Article
Cell Biology
Yanjie Tan, Jing Zhang, Yi Jin
Summary: Skeletal muscle regeneration is a vital physiological process that responds to injury or disease. Nonsense-mediated mRNA decay (NMD) has been found to inhibit myoblast differentiation by targeting the phosphoinositide-3-kinase regulatory subunit 5 gene, leading to the suppression of MyoD's transcriptional activity. Inhibiting NMD can accelerate muscle regeneration, making it a potential therapeutic target for muscle-related injuries and diseases.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Geriatrics & Gerontology
Nikki M. McCormack, Eric Villalon, Coralie Viollet, Anthony R. Soltis, Clifton L. Dalgard, Christian L. Lorson, Barrington G. Burnett
Summary: This study found reduced expression of muscle fusion proteins as a key factor in the fusion deficits of SMN-deficient myoblasts. Ectopic expression of the fusion protein myomixer improved myofibre number and motor function in SMA model mice. Targeting muscle fusion proteins could enhance clinical outcomes for SMA patients in combination with SMN increasing therapy.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2021)
Article
Biochemistry & Molecular Biology
Dongdong Wang, Dandan Zhao, Yuan Li, Tingjun Dai, Fuchen Liu, Chuanzhu Yan
Summary: In this study, we identified TGM2 as a regulator of muscle differentiation and regeneration. TGM2 promotes myoblast differentiation through enhancing GPR56 mediated activation of the mTOR signaling. Knockdown of TGM2 hinders muscle regeneration in a skeletal muscle injury model, and both TGM2 and activation of the mTOR signaling are up-regulated in muscles of patients with immune-mediated necrotizing myopathy.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Agriculture, Multidisciplinary
Jingjie Zhang, Mengjun Zheng, Linyue Zhou, Xinping Li, Yonghui Yu, Jing Wang, Baoguo Sun
Summary: This study found that oat beta-glucan can reverse tumor necrosis factor-alpha induced abnormal myoblast differentiation and reduce muscle atrophy. The similar effects were observed when NLRP3 or FoxO1 inhibitors were used. Exposure to beta-glucan or AS1842856 inhibited TNF-alpha induced activation of TLR4/NF-kappa B pathway and decreased the expression of NLRP3. These findings indicate that oat beta-glucan plays essential roles in promoting myoblast differentiation and alleviating muscle atrophy by inactivating FoxO1 and NLRP3 inflammasome signal pathway.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Agriculture, Multidisciplinary
Dingding Zhang, Jingli Tao, Xuan Zhang, Xiangfei Ma, Chengyu Li, Hongmin Li, Weijian Li, Jie Chen, Honglin Liu
Summary: This study reveals the role of neoruscogenin in muscle growth by inhibiting MSTN maturation and promoting protein synthesis metabolism. It also shows its potential in repairing muscle injuries. These findings have important implications for the treatment of muscle disorders and the livestock industry.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Xin Guan, Zihe Pan, Zhuoya Xu, Sidun Zhang, Haohao Tang, Guocheng Du, Jingwen Zhou
Summary: This study investigated the effects of four representative flavonoids on porcine myoblasts and found that these flavonoids had relatively low cytotoxicity but showed a weak ability to promote cell proliferation. Luteolin, one of the flavonoids, was found to have a positive effect on migration and differentiation of porcine myoblasts. The activation of the PI3K/Akt/mTOR signaling pathway was identified as the mechanism behind luteolin's effects. This study provided biological evidence for the application of luteolin in cultured meat production.
Article
Cell Biology
Xingzuan Lin, Peng Wang, Wei Wang, Hao Zhou, Siyuan Zhu, Shujing Feng, Yuzhou Chen, Han Zhou, Qichao Wang, Hanlong Xin, Xiexiang Shao, Jianhua Wang
Summary: Muscular fatty infiltration is a common pathology after rotator cuff tears, mainly caused by fibro-adipogenic progenitors (FAPs). This study found that fatty infiltration is more severe in the supraspinatus muscle compared to the gastrocnemius muscle after tendon injury. In vitro experiments showed that RCT-FAPs have a higher ability for adipogenic differentiation. Activation of Akt/GSK-3 beta/beta-catenin signaling decreased adipogenesis in RCT-FAPs, while Wnt signaling activation limited adipogenesis and improved shoulder function in a RCT model.
CELL DEATH DISCOVERY
(2023)
Article
Orthopedics
Mengjie Chen, Haibing Chen, Yonggui Gu, Peng Sun, Jianxiong Sun, Haojun Yu, Hongliang Zheng, Donghui Chen
Summary: The study reveals that purinergic receptor P2Y2 plays a crucial role in skeletal muscle atrophy and fibrosis, regulating the activity of muscle cells and fibroblasts after muscle injury to impact the development of the pathology.
BMC MUSCULOSKELETAL DISORDERS
(2021)
Article
Biochemistry & Molecular Biology
Hiroki Hamaguchi, Kitora Dohi, Takaomi Sakai, Masato Taoka, Toshiaki Isobe, Tsubasa S. Matsui, Shinji Deguchi, Yasuro Furuichi, Nobuharu L. Fujii, Yasuko Manabe
Summary: Myokines, such as platelet-derived growth factor sub-unit B (PDGF-B), play a crucial role in regulating muscle mass and function by acting locally on muscle cells or satellite cells. This study found that PDGF-B is continuously secreted by muscle cells and its secretion increases during myoblast to myotube differentiation. Treatment with PDGF-B enhances myoblast proliferation and promotes myotube maturation, as evidenced by increased myotube diameter, myosin heavy chain expression, and contractile force. These findings suggest that PDGF-B acts as a paracrine or autocrine myokine to contribute to skeletal muscle regeneration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Integrative & Complementary Medicine
Sang Hee Park, Dong Seon Kim, Jieun Oh, Jeong-Ho Geum, Jung-Eun Kim, Su-Young Choi, Ji Hye Kim, Jae Youl Cho
Summary: Chamomile extract MC shows significant effects on muscle atrophy by recovering muscle volume, density, and weight loss, increasing moving distance and grip strength. Its mechanism of action may involve inhibition of MuRF1 mRNA level, and enhancement of TFAM, MyoD, and Myogenin-1 expression.
AMERICAN JOURNAL OF CHINESE MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Huan Liu, Dengke Pan, Pu Li, Dandan Wang, Bo Xia, Ruixin Zhang, Junfeng Lu, Xiangyang Xing, Jiaxiang Du, Xiao Zhang, Long Jin, Lin Jiang, Linong Yao, Mingzhou Li, Jiangwei Wu
Summary: A novel sepsis pig model is created by modifying the surgical strategy of cecal ligation and puncture (CLP), which for the first time recapitulates the whole course of sepsis in humans. Increased levels of ZBED6 in skeletal muscle are shown in this model and sepsis patients, and protection against sepsis-induced muscle wasting is demonstrated in ZBED6-deficient pigs. The direct target of ZBED6 is revealed to be DOCK3, and septic ZBED6-deficient pigs show increased DOCK3 expression in skeletal muscle and myocytes, activating the RAC1/PI3K/AKT pathway and protecting against sepsis-induced muscle wasting.
Article
Chemistry, Medicinal
Chi-Woo Lee, Yeok Boo Chang, Chun Woong Park, Sung Hee Han, Hyung Joo Suh, Yejin Ahn
Summary: Adding Spirulina hydrolysates (SPH) to the cell culture medium can promote the differentiation of C2C12 myoblasts and inhibit Dexamethasone (DEX)-induced myotube atrophy. This is achieved by regulating the expression of muscle atrophy-related factors and regulating the FoxO3a transcription factor.
Article
Biology
Ra-Yeong Choi, Bong Sun Kim, Eu-Jin Ban, Minchul Seo, Joon Ha Lee, In-Woo Kim
Summary: This study found that mealworm ethanol extract (TME) can promote myotube formation and increase the expression of muscle-related proteins, enhancing muscle hypertrophy through regulating the mTOR signaling pathway. In the DEX-induced muscle atrophy C2C12 model, TME can reduce the expression of protein degradation-related genes and alleviate Dex-induced muscle atrophy by activating Akt. Therefore, TME has the potential to be a therapeutic agent for treating muscle weakness and atrophy.
Article
Biochemistry & Molecular Biology
Chong Dai, Adriana Reyes-Ordonez, Jae-Sung You, Jie Chen
Summary: Aminoacyl-tRNA synthetases play essential roles in protein synthesis, with some also exhibiting non-translational functions. Threonyl-tRNA synthetase (ThrRS) has been found to negatively regulate myoblast differentiation through a non-catalytic mechanism involving new domains UNE-T and TGS. ThrRS interacts with Axin1, inhibiting JNK signaling and affecting skeletal myogenesis through the MEKK4-MKK4-JNK pathway.
Article
Plant Sciences
Ryuni Kim, Jee Won Kim, Hyerim Choi, Ji-Eun Oh, Tae Hyun Kim, Ga-Yeon Go, Sang-Jin Lee, Gyu-Un Bae
Summary: This study reveals the effects of Ginsenoside Rg5 on muscle differentiation, growth, and prevention of muscle atrophy. Rg5 promotes myogenesis and hypertrophy by activating p38MAPK and Akt/mTOR signaling pathways. This research suggests that Rg5 has potential therapeutic applications in muscle weakness and atrophy.
JOURNAL OF GINSENG RESEARCH
(2023)
Article
Nutrition & Dietetics
Kyungtae Kim, Ki Hong Nam, Sang Ah Yi, JongWoo Park, Jeung-Whan Han, Jaecheol Lee
Article
Plant Sciences
Seulah Lee, Dahae Lee, Rhim Ryoo, Jin-Chul Kim, Hyun Bong Park, Ki Sung Kang, Ki Hyun Kim
JOURNAL OF NATURAL PRODUCTS
(2020)
Article
Plant Sciences
Mi Young Byun, Dockyu Kim, Ui Joung Youn, Seulah Lee, Hyoungseok Lee
Summary: This study evaluated the effects of humic acids (HA) on the growth and photosynthesis of moss species, finding that increasing HA concentrations correlated with increased growth and photosynthesis in polar mosses. However, the efficiency for temperate mosses increased at lower concentrations but began to reduce at the highest HA concentration.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Chemistry, Organic
Seulah Lee, Chung Sub Kim, Jae Sik Yu, Heesun Kang, Min Jeong Yoo, Ui Joung Youn, Rhim Ryoo, Han Yong Bae, Ki Hyun Kim
Summary: A new ergosterol derivative, ergopyrone, along with five related metabolites, was isolated and characterized from a mushroom. The unprecedented hexacyclic 6/5/6/6/6/5 skeleton of ergopyrone suggests a unique biosynthetic pathway. Compound 6 was found to significantly regulate lipid metabolism through mRNA expression modulation.
Article
Biochemistry & Molecular Biology
Sang Keun Ha, Min Cheol Kang, Seulah Lee, Om Darlami, Dongyun Shin, Inwook Choi, Ki Hyun Kim, Sun Yeou Kim
Summary: A stilbene glycoside with a unique sugar unit was identified through biotransformation, showing regenerative and anti-inflammatory effects similar to resveratrol. The compound was found to be safe and stable, suggesting the biotransformation method as an efficient platform for producing natural glycosides with enhanced safety.
Article
Chemistry, Medicinal
Yong Joo Park, Mi Seon Jeon, Seulah Lee, Jung Kyu Kim, Tae Su Jang, Kyu Hyuck Chung, Ki Hyun Kim
Summary: The study found that brevilin A has a reversing effect on the expression levels of HSC activation marker α-SMA and ECM proteins induced by TGF-β1. Moreover, brevilin A inhibits STAT3 phosphorylation and decreases the expression levels of fibronectin and connective tissue growth factor induced by TGF-β1, indicating its potential in preventing and treating hepatic fibrosis.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Microbiology
Dockyu Kim, Ha Ju Park, Mincheol Kim, Seulah Lee, Soon Gyu Hong, Eungbin Kim, Hyoungseok Lee
Summary: In the maritime Antarctic tundra, long-term warming or prolonged thawing periods can impact the relative abundance of cold-adapted bacterial communities, thereby promoting the rate of microbial decomposition of heteropolymer humic substances.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jung-Hoon Pyun, Byeong-Yun Ahn, Tuan Anh Vuong, Su Woo Kim, Yunju Jo, Jaehyung Jeon, Seung Ho Baek, Jaewon Kim, Sungsu Park, Gyu-Un Bae, Jun-Hyuk Choi, Jae-Ryong Kim, Dongryeol Ryu, Sang-Jin Lee, Jong-Sun Kang
Summary: Prmt1 plays a crucial role in maintaining vascular smooth muscle cell function, and its ablation may lead to aortic diseases. Ablation of Prmt1 affects the expression of contractile genes, causing VSMCs to switch phenotypes and resulting in aortic dilation and dissection.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Jae Hyeon Kim, Sunho Kim, Surim Han, Eun-Kyung Ahn, Young-Rak Cho, Wonsik Jeong, Sung Joon Kim, Gyu-Un Bae, Joa Sub Oh, Dong-Wan Seo
Summary: In this study, the regulatory effects and mechanism of broussonin A and B on endothelial cell responses and angiogenesis were investigated. The results showed that broussonin A and B suppressed VEGF-A-stimulated endothelial cell proliferation and angiogenic responses through the regulation of cell cycle-related proteins and downstream signaling pathways. Furthermore, broussonin A and B inhibited the growth and invasion of lung and ovarian cancer cells. These findings suggest the potential pharmacological use of broussonin A and B in the regulation of angiogenesis and cancer cell growth.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Seulah Lee, Jae Sik Yu, Seoung Rak Lee, Ki Hyun Kim
Summary: This review summarizes the structures and biological activities of secondary metabolites identified from poisonous mushrooms, providing an overview of the current information on these metabolites with a focus on their chemistry, bioactivity, and biosynthesis.
NATURAL PRODUCT REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Seulah Lee, Se Yun Jeong, Dieu Linh Nguyen, Jae Eun So, Ki Hyun Kim, Ji Hee Kim, Se Jong Han, Sung-Suk Suh, Jun Hyuck Lee, Ui Joung Youn
Summary: This study isolated two new compounds from Antarctic lichen and characterized their structures. The new cyclic depsipeptide showed moderate antimicrobial activity against E. coli, while a dibenzofuran derivative exhibited cell growth inhibition against HCT116 cells and potent anti-inflammatory activity against RAW264.7 macrophages.
Article
Chemistry, Medicinal
Seulah Lee, Tae Wan Kim, Yong Hoon Lee, Dong-Min Kang, Rhim Ryoo, Yoon-Joo Ko, Mi-Jeong Ahn, Ki Hyun Kim
Summary: This study identified and isolated fatty acid derivatives from a poisonous mushroom, which showed promising antibacterial activity against Helicobacter pylori. One of the compounds exhibited significant inhibitory effects, suggesting its potential as a candidate for novel antibiotic development against H. pylori.
Article
Chemistry, Medicinal
Seulah Lee, Akida Alishir, Tae Wan Kim, Dong-Min Kang, Rhim Ryoo, Changhyun Pang, Mi-Jeong Ahn, Ki Hyun Kim
Summary: This study conducted the first chemical investigation of Amanita hemibapha subsp. javanica, an edible Korean wild mushroom. Compound 4 isolated from the fruiting bodies showed promising antibacterial activity against Helicobacter pylori, suggesting its potential as a natural antibiotic for the development of novel antibiotics.
Article
Biochemistry & Molecular Biology
Kim-Hoa Phi, Min-Ji Shin, Seulah Lee, Jae Eun So, Ji Hee Kim, Sung-Suk Suh, Man Hyung Koo, Seung Chul Shin, Jin-Hyoung Kim, Jun Hyuck Lee, Ui Joung Youn
Summary: Three p-terphenyls were isolated from the Antarctic lichen Stereocaulon alpinum, including two new compounds. Compound 3 showed cytotoxicity against HCT116 cells and inhibited NO production in LPS-induced RAW264.7 macrophages.
Article
Biochemistry & Molecular Biology
Seulah Lee, Man Hyung Koo, Dong-Won Han, Il-Chan Kim, Jun Hyuck Lee, Jeong-Hoon Kim, Razia Sultana, Sun Yeou Kim, Ui Joung Youn, Jin-Hyoung Kim
Summary: This study analyzed the fatty acid composition of different organs of two Antarctic fish species (marbled rockcod and mackerel icefish) using GC-MS. The results showed variations in the fatty acid composition between the two fish species and differences in organ specificity. Additionally, extracts from the skin and liver of marbled rockcod exhibited significant inhibitory effects on matrix metalloproteinase-1.
