Article
Gastroenterology & Hepatology
Patrick Behrendt, Martina Friesland, Jan-Erik Wissmann, Volker Kinast, Yannick Stahl, Dimas Praditya, Lucas Hueffner, Pia Maria Noerenberg, Birgit Bremer, Benjamin Maasoumy, Jochen Steinmann, Britta Becker, Dajana Paulmann, Florian H. H. Brill, Joerg Steinmann, Rainer G. Ulrich, Yannick Brueggemann, Heiner Wedemeyer, Daniel Todt, Eike Steinmann
Summary: HEV demonstrated resistance to alcohols and alcohol-based disinfectants, with the addition of phosphoric acid being essential for virucidal activity. These findings are crucial for guiding improved hygiene measures to prevent HEV transmission.
JOURNAL OF HEPATOLOGY
(2022)
Article
Microbiology
Catherine Hennechart-Collette, Oceane Dehan, Audrey Fraisse, Sandra Martin-Latil, Sylvie Perelle
Summary: Viruses, including hepatitis A (HAV), hepatitis E (HEV), and human norovirus, are major concerns in food hygiene. Existing ISO 15216 approved methods fail to validate the detection of HAV and human norovirus in food products, such as fish, jeopardizing product safety. This study aims to develop a rapid and sensitive method for detecting these viruses in fish. The method, validated according to ISO 16140-4, demonstrated efficient recovery and accurate LOD values, making it suitable for routine diagnostics in various fish products.
Review
Virology
Chye Phing Teh, Jack Bee Chook, Yun Fong Ngeow, Tommy Yuh Koon Tong, Kok Keng Tee, Jan Jin Bong, Rosmawati Mohamed
Summary: Current treatment strategies for chronic hepatitis B virus infection focus on long-term suppression of viral replication, with routine monitoring of serum HBV DNA levels being crucial. A study on 53 qPCR assays over the past two decades found that only 17% achieved a coverage of >= 80%, with about 40% covering less than 20% of the 9864 HBV genomes analyzed. The findings highlight the need for improving qPCR designs for accurate quantification of HBV DNA based on updated genome data.
REVIEWS IN MEDICAL VIROLOGY
(2021)
Editorial Material
Biology
Altaira D. Dearborn, Ashish Kumar, Joseph Marcotrigiano
Summary: A domain in the ORF1 polyprotein of the hepatitis E virus, previously believed to be a protease, is actually a zinc-binding domain.
Article
Biotechnology & Applied Microbiology
Kairui Wang, Libing Liu, Jinfeng Wang, Xiaoxia Sun, Qingan Han, Cang Zhou, Xiangdong Xu, Jianchang Wang
Summary: This study established a new RT-ddPCR method for detecting Hepatitis E virus (HEV) in raw pork livers. The results showed that the prevalence of HEV in China was 2.24%, with higher detection rates in bio-safety disposal centers than in slaughterhouses and retail markets.
Article
Virology
Zhongyao Qian, Chao Cong, Yi Li, Yanhong Bi, Qiuxia He, Tengyuan Li, Yueping Xia, Liangheng Xu, Houfack K. Mickael, Wenhai Yu, Jiankun Liu, Daqiao Wei, Fen Huang
Summary: This study compared the proteomic differences in HEV-infected HepG2 cells supplemented with fetal bovine serum (FBS) and serum from women in their third trimester of pregnancy (PS) using isobaric tags for relative and absolute quantification technology. The results showed that PS supplementation adjusted more host genes and signaling pathways in HEV-infected cells compared to FBS supplementation. HEV-infected cells supplemented with PS exhibited more immune-related proteins. These findings provide insights into the pathogenesis of HEV infection in pregnant women.
Article
Virology
Putu Prathiwi Primadharsini, Shigeo Nagashima, Masaharu Takahashi, Kazumoto Murata, Hiroaki Okamoto
Summary: Combination therapy with ritonavir and ribavirin significantly improves the inhibition of hepatitis E virus (HEV) growth, even in cells with robust virus production. This study provides insight into the potential of using drugs that block different steps in the HEV life cycle as a novel treatment strategy for chronic hepatitis E.
Article
Food Science & Technology
Catherine Hennechart-Collette, Lisa Fourniol, Audrey Fraisse, Sandra Martin-Latil, Sylvie Perelle
Summary: This study aimed to characterize a proteinase K method for the detection of human norovirus, hepatitis A virus, and hepatitis E virus in artificially contaminated dairy products. The method proved to be suitable for routine diagnostic needs in dairy products.
Article
Public, Environmental & Occupational Health
Erwin Nagelkerke, Wouter A. Hetebrij, Jaap M. Koelewijn, Jannetje Kooij, Anne-Merel R. van der Drift, Rudolf F. H. J. van der Beek, Eline F. de Jonge, Willemijn J. Lodder
Summary: Wastewater surveillance programs have been established or upscaled in many countries since 2020, providing a cost-effective way of monitoring infectious disease pathogens. However, systematic shifts in standard curves over time can affect the reliability and usefulness of wastewater surveillance. This study demonstrates that using five days of standard curve data for quantification can minimize errors.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Microbiology
Tiina Korkea-aho, Tom Wiklund, Christine Engblom, Anssi Vainikka, Satu Viljamaa-Dirks
Summary: The study validated a qPCR assay for rapid and accurate detection of Saprolegnia parasitica in aquaculture, showing high sensitivity and specificity in both experimental and field applications. The method can be used to detect S. parasitica in water samples and parasites in fish mucus, providing an effective means for disease management and surveillance in aquaculture.
Article
Biochemistry & Molecular Biology
Preeti Hooda, Mohd Ishtikhar, Shweta Saraswat, Pooja Bhatia, Deepali Mishra, Aditya Trivedi, Rajkumar Kulandaisamy, Soumya Aggarwal, Manoj Munde, Nemat Ali, Abdullah F. AlAsmari, Mohd A. Rauf, Krishna K. Inampudi, Deepak Sehgal
Summary: Hepatitis E virus (HEV) is a pathogen that spreads through fecal contaminated drinking water, commonly found in South Asian countries. The MTase enzyme of HEV is able to methylate the viral RNA and protect it from degradation. This study cloned and studied the MTase protein of HEV genotype 1, finding its binding affinity with magnesium ions and its role in enzyme activity.
Article
Food Science & Technology
La'Chia Harrison, Thais De Melo Ramos, Xi Wu, Erin DiCaprio
Summary: The study found that ground pork and pig liver are potential sources of HEV, with a high prevalence of HEV infection in pork products sold in the United States. A processing method for detecting HEV in pork products was developed in the study.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Alexia Bordigoni, Anne Motte, Herve Tissot-Dupont, Philippe Colson, Christelle Desnues
Summary: A rapid quantitative PCR detection test for HPV16 and HPV18 oncogenes has been developed, which can be used as a complement to traditional HPV tests. The test can specifically validate the presence of HPV16 and/or HPV18, as well as monitor patients with confirmed infection and at risk of developing lesions.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Julia Melchert, Helena Radbruch, Leif G. Hanitsch, Sally A. Baylis, Jorn Beheim-Schwarzbach, Tobias Bleicker, Jorg Hofmann, Terry C. Jones, Christian Drosten, Victor M. Corman
Summary: This study used high-throughput sequencing techniques, including targeted enrichment, to investigate the sequence diversity and intra-host diversity of HEV. Potential novel subtypes were identified in blood and ancient livestock samples, and ribavirin resistance mutations and HEV compartmentalization were found in a patient.
JOURNAL OF CLINICAL VIROLOGY
(2023)
Article
Clinical Neurology
Miriam Fritz-Weltin, Lisa Niedermeier, Estelle Frommherz, Nora Isenmann, Benedikt Csernalabics, Tobias Boettler, Christoph Neumann-Haefelin, Dominique Endres, Marcus Panning, Benjamin Berger
Summary: This retrospective study systematically screened 104 patients with Bell's palsy for acute HEV infection, but found no association between acute HEV infection and Bell's palsy. However, a high prevalence of anti-HEV IgG in Bell's palsy patients was observed, which increased with age.
EUROPEAN JOURNAL OF NEUROLOGY
(2022)
Article
Biochemical Research Methods
Weili Liu, Hong Zhao, Zhigang Qiu, Min Jin, Dong Yang, Qunying Xu, Hua Feng, Junwen Li, Zhigiang Shen
JOURNAL OF MICROBIOLOGICAL METHODS
(2020)
Article
Environmental Sciences
Sicong Su, Chenyu Li, Jiping Yang, Qunying Xu, Zhigang Qiu, Bin Xue, Shang Wang, Chen Zhao, Zhonghai Xiao, Jingfeng Wang, Zhiqiang Shen
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2020)
Article
Environmental Sciences
Bin Xue, Kun Dai, Xi Zhang, Shang Wang, Chenyu Li, Chen Zhao, Xiaobo Yang, Zhuge Xi, Zhigang Qiu, Zhiqiang Shen, Jingfeng Wang
Article
Ecology
Min Jin, Lu Liu, Da-ning Wang, Dong Yang, Wei-li Liu, Jing Yin, Zhong-wei Yang, Hua-ran Wang, Zhi-gang Qiu, Zhi-qiang Shen, Dan-yang Shi, Hai-bei Li, Jian-hua Guo, Jun-wen Li
Article
Engineering, Environmental
Chengshi Ding, Min Jin, Jing Ma, Zhaoli Chen, Zhiqiang Shen, Dong Yang, Danyang Shi, Weili Liu, Meiling Kang, Jingfeng Wang, Junwen Li, Zhigang Qiu
Summary: This study demonstrates that nano-Al2O3 can serve as a carrier mediating transduction-like ARG transformation, preventing degradation of ARGs and facilitating their transmembrane transport, which is of significant importance for global public health.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Kun Zhou, Weili Liu, Zhaoli Chen, Dong Yang, Zhigang Qiu, Hua Feng, Chao Li, Min Jin, Junwen Li, Qunying Xu, Zhiqiang Shen
Summary: The study found that different types of drinking water have an impact on the gut microbiota of mice, with mineral water as a culture medium potentially contributing to the progressive increase in gut microbiota diversity, particularly dominated by the Bacteroidetes taxa.
JOURNAL OF WATER AND HEALTH
(2021)
Article
Environmental Sciences
Danyang Shi, Hui Ma, Jing Miao, Weili Liu, Dong Yang, Zhigang Qiu, Zhiqiang Shen, Jing Yin, Zhongwei Yang, Huaran Wang, Haibei Li, Zhengshan Chen, Junwen Li, Min Jin
Summary: This study conducted a one-year surveillance of human enteric viruses in four representative water matrices, revealing a correlation between the detection frequencies of enteric viruses in different water sources and their prevalence in clinical PCR assay. The levels of HRVs and HuNoVsGII in urban rivers may mirror the local virus prevalence.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Microbiology
Hai-bei Li, Ai-ming Hou, Tian-jiao Chen, Dong Yang, Zheng-shan Chen, Zhi-qiang Shen, Zhi-gang Qiu, Jing Yin, Zhong-wei Yang, Dan-yang Shi, Hua-ran Wang, Jun-wen Li, Min Jin
Summary: UV exposure may decrease susceptibility to certain antibiotics in bacteria, leading to increased antibiotic resistance and posing potential threats to the environment and human health.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Chengshi Ding, Jing Ma, Wanxiang Jiang, Hanyu Zhao, Mengmeng Shi, Guoqing Cui, Tongdi Yan, Qi Wang, Junwen Li, Zhigang Qiu
Summary: This study demonstrates for the first time the distribution of ARGs in Chironomidae larvae and shows their transmission to downstream predators through the food chain. Conjugative transfer plays a crucial role in ARG spread, with Escherichia and Flavobacterium identified as the main host bacteria of ARGs.
ENVIRONMENTAL POLLUTION
(2021)
Article
Chemistry, Physical
Chenyu Li, Bin Xue, Shang Wang, Xi Zhang, Chen Zhao, Xiaobo Yang, Run Zhao, Lin Dai, Shengqi Su, Haoqi Xu, Zhiqiang Shen, Zhigang Qiu, Jingfeng Wang
Summary: The study established an innovative ultrasound-assisted electrochemical oxidation (UAEO) digestion method to analyze the heavy metal (HM) contamination level of dairy slurry, showing that optimal UAEO operating parameters can significantly affect digestion efficiency. The results demonstrated high digestion efficiency for Cu and Zn with the optimal parameters, confirming the fast and convenient nature of UAEO method with promising application prospects.
Article
Environmental Sciences
Shuqing Zhou, Min Jin, Rong Tan, Zhiqiang Shen, Jing Yin, Zhigang Qiu, Zhengshan Chen, Danyang Shi, Haibei Li, Zhongwei Yang, Huaran Wang, Zhixian Gao, Junwen Li, Dong Yang
Summary: The study demonstrates that CTAB-functionalized rGO-Fe3O4 composites have efficient adsorption capabilities for human enteric viruses and SARS-CoV-2, and can be used in complex water environments. Additionally, the composite can help concentrate virus particles before qPCR analysis, significantly improving detection sensitivity.
Article
Chemistry, Analytical
Zhuosong Cao, Chenyu Li, Xiaobo Yang, Shang Wang, Xi Zhang, Chen Zhao, Bin Xue, Chao Gao, Hongrui Zhou, Yutong Yang, Zhiqiang Shen, Feilong Sun, Jingfeng Wang, Zhigang Qiu
Summary: This study presents a method based on chronoamperometry for the rapid and quantitative detection of live E. coli. The method shows good correlation between the electrochemical indicator signals and the concentration of E. coli, with a detection time of less than 5 minutes and a detection range of 10(4)-10(8) CFU/mL. The method also demonstrates good repeatability, stability, and sensitivity.
Article
Environmental Sciences
Chengshi Ding, Dong Yang, Jing Ma, Min Jin, Zhiqiang Shen, Danyang Shi, Zhongjing Tian, Meiling Kang, Junwen Li, Zhigang Qiu
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2020)
Article
Chemistry, Multidisciplinary
Danhui Zhao, Kui Lin, Lanhui Wang, Zhigang Qiu, Xin Zhao, Kunze Du, Lifeng Han, Fei Tian, Yanxu Chang
Article
Engineering, Environmental
Jing Miao, Han-Ji Jiang, Zhong-Wei Yang, Dan-yang Shi, Dong Yang, Zhi-Qiang Shen, Jing Yin, Zhi-Gang Qiu, Hua-Ran Wang, Jun-Wen Li, Min Jin
ENVIRONMENTAL SCIENCE-WATER RESEARCH & TECHNOLOGY
(2019)
