期刊
BEILSTEIN JOURNAL OF NANOTECHNOLOGY
卷 8, 期 -, 页码 1494-1501出版社
BEILSTEIN-INSTITUT
DOI: 10.3762/bjnano.8.149
关键词
atomic force microscopy; C60 fullerene; cisplatin; comet assay; computer simulation; dynamic light scattering; flow cytometry; human lymphocytes; toxicity in vitro
资金
- Academician Platon Kostyuk Foundation
- STCU [6256]
The self-organization of C-60 fullerene and cisplatin in aqueous solution was investigated using the computer simulation, dynamic light scattering and atomic force microscopy techniques. The results evidence the complexation between the two compounds. The genotoxicity of C-60 fullerene, Cis and their complex was evaluated in vitro with the comet assay using human resting lymphocytes and lymphocytes after blast transformation. The cytotoxicity of the mentioned compounds was estimated by Annexin V/ PI double staining followed by flow cytometry. The results clearly demonstrate that water-soluble C-60 fullerene nanoparticles (0.1 mg/mL) do not induce DNA strand breaks in normal and transformed cells. C-60 fullerene in the mixture with Cis does not influence genotoxic Cis activity in vitro, affects the cell-death mode in treated resting human lymphocytes and reduces the fraction of necrotic cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据