Effect of orally administered exopolysaccharides produced by Lactococcus lactis, subsp cremoris FC on a mouse model of dermatitis induced by repeated exposure to 2,4,6-trinitro-l-chlorobenzene

Lactococcus lactis subsp. cremoris (L cremoris) FC is a lactic acid bacteria that produces exopolysaccharides (EPS). We studied the effects of EPS on the development of dermatitis in BALB/c mice induced by repeated exposure to 2,4,6-trinitro-1 -chlorobenzene (TNCB) and on intestinal immunological activity in C3H/HeJ mice through Peyer's patch cells. The EPS was shown to be composed of phosphopolysaccharides containing rhamnose, galactose, and glucose in a molar ratio of 1:1:3. Their weight average molecular weight (MW) was 712,000. Oral administration of EPS suppressed skin thickening and infiltration of mast cells in skin lesions. EPS suppressed IL-4, IFN-gamma, IL-6, and TNF-alpha overexpression caused by exposure to TNCB. Peyer's patch cell medium cultured with EPS stimulated bone marrow cell proliferation. Together, these results suggest that EPS produced by L cremoris FC may be an effective dietary component to prevent chronic skin inflammatory diseases. (C) 2017 Elsevier Ltd. All rights reserved.