4.8 Article

A cdk1 gradient guides surface contraction waves in oocytes

期刊

NATURE COMMUNICATIONS
卷 8, 期 -, 页码 -

出版社

NATURE PORTFOLIO
DOI: 10.1038/s41467-017-00979-6

关键词

-

资金

  1. European Molecular Biology Laboratory
  2. EMBL International PhD Programme

向作者/读者索取更多资源

Surface contraction waves (SCWs) in oocytes and embryos lead to large-scale shape changes coupled to cell cycle transitions and are spatially coordinated with the cell axis. Here, we show that SCWs in the starfish oocyte are generated by a traveling band of myosin IIdriven cortical contractility. At the front of the band, contractility is activated by removal of cdk1 inhibition of the RhoA/RhoA kinase/myosin II signaling module, while at the rear, contractility is switched off by negative feedback originating downstream of RhoA kinase. The SCW's directionality and speed are controlled by a spatiotemporal gradient of cdk1-cyclinB. This gradient is formed by the release of cdk1-cyclinB from the asymmetrically located nucleus, and progressive degradation of cyclinB. By combining quantitative imaging, biochemical and mechanical perturbations with mathematical modeling, we demonstrate that the SCWs result from the spatiotemporal integration of two conserved regulatory modules, cdk1-cyclinB for cell cycle regulation and RhoA/Rok/NMYII for actomyosin contractility.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Article Cell Biology

Single-oocyte transcriptome analysis reveals aging-associated effects influenced by life stage and calorie restriction

Tappei Mishina, Namine Tabata, Tetsutaro Hayashi, Mika Yoshimura, Mana Umeda, Masashi Mori, Yayoi Ikawa, Hiroshi Hamada, Itoshi Nikaido, Tomoya S. Kitajima

Summary: Chromosome segregation errors in oocytes due to aging can lead to the production of aneuploid eggs, with calorie restriction showing potential to prevent this age-related issue. Transcriptome changes are observed in oocytes at different reproductive stages, with up-regulation of genes involved in chromosome segregation under calorie restriction.

AGING CELL (2021)

Article Biochemistry & Molecular Biology

KAHRP dynamically relocalizes to remodeled actin junctions and associates with knob spirals in Plasmodium falciparum-infected erythrocytes

Cecilia P. Sanchez, Pintu Patra, Shih-Ying Scott Chang, Christos Karathanasis, Lukas Hanebutte, Nicole Kilian, Marek Cyrklaff, Mike Heilemann, Ulrich S. Schwarz, Mikhail Kudryashev, Michael Lanzer

Summary: KAHRP plays a key role in Plasmodium falciparum malaria by forming membrane protrusions in infected erythrocytes, anchoring parasite-encoded adhesins to the membrane skeleton. Through super-resolution microscopy, it was found that KAHRP initially associates with various skeletal components before eventually colocalizing with remnant actin junctions under the spiral scaffold forming knobs. Additionally, a dynamic model of KAHRP organization and its function in attaching other factors to the spiral scaffold was proposed based on the findings.

MOLECULAR MICROBIOLOGY (2022)

Article Biophysics

Quantifying force transmission through fibroblasts: changes of traction forces under external shearing

Steven Huth, Johannes W. Blumberg, Dimitri Probst, Jan Lammerding, Ulrich S. Schwarz, Christine Selhuber-Unkel

Summary: Researchers have developed a novel approach to quantify intracellular force transmission by combining microneedle shearing and traction force microscopy. The experiment shows that cells dynamically redistribute forces under external shearing and experience changes in force loading during sequential rupture of their adhesion sites. This strategy offers new perspectives for future studies of force transmission and mechanotransduction in cells.

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS (2022)

Article Multidisciplinary Sciences

Comparison of direct and inverse methods for 2.5D traction force microscopy

Johannes W. Blumberg, Ulrich S. Schwarz

Summary: The text discusses a systematic comparison between two fundamentally different approaches to 2.5D traction force microscopy (TFM). The direct method involves calculating strain and stress tensors directly from displacement data, while the inverse method minimizes the difference between estimated and measured displacements. Experimental results show that the direct method approaches the performance of 2.5D FTTC for larger noise and does not necessarily require a divergence correction. Additionally, increasing resolution benefits the direct method more than the inverse method.

PLOS ONE (2022)

Article Physics, Multidisciplinary

Collective migration reveals mechanical flexibility of malaria parasites

Pintu Patra, Konrad Beyer, Astha Jaiswal, Anna Battista, Karl Rohr, Friedrich Frischknecht, Ulrich S. Schwarz

Summary: The collective motion of malaria parasites, specifically the Plasmodium sporozoites, is analyzed. It is found that the mechanical flexibility of the sporozoites is favorable for transmission, as it allows for sorting of the parasites based on their curvatures and speeds. Additionally, the vortices formed by the sporozoites exhibit oscillatory breathing due to the storage of motility force in their elastic energy.

NATURE PHYSICS (2022)

Article Biochemical Research Methods

A particle-based computational model to analyse remodelling of the red blood cell cytoskeleton during malaria infections

Julia Jaeger, Pintu Patra, Cecilia P. Sanchez, Michael Lanzer, Ulrich S. Schwarz

Summary: Malaria is a deadly infectious disease caused by a parasite that multiplies within red blood cells. Researchers have developed a computational model to predict the changes in mechanical properties and protein distribution in infected red blood cells. Their simulations show that specific proteins can relocate on the red blood cell surface due to changes in binding affinities, in agreement with experimental observations. This model can provide further insights into the mechanism of malaria parasite attack on red blood cell cytoskeleton.

PLOS COMPUTATIONAL BIOLOGY (2022)

Article Physics, Multidisciplinary

First-passage times in complex energy landscapes: a case study with nonmuscle myosin II assembly

Rick Bebon, Ulrich S. Schwarz

Summary: The article explores the computation of complex energy landscapes in biological systems, particularly their effects on first-passage times (FPTs). The authors propose a method to identify the most relevant features of energy landscapes by coarse-graining the Fokker-Planck equation to a master equation and decomposing its FPTs iteratively. They apply this method to the electrostatic interaction between two nonmuscle myosin II (NM2) rods and identify the most relevant energy barriers for their self-assembly into NM2 minifilaments and how they change under force.

NEW JOURNAL OF PHYSICS (2022)

Article Biophysics

Cell size and actin architecture determine force generation in optogenetically activated cells

T. Andersen, D. Woerthmueller, D. Probst, I. Wang, P. Moreau, V. Fitzpatrick, T. Boudou, U. S. Schwarz, M. Balland

Summary: Adherent cells generate mechanical force and sense the physical properties of their environment using actomyosin contractility, which has important implications for cell migration, division, differentiation, and fate. The organization of the actomyosin system within cells is highly variable and controlled by small GTPases from the Rho family. Activation of Rho regulators leads to cell-scale force generation, and the dynamics of this response are influenced by cell size and the architecture of the actin cytoskeleton, which are in turn regulated by the extracellular environment.

BIOPHYSICAL JOURNAL (2023)

Article Cell Biology

Clathrin coats partially preassemble and subsequently bend during endocytosis

Markus Mund, Aline Tschanz, Yu-Le Wu, Felix Frey, Johanna L. Mehl, Marko Kaksonen, Ori Avinoam, Ulrich S. Schwarz, Jonas Ries

Summary: The study reveals the three-dimensional shapes of clathrin coats during endocytosis and introduces a novel Cooperative Curvature Model that accurately describes the changes in shapes and dynamics. The findings provide insights into the mechanism of clathrin coat remodeling during endocytosis and offer a potential general model for clathrin coat remodeling on the plasma membrane.

JOURNAL OF CELL BIOLOGY (2023)

Article Biophysics

High curvature promotes fusion of lipid membranes: Predictions from continuum elastic theory

Gonen Golani, Ulrich S. Schwarz

Summary: The fusion of lipid membranes involves significant energy barriers related to stalk and fusion pore formation. By using continuum elastic theory, the relationship between membrane shape and energy barriers was determined. The stalk formation energy decreases with curvature, while the fusion pore formation energy barrier shows a more complicated behavior. These findings can be utilized to prevent viral infections and inhibit polymorphic virus infection.

BIOPHYSICAL JOURNAL (2023)

Review Cell Biology

Building a FAIR image data ecosystem for microscopy communities

Isabel Kemmer, Antje Keppler, Beatriz Serrano-Solano, Arina Rybina, Bugra Oezdemir, Johanna Bischof, Ayoub El Ghadraoui, John E. Eriksson, Aastha Mathur

Summary: Bioimaging is facing challenges in handling, analyzing, and managing the increasingly complex datasets due to the rapid development of microscopy technologies. Efforts and solutions are being developed by the microscopy community to address these challenges, and collaborations within the microscopy ecosystem, as well as research infrastructures like Euro-BioImaging, are shaping the field.

HISTOCHEMISTRY AND CELL BIOLOGY (2023)

Article Chemistry, Physical

Grand canonical Brownian dynamics simulations of adsorption and self-assembly of SAS-6 rings on a surface

Santiago Gomez Melo, Dennis Woerthmueller, Pierre Gonczy, Niccolo Banterle, Ulrich S. Schwarz

Summary: SAS-6 self-assembles into rings on a surface, ensuring the nine-fold symmetry of centriole organelle. Simulation and experimental comparison show that weak interaction energies and small angular range are necessary for the selection of nine-fold symmetry.

JOURNAL OF CHEMICAL PHYSICS (2023)

Article Chemistry, Multidisciplinary

Membrane Tension Inhibits Lipid Mixing by Increasing the Hemifusion Stalk Energy

Petr Shendrik, Gonen Golani, Raviv Dharan, Ulrich S. Schwarz, Raya Sorkin

Summary: Fusion of biological membranes plays a fundamental role in various physiological events. The energy barriers of fusion stages are tightly dependent on the mechanical and physical properties of the system, including membrane tension. This study reveals that membrane tension inhibits lipid mixing and increases the energy barrier of hemifusion stalk formation, leading to a delay in lipid mixing time.

ACS NANO (2023)

Review Biochemical Research Methods

Community-developed checklists for publishing images and image analyses

Christopher Schmied, Michael S. Nelson, Sergiy Avilov, Gert-Jan Bakker, Cristina Bertocchi, Johanna Bischof, Ulrike Boehm, Jan Brocher, Mariana T. Carvalho, Catalin Chiritescu, Jana Christopher, Beth A. Cimini, Eduardo Conde-Sousa, Michael Ebner, Rupert Ecker, Kevin Eliceiri, Julia Fernandez-Rodriguez, Nathalie Gaudreault, Laurent Gelman, David Grunwald, Tingting Gu, Nadia Halidi, Mathias Hammer, Matthew Hartley, Marie Held, Florian Jug, Varun Kapoor, Ayse Aslihan Koksoy, Judith Lacoste, Sylvia Le Devedec, Sylvie Le Guyader, Penghuan Liu, Gabriel G. Martins, Aastha Mathur, Kota Miura, Paula Montero Llopis, Roland Nitschke, Alison North, Adam C. Parslow, Alex Payne-Dwyer, Laure Plantard, Rizwan Ali, Britta Schroth-Diez, Lucas Schuetz, Ryan T. Scott, Arne Seitz, Olaf Selchow, Ved P. Sharma, Martin Spitaler, Sathya Srinivasan, Caterina Strambio-De-Castillia, Douglas Taatjes, Christian Tischer, Helena Klara Jambor

Summary: This article presents community-developed checklists for preparing light microscopy images and describing image analyses in order to enhance the clarity and reproducibility of image figures and improve the quality and explanatory power of microscopy data.

NATURE METHODS (2023)

Article Physics, Multidisciplinary

Optogenetic control of migration of contractile cells predicted by an active gel model

Oliver M. Drozdowski, Falko Ziebert, Ulrich S. Schwarz

Summary: Cell crawling on flat substrates is driven by the interplay between actin polymerization at the front and myosin contractility at the back. Optogenetics provides a way to experimentally control contraction and cell migration. Theoretical analysis using a one-dimensional active gel model predicts the possibility of switching between sessile and motile states through optogenetic activation or inhibition of contractility.

COMMUNICATIONS PHYSICS (2023)

暂无数据