Article
Agriculture, Dairy & Animal Science
Barbara Salerno, Maddalena Furlan, Raffaella Sabatino, Andrea Di Cesare, Marta Leati, Marcello Volanti, Lisa Barco, Massimiliano Orsini, Carmen Losasso, Veronica Cibin
Summary: This study analyzed a broiler farm that has never used antibiotics and found that not using antibiotics does not fully reduce the load of antibiotic resistance genes (ARGs) in meat processing environments. In addition, other sources of ARGs, including the production phase and breeding stage, should be considered.
Article
Environmental Sciences
Animesh Tripathi, Dinesh Kumar, Priyank Chavda, Dalip Singh Rathore, Ramesh Pandit, Damer Blake, Fiona Tomley, Madhvi Joshi, Chaitanya G. Joshi, Suresh Kumar Dubey
Summary: Poultry farming in South and Southeast Asian economies is intensifying to meet the increasing demand for protein, but this intensification leads to increased use of antimicrobial drugs and the spread of antimicrobial resistance genes (ARGs), posing a threat to food chains. This study investigates the transmission of ARGs from chicken litter to soil and plants, and demonstrates that ARGs can be transmitted from litter to plant systems. The most common ARGs detected were cmx, ErmX, ErmF, lnuB, TEM-98, and TEM-99, and the common microorganisms included Escherichia coli, Staphylococcus aureus, Enterococcus faecium, Pseudomonas aeruginosa, and Vibrio cholerae. The findings highlight the risks posed to the environment by antimicrobial treatment of poultry and provide valuable knowledge for formulating intervention strategies to prevent ARGs transmission and improve understanding of the impacts on human and environmental health.
ENVIRONMENTAL POLLUTION
(2023)
Article
Engineering, Environmental
Shuang Peng, Hongyan Zhang, Dan Song, Hong Chen, Xiangui Lin, Yiming Wang, Lidong Ji
Summary: With the rapid development of livestock and poultry breeding industries, pollution problems caused by animal feces discharge have become increasingly severe. This study investigated nutrients content, antibiotics, heavy metals, antibiotic resistance genes (ARGs), and mobile genetic elements (MGEs) in livestock and poultry feces from different scales of farms in Northwest China. The results showed that pig and chicken feces had higher levels of nutrients, antibiotics, heavy metals, ARGs, and MGEs compared to cattle and sheep feces. Chlortetracycline hydrochloride and doxycycline hyclate were the most commonly used antibiotics, detected at high concentrations in broiler, layer, and pig feces. The study also found strong correlations among different ARGs and between ARGs and MGEs, indicating the risk of horizontal gene transfer. The use of antibiotics and heavy metals should be strictly regulated, especially in chicken and pig farms, to reduce potential risks to the environment.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Environmental Sciences
Liping Ma, Huiying Yang, Lei Guan, Xiaoyu Liu, Tong Zhang
Summary: Chlorination process can significantly decrease both abundance and diversity of total ARGs, but with limited removal rates for opportunistic pathogens, such as E. coli isolates. Core subtypes of ARGs were identified and found to persist during chlorination for both total microbes and E. coli. Additionally, metagenomic binning strategies revealed enrichment of certain ARG-carrying genomes after chlorination.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Infectious Diseases
David Baunoch, Natalie Luke, Dakun Wang, Annah Vollstedt, Xinhua Zhao, Dicken S. C. Ko, Shuguang Huang, Patrick Cacdac, Larry T. Sirls
Summary: This study aimed to analyze the concordance between antibiotic resistance genes and antibiotic susceptibility results in urine samples from patients with urinary tract infections. The overall concordance rate between the presence of ABR genes and antimicrobial susceptibility was 60%, with variations across different antibiotics. However, certain antibiotics showed significantly lower concordance rates in polymicrobial infections.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Environmental Sciences
Xing Xu, Weidong Zhou, Chuanqi Xie, Yinchu Zhu, Wensheng Tang, Xin Zhou, Hua Xiao
Summary: The abundance and diversity of bacteria, gas pollutants, and antibiotic resistance genes (ARGs) in the air of poultry farms are similar inside and downwind of the farm. Pathogens like Staphylococcus and Corynebacterium were found both inside and outside the chicken house. Airborne bacteria can spread through wind and significantly contribute to the profiles of ARGs in bioaerosols.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Yanan Wang, Na Lyu, Fei Liu, William J. Liu, Yuhai Bi, Zewu Zhang, Sufang Ma, Jian Cao, Xiaofeng Song, Aiping Wang, Gaiping Zhang, Yongfei Hu, Baoli Zhu, George Fu Gao
Summary: The study reveals the significant impact of the live poultry trade on the diversity of antimicrobial resistance genes in LPM workers, chickens, and environments in China. The LPMs possess a greater diversity of antimicrobial resistance genes, some of which are also carried by human clinical pathogens.
ENVIRONMENT INTERNATIONAL
(2021)
Article
Microbiology
Zhangming Pei, Faizan Ahmed Sadiq, Xiao Han, Jianxin Zhao, Hao Zhang, R. Paul Ross, Wenwei Lu, Wei Chen
Summary: Prophage integration, release, and dissemination have various effects on host bacteria, with almost all lactobacilli containing prophage fragments and 64.1% having intact prophages. Prophages are unevenly distributed among Lactobacillus species, with multihabitat species retaining more prophages. The high genome diversity of Lactobacillus prophages and the presence of antibiotic resistance genes suggest their potential role in phenotypic resistance in Lactobacillus plantarum.
Article
Biochemical Research Methods
Sunjae Hwang, Jungil Choi
Summary: Antibiotic resistance poses a global threat to human health. A rapid antibiotic susceptibility test (AST) is crucial for appropriate antibiotic prescriptions for patients infected with antibiotic-resistant bacteria. This study introduces a rapid AST platform that utilizes low-density bacterial samples, concentrates bacterial cells, and performs AST on a single microfluidic chip. The platform allows quick detection of antibiotic resistance in three hours and can be used for cell-based drug testing of low-concentration bacterial samples.
Article
Infectious Diseases
Angelika Sacher-Pirklbauer, Daniela Klein-Joebstl, Dmitrij Sofka, Anne-Beatrice Blanc-Potard, Friederike Hilbert
Summary: Escherichia coli isolated from meat of different animal species may contain antimicrobial resistance genes, posing a potential threat to human health. However, the distribution of resistance genes was found to be similar across different meat species, and the variety of resistance genes detected was relatively low.
Article
Food Science & Technology
Rajae Zahli, Ann Karolin Scheu, Jamal Abrini, Jose L. Copa-Patino, Amajoud Nadia, Skali Senhaji Nadia, Juan Soliveri
Summary: The prevalence of Salmonella in poultry samples was found to be 9.84%, with major serovars including S. Hadar, S. Typhimurium, and S. Chester. 80.95% of Salmonella isolates were resistant to at least one antibiotic. The study also detected nine different MLST sequence types, with ST1954 being the most common.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Infectious Diseases
Meiyao Che, Tina Birk, Lisbeth Truelstrup Hansen
Summary: This study reveals the presence and transmission of extended-spectrum cephalosporin-resistant Escherichia coli in the Danish broiler production system, highlighting the potential for these resistant bacteria to enter the food supply chain.
Review
Biochemical Research Methods
Artur Ruszczak, Simona Bartkova, Marta Zapotoczna, Ott Scheler, Piotr Garstecki
Summary: The application of droplet-based methods in antimicrobial resistance research allows for faster detection, increased sensitivity, characterization of heterogeneity in bacterial populations' response to antibiotics, and expanded screening of antibiotic combinations. This article discusses the key steps and parameters of droplet-based experiments, reviews recent findings achieved with these methods, and highlights their advantages and capacity to provide new insights into the problem of antimicrobial resistance.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Ruijie Zhang, Xuming Xu, Yitao Lyu, Ying Zhou, Qian Chen, Weiling Sun
Summary: This study investigated the effects of antibiotics and engineered nanoparticles on denitrification in natural aquatic environment. The results showed that antibiotics inhibited the reduction of NO3--N, while engineered nanoparticles promoted denitrification. In binary systems, engineered nanoparticles alleviated the inhibitory effect of antibiotics. The addition of antibiotics and/or engineered nanoparticles also changed the bacterial community structure.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Miguel Semedo, Bongkeun Song
Summary: The intensification of the poultry industry can lead to the increased spread of antibiotic resistance genes (ARGs) in the environment. This study examined the abundance and diversity of ARGs and N cycling genes in sediment microbial communities affected by poultry industry wastewater. The results showed higher abundances of ARGs, especially near the wastewater discharge, indicating potential negative impacts on ecosystem health.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Agriculture, Dairy & Animal Science
Dagmara Stepien-Pysniak, Tomasz Hauschild, Marta Dec, Agnieszka Marek, Renata Urban-Chmiel, Urszula Kosikowska
Summary: Bacterial infections of yolk sacs in chicks can lead to high mortality rates and economic losses in poultry production. This study aimed to characterize enterococci isolated from yolk sac infections in broiler chickens from Poland and the Netherlands, finding important virulence factors and genetic relatedness among isolates.
Article
Agriculture, Dairy & Animal Science
Agnieszka Marek, Ewelina Pyzik, Dagmara Stepien-Pysniak, Marta Dec, Lukasz S. Jarosz, Anna Nowaczek, Magdalena Sulikowska
Summary: Staphylococcus bacteria are commonly found on warm-blooded animals, with some species causing opportunistic infections in poultry. This study aimed to analyze the biofilm-forming potential and occurrence of biofilm-associated genes in coagulase-negative Staphylococcus strains isolated from poultry. The majority of strains showed biofilm production capacity, with varying levels of strength demonstrated. Some key biofilm-associated genes were detected in a subset of strains.
Review
Food Science & Technology
Monika Jamiol, Jacek Wawrzykowski, Marta Dec, Agata Wilk, Michal Czelej
Summary: This review provides an overview of the extraction techniques, bioactive compounds, and mechanisms of action of selected Rhodiola species, with a focus on their free radical scavenging properties. It critically evaluates the literature on the roots and rhizomes of Rhodiola species, particularly R. rosea, R. imbricata, R. crenulata, R. kirilowii, and R. heterodonta. The review presents a list of the most important chemical compounds, mainly phenolic compounds and essential oils, and compares different extraction methods qualitatively and quantitatively. The antioxidant effects of Rhodiola species in vitro and in vivo on mouse and rat are also analyzed.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Microbiology
Marta Dec, Dagmara Stepien-Pysniak, Klaudiusz Szczepaniak, Barbara Turchi, Renata Urban-Chmiel
Summary: Exotic reptiles are increasingly being bred as pets, but close contact between reptiles and their owners can lead to the transmission of zoonotic pathogens. This study examined E. coli isolates from captive reptiles, revealing that the incidence of E. coli was low but strains carried virulence genes and had the potential to cause disease. Therefore, good hygiene practices should be followed when in contact with reptiles.
Article
Agriculture, Dairy & Animal Science
Jaroslaw Wilczynski, Dagmara Stepien-Pysniak, Danuta Wystalska, Andrzej Wernicki
Summary: This study aimed to characterize the virulence factors of APEC isolated from poultry in Poland and investigate the correlation between lesion severity, the presence of virulence genes, and the E. coli serogroup. The results revealed a great variety of APEC strains in the poultry population, with different serotypes exhibiting distinct virulence characteristics and symptom presentations.
Review
Environmental Sciences
Joanna Stec, Urszula Kosikowska, Mariola Mendrycka, Dagmara Stepien-Pysniak, Paulina Niedzwiedzka-Rystwej, Dominika Bebnowska, Rafal Hrynkiewicz, Joanna Zietara-Wysocka, Ewelina Grywalska
Summary: Infections caused by exposure to opportunistic pathogens during recreational water use can have serious health consequences, especially in developing countries. The quality of water in natural reservoirs is often low, which poses a threat to human health. Therefore, the quality of recreational waters is closely monitored in many jurisdictions. Antimicrobial resistance among opportunistic pathogens remains an important issue, requiring measures to reduce chemical stressors and understand the mechanisms of antibiotic-resistant gene spread.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Review
Infectious Diseases
Renata Urban-Chmiel, Agnieszka Marek, Kinga Wieczorek, Marta Dec, Dagmara Stepien-Pysniak, Anna Nowaczek, Jacek Osek
Summary: This article discusses the global problem of multi-drug resistance among bacteria, explores the mechanisms of bacterial resistance, and provides statistical data on the occurrence of drug resistance among selected bacteria in different regions. The article also provides detailed information on the detection of drug resistance genes for specific groups of antibiotics.
Article
Microbiology
Marta Dec, Magdalena Zajac, Andrzej Puchalski, Klaudiusz Szczepaniak, Renata Urban-Chmiel
Summary: This study aimed to determine the incidence of Salmonella in pet reptiles in Poland and examined the biochemical characteristics, serotype, antimicrobial susceptibility, and pathogenic and zoonotic potential of Salmonella isolates. The results showed that pet reptiles kept in Poland are a significant reservoir of Salmonella and special hygiene rules are necessary to prevent the risk of salmonellosis.
Article
Biochemistry & Molecular Biology
Sylwia Andrzejczuk, Monika Cygan, Dominik Dluski, Dagmara Stepien-Pysniak, Urszula Kosikowska
Summary: Antimicrobial resistance in Staphylococcus spp. in the nasopharynx of pregnant women can lead to treatment failure and prophylaxis failure. The resistance is mainly encoded by plasmids or chromosomes. The most prevalent species were S. aureus and S. epidermidis, which showed high diversity in resistance profiles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Ewelina Pyzik, Renata Urban-Chmiel, Agnieszka Chalabis-Mazurek, Sylwester Swiatkiewicz, Anna Arczewska-Wlosek, Tomasz Schwarz, Jose L. Valverde Piedra
Summary: The research evaluated the impact of an experimental diet with rye and xylanase on the intestinal microbiome of broiler chickens in different rearing systems, finding abundant bacteria in the gut of chickens from both housing systems. Escherichia coli, Enterococcus spp., Proteus spp., Campylobacter spp., and Staphylococcus spp. were commonly isolated, with varying levels of antibiotic resistance observed in different bacteria among chickens from different farming methods. The study suggests the need to control microbiome in favor of beneficial bacteria in the gastrointestinal tract due to high antibiotic resistance among pathogens.
ANNALS OF ANIMAL SCIENCE
(2021)
