Article
Biochemistry & Molecular Biology
Zhilei Li, Lang He, Xia Wang, Qingwen Huo, Guosong Zheng, Dekun Kong, Yinhua Lu, Haiyang Xia, Guoqing Niu
Summary: Sideromycins are a unique subset of siderophores that combine a siderophore and an antimicrobial agent. This study deciphered the biosynthetic pathway of the ferrichrome-type siderophore in Streptomyces sp. ATCC 700974 and identified the involved genes. The findings provide important insights into the synthesis of albomycin and the role of multiple siderophores in Streptomyces sp. ATCC 700974.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Masayuki Igarashi, Ryuichi Sawa, Maya Umekita, Masaki Hatano, Rie Arisaka, Chigusa Hayashi, Yoshimasa Ishizaki, Masato Suzuki, Chiaki Kato
Summary: A strain of Nonomuraea sp. MM565M-173N2 isolated from deep-sea sediment off the Sanriku coast yielded four new antibiotics, with sealutomicin A showing strong antibacterial activity against carbapenem-resistant Enterobacteriaceae (CRE) in particular.
JOURNAL OF ANTIBIOTICS
(2021)
Article
Chemistry, Organic
Zhuangjie Fang, Qingbo Zhang, Weiliang Xiong, Lili Sun, Bin Tan, Mengyi Zhu, Liang Ma, Liping Zhang, Yiguang Zhu, Changsheng Zhang
Summary: In this study, four new tetronate antibiotics called kongjuemycins (KJMs) were discovered from a coral-associated actinomycete. The biosynthetic gene cluster responsible for KJMs production was identified and characterized through various experiments. It was found that KJM biosynthesis utilizes diverse precursors from primary metabolism.
Article
Microbiology
Songbiao Shi, Linqing Cui, Kun Zhang, Qi Zeng, Qinglian Li, Liang Ma, Lijuan Long, Xinpeng Tian
Summary: Marine actinomycetes are a significant source of antibiotics, and in this study, two newly discovered marine actinobacterial strains, named Streptomyces marincola, show potential in producing unique bioactive natural products through genome mining and in silico analysis, highlighting their importance in both marine ecology and industrial applications.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Qiyao Shen, Guangzhi Dai, Aiying Li, Yang Liu, Guannan Zhong, Xiaoju Li, Xiangmei Ren, Haiyan Sui, Jun Fu, Nianzhi Jiao, Youming Zhang, Xiaoying Bian, Haibo Zhou
Summary: Genome mining of the marine actinomycete Saccharothrix sp. D09 led to the discovery of a potentially novel polycyclic aromatic polyketide. Through specific strategies and metabolite analysis, 20 angucycline derivatives were successfully identified, some of which showed antibacterial and anti-inflammatory activity.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Microbiology
Zhen-Yu Zan, Xian-Feng Ge, Rui-Rui Huang, Wen-Zheng Liu
Summary: A novel actinomycete strain was isolated from a mangrove soil sample and its taxonomic position was determined using a polyphasic approach. It was identified as a new species of the genus Pseudonocardia based on phylogenetic analysis and genotypic properties.
CURRENT MICROBIOLOGY
(2022)
Article
Plant Sciences
Longji Wang, Ting Lei, Guomin Han, Junyang Yue, Xueru Zhang, Qi Yang, Haixiang Ruan, Chunyang Gu, Qiang Zhang, Tao Qian, Niuniu Zhang, Wei Qian, Qi Wang, Xiaojing Pang, Yue Shu, Liping Gao, Yunsheng Wang
Summary: Rubus chingii, a traditional Chinese medicinal plant, has a chromosome-scale genome that provides insights into the biosynthesis of hydrolyzable tannins. Key genes related to hydrolyzable tannin synthesis were identified in the genome of R. chingii, which may play essential roles in this process.
Article
Chemistry, Organic
Shuai Li, Lu-Ping Chi, Zhong Li, Mingyu Liu, Ruxin Liu, Moli Sang, Xianliang Zheng, Lei Du, Wei Zhang, Shengying Li
Summary: This study successfully activated a transcriptionally silent polyketide biosynthetic gene cluster in Streptomyces using CRISPR/Cas9 genome editing technology, leading to the discovery of two new polyketide metabolites. The findings revealed an unusual characteristic of the polyketide synthases during their biosynthesis.
Article
Microbiology
Slimane Khayi, Andrew D. Armitage, Fatima Gaboun, Issam Meftah-Kadmiri, Rachid Lahlali, Mohamed Fokar, Rachid Mentag
Summary: This study presents a high-quality genome assembly of the virulent isolate Foa 44, providing valuable insights into the genetic basis of Bayoud disease. It also reveals the distinct clade of FOA isolates within the Fusarium oxysporum species complex and sheds light on the genealogies of certain Secreted In Xylem (SIX) genes in FOA.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biology
Patrick Jung, Paul M. D'Agostino, Katharina Brust, Burkhard Buedel, Michael Lakatos
Summary: This study identified the taxonomic position of strain PCC 6712 using morphology, genome analysis, and phylogeny, as well as detected shared genes with its closest relative and potential bioactive gene clusters. The presence of a full set of nitrogen fixation genes in PCC 6712, despite its inability to grow on nitrogen-free medium, was also highlighted. Additionally, a comparison of genes responsible for multicellularity revealed important aspects of cyanobacterial taxonomy.
Article
Microbiology
Marina Sanchez-Hidalgo, Maria Jesus Garcia, Ignacio Gonzalez, Daniel Oves-Costales, Olga Genilloud
Summary: In this study, a strain of Kribbella sp. CA-293567 was found to produce sandramycin and kribbellichelins A-B, which are new secondary metabolites. The complete genome sequencing and in silico identification of biosynthetic gene clusters were performed, focusing on the pathways encoding these compounds. A comparative analysis of the biosynthetic potential of 38 publicly available genomes from Kribbella strains was also presented.
Article
Microbiology
Chuckcris P. P. Tenebro, Dana Joanne V. L. Trono, Lex Aliko P. Balida, Leah Katrine A. Bayog, Julyanna R. R. Bruna, Edna M. M. Sabido, Dion Paul C. Caspe, Emmanuel Lorenzo C. de los Santos, Jonel P. P. Saludes, Doralyn S. S. Dalisay
Summary: A novel actinomycete strain, Streptomyces tubbatahanensis sp. nov., was isolated from underexplored marine sediments in Tubbataha Reefs Natural Park, Philippines. The strain exhibited antibacterial and anticancer activities, and its specialized metabolites were characterized using mass spectrometry and nuclear magnetic resonance analyses. The genome of S. tubbatahanensis DSD3025(T) encoded 29 putative biosynthetic gene clusters, including a unique region not found in its close relatives.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Organic
Mahmud T. Morshed, Ernest Lacey, Daniel Vuong, Alastair E. Lacey, Soo Sum Lean, Stephen A. Moggach, Peter Karuso, Yit-Heng Chooi, Thomas J. Booth, Andrew M. Piggott
Summary: Using LCMS-guided screening, a bacterium called Streptomyces sp. MST-144321 was identified as a prolific producer of chlorinated metabolites. The study isolated and characterized compounds from MST-144321, revised the stereostructures of some compounds, and suggested a potential role of superclusters in the evolution of biosynthetic gene clusters.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Ji-Hee Park, Heung-Soon Park, Hee-Ju Nah, Seung-Hoon Kang, Si-Sun Choi, Eung-Soo Kim
Summary: Through the construction and screening of a bacterial artificial chromosome (BAC) library, a large-sized NPP B1 biosynthetic gene cluster (BGC) was successfully isolated from P. autotrophica chromosome. Additional introduction of the isolated BAC clone led to a significant increase in the production level of NPP B1 in P. autotrophica, while co-expression of a portion including the missing 8 kb further enhanced the production. The study suggests that Streptomyces BAC cloning of large-sized natural product BGCs is a valuable approach for improving production yields and screening biological activities of natural products in actinomycetes.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Shunji Takahashi
Summary: This review provides an overview of biosynthetic studies investigating the production of reveromycin in Streptomyces sp. SN-593 and explores the importance of biosynthetic mechanism and gene expression. Furthermore, by optimizing the expression of pathway-specific regulators, Streptomyces sp. SN-593 has been developed as a platform for mass production of terpenoid compounds.
JOURNAL OF ANTIBIOTICS
(2022)
Editorial Material
Plant Sciences
Mohammad R. Seyedsayamdost, Pierre Stallforth
JOURNAL OF NATURAL PRODUCTS
(2020)
Article
Chemistry, Multidisciplinary
Chen Zhang, Mohammad R. Seyedsayamdost
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Chemistry, Physical
Clarissa C. Forneris, Andy K. L. Nguy, Mohammad R. Seyedsayamdost
Article
Biochemistry & Molecular Biology
Aya Yoshimura, Brett C. Covington, Etienne Gallant, Chen Zhang, Anran Li, Mohammad R. Seyedsayamdost
ACS CHEMICAL BIOLOGY
(2020)
Review
Biotechnology & Applied Microbiology
Jennifer R. Klaus, Pauline M. L. Coulon, Pratik Koirala, Mohammad R. Seyedsayamdost, Eric Deziel, Josephine R. Chandler
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Brett C. Covington, Fei Xu, Mohammad R. Seyedsayamdost
Summary: Microbial natural products are important for therapeutic leads and research innovation. Silent biosynthetic gene clusters in bacteria contain hidden reservoirs of potential natural products. Various methods have been developed to interrogate these silent clusters, with different strategies and approaches for each category.
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 90, 2021
(2021)
Article
Multidisciplinary Sciences
Aidin R. Balo, Alessio Caruso, Lizhi Tao, Dean J. Tantillo, Mohammad R. Seyedsayamdost, R. David Britt
Summary: Using electron paramagnetic resonance (EPR) spectroscopy, this study dissected the mechanism of SuiB and revealed the essential role of the auxiliary [4Fe-4S] cluster in catalysis, confirming the proposed radical electrophilic aromatic substitution (rEAS) mechanism for the formation of the Lys-Trp cross-link. The findings provide direct evidence for the involvement of the SPASM domain auxiliary cluster and support rEAS as the mechanistic paradigm for rSAM enzyme-catalyzed carbon-carbon bond-forming reactions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Britta E. Rued, Brett C. Covington, Leah B. Bushin, Gabriella Szewczyk, Irina Laczkovich, Mohammad R. Seyedsayamdost, Michael J. Federle
Summary: This study identified a quorum-sensing system in Streptococcus mutans that regulates the production of a novel posttranslationally modified peptide capable of inhibiting the growth of other streptococcal species. It was found that a similar peptide produced by S. ferus also exhibited inhibitory properties, suggesting a role in interspecies competition in the oral microbiota.
Article
Chemistry, Organic
Alessio Caruso, Mohammad R. Seyedsayamdost
Summary: QmpB enzyme catalyzes reaction by installing two 9-membered ring sactionine bridges between Cys residue and upstream Asn residue, making it the second characterized type II sactionine synthase to date.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Anran Li, Bethany K. Okada, Paul C. Rosen, Mohammad R. Seyedsayamdost
Summary: Natural products are important sources of therapeutic agents and chemical tools. This study identified beta-lactams, fluoroquinolones, antifungals, and unexpected drugs as potent inducers of biosynthetic genes in Burkholderia thailandensis, shedding light on the regulatory mechanisms of these genes. The work uncovers a diverse array of elicitors and previously unknown regulatory inputs for secondary metabolism in B. thailandensis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Brett C. Covington, Mohammad R. Seyedsayamdost
Summary: Advancements in DNA sequencing, bioinformatics, and mass spectrometry-based metabolite detection have led to a new era of natural product discovery, necessitating the use of data analysis software to explore and map complex microbial secondary metabolomes. MetEx is a highly interactive web application that allows for visualization and analysis of multidimensional datasets, enabling prioritization, data organization, and dereplication for the discovery of new natural products.
ACS CHEMICAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Jong-Duk Park, Yuchen Li, Kyuho Moon, Esther J. Han, Seoung Rak Lee, Mohammad R. Seyedsayamdost
Summary: The study reports eight novel and architecturally unusual secondary metabolites synthesized by the bacterial symbiont Phaeobacter inhibens, which may originate from their algal hosts in a natural setting. Some of these metabolites exhibit potent algaecidal activity, providing important implications for the parasitic phase of algal-bacterial symbiotic interactions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Organic
Karoline G. Primdahl, Asmund Kaupang, Jong-Duk Park, Mohammad R. Seyedsayamdost, Jens M. J. Nolsoe, Marius Aursnes
SYNTHESIS-STUTTGART
(2022)
Correction
Microbiology
Britta E. Rued, Brett C. Covington, Leah B. Bushin, Gabriella Szewczyk, Irina Laczkovich, Mohammad R. Seyedsayamdost, Michael J. Federle
Article
Biochemistry & Molecular Biology
Samantha M. Horwitz, Tamra C. Blue, Joseph A. Ambarian, Shotaro Hoshino, Mohammad R. Seyedsayamdost, Katherine M. Davis
Summary: This study reports the structural interactions between HAQs and DHODH, revealing the inhibition mechanism and providing a foundation for further structure-activity relationship studies.
RSC CHEMICAL BIOLOGY
(2022)