Review
Pharmacology & Pharmacy
Chenghui Liang, Robert Flaumenhaft, Cai Yuan, Mingdong Huang
Summary: This article describes the structures and functions of vascular thiol isomerases (VTIs), summarizes the relationship between these enzymes and thrombosis, and focuses on the development of VTI inhibitors for antithrombotic applications.
DRUG DISCOVERY TODAY
(2022)
Article
Engineering, Biomedical
Ying Yuan, Shanchao Diao, Dong Zhang, Wanrong Yi, Baiwen Qi, Xiang Hu, Chen Xie, Quli Fan, Aixi Yu
Summary: A new nano-probe targeting thrombosis with activated near infrared II fluorescence has been developed using protein disulfide isomerase (PDI) as an activator. This probe demonstrates excellent photophysical stability, efficient fibrin targeting, and a high signal-to-background ratio, providing a visualized diagnosis and monitoring for thrombolysis in blood clot treatment.
MATERIALS TODAY BIO
(2023)
Article
Cell Biology
Philip John Robinson, Marie Anne Pringle, Bethany Fleming, Neil John Bulleid
Summary: Proteins in the secretory pathway require correct disulfide pairings for proper folding. Two important members of the protein disulfide isomerase (PDI) family, ERp57 and ERdj5, have unclear roles in disulfide formation, but ERp57 is involved in the isomerisation of non-native disulfides in glycoproteins. Alternative PDIs can compensate for the absence of ERp57 in isomerising glycoprotein disulfides, but only when a robust reductive pathway is present. ERdj5 is required for this alternative pathway as a reductase. These findings clarify the essential functions of two PDIs in the cell and highlight the distinctions between formation, reduction, and isomerisation of disulfide bonds.
JOURNAL OF CELL SCIENCE
(2023)
Review
Oncology
Ke Zhang, Yuan Li, Xiangyi Kong, Chuqi Lei, Huaiyu Yang, Nianchang Wang, Zhongzhao Wang, Hu Chang, Lixue Xuan
Summary: AGR2 is a secreted protein widely found in breast tissue. Its increased expression in precancerous lesions, primary tumors, and metastatic tumors raises interest. This review discusses the gene and protein structure of AGR2, highlighting its various functions within and outside breast cancer cells due to its endoplasmic reticulum retention sequence, protein disulfide isomerase active site, and multiple protein binding sequences. The review also explores the role of AGR2 in breast cancer progression and prognosis, emphasizing its potential as a biomarker and target for immunotherapy, providing new insights for early diagnosis and treatment of breast cancer.
FRONTIERS IN ONCOLOGY
(2023)
Article
Medicine, Research & Experimental
Ruili Guo, Xue Wang, Yani Fang, Xiongjian Chen, Kun Chen, Wenting Huang, Jun Chen, Jian Hu, Fei Liang, Jingting Du, Confidence Dordoe, Xianxi Tian, Li Lin
Summary: In this study, it was found that rhFGF20 can reduce brain edema, Evans blue penetration, and upregulate the expression of BBB-related tight junction proteins after TBI. rhFGF20 promotes angiogenesis and contributes to neurological and cognitive function recovery in the TBI repair phase. Through the Wnt/beta-catenin pathway, rhFGF20 may prevent vascular repair and angiogenesis.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Medicine, General & Internal
Hongtao Lan, Zhoujie Tong, Yaqiong Jiao, Haitao Han, Ying Ma, Yulin Li, Xu Jia, Boang Hu, Wei Zhang, Ming Zhong, Zhihao Wang
Summary: This study reveals that endothelial-derived extracellular vesicles (EVs) carrying protein disulfide isomerase (PDI) play a crucial role in platelet-dependent deep vein thrombosis (DVT) development. PDI interacts with glucose-regulated protein 94 (GRP94), leading to the activation of platelets and increased expression of GPIIb/IIIa. PDI may be a potential target for DVT prevention.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Immunology
Jing Li, Tripti Kumari, Andrew Barazia, Vishwanath Jha, Si-Yeon Jeong, Amber Olson, Mijeong Kim, Bum-Kyu Lee, Vijayprakash Manickam, Zhimin Song, Regina Clemens, Babak Razani, Jonghwan Kim, Mary C. Dinauer, Jaehyung Cho
Summary: The interaction between neutrophils and endothelial cells plays a critical role in the development of vascular inflammation. This study uncovers the regulatory mechanism of neutrophil adhesive function, highlighting the role of neutrophil DREAM in promoting neutrophil recruitment in inflammation-related conditions.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Denise C. Fernandes, Joao Wosniak Jr, Renata C. Goncalves, Leonardo Y. Tanaka, Carolina G. Fernandes, Daniela B. Zanatta, Ana Barbosa M. de Mattos, Bryan E. Strauss, Francisco R. M. Laurindo
Summary: PDIA1 regulates the balance between NOX1 and NOX4, influencing VSMC differentiation and playing a significant role in vascular smooth muscle cell phenotype.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Hematology
Angelina J. Lay, Alexander Dupuy, Lejla Hagimola, Jessica Tieng, Mark Larance, Yunwei Zhang, Jean Yang, Yvonne Kong, Joyce Chiu, Emilia Gray, Zihao Qin, Diana Schmidt, Jessica Maclean, Benjamin Hofma, Marc Ellis, Maggie Kalev-Zylinska, Yair Argon, Shaun P. Jackson, Philip Hogg, Freda H. Passam
Summary: This study found that ERp5 plays a negative regulatory role in platelet ER stress response and highlights the importance of disulfide isomerases in platelet ER homeostasis. Furthermore, the study also revealed the close correlation between platelet ER stress and platelet secretion and thrombosis.
Article
Multidisciplinary Sciences
Ju-Sim Kim, Alexandra Born, James Karl A. Till, Lin Liu, Sashi Kant, Morkos A. Henen, Beat Vogeli, Andres Vazquez-Torres
Summary: The specific interaction between a sensor kinase and its response regulator ensures accurate partner selectivity in bacteria. The study reveals that conserved residues on the surface of thioredoxin play a crucial role in the interactions with structurally diverse response regulators.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Tobias Ocklenburg, Fabian Neumann, Alexandra Wolf, Julia Vogel, Kirsten Goepelt, Melanie Baumann, Jennifer Baumann, Philip Kranz, Eric Metzen, Ulf Brockmeier
Summary: ERp57, a disulfide isomerase originally found in the endoplasmic reticulum, has been suggested as a potential target for cancer therapy due to its critical role in tumor cell proliferation. Knocking down ERp57 under hypoxia was shown to inhibit cell growth and enhance radiosensitivity in colorectal cancer cells, suggesting its potential as a therapeutic target in cancer treatment. However, PDPK1 was not favored as a suitable pharmaceutical target due to its lack of inhibitory effect on proliferation when efficiently knocked down or chemically inhibited.
SCIENTIFIC REPORTS
(2021)
Review
Cell Biology
Simona Del Giudice, Valentina De Luca, Seyedehnegar Parizadeh, Domenico Russo, Alberto Luini, Rosaria Di Martino
Summary: This article reviews the autoregulatory circuits acting on the Golgi complex, with a focus on the role of specific signaling molecules in cancer. It proposes to draw awareness to the Golgi-localized regulatory systems as potential targets in cancer therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Ji Hu, Ru Chen, Jie An, Yilong Wang, Minglu Liang, Kai Huang
Summary: The study demonstrates that dauricine can alleviate endothelial inflammation by suppressing the NF-κB pathway, leading to a decrease in adhesion of THP-1 cells to HUVECs and protection against acute lung injury in mice.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Cell Biology
Jaleh S. Mesgarzadeh, Joel N. Buxbaum, R. Luke Wiseman
Summary: Genetic, environmental, and aging-related insults can lead to misfolding and aggregation of secreted proteins involved in various diseases. Understanding the molecular mechanisms of extracellular proteostasis is important for protecting the secreted proteome from pathological insults.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Immunology
Laura Chavez, Julia Meguro, Shaoyi Chen, Vanessa Nunes de Paiva, Ronald Zambrano, Julia M. Eterno, Rahul Kumar, Matthew R. Duncan, Merline Benny, Karen C. Young, W. Dalton Dietrich, Roberta Brambilla, Shu Wu, Augusto F. Schmidt
Summary: Mechanical ventilation in preterm newborns can cause lung and brain inflammation, as well as activate pyroptosis pathways. Circulating extracellular vesicles (EVs) isolated from neonatal rats with ventilation-induced lung injury can lead to neuroinflammation and activation of caspase-1 and gasdermin D in the brain, mimicking the effects of mechanical ventilation.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Biochemistry & Molecular Biology
James Schiemer, Andrew Bohm, Li Lin, Glenn Merrill-Skoloff, Robert Flaumenhaft, Jin-Sheng Huang, Guy C. Le Breton, Athar H. Chishti
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Hematology
Jaehyung Cho, Daniel R. Kennedy, Lin Lin, Mingdong Huang, Glenn Merrill-Skoloff, Barbara C. Furie, Bruce Furie
Article
Hematology
Valerie Proulle, Richard A. Furie, Glenn Merrill-Skoloff, Barbara C. Furie, Bruce Furie
Article
Hematology
Alexander Y. Mitrophanov, Glenn Merrill-Skoloff, Steven P. Grover, Vijay Govindarajan, Arun Kolanjiyil, Daniel S. Hariprasad, Ginu Unnikrishnan, Robert Flaumenhaft, Jaques Reifman
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2020)
Article
Medicine, Research & Experimental
Sol Schulman, Emale El-Darzi, Mary H. C. Florido, Max Friesen, Glenn Merrill-Skoloff, Marisa A. Brake, Calvin R. Schuster, Lin Lin, Randal J. Westrick, Chad A. Cowan, Robert Flaumenhaft, Nihr BioResource, Willem H. Ouwehand, Kathelijne Peerlinck, Kathleen Freson, Ernest Turro, Bruce Furie
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Hematology
Joseph E. Italiano, Markus Bender, Glenn Merrill-Skoloff, Cedric Ghevaert, Bernhard Nieswandt, Robert Flaumenhaft
Article
Cardiac & Cardiovascular Systems
Anna Gromotowicz-Poplawska, Robert Flaumenhaft, Shadi K. Gholami, Glenn Merrill-Skoloff, Ewa Chabielska, Gordon H. Williams, Jose R. Romero
Summary: The deficiency of striatin is associated with an increased prothrombotic response, demonstrating its protective role in experimental thrombosis. This protective effect may be reduced in human striatin risk allele carriers, indicating potential implications in blood pressure regulation.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Hematology
Steven P. Grover, Pavan K. Bendapudi, Moua Yang, Glenn Merrill-Skoloff, Vijay Govindarajan, Alexander Y. Mitrophanov, Robert Flaumenhaft
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2020)
Article
Immunology
Nari Harakawa, Akiko Shigeta, Masahiro Wato, Glenn Merrill-Skoloff, Barbara C. Furie, Bruce Furie, Toshiro Okazaki, Naochika Domae, Masayuki Miyasaka, Takako Hirata
INTERNATIONAL IMMUNOLOGY
(2007)
Article
Hematology
Christophe Dubois, Laurence Panicot-Dubois, Glenn Merrill-Skoloff, Bruce Furie, Barbara C. Furie
Article
Hematology
S Falati, S Patil, PL Gross, M Stapleton, G Merrill-Skoloff, NE Barrett, KL Pixton, H Weiler, B Cooley, DK Newman, PJ Newman, BC Furie, B Furie, JM Gibbins
Article
Cell Biology
PL Gross, BC Furie, G Merrill-Skoloff, J Chou, B Furie
JOURNAL OF LEUKOCYTE BIOLOGY
(2005)
Article
Hematology
J Chou, N Mackman, G Merrill-Skoloff, B Pedersen, BC Furie, B Furie
Article
Immunology
S Falati, QD Liu, P Gross, G Merrill-Skoloff, J Chou, E Vandendries, A Celi, K Croce, BC Furie, B Furie
JOURNAL OF EXPERIMENTAL MEDICINE
(2003)