Review
Biochemistry & Molecular Biology
Shahzad Nawaz Syed, Bernhard Bruene
Summary: This review discusses the potential of MicroRNAs as tools for cancer diagnosis, prognosis, and therapy. The release of miRs into the tumor microenvironment can have both pro- and anti-tumor effects, depending on their source and target cells. The understanding of exosomal and non-exosomal miRs in cancer has provided new insights for potential therapeutic strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Yamei Zhang, Junying Liu, Xin Wang, Jinfeng Zhang, Chenchen Xie
Summary: The study demonstrates that sensory neurons transfer miR-23a encapsulated in EVs to activate M1 macrophages and exacerbate neuropathic pain following peripheral nerve injury. Targeting detrimental miRNA in sensory neurons can alleviate chronic neuropathic pain after nerve trauma.
Article
Neurosciences
Ramin Raoof, Christian Martin Gil, Floris P. J. G. Lafeber, Huub de Visser, Judith Prado, Sabine Versteeg, Mirte N. Pascha, Anne L. P. Heinemans, Youri Adolfs, Jeroen Pasterkamp, John N. Wood, Simon C. Mastbergen, Niels Eijkelkamp
Summary: This study reveals the crucial role of macrophages in maintaining OA pain independently from joint damage. Sensory neurons innervate damaged knee joints, shaping DRG macrophages into an M1 phenotype. Inhibiting M1-like macrophages in the DRG can resolve persistent OA pain.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Cell Biology
Xuefeng Yang, Shuang Cai, Yue Shu, Xun Deng, Yuanwei Zhang, Nian He, Lei Wan, Xu Chen, Yan Qu, Shouyang Yu
Summary: The study showed an increase in the proportion of M2 macrophages in gastric cancer tissues, where M2 macrophages promoted cell proliferation and tumorigenesis through exosomal miR-487a targeting TIA1. These findings may contribute to the development of novel diagnostic and therapeutic methods for gastric cancer.
Article
Biochemistry & Molecular Biology
Song-Yi Choi, Su-Hyung Hong, Heon-Jin Lee
Summary: This study analyzed exosomal miRNA sequencing data from PMA-treated U937 cells and found that miR-24 was highly expressed in exosomes and associated with alpha-tubulin and hnRNP-E1 proteins. Inactivating these proteins resulted in a significant reduction of exosomal miR-24. The findings suggest that miR-24 is transported into exosomes from activated macrophages with the support of alpha-tubulin and hnRNP-E1.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Immunology
Haruki Iwai, Koji Ataka, Hajime Suzuki, Ashis Dhar, Eriko Kuramoto, Atsushi Yamanaka, Tetsuya Goto
Summary: The majority of macrophages appearing after nerve injury are tissue-resident cells that directly contact damaged neurons in an M2 phenotype, suggesting a tissue-protective role.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Clinical Neurology
Chengcheng Zhao, Yuefei Deng, Yi He, Xianjian Huang, Chuanfang Wang, Weiping Li
Summary: Activated microglia can inhibit neurite outgrowth and synapse recovery following traumatic brain injury through exosome-mediated mechanisms. Exosomes derived from stretch-injured microglia were shown to be absorbed by neurons and decrease protein levels associated with neurite outgrowth and synapse recovery, ultimately impairing motor coordination in mice after injury. Overexpression of miR-5121 in these exosomes partly reversed the suppression of neurite outgrowth and synapse recovery, indicating a potential therapeutic target for improving outcomes following TBI.
Article
Agriculture, Multidisciplinary
Zhong-Hao Ji, Fei Gao, Wen-Yin Xie, Hong-Yu Wu, Wen-Zhi Ren, Bao Yuan
Summary: This study identified and isolated exosomes derived from mammary epithelial cells in a mastitis cell model and found that high expression of miR-221-3p in these exosomes promotes M1 macrophage polarization. Mechanistically, miR-221-3p regulates macrophage polarization by targeting Igf2bp2. This molecular regulatory axis may serve as a potential target for mastitis treatment.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Mengqi Zhang, Qian Wu, Mimi Tang, Zhuohui Chen, Haiyue Wu
Summary: In this study, it was found that inhibition of miR-3613-3p can enhance the function of BMECs and reduce neuronal apoptosis under oxygen-glucose deprivation conditions. Additionally, interfering with miR-3613-3p expression in BMSCs can reduce its enrichment in exosomes and promote M2 polarization of microglia, thereby decreasing neuronal apoptosis.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2023)
Article
Biology
Adria Razzauti, Patrick Laurent
Summary: Cilia are sensory organelles protruding from cell surfaces that can release extracellular vesicles (EVs) in some organisms. These EVs are formed by ciliated sensory neurons, with release from both the cilia tip and periciliary membrane compartment. The formation and release of EVs help maintain cilia composition.
Review
Medicine, Research & Experimental
Kowsar Amirzadeh Gougheri, Armin Ahmadi, Mohadeseh Ghafuri Ahmadabadi, Amirhesam Babajani, Ghasem Yazdanpanah, Soheyl Bahrami, Mohammad Hassani, Hassan Niknejad
Summary: Heart diseases are the primary cause of mortality and morbidity worldwide. Exosomes, as a new emerging agent, play significant roles in the diagnosis, treatment, and prognosis of heart diseases.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Genetics & Heredity
Xirui Ma, Huifang Liu, Jing Zhu, Caoxu Zhang, Yajie Peng, Ziming Mao, Yu Jing, Fengling Chen
Summary: MiR-185-5p has been identified as a negative regulator in pro-inflammatory activation of macrophages, exerting anti-inflammatory effects through the CDC42/JNK pathway.
Article
Medicine, Research & Experimental
Zhijian Zhang, Hanzhi Chen, Leting Zhou, Cheng Li, Guoyuan Lu, Liang Wang
Summary: This study investigated the mechanism of tubule injury in acute kidney injury (AKI). In an AKI mouse model, tubular injury was observed and linked to macrophage infiltration. Blocking exosome production improved tubular injury, and it was found that exosomal miR-155 mediated communication between activated macrophages and injured tubules, leading to AKI progression. This study provides insights into the pathophysiology of AKI and offers a potential therapeutic strategy for kidney diseases.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Kou-Gi Shyu, Bao-Wei Wang, Wei-Jen Fang, Chun-Ming Pan, Chiu-Mei Lin
Summary: The expression of macrophage-derived exosomal MALAT1 is increased by high glucose levels, which in turn suppresses the expression of miR-150-5p and promotes resistin expression in macrophages. Additionally, macrophage-derived exosomal MALAT1 may serve as a potential therapeutic target for vascular disease in diabetes mellitus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Shilu Huang, Liang Liu, Zhipeng Xu, Xinglei Liu, Anyi Wu, Xiaopei Zhang, Zengyang Li, Suwen Li, Yongdong Li, Jiaqi Yuan, Shan Cheng, Haoran Li, Jun Dong
Summary: GSCs-derived exosomal miR-6733-5p induces M2 polarization of TAMs by activating the AKT signaling pathway, promoting self-renewal and stemness of GSCs, and enhancing malignant behaviors of GBM. Targeting GSCs exosomal miR-6733-5p may provide a potential new strategy against GBM.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Valentina Vacca, Sara Marinelli, Federica De Angelis, Daniela F. Angelini, Eleonora Piras, Luca Battistini, Flaminia Pavone, Roberto Coccurello
Summary: This study identified gender differences in immune responses to neuropathic pain, with females showing higher infiltration of T cells in nerves and males showing higher infiltration of macrophages. Additionally, cytokine and chemokine levels were differently regulated in blood and nerve lysates from female mice compared to male mice. These findings provide insights into gender-associated inflammatory profiles in neuropathy and could contribute to the development of differential biomarkers and personalized medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Hadil M. Alahdal, Fuad Ameen, Sami AlYahya, Hana Sonbol, Anas Khan, Yousef Alsofayan, Ahmed Alahmari
Summary: This study investigated the spread of COVID-19 through wastewater and the efficiency of sewage treatment in removing the virus. The results showed that sewage treatment was effective in removing SARS-CoV-2 viral RNA. However, the viral RNA load in wastewater was relatively low, indicating that sewage may only preserve viral RNA for a short time in a hot climate. Additionally, there was no significant correlation between hospital admissions and viral RNA in sewage.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Nicola Jeffery, David Chambers, Brandon M. Invergo, Ryan M. Ames, Lorna W. Harries
Summary: Inducing a somatostatin-positive cell population and characterizing its transcriptome revealed a key role of the HNRNPD gene in determining beta cell identity in response to cellular stress. These findings provide new insights into the role of RNA binding proteins and RNA biology in cell identity determination.
CELL AND BIOSCIENCE
(2021)
Editorial Material
Neurosciences
Karli Montague-Cardoso
NATURE NEUROSCIENCE
(2022)
Article
Infectious Diseases
Francesca Tortora, Luigi Dei Giudici, Raffaele Simeoli, Fabrizio Chiusolo, Sara Cairoli, Paola Bernaschi, Roberto Bianchi, Sergio Giuseppe Picardo, Carlo Dionisi Vici, Bianca Maria Goffredo
Summary: Fungal infections after liver transplantation are a serious complication. This study evaluated the levels of amphotericin-B in the peritoneum following intravenous administration, and found that the peritoneal levels were significantly lower than plasma and did not reach the therapeutic target.
Article
Multidisciplinary Sciences
Alia Aldahlawi, Afnan Alqadiri, Hadil Alahdal, Kalthoom Al-Sakkaf, Jehan Alrahimi, Fatemah Basingab
Summary: This study investigates the effects of TNF-alpha on the expression of TIM-3 and its ligand Gal-9 on DCs. The results show that TNF-alpha and LPS, either alone or in combination, can induce the maturation of DCs in vitro. However, the absence of both TNF-alpha and LPS leads to an immature state of DCs and induces the expression of TIM-3.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Reham Samir Hamida, Mohamed Abdelaal Ali, Zakiah Nasser Almohawes, Hadil Alahdal, Maha Abdullah Momenah, Mashael Mohammed Bin-Meferij
Summary: This study discovered a novel microalgal strain (BA_Chlo4) capable of synthesizing silver nanoparticles (C@Ag-NPs), which exhibited anticancer, antioxidant, and antibacterial activities.
Article
Pharmacology & Pharmacy
Alessandro Ferretti, Raffaele Simeoli, Sara Cairoli, Nicola Pietrafusa, Marina Trivisano, Carlo Dionisi Vici, Nicola Specchio, Bianca Maria Goffredo
Summary: This article reports the application of TDM in pediatric patients carrying KCNT1 genetic variants and treated with QND for DEE. The results suggest that QND may be a promising drug for these patients, despite lower blood levels than the therapeutic range. The study highlights the importance of TDM in monitoring drug toxic effects and evaluating drug-drug interactions.
Review
Infectious Diseases
Raffaele Simeoli, Sara Cairoli, Nunzia Decembrino, Francesca Campi, Carlo Dionisi Vici, Alberto Corona, Bianca Maria Goffredo
Summary: Due to complex maturational and physiological changes, neonatal pharmacology is different from children and adults. Antibiotics are widely used among preterms, but most of them are off-label due to the lack of clinical studies in this population.
Article
Chemistry, Analytical
Raffaele Simeoli, Sara Cairoli, Federica Galaverna, Marco Becilli, Emilia Boccieri, Giacomo Antonetti, Alessia Vitale, Alessandro Mancini, Chiara Rossi, Carlo Dionisi Vici, Bianca Maria Goffredo
Summary: This study demonstrates that VAMS and DPS can be used for therapeutic drug monitoring (TDM) of antifungal agents. The stability of these sampling devices allows for easy storage and shipping without refrigeration, making them suitable for remote TDM applications.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Infectious Diseases
Gabriella Bottari, Bianca Maria Goffredo, Marco Marano, Cristina Maccarrone, Raffaele Simeoli, Giuseppe Bianco, Leonardo Vallesi, Joseph Charles Charlie Beetham, Anna Teresa Mazzeo, Andrea Cappoli, Sara Cairoli, Raffaella Labbadia, Corrado Cecchetti, Paola Bernaschi, Tiziana Corsetti, Santo Morabito, Fabio Silvio Taccone, Isabella Guzzo
Summary: Extracorporeal therapies (ET) are increasingly used in pediatric settings as adjuvant therapeutic strategies for overwhelming inflammatory conditions. This prospective observational study demonstrated the different impact of CytoSorb (CS) and continuous kidney replacement therapy (CKRT) on the removal of antimicrobial drugs in critically ill children with sepsis/septic shock.
Article
Biochemistry & Molecular Biology
Licia Lugli, Elisabetta Garetti, Bianca Maria Goffredo, Francesco Candia, Sara Crestani, Caterina Spada, Isotta Guidotti, Luca Bedetti, Francesca Miselli, Elisa Muttini Della Casa, Maria Federica Roversi, Raffaele Simeoli, Sara Cairoli, Daniele Merazzi, Paola Lago, Lorenzo Iughetti, Alberto Berardi
Summary: This study aims to evaluate the pharmacokinetic characteristics of fentanyl in full-term newborns with hypoxic-ischemic encephalopathy (HIE) who have undergone therapeutic hypothermia (TH). By measuring fentanyl plasma concentrations, the study aims to optimize the dosing regimen of fentanyl during TH.
Article
Chemistry, Medicinal
Nada El-Megiri, Yasser M. Mostafa, Amal Ahmed, Eman T. Mehanna, Mona F. El-Azab, Fatma Alshehri, Hadil Alahdal, Norhan M. El-Sayed
Summary: The present study aimed to investigate the potential neuroprotective effect of Pioglitazone in PTZ kindled seizure in mice. Treatment with Pioglitazone demonstrated a significant decrease in NGF, seizure score, m-TOR, GLUT-1 and GLUT-3 in comparison to the PTZ group, suggesting that Pioglitazone provides its neuroprotective effect through modulating m-TOR signaling, glucose metabolism and NGF levels.
Article
Food Science & Technology
Wafa Abdullah Al-Megrin, Hany Mohamed Yehia, Shereen Magdy Korany, Mariam Abdulaziz Alkhateeb, Hadil Alahdal, Hana Sonbol, Afrah Fahd Alkhuriji, Manal Fawzy Elkhadragy
Summary: Probiotics have significant antibacterial activity against Campylobacter infection and can reduce inflammation and protect the intestinal structure.
FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Hany Mohamed Yehia, Manal Ahmed Gasmelseed Awad, Manal Fawzy Elkhadragy, Mariam Abdulaziz Alkhateeb, Hadil Alahdal, Hatem Salama Mohamed Ali, Reem Atta Alajami, Mohamed Ferkry Serag El-Din
Summary: A new method was developed to create nanoparticles from freeze-and air-dried mangosteen peel, and the effects of these particles on the growth and protein formation of various gram-positive foodborne pathogenic bacteria were studied. The results showed that the freeze-dried nanoparticles had higher antioxidant activity compared to the air-dried ones, and they could inhibit the protein formation of bacteria.
FOOD SCIENCE AND TECHNOLOGY
(2022)