Article
Multidisciplinary Sciences
Quanri Zhang, Weiwei Liu, Han Wang, Hao Zhou, Katarzyna Bulek, Xing Chen, Cun-Jin Zhang, Junjie Zhao, Renliang Zhang, Caini Liu, Zizhen Kang, Robert A. Bermel, George Dubyak, Derek W. Abbott, Tsan Sam Xiao, Laura E. Nagy, Xiaoxia Li
Summary: The C-type lectin receptor Mincle plays a crucial role in promoting central nervous system inflammation by sensing danger signals on TH17 cells.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Lin-Li Lv, Cui Wang, Zuo-Lin Li, Jing-Yuan Cao, Xin Zhong, Ye Feng, Jun Chen, Tao-Tao Tang, Hai-Feng Ni, Qiu-Li Wu, Bin Wang, Hui-Yao Lan, Bi-Cheng Liu
Summary: The release of SAP130 from damaged tubular cells triggers macrophage activation through the miRNA-219c/Mincle mechanism, leading to renal necroinflammation. Additionally, negative regulation of Mincle by miR-219c-3p could potentially serve as a novel therapeutic target for AKI.
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Alexey V. Kostarnoy, Petya G. Gancheva, Igor I. Kireev, Andrey I. Soloviev, Bernd Lepenies, Andrey Y. Kulibin, Ekaterina A. Malolina, Olga N. Scheglovitova, Alexey V. Kondratev, Maria V. Sokolova, Daria Vorobyeva, Nadezhda Filippova, Marina R. Kapkaeva, Maria A. Lagarkova, Pavel S. Metalnikova, Evgeniy Riabenko, Daniil A. Grumov, Maxim A. Bobrov, Elena Vasilieva, Boris S. Naroditsky, Alexander L. Gintsburg
Summary: Cellular lipid uptake is a fundamental physiological process, and we have discovered a previously unknown mechanism of lipid cargo uptake mediated by the receptor Mincle. Our study reveals that Mincle tightly binds a range of self-lipids and forms vesicles in the cytoplasm. Knockout of Mincle leads to a significant decrease in lipid endocytosis, suggesting it as a prospective target for disorders of lipid metabolism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Kathryne E. Marks, Stephanie Flaherty, Kristen M. Patterson, Matthew Stratton, Gustavo J. Martinez, Joseph M. Reynolds
Summary: The study shows that activation of TLR2 increases the pathogenicity and migratory capacity of Th17 cells, comparable to IL-23. RNA sequencing revealed differential expression of genes associated with Th17 pathogenicity through the TLR2 pathway, including Ipcef1.
Article
Medicine, Research & Experimental
Matthew Stephens, Keith Keane, Simon Roizes, Shan Liao, Pierre-Yves Von der Weid
Summary: The article introduces a specific DNA aptamer targeting Mincle and discusses its potential in treating inflammatory bowel disease.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Biochemistry & Molecular Biology
Hye-Soo Park, Seunga Choi, Yong-Woo Back, Kang-In Lee, Han-Gyu Choi, Hwa-Jung Kim
Summary: The study found that PGE2 produced by RpfE-activated dendritic cells via the MAPK and cyclooxygenase 2 signaling pathways induces Th1 and Th17 cell responses mainly via the EP4 receptor, contributing to defense against Mycobacterium tuberculosis infection. Moreover, adding optimal amount of PGE2 to IL-2-IL-6-IL-23p19-IL-1 beta is essential for promoting differentiation into Th1/Th17 cells with strong bactericidal activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Robert Nechanitzky, Duygu Nechanitzky, Parameswaran Ramachandran, Gordon S. Duncan, Chunxing Zheng, Christoph Gobl, Kyle T. Gill, Jillian Haight, Andrew C. Wakeham, Bryan E. Snow, Vivian Bradaschia-Correa, Milan Ganguly, Zhibin Lu, Mary E. Saunders, Richard A. Flavell, Tak W. Mak
Summary: Choline acetyltransferase (ChAT) plays a critical role in the pathogenicity of experimental autoimmune encephalomyelitis (EAE), a mouse model of multiple sclerosis (MS). ChAT-deficient Th17 cells in mice resist disease progression and show reduced brain-infiltrating immune cells. ChAT expression in Th17 cells is associated with TCR signaling, Bhlhe40 transcription factor, and increased mRNA levels of Il2, Il17, Il22, and Il23r.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Biochemistry & Molecular Biology
Amy T. Lynch, Chihiro Motozono, Amy J. Foster, Kristel Kodar, Emma M. Dangerfield, Sho Yamasaki, D. Neil Wedlock, Mattie S. M. Timmer, Bridget L. Stocker
Summary: Research has shown that modifying the ester-linkage of trehalose glycolipid to an amide can impact Mincle signaling and enhance immune responses, which was validated in murine and ovine model experiments.
BIOORGANIC CHEMISTRY
(2021)
Article
Immunology
Angela Holder, Jeannine Kolakowski, Chloe Rosentreter, Ellen Knuepfer, Sabine A. F. Jegouzo, Oliver Rosenwasser, Heather Harris, Lotta Baumgaertel, Amanda Gibson, Dirk Werling
Summary: Innate immune receptors form complexes with secondary receptors to activate multiple signaling pathways, regulating homeostasis and immunity. A bovine homologue of Mincle (boMincle) has been characterized to be structurally and functionally similar to the human orthologue (huMincle), although there are some differences. BoMincle is predominantly expressed by myeloid cells, but mRNA transcripts were also detected in granulocytes, B cells, and T cells. BoMincle has the potential to recognize endogenous ligands, suggesting its role in homeostasis in cattle.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Engineering, Biomedical
Aaron T. Hendricksen, Shahrzad Ezzatpour, Anunay J. Pulukuri, Austin T. Ryan, Tatum J. Flanagan, William Frantz, David W. Buchholz, Victoria Ortega, Isaac A. Monreal, Julie M. Sahler, Amy E. Nielsen, Hector C. Aguilar, Rock J. Mancini
Summary: This work presents a synthetic vaccine adjuvant that can adjust its potency in response to temperature changes. The adjuvant enhances vaccine efficacy but can also cause inflammatory side effects like fever, limiting its use. To address this issue, a temperature-sensitive adjuvant is designed to decrease its potency at fever temperatures. The adjuvant exhibits thermophobic behavior and improves the effectiveness of an influenza vaccine.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Takanori Matsumaru, Kodai Sueyoshi, Kana Okubo, Shusuke Fujii, Kasumi Sakuratani, Ryota Saito, Kazunari Ueki, Sho Yamasaki, Yukari Fujimoto
Summary: In this study, new TDE derivatives were synthesized and showed comparable or superior signaling activity to TDS or TDB. The introduction of polar functional groups into the lipid moiety also improved solubility in polar solvents. The results suggest that Mincle tolerates polar functional groups at a certain position of the lipid chain of TDE.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Immunology
Yohei Mikami, Rachael L. Philips, Giuseppe Sciume, Franziska Petermann, Francoise Meylan, Hiroyuki Nagashima, Chen Yao, Fred P. Davis, Stephen R. Brooks, Hong-Wei Sun, Hayato Takahashi, Amanda C. Poholek, Han-Yu Shih, Behdad Afzali, Stefan A. Muljo, Markus Hafner, Yuka Kanno, John J. O'Shea
Summary: MiR-221 and miR-222 play a crucial role in regulating intestinal Th17 cell response, with their expression controlled by various cytokines. Loss of miR-221 and miR-222 expression shifts the gene transcription spectrum of Th17 cells to a proinflammatory signature.
Article
Immunology
Jan Dobes, Osher Ben-Nun, Amit Binyamin, Liat Stoler-Barak, Bergithe E. Oftedal, Yael Goldfarb, Noam Kadouri, Yael Gruper, Tal Givony, Itay Zalayat, Katarina Kovacova, Helena Bohmova, Evgeny Valter, Ziv Shulman, Dominik Filipp, Eystein S. Husebye, Jakub Abramson
Summary: Research has found that Aire(+)MHCII(+) type 3 innate lymphoid cells play a critical role in sensing and presenting Candida albicans, and they have a significant impact on the generation of Candida-specific T(H)17 cells.
Article
Oncology
Lian Zhang, Ping Yang, Jing Wang, Qi Liu, Tian Wang, Yaling Wang, Feng Lin
Summary: MiR-22 expression is significantly downregulated in malignant tissues and circulating T cells of HCC patients, correlating with good prognosis. Overexpression of miR-22 inhibits primary HCC formation and Hepa1-6 subcutaneous tumor growth by promoting Th17 cell differentiation. Injection of miR-22-overexpressing T cells inhibits Hepa1-6 subcutaneous tumor growth through Th17 differentiation promotion.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Chemistry, Organic
Takanori Matsumaru, Kasumi Sakuratani, Saeko Yanaka, Koichi Kato, Sho Yamasaki, Yukari Fujimoto
Summary: Mincle, a C-type lectin receptor, recognizes certain lipid conjugates and activates the innate immune system. The structure-activity relationship analysis revealed that the dimannosyl-fatty acid moiety in the mannosyloxymannitol glycolipid-type structure plays a major role in Mincle recognition.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Immunology
Huafeng Wang, Mengyi Li, Tassanee Lerksuthirat, Bruce Klein, Marcel Wuethrich
INFECTION AND IMMUNITY
(2016)
Article
Microbiology
Ruchuros Inkomlue, Noppadol Larbcharoensub, Patcharee Karnsombut, Tassanee Lerksuthirat, Rangsima Aroonroch, Tassanee Lohnoo, Wanta Yingyong, Pitak Santanirand, Lalana Sansopha, Theerapong Krajaejun
JOURNAL OF CLINICAL MICROBIOLOGY
(2016)
Article
Immunology
Huafeng Wang, Vanessa LeBert, Mengyi Li, Tassanee Lerksuthirat, Kevin Galles, Bruce Klein, Marcel Wuthrich
JOURNAL OF INFECTIOUS DISEASES
(2016)
Article
Cell Biology
Tassanee Lerksuthirat, Rakkreat Wikiniyadhanee, Wasana Stitchantrakul, Sermsiri Chitphuk, Nauljun Stansook, Nut Pipatpanyanugoon, Siwanon Jirawatnotai, Donniphat Dejsuphong
Article
Biochemistry & Molecular Biology
Rakkreat Wikiniyadhanee, Tassanee Lerksuthirat, Wasana Stitchantrakul, Sermsiri Chitphuk, Thanyachai Sura, Donniphat Dejsuphong
Article
Pediatrics
Thipwimol Tim-Aroon, Khunton Wichajarn, Kamornwan Katanyuwong, Pranoot Tanpaiboon, Nithiwat Vatanavicharn, Kullasate Sakpichaisakul, Arthaporn Kongkrapan, Jakris Eu-ahsunthornwattana, Supranee Thongpradit, Kanya Moolsuwan, Nusara Satproedprai, Surakameth Mahasirimongkol, Tassanee Lerksuthirat, Bhoom Suktitipat, Natini Jinawath, Duangrurdee Wattanasirichaigoon
Summary: Sandhoff disease (SD) is an autosomal recessive lysosomal storage disorder caused by deficiency of beta -hexosaminidase B (HEX-B) due to pathogenic variant of human HEXB gene. This study identified potential common variant in HEXB in Thai patients with infantile onset SD and provided data that can aid in rapid molecular confirmation of the disease.
Article
Biotechnology & Applied Microbiology
Duangjai Sittipol, Sudarat Rodpan, Ya'u S. Ajingi, Tassanee Lohnoo, Tassanee Lerksuthirat, Yothin Kumsang, Wanta Yingyong, Pongsak Khunrae, Triwit Rattanarojpong, Kovit Pattanapanyasat, Nujarin Jongruja
Summary: This study identified and characterized a novel hyperthermostable keratinase from microorganisms for efficient feather waste degradation. The isolated keratinase showed high activity and stability at extreme temperatures, making it a potential candidate for future industrial applications in keratin degradation.
Article
Biochemistry & Molecular Biology
Tassanee Lerksuthirat, Rakkreat Wikiniyadhanee, Sermsiri Chitphuk, Wasana Stitchantrakul, Somponnat Sampattavanich, Siwanon Jirawatnotai, Juangjun Jumpathong, Donniphat Dejsuphong
Article
Biochemistry & Molecular Biology
Wararat Chiangjong, Jirawan Panachan, Thitinee Vanichapol, Nutkridta Pongsakul, Pongpak Pongphitcha, Teerapong Siriboonpiputtana, Tassanee Lerksuthirat, Pracha Nuntnarumit, Sarayut Supapannachart, Chantragan Srisomsap, Jisnuson Svasti, Suradej Hongeng, Somchai Chutipongtanate
Summary: HMP-S7, a novel anti-leukemic peptide with 3+ charges and an alpha helical structure, exhibits selective cytotoxic activity against leukemic cells while sparing solid malignancies and normal cells. It induces leukemic cell death by penetrating the plasma membrane to enter the cytoplasm and cause the leakage of lactate dehydrogenase. Further validation in preclinical and clinical studies is needed to confirm its anti-leukemic effects.
Article
Multidisciplinary Sciences
Tassanee Lerksuthirat, Pasinee On-yam, Sermsiri Chitphuk, Wasana Stitchantrakul, David S. Newburg, Ardythe L. Morrow, Suradej Hongeng, Wararat Chiangjong, Somchai Chutipongtanate
Summary: Anticancer peptides (ACPs) are being explored as a new approach for cancer therapy. This study used a sequential procedure to identify candidate ACPs from a computer-generated peptide library inspired by alpha-lactalbumin. In vitro screening identified ALA-A2 as a promising ACP that selectively kills A549 lung cancer cells without side effects and induces autophagy for cancer cell death. This cost-effective approach supports the use of larger computational peptide libraries for ACP research.
Article
Oncology
Sununtha Meksawasdichai, Tassanee Lerksuthirat, Boonsong Ongphiphadhanakul, Chutintorn Sriphrapradang
Summary: This study demonstrates the potential of using Twitter data to understand the perspectives and experiences of patients with thyroid cancer globally, highlighting its value as a data source for understanding patient needs.
Article
Biology
Tassanee Lerksuthirat, Sermsiri Chitphuk, Wasana Stitchantrakul, Donniphat Dejsuphong, Aijaz Ahmad Malik, Chanin Nantasenamat
Summary: This study constructed a classification model for predicting PARP-1 inhibitors for cancer therapy and identified important molecular features in the process. The model showed good performance using the random forest algorithm and various sampling methods. The findings were applied to an online application called PARP1pred, allowing users to predict the biological activity of query compounds.
Article
Public, Environmental & Occupational Health
Sermsiri Chitphuk, Wasana Stitchantraku, Boonsong Ongphiphadhanakul, Patipark Kueanjinda, Tassanee Lerksuthirat
Summary: This study analyzed tweets on Twitter mentioning COVID-19 vaccination in the Thai language and found that the vaccination actions or promotional activities by public figures significantly increased positive sentiment among the public. This suggests that public figures and medical professionals can play a positive role in improving public perceptions of vaccination and should be encouraged to actively support vaccination campaigns.
SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH
(2022)
Article
Biotechnology & Applied Microbiology
Duangjai Sittipol, Pijitra Saelao, Tassanee Lohnoo, Tassanee Lerksuthirat, Yothin Kumsang, Wanta Yingyong, Pongsak Khunrae, Triwit Rattanarojpong, Nujarin Jongruja
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2019)
Article
Microbiology
Tassanee Lerksuthirat, Areeporn Sangcakul, Tassanee Lohnoo, Wanta Yingyong, Thidarat Rujirawat, Theerapong Krajaejun
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2017)