Article
Biochemistry & Molecular Biology
Deyu Zhang, Qiwei Jiang, Xiangwei Ge, Yanzhu Shi, Tianxing Ye, Yue Mi, Tian Xie, Qihong Li, Qinong Ye
Summary: The study revealed a critical role of RHOV in LUAD metastasis, suggesting it as a potential biomarker for patient prognosis and a therapeutic target.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Virology
Alexandre Flageul, Chantal Allee, Celine Courtillon, Veronique Beven, Helene Quenault, Yannick Blanchard, Michel Amelot, David Courtois, Sjaak De Wit, Nicolas Eterradossi, Beatrice Grasland, Paul A. Brown
Summary: Despite the use of many live IBV vaccines, the Infectious Bronchitis virus (IBV) still causes significant economic losses in the chicken industry. Vaccine-induced partial protection may contribute to the emergence of new IBV strains. Through experiments on chickens, it was found that the IBV virus evolved rapidly in both vaccinated and non-vaccinated birds, but the genetic changes selected and their locations were different between the two groups.
Article
Multidisciplinary Sciences
Vasily Marchenko, Alexey Danilenko, Natalia Kolosova, Maria Bragina, Marina Molchanova, Yuliya Bulanovich, Vladimir Gorodov, Sergey Leonov, Andrey Gudymo, Galina Onkhonova, Svetlana Svyatchenko, Alexander Ryzhikov
Summary: This study found coronaviruses in birds in Russia with a 12% detection rate, including both Gammacoronavirus and Deltacoronavirus. Further research on the spread and diversity of coronaviruses among birds is warranted.
SCIENTIFIC REPORTS
(2022)
Article
Virology
Na Xing, Zhisheng Wang, Jichun Wang, Mariana Nascimento, Anan Jongkaewwattana, Jakob Trimpert, Nikolaus Osterrieder, Dusan Kunec
Summary: Reverse genetics systems based on bacterial artificial chromosomes (BACs) are essential tools in coronavirus research. In this study, a reverse genetics system was established for the avian coronavirus strain Beaudette, which is extensively studied. The infectious clone of the Beaudette strain was constructed as a BAC, allowing the recovery of an infectious virus identical to the parental virus. The system was used to generate reporter viruses expressing enhanced green fluorescent protein (EGFP), which can serve as a valuable tool for studying virus replication and developing antiviral drugs.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Eliana Icochea, Rosa Gonzalez, Gina Castro-Sanguinetti, Lenin Maturrano, Luis Alzamora, Luiz Sesti, Jorge Chacon, Juan More-Bayona
Summary: This study analyzed 200 samples collected from suspected infected animals in Peru between January and August in 2015, and found circulation of GI-16 and GI-1 lineages of infectious bronchitis virus (IBV). The GI-16 isolates showed unique nucleotide and amino acid changes compared to their closest relatives, suggesting potential relevance for vaccine evasion. These findings highlight the importance of genetic surveillance in improving vaccination strategies against infectious bronchitis.
Article
Multidisciplinary Sciences
Jiao Luo, Xiujun Tan, Ling Ye, Chenglin Wang
Summary: The study reveals that the JNK signaling pathway plays a positive role in the formation of dental papilla cell polarity, as indicated by the up-regulation of polarity-related genes and rescue effects of RhoA Q63L mutant. JNK activation is linked to polarization, migration, and differentiation of dental papilla cells, highlighting its importance in tooth development.
Article
Virology
Sarah Keep, Phoebe Stevenson-Leggett, Giulia Dowgier, Katalin Foldes, Isobel Webb, Albert Fones, Kieran Littolff, Holly Everest, Paul Britton, Erica Bickerton
Summary: Infectious bronchitis virus is a significant concern for the global poultry industry. Live-attenuated vaccines are generated by serial passage of a virulent isolate in embryonated eggs until attenuation is achieved. However, the exact mechanisms of attenuation are unknown and there is a risk of reversion to virulence.
JOURNAL OF VIROLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Jing Tan, Wei Gao, Wanchao Yang, Xianzhang Zeng, Linlin Wang, Xiaoguang Cui
Summary: JNK1 exacerbates ischemia-reperfusion injury in lung transplantation, while JNK2 alleviates it. JNK1 silencing attenuates lung graft dysfunction by inhibiting inflammation and apoptosis.
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2021)
Article
Microbiology
Shu-Yun Li, Yu-Xi Shen, Xue-Lian Xiang, Yong-Xin Li, Nian-Ling Li, An-Dong Wang, Min Cui, Xin-Feng Han, Yong Huang, Jing Xia
Summary: The study identified the L1089I substitution in the S2 subunit as a crucial determinant for viral nephrotropism in avian gamma-coronavirus infectious bronchitis viruses (IBV). This substitution significantly attenuated pathogenicity and disrupted viral replication and translation.
VETERINARY MICROBIOLOGY
(2023)
Article
Microbiology
Rui Gui, Huabin Zheng, Liping Ma, Renyi Liu, Xian Lin, Xianliang Ke, Chang Ye, Xiaoqin Jian, Quanjiao Chen
Summary: This study reveals for the first time the molecular mechanisms of cell death during influenza A virus infection and identifies a novel component, SPAG9. The findings have important implications for expanding our understanding of cell death mechanisms.
Article
Microbiology
Issam Hmila, Boutheina Marnissi, Masood Kamali-Moghaddam, Abdeljelil Ghram
Summary: Infectious bronchitis virus (IBV) is a major health problem in the poultry industry, causing high morbidity, high mortality, and large economic losses. The virus can mutate into new strains, leading to devastating disease and outbreaks.
MICROBIOLOGY SPECTRUM
(2023)
Article
Fisheries
Ping Zhong, Zhongneng Xu, Wei Chen, Kai Peng, Yuping Sun, Xiaopeng Wu, Huiming Sun, Xiaoying Chen, Peng Luo, Chaozheng Li, Huo Li, Wen Huang
Summary: 9C-Jun NH2-terminal kinase (JNK)-interacting protein 4 (JIP4) is identified and characterized in Litopenaeus vannamei, serving as an essential component in organizing and linking the MAPK pathway. Lv-JIP4 is widely expressed in various tissues, with high levels in muscle, and plays a potential role in activating and phosphorylating Lv-p38(MAPK) in response to low-temperature stress.
AQUACULTURE REPORTS
(2021)
Article
Multidisciplinary Sciences
Jake W. Willows, Gilian Gunsch, Emma Paradie, Magdalena Blaszkiewicz, Jeffrey R. Tonniges, Maria F. Pino, Steven R. Smith, Lauren M. Sparks, Kristy L. Townsend
Summary: Peripheral neuropathy, including axonal degeneration and demyelination, affects adipose tissues in obesity, diabetes, and aging. This study investigated the presence of demyelinating neuropathy in adipose tissue and found that adipose tissue contains myelinated and unmyelinated nerves populated by Schwann cells (SCs), which play a role in nerve regeneration. The study also showed that SCs in adipose tissue become dysregulated in diabetic peripheral neuropathy.
Article
Virology
Sarah Keep, Giulia Dowgier, Valeria Lulla, Paul Britton, Michael Oade, Graham Freimanis, Chandana Tennakoon, Christine Monceyron Jonassen, Torstein Tengs, Erica Bickerton
Summary: Coronaviruses infect many species, including humans and animals, with substantial effects on livestock, particularly with respect to poultry. The roles of the RNA structural elements in the coronavirus genome are poorly understood. This study investigated the requirement of the s2m structure in the replication of infectious bronchitis virus (IBV), a Gammacoronavirus that infects poultry causing respiratory disease. The results showed that deletion of the s2m sequence impacted viral replication and had a preference for nucleotide occupation, independent of the specific s2m sequence.
JOURNAL OF VIROLOGY
(2023)
Article
Physiology
Fan Yang, Elyce Ozols, Frank Y. Ma, Khai Gene Leong, Greg H. Tesch, Xiaoyun Jiang, David J. Nikolic-Paterson
Summary: The JNK signaling pathway plays a role in acute high dose AA-induced kidney injury, but is not essential in promoting chronic kidney disease caused by ongoing DNA damage from chronic low dose AA exposure.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Virology
Shumin Li, Siying Liu, Rui Ai Chen, Mei Huang, To Sing Fung, Ding Xiang Liu
Summary: This study reveals that coronavirus infection induces the expression of ZFP36, an anti-inflammatory protein, and promotes the degradation of transcripts coding for IL-6 and IL-8, as well as other proteins related to inflammation. The p38 MAP kinase and its upstream kinase MKK3, as well as downstream kinase MK2, play a regulatory role in the upregulation of ZFP36 during coronavirus infection. This MKK3-p38-MK2-ZFP36 axis could be a potential therapeutic target for severe coronavirus infections.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yu Shi, Xiaobei Yang, Xiaoxin Ye, Jiaying Feng, Tianfang Cheng, Xiaofan Zhou, Ding Xiang Liu, Linghui Xu, Junxia Wang
Summary: Citrus canker, caused by Xanthomonas citri subsp. citri, is a devastating disease that affects fruit quality and yield. This study found that a protein called HemK plays a crucial role in regulating the virulence traits of Xcc. Deletion of the hemK gene resulted in decreased motility, biofilm formation, extracellular enzymes, and polysaccharide production, as well as increased sensitivity to oxidative stress and high temperatures. HemK was also found to be essential for the virulence of Xcc on citrus plants and for the hypersensitive response on a non-host plant.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Li Xia Yuan, Bei Yang, To Sing Fung, Rui Ai Chen, Ding Xiang Liu
Summary: This study presents the transcriptomic profile of cells infected with gammacoronavirus infectious bronchitis virus (IBV) and reveals the differential expression of host genes and their related pathways. The study identifies 1162 differentially expressed genes, mainly enriched in MAPK and Wnt signaling pathways, in IBV-infected human non-small cell lung carcinoma cells (H1299 cells). The validation of induced transcripts in IBV-infected cells demonstrates the accuracy and reliability of the transcriptomic data. This study provides a reliable transcriptomic profile of host genes and pathways regulated by coronavirus infection.
Article
Virology
Chengwei Xiang, Zekun Yang, Ting Xiong, Ting Wang, Jie Yang, Mei Huang, Dingxiang Liu, Ruiai Chen
Summary: This study reveals that avian IRF7 and IRF1 play distinct roles in regulating the type I interferon response during DTMUV infection. They inhibit viral replication through the regulation of type I interferon expression and the induction of specific genes. Additionally, the study uncovers a strategy used by DTMUV to evade host immune surveillance via its non-structural protein 2B.
Article
Microbiology
Xiong Ting, Chengwei Xiang, Ding Xiang Liu, Ruiai Chen
Summary: This study developed a recombinant IBV using an efficient reverse genetics technology and found that it can provide broad protective immunity against two different genotypes of IBV.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Lixia Yuan, To Sing Fung, Jiawen He, Rui Ai Chen, Ding Xiang Liu
Summary: Coronaviruses exploit cellular processes and signalling pathways for their efficient reproduction. The AP-1 and EGR family of immediate-early response genes are activated by coronavirus infection. ERK1/2 is an upstream kinase, while JNK and p38 function as inhibitors of EGR1 activation. The upregulation of EGR1 has differential effects on viral replication, apoptosis, and antiviral response.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Biochemistry & Molecular Biology
Xinxin Sun, Li Quan, Ruiai Chen, Dingxiang Liu
Summary: This study demonstrates that coronavirus nonstructural protein 3 (nsp3) interacts with melanoma differentiation-associated gene 5 (MDA5), inhibiting the MDA5-mediated type I interferon (IFN) response. The study highlights the complex interaction between a multi-functional viral protein and the innate immune response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Chengwei Xiang, Zekun Yang, Ting Xiong, Ting Wang, Jie Yang, Mei Huang, Dingxiang Liu, RuiAi Chen
Summary: This study reports the construction of a DF-1 cell line with knockout IFNAR1 gene, which enhances the replication of DTMUV and activates genes and signaling pathways related to cell growth and apoptosis. Knockdown of two up-regulated genes, HELZ2 and IFI6, also increases the replication of DTMUV RNA.
Article
Immunology
Li Quan, Xinxin Sun, Linghui Xu, Rui Ai Chen, Ding Xiang Liu
Summary: Disruption of the cell cycle is a common strategy used by many viruses to create a favorable cellular microenvironment for their replication. In this study, we demonstrate that the gamma-coronavirus infectious bronchitis virus (IBV) nonstructural protein 12 (nsp12) interacts with the p50 regulatory subunit of DNA polymerase delta (pol delta), as well as with nsp13, to induce cell cycle arrest in the S phase. Interestingly, nsp12 from severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 also interacted with p50. These findings highlight the importance of these interactions in coronavirus replication and virus-host interaction, and suggest potential targets for antiviral intervention.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Virology
Si Ying Liu, Mei Huang, To Sing Fung, Rui Ai Chen, Ding Xiang Liu
Summary: Coronavirus infection induces a variety of cellular antiviral responses either dependent on or independent of type I interferons (IFNs). Our previous studies revealed the differential induction of three IFN-stimulated genes (ISGs), IRF1, ISG15 and ISG20, by gammacoronavirus infectious bronchitis virus (IBV) infection. This study characterized the induction kinetics and anti-IBV functions of these ISGs, and explored the mechanisms underlying their differential induction.
Article
Multidisciplinary Sciences
Xinyuan Cui, Kewei Fan, Xianghui Liang, Wenjie Gong, Wu Chen, Biao He, Xiaoyuan Chen, Hai Wang, Xiao Wang, Ping Zhang, Xingbang Lu, Rujian Chen, Kaixiong Lin, Jiameng Liu, Junqiong Zhai, Ding Xiang Liu, Fen Shan, Yuqi Li, Rui Ai Chen, Huifang Meng, Xiaobing Li, Shijiang Mi, Jianfeng Jiang, Niu Zhou, Zujin Chen, Jie-Jian Zou, Deyan Ge, Qisen Yang, Kai He, Tengteng Chen, Ya-Jiang Wu, Haoran Lu, David M. Irwin, Xuejuan Shen, Yuanjia Hu, Xiaoman Lu, Chan Ding, Yi Guan, Changchun Tu, Yongyi Shen
Summary: Wildlife is a reservoir for emerging viruses, and this study identified and characterized 27 families of mammalian viruses from wild and zoo animals in south China. Bats were found to have a high diversity of coronaviruses, picornaviruses, and astroviruses, as well as a potentially new genus of Bornaviridae. The study also discovered potential cross-species transmission of RNA and DNA viruses between wildlife and domestic animals, complicating disease prevention and control. Overall, the study provides important insights into zoonotic risk and the frequency of host-jumping events.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Ting Xu, Ting Xiong, Wenting Xie, Jing Wu, Xiao Liu, Guimin Li, Yadi Lv, Linyu Li, Zekun Yang, Han Wang, Dingxiang Liu, Ruiai Chen
Summary: Newcastle disease (ND) and infectious bursal disease (IBD) are two major infectious diseases that pose a significant threat to the poultry industry. This study constructed three recombinant adenoviruses, which have the potential to be safe and effective vaccine candidates for the prevention and control of ND and IBD.
Article
Chemistry, Analytical
Kunlun Jiang, Jung-Hoon Lee, To Sing Fung, Jingrui Wu, Congnuan Liu, Hua Mi, R. P. V. Jayanthe Rajapakse, Udeni B. R. Balasuriya, Yung-Kang Peng, Yun Young Go
Summary: The researchers have developed a simple and low-cost plasmonic photothermal (PPT)-reverse transcription-colorimetric polymerase chain reaction (RTcPCR) for molecular diagnosis of dengue virus (DENV) infection. The assay can be completed within 54 minutes and has a detection limit of 1.6 copies/μL of viral nucleic acid. The clinical performance evaluation showed a diagnostic agreement of 97.5% compared to the reference RT-qPCR.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Mei Huang, Hanghui Zheng, Weixiong Tan, Chengwei Xiang, Niran Fang, Wenting Xie, Lianghai Wen, Dingxiang Liu, Ruiai Chen
Summary: Immunization of pigs with SFV replicon-based vaccine candidates can promote specific T cell and humoral immunity against ASFV. Individual heterogeneity in response to the vaccine requires personalized analysis. Signaling pathways mediated by Toll-like receptors, C-type lectin receptors, IL17 receptors, NOD-like receptors, and nucleic acid sensors are correlated with antibody production and IFN-γ secretion in PBMCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)