Article
Biochemistry & Molecular Biology
Azamat V. Karginov, Marina G. Tarutina, Anastasia R. Lapteva, Maria D. Pakhomova, Artur A. Galliamov, Sergey Y. Filkin, Alexey N. Fedorov, Michael O. Agaphonov
Summary: In this work, an improved plasmid vector set for CRISPR-Cas9 genome editing in methylotrophic yeasts is presented. The vector set includes a plasmid encoding Cas9 with a nuclear localization signal and plasmids with a scaffold for the single guide RNA (sgRNA). Construction of sgRNA gene for a specific target sequence only requires the insertion of a 24 bp oligonucleotide duplex into the scaffold. The functionality of this vector set has been demonstrated in multiple methylotrophic yeast species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Stefan J. Tekel, Nicholas Brookhouser, Kylie Standage-Beier, Xiao Wang, David A. Brafman
Summary: The introduction of transient reporters of editing enrichment (TREE) has enabled highly efficient single-base editing of human cells using a transient episomal fluorescent reporter, allowing for rapid generation of clonal editing efficiencies exceeding 80% in biallelic or multiplexed edited isogenic human pluripotent stem cell lines within approximately 3-4 weeks.
Review
Engineering, Biomedical
Chao Li, Yawei Du, Tongtong Zhang, Haoran Wang, Zhiyong Hou, Yingze Zhang, Wenguo Cui, Wei Chen
Summary: CRISPR/Cas9 is a revolutionary genome editing technology with numerous advantages and great potential in bone and cartilage repair. This review discusses the research status and development trends of CRISPR/Cas9 in bone and cartilage repair.
BIOACTIVE MATERIALS
(2023)
Review
Agriculture, Dairy & Animal Science
Julia Popova, Victoria Bets, Elena Kozhevnikova
Summary: Genome editing has practical applications in farm animals, improving production traits, economic value, and disease resistance. It also has potential in biomedical research and drug production, as well as xenograft donors. Recent advancements in site-specific nucleases and embryological delivery methods have revolutionized transgenesis, providing efficient and reliable tools for genome engineering in agriculture.
Review
Biochemistry & Molecular Biology
Yulin Mu, Chengxiao Zhang, Taihua Li, Feng-Jie Jin, Yun-Ju Sung, Hee-Mock Oh, Hyung-Gwan Lee, Long Jin
Summary: Lactobacillus, important in food production and probiotics, can benefit from CRISPR/Cas9-based genome editing to improve strain efficiency and achieve traceless genome modification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biology
Alpana Joshi, Seo-Yeon Yang, Hyung-Geun Song, Jiho Min, Ji-Hoon Lee
Summary: Plants are affected by various environmental stresses, which have a negative impact on their growth and development and limit crop productivity. Developing cultivars resistant to abiotic stresses is essential to meet the needs of the growing world population and ensure food security. With the availability of genetic databases and advancements in genome editing techniques, it is now possible to edit target genes with precision and create new opportunities for crop improvement. The CRISPR-Cas system is a powerful genome editing method that allows the development of improved cultivars with resistance to abiotic stresses. This review discusses the current advances in understanding the molecular mechanisms of abiotic stress responses in plants and the application of CRISPR-Cas system genome editing technology for developing crop resilience against drought, salinity, temperature, heavy metals, and herbicides.
Review
Pharmacology & Pharmacy
Ning Ding, Sangsin Lee, Matan Lieber-Kotz, Jie Yang, Xue Gao
Summary: With over 466 million people worldwide affected by hearing loss, genetic factors contributing to 50% of congenital hearing loss, CRISPR-Cas9 systems offer new gene therapy strategies for treating genetic hearing impairment. This technology shows promise in targeted gene editing for deafness-associated genes and has the potential to revolutionize the treatment of genetic hearing diseases.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Neurosciences
Isabel Espinosa-Medina, Daniel Feliciano, Carla Belmonte-Mateos, Rosa Linda Miyares, Jorge Garcia-Marques, Benjamin Foster, Sarah Lindo, Cristina Pujades, Minoru Koyama, Tzumin Lee
Summary: During development, regulatory factors determine cell fates in a precise order. However, current strategies for tracing cell lineages lack access to sequentially produced cells. In this study, the authors present TEMPO, a genetic tool based on CRISPR that allows sequential labeling and manipulation of cell generations in vertebrates. TEMPO enables visualization and manipulation of molecular factors crucial for studying cell-type specification.
Review
Biochemistry & Molecular Biology
Sunny Ahmar, Paulina Ballesta, Mohsin Ali, Freddy Mora-Poblete
Summary: Forest tree breeding focuses on improving economic traits and resistance, with the assistance of genomics for better understanding genetic architecture. Challenges include accuracy of genomic prediction models, limited genotypes for gene editing, and lack of transformation methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Xueli Zhang, Chaohui Zhang, Caijiao Liang, Bizhou Li, Fanmei Meng, Yuncan Ai
Summary: Bacteriophages, the most abundant organisms in the biosphere, have been sequenced extensively. However, the study of bacteriophage functional genomics has been hindered by a lack of effective research methods. This study designed a phage genome editing platform based on the CRISPR-Cas9 system, and successfully achieved gene editing in Vibrio natriegens phage TT4P2. This platform has the potential to advance research on phage gene diversity and accelerate the development of phage synthetic biology and nanotechnology.
MICROBIOLOGY SPECTRUM
(2022)
Review
Agronomy
Rukmini Mishra, Wei Zheng, Raj Kumar Joshi, Zhao Kaijun
Summary: Emerging pests and phytopathogens have threatened global food security by reducing crop yield and quality. Although traditional breeding methods and genetically modified crops have played a crucial role, their limitations have led to increasing acceptance of genome editing tools like CRISPR/Cas9 for crop improvement. This review discusses the potential of genome editing in enhancing disease resistance in rice and highlights the efficiency, flexibility, and adaptability of the CRISPR/Cas9 system.
Review
Pharmacology & Pharmacy
Siwei Chen, Deng Chen, Bin Liu, Hidde J. Haisma
Summary: CRISPR/Cas9-mediated genome engineering has extensive applications in basic biology, biotechnology, and medicine. However, the low gene modification efficiency and uncontrollable prolonged Cas9 activity hinder its further use. Researchers have explored small molecules with clinical potential to precisely modulate CRISPR/Cas9 genome editing activity.
DRUG DISCOVERY TODAY
(2022)
Review
Biochemistry & Molecular Biology
Min Ho Lee, Jae Il Shin, Jae Won Yang, Keum Hwa Lee, Do Hyeon Cha, Jun Beom Hong, Yeoeun Park, Eugene Choi, Kalthoum Tizaoui, Ai Koyanagi, Louis Jacob, Seoyeon Park, Ji Hong Kim, Lee Smith
Summary: This review highlights the current and potential use of CRISPR-Cas9 in the management of autoimmune diseases. Several studies have shown that CRISPR-Cas9 can be utilized for immunomodulation, reducing cholesterol, and treating rare diseases. However, there is limited research on the treatment of autoimmune diseases using CRISPR-Cas9.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Kristina J. Tatiossian, Robert D. E. Clark, Chun Huang, Matthew E. Thornton, Brendan H. Grubbs, Paula M. Cannon
Summary: This study found that CRISPR-Cas9 indel signatures can be used to identify gRNAs that maximize HDR outcomes, with the frequency of deletions resulting from MMEJ repair of at least 3 base pairs better predicts HDR frequency. Existing tools can be repurposed to identify gRNAs that promote HDR.
Article
Plant Sciences
Syed Inzimam Ul Haq, Dianfeng Zheng, Naijie Feng, Xingyu Jiang, Feng Qiao, Jin-Sheng He, Quan-Sheng Qiu
Summary: This review summarizes the advances of CRISPR/Cas9 technology in plant genome editing and discusses its applications in forage breeding, as well as the potential and challenges.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hanbo Li, Baofeng Su, Guyu Qin, Zhi Ye, Ahmed Elaswad, Ahmed Alsaqufi, Dayan A. Perera, Zhenkui Qin, Ramji Odin, Khoi Vo, David Drescher, Dalton Robinson, Sheng Dong, Dan Zhang, Mei Shang, Nermeen Abass, Sanjay K. Das, Max Bangs, Rex A. Dunham
MARINE BIOTECHNOLOGY
(2018)
Article
Biology
Lisui Bao, Changxu Tian, Shikai Liu, Yu Zhang, Ahmed Elaswad, Zihao Yuan, Karim Khalil, Fanyue Sun, Yujia Yang, Tao Zhou, Ning Li, Suxu Tan, Qifan Zeng, Yang Liu, Yueru Li, Yun Li, Dongya Gao, Rex Dunham, Kenneth Davis, Geoffrey Waldbieser, Zhanjiang Liu
Article
Fisheries
Ahmed Elaswad, Karim Khalil, Zhi Ye, Ahmed Alsaqufi, Hisham Abdelrahman, Baofeng Su, Dayan A. Perera, Sheng Dong, Nermeen Abass, Rex Dunham
NORTH AMERICAN JOURNAL OF AQUACULTURE
(2019)
Review
Medicine, General & Internal
Yosra A. Helmy, Mohamed Fawzy, Ahmed Elaswad, Ahmed Sobieh, Scott P. Kenney, Awad A. Shehata
JOURNAL OF CLINICAL MEDICINE
(2020)
Review
Medicine, Research & Experimental
Sina Salajegheh Tazerji, Phelipe Magalhaes Duarte, Parastoo Rahimi, Fatemeh Shahabinejad, Santosh Dhakal, Yashpal Singh Malik, Awad A. Shehata, Juan Lama, Jorn Klein, Muhammad Safdar, Md. Tanvir Rahman, Krzysztof J. Filipiak, Alfonso J. Rodriguez-Morales, Md. Abdus Sobur, Farrokhreza Kabir, Bita Vazir, Leonard Mboera, Marco Caporale, Md. Saiful Islam, John H. Amuasi, Rasha Gharieb, Paola Roncada, Sahar Musaad, Bruno Tilocca, Mohammad Kazem Koohi, Ali Taghipour, Ahmet Sait, Kannan Subbaram, Alireza Jahandideh, Pejman Mortazavi, Mohammad Amin Abedini, David A. Hokey, Unarose Hogan, Mohamed N. F. Shaheen, Ahmed Elaswad, Mahmoud M. Elhaig, Mohamed Fawzy
JOURNAL OF TRANSLATIONAL MEDICINE
(2020)
Article
Multidisciplinary Sciences
Ahmed Elaswad, Mohamed Fawzy, Shereen Basiouni, Awad A. Shehata
Article
Fisheries
Nermeen Y. Abass, Baofeng Su, Dayan A. Perera, Zhenkui Qin, Hanbo Li, Ahmed Alsaqufi, Ahmed Elaswad, Zhi Ye, Sheng Dong, Rex A. Dunham
Summary: GH cDNA constructs were transferred to channel catfish via electroporation, resulting in increased growth rate in F-1 individuals. Transgenic channel catfish exhibited larger body sizes compared to non-transgenic full-siblings. The family effect played a significant role in the growth response to the gene transfer.
Article
Biochemical Research Methods
Yingqi Huang, William Bugg, Max Bangs, Guyu Qin, David Drescher, Nathan Backenstose, Chia Chen Weng, Yiliu Zhang, Karim Khalil, Sheng Dong, Ahmed Elaswad, Zhi Ye, Cuiyu Lu, Khoi Vo, Rhoda Mae Simora, Xiaoli Ma, Zachary Taylor, Yujia Yang, Tao Zhou, Jingping Guo, Guillaume Salze, Zhenkui Qin, Yi Wang, Rex A. Dunham
Summary: Insertion of the beta actin-D5D transgene into channel catfish significantly increased the relative proportion of n-3 fatty acids in F1 transgenic fish, affecting growth, disease resistance, and other traits.
TRANSGENIC RESEARCH
(2021)
Article
Genetics & Heredity
Walaa A. Husseiny, Abeer A. Hassanin, Adel A. S. El Nabtiti, Karim Khalil, Ahmed Elaswad
Summary: The study investigated the effects of colloidal silver nanoparticles on myogenesis-related gene expression in chicken embryos. Results showed that low concentration (20 ppm) of Nano-Ag enhanced myogenesis by increasing the expression of MYOD1, MYF5, and MYF6 at the transcriptional level and MYOD1 at the translational level. Histological analysis also revealed hyperplasia in the treatment group injected with 20 ppm Nano-Ag, suggesting that in ovo injection of 20 ppm Nano-Ag promotes muscle development in chicken embryos compared to the 40 ppm dosage.
Article
Microbiology
Ahmed Elaswad, Mohamed Fawzy
Summary: As SARS-CoV-2 is detected in animals, mutations in the virus genome may lead to mismatches with diagnostic PCR assays, necessitating modifications to oligo sequences. This study identified mismatches in some PCR assays targeting different virus genes, potentially impacting the accuracy of test results.
Article
Biotechnology & Applied Microbiology
Nermeen Y. Abass, Baofeng Su, Ahmed Alsaqufi, Ahmed Elaswad, Zhenkui Qin, Hanbo Li, Ramjie Odin, Zhi Ye, Rex A. Dunham
Summary: This study compared the growth performance of female and male transgenic channel catfish at 16 and 48 months of age. Transgenic fish showed significantly larger body weight compared to non-transgenic siblings, with growth differences depending on age and sex. Sexually dimorphic responses to GH transgenes were observed, with higher survival rates in non-transgenic fish during oxygen depletion.
MARINE BIOTECHNOLOGY
(2021)
Article
Biology
Guyu Qin, Zhenkui Qin, Cuiyu Lu, Zhi Ye, Ahmed Elaswad, Max Bangs, Hanbo Li, Yiliu Zhang, Yingqi Huang, Huitong Shi, Kamal Gosh, Nermeen Y. Abass, Khoi Vo, Ramjie Odin, William S. Bugg, Nathan J. C. Backenstose, David Drescher, Zachary Taylor, Timothy Braden, Baofeng Su, Rex A. Dunham
Summary: Gonadotropin-releasing hormone (GnRH) is crucial for fish reproduction. By using TALEN to target the cfGnRH gene in channel catfish, gene editing was achieved to control fish reproduction. Application of LHRHa hormone therapy improved spawning and hatch rates for mutants, showing potential for reproductive control of gene-engineered fish.
Article
Fisheries
Michael Coogan, Veronica Alston, Baofeng Su, Karim Khalil, Ahmed Elaswad, Mohd Khan, Rhoda M. C. Simora, Andrew Johnson, De Xing, Shangjia Li, Jinhai Wang, Cuiyu Lu, Wenwen Wang, Darshika Hettiarachchi, Tasnuba Hasin, Jeffery Terhune, Ian A. E. Butts, Rex A. Dunham
Summary: This study investigated the effects of CRISPR/Cas9 knockout of the myostatin gene in channel catfish. The results showed that gene-edited channel catfish exhibited high mutation and inheritance rates, as well as improved growth and disease resistance compared to control groups. Therefore, the use of MSTN gene-edited channel catfish is of great importance for commercial farms.
Article
Fisheries
Karim Khalil, Ahmed Elaswad, Hisham Abdelrahman, Maximillian Michel, Wenbiao Chen, Shikai Liu, Ramjie Odin, Zhi Ye, David Drescher, Khoi Vo, William S. Bugg, Guyu Qin, Yujia Yang, Nathan J. C. Backenstose, Zhanjiang Liu, Roger D. Cone, Rex Dunham
Summary: Targeting the mc4r gene using the CRISPR-Cas9 system was performed in the channel catfish, a significant freshwater aquaculture species in the USA. Electroporation and microinjection were employed to deliver gRNA(s)-Cas9 mixtures into one-cell embryos. The overall mutation rates were 30.6% and 66.7-90.6% for electroporation and microinjection, respectively. The generated mutated fish showed improved body weight by 38% (electroporation) and 20% (microinjection), along with a reduced feed conversion ratio, suggesting the potential economic benefit of mc4r-edited fish in aquaculture production.
