect that can cause lifelong disability. Advances in genetics and molecular genetics have improved our understanding of this disease. Treatment options, including intrauterine repair and stem cell therapy, have also advanced.
JOURNAL OF DEVELOPMENTAL BIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Dake Hao, Juan-Maria Lopez, Jianing Chen, Alexandra Maria Iavorovschi, Nora Marlene Lelivelt, Aijun Wang
Summary: This review highlights the importance of the extracellular microenvironment and introduces engineering approaches and technologies for controlling cell behavior and tissue regeneration. By engineering different components and monitoring methods, a higher degree of control over cellular activities and behaviors can be achieved, leading to innovative tissue engineering strategies for regenerative medicine.
BIOENGINEERING-BASEL
(2022)
Article
Developmental Biology
Su Yeon Lee, Emma Grace Loll, Abd-Elrahman Said Hassan, Mingyu Cheng, Aijun Wang, Diana Lee Farmer
Summary: Lymphatic malformations are fluid-filled congenital defects of lymphatic channels, occurring in 1 in 6000 to 16000 patients, often alongside other congenital anomalies and vascular malformations. Recent advancements have been made in understanding these malformations, including known molecular and embryological precursors for lymphangiogenesis, as well as gene mutations and dysregulations implicated in their pathogenesis. Current and developing therapies, particularly focused on targeted biotherapeutics, are discussed.
JOURNAL OF DEVELOPMENTAL BIOLOGY
(2022)