Article
Chemistry, Analytical
Saeyeong Jeon, Youjin Lee, Daeho Ryu, Yoon Kyung Cho, Yena Lee, Sang Beom Jun, Chang-Hyeon Ji
Summary: Optogenetics has become crucial in neuroscience research due to its cell-type-specific neuromodulation feature. Technological advances in light delivery devices have allowed for the combination of optogenetics and electrophysiology, as demonstrated by a novel optrode array design for optical modulation and electrophysiological recording. In vivo experiments with transgenic mice expressing channelrhodopsin have confirmed the effectiveness of optical activation and neural recording.
Article
Engineering, Biomedical
Caleb Sponheim, Vasileios Papadourakis, Jennifer L. Collinger, John Downey, Jeffrey Weiss, Lida Pentousi, Kaisa Elliott, Nicholas G. Hatsopoulos
Summary: Microelectrode arrays, specifically Utah electrode arrays (UEAs), are widely used in chronic electrophysiological experiments for recording neural activity. This study evaluated the reliability and longevity of UEAs over almost nine years in 17 rhesus macaques and 2 human subjects, finding an average lifespan of 622 days with some arrays lasting over 1000 days and one up to 9 years. Results suggest chronic unit recordings can last at least two years, with some arrays potentially lasting up to a decade.
JOURNAL OF NEURAL ENGINEERING
(2021)
Article
Chemistry, Analytical
Changhua You, Lei Yao, Pan Yao, Li Li, Ping Ding, Shuli Liang, Chunxiu Liu, Ning Xue
Summary: This paper proposes a compact bioelectronics sensing platform that includes a multi-channel electrode, intracranial electroencephalogram (iEEG) recorder, adjustable galvanometer, and shunt-current conduction circuit pathway. The developed implantable electrode made of polyurethane-insulated stainless-steel materials is capable of recording iEEG signals and shunt-current conduction. The experimental results verify the potential of this platform and its important application value in the treatment of epilepsy.
Article
Biochemical Research Methods
Weiyang Yang, Yan Gong, Cheng-You Yao, Maheshwar Shrestha, Yaoyao Jia, Zhen Qiu, Qi Hua Fan, Arthur Weber, Wen Li
Summary: Integrative neural interfaces combining neurophysiology, optogenetics, and neural imaging offer opportunities for studying neural circuits in the brain. The development of ultra-flexible, highly conductive and fully transparent microscale electrocorticogram (mu ECoG) electrode arrays with PEDOT:PSS-ITO-Ag-ITO assembly shows enhanced mechanical flexibility, robustness, and resilience to photon-induced artifacts. In vivo experiments demonstrate successful recording of light-evoked ECoG oscillations from the primary visual cortex (V1) of an anesthetized rat.
Article
Chemistry, Multidisciplinary
Dongxin Xu, Jiaru Fang, Mingyue Zhang, Qijian Xia, Hongbo Li, Ning Hu
Summary: New strategies for intracellular electrophysiology using nanotemplate electrodes provide sensitive and prolonged recording of action potentials, leading to significant advances in the field of cardiology and neuroscience.
Article
Engineering, Electrical & Electronic
Penghai Li, Juanjuan Huang, Mingji Li, Hongji Li
Summary: In this study, PDMS filled with copper sulphate crystals, TiO2 nanoparticles, and carbon nanotubes was used as a flexible matrix to fabricate semi-dry multi-claws and 19-channel electrodes for EEG recording. These flexible electrodes showed good adhesion and reduced contact resistance, resulting in accurate and stable EEG signal recording.
SENSORS AND ACTUATORS A-PHYSICAL
(2022)
Article
Engineering, Electrical & Electronic
Alessandra Galli, Nicolo Lago, Sarah Tonello, Mario Bortolozzi, Marco Buonomo, Morten Gram Pedersen, Andrea Cester, Giada Giorgi
Summary: This paper proposes a processing technique to accurately and reliably extract single-cell action potentials from signals acquired by extracellular recording devices. The proposed method utilizes denoising and threshold-based classification techniques to improve the performance of peak detection. The results of tests on simulated and experimental data demonstrate the effectiveness of the proposed approach.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2022)
Article
Engineering, Biomedical
Elissa J. Welle, Joshua E. Woods, Ahmad A. Jiman, Julianna M. Richie, Elizabeth C. Bottorff, Zhonghua Ouyang, John P. Seymour, Paras R. Patel, Tim M. Bruns, Cynthia A. Chestek
Summary: The study introduced a novel and durable carbon fiber electrode array for precise neural recording, showing potential as a bioelectric medicine tool for diagnosis and closed-loop neural control of therapeutic treatments and monitoring systems.
IEEE TRANSACTIONS ON NEURAL SYSTEMS AND REHABILITATION ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Rene Y. Y. Lawong, Fabian May, Etang C. C. Etang, Philipp Vorrat, Jonas George, Julia Weder, Dagmar Kockler, Matthias Preller, Mike Althaus
Summary: The epithelial sodium channel (ENaC) is crucial for maintaining sodium homeostasis and blood pressure control. It is regulated by extracellular sodium ions through a mechanism called sodium self-inhibition (SSI). In this study, we evaluated the effectiveness of an automated two-electrode voltage-clamp system in detecting ENaC activity and SSI in Xenopus oocytes. Despite some limitations, the system was able to accurately detect the SSI characteristics of different ENaC orthologs and variants associated with hypertension.
Article
Chemistry, Multidisciplinary
Tomoaki Banno, Shuhei Tsuruhara, Yu Seikoba, Ryohei Tonai, Koji Yamashita, Shinnosuke Idogawa, Yuto Kita, Ko Suzuki, Yuki Yagi, Yuki Kondo, Rika Numano, Kowa Koida, Takeshi Kawano
Summary: Microscale needle-like electrodes offer high spatiotemporal resolution for in vivo extracellular recording. However, reducing the size to the nanoscale increases electrical impedance. By stacking a nanoelectrode on an amplifier module, the quality of neuronal signal recording can be improved. In experiments conducted on the mouse cortex, the nanoelectrode with amplifier could record a wider range of neural signals compared to the electrode without the amplifier.
Article
Biophysics
Venkata Suresh Vajrala, Kamil Elkhoury, Sophie Pautot, Christian Bergaud, Ali Maziz
Summary: This paper reports a novel Hollow Ring-like type electrode for sensing and/or stimulating neural activity from three-dimensional neural networks. The ring electrode architecture enables easy access to three-dimensional neural networks with reduced mechanical contact on the biological tissue, and provides improved electrical interface with cells. The hollow ring design shows great potential for developing next-generation microelectrodes for applications in neural interfaces.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Yuecheng Zhou, Erica Liu, Yang Yang, Felix S. Alfonso, Burhan Ahmed, Kenneth Nakasone, Csaba Forro, Holger Muller, Bianxiao Cui
Summary: Optical recording based on voltage-sensitive electrochromic materials allows for long-term and sensitive measurement of bioelectrical signals.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Materials Science, Multidisciplinary
Zhiyuan Chen, Khanh Nguyen, Grant Kowalik, Xinyu Shi, Jinbi Tian, Mitansh Doshi, Bridget R. R. Alber, Xun Guan, Xitong Liu, Xin Ning, Matthew W. W. Kay, Luyao Lu
Summary: Transparent microelectrodes have been widely studied in the biomedical field due to their advantages in simultaneous electrical and optical interrogation of cell/tissue activity. In this study, a microelectrode array (MEA) with gold-coated silver nanowires (Au-Ag NWs) was designed to achieve desirable mechanical stretchability, optical transparency, electrochemical performance, and chemical stability. The MEAs exhibited high optical transparency, low electrochemical impedance, stable chemical and electromechanical performance, and superior capability for colocalized electrophysiological and optical mapping of cardiac function.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Multidisciplinary Sciences
Jokubas Ausra, Micah Madrid, Rose T. Yin, Jessica Hanna, Suzanne Arnott, Jaclyn A. Brennan, Roberto Peralta, David Clausen, Jakob A. Bakall, Igor R. Efimov, Philipp Gutruf
Summary: Monitoring and control of cardiac function are crucial for studying cardiovascular pathophysiology and developing treatments. However, it is currently challenging to control the heart in freely moving small animal subjects. This study introduces a wireless battery-free device that enables real-time cardiac control and multisite stimulation, allowing optogenetic modulation of the entire rodent heart.
Article
Engineering, Biomedical
Kunal Sahasrabuddhe, Aamir A. Khan, Aditya P. Singh, Tyler M. Stern, Yeena Ng, Aleksandar Tadic, Peter Orel, Chris LaReau, Daniel Pouzzner, Kurtis Nishimura, Kevin M. Boergens, Sashank Shivakumar, Matthew S. Hopper, Bryan Kerr, Mina-Elraheb S. Hanna, Robert J. Edgington, Ingrid McNamara, Devin Fell, Peng Gao, Amir Babaie-Fishani, Sampsa Veijalainen, Alexander Klekachev, Alison M. Stuckey, Bert Luyssaert, Takashi D. Y. Kozai, Chong Xie, Vikash Gilja, Bart Dierickx, Yifan Kong, Malgorzata Straka, Harbaljit S. Sohal, Matthew R. Angle
Summary: The Argo system developed in this study allows for high-speed recording of neural activity in the cortex, with the highest channel count in vivo recording system currently available. Through bench testing and in vivo experiments, neural activities in the rat and sheep cortex were successfully recorded. This system has potential for clinical translation in the future.
JOURNAL OF NEURAL ENGINEERING
(2021)
Article
Neurosciences
Thomas Parr, Rajeev Vijay Rikhye, Michael M. Halassa, Karl J. Friston
Article
Multidisciplinary Sciences
Michael Douglas Reed, Yeong Shin Yim, Ralf D. Wimmer, Hyunju Kim, Changhyeon Ryu, Gwyneth Margaret Welch, Matias Andina, Hunter Oren King, Ari Waisman, Michael M. Halassa, Jun R. Huh, Gloria B. Choi
Review
Neurosciences
Miho Nakajima, L. Ian Schmitt
NEUROSCIENCE RESEARCH
(2020)
Article
Environmental Sciences
Marcos Mateus, Miguel Remondes, Ligia Pinto, Alexandra Silva
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Neurosciences
Marcelo Dias, Ines Marques-Morgado, Joana E. Coelho, Pedro Ruivo, Luisa Lopes, Miguel Remondes
Summary: A new method for transection and retraction of the superior sagittal sinus in rats is described, enabling direct access to brain areas previously obstructed by the sinus, without causing hemorrhage or adverse consequences.
Article
Neurosciences
Marcelo Dias, Raquel Ferreira, Miguel Remondes
Summary: The study reveals the crucial role of MEC excitatory neurons in timing behavior, suggesting these neurons contribute to such behavior by holding temporal information across trials. The silencing of MEC activity impairs the reproduction of memorized intervals and results in an overestimation of elapsed time. Additionally, decoding behavioral performance from preceding waiting times is compromised when MEC is silenced, indicating a key role of MEC in maintaining temporal memories across trials.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Arghya Mukherjee, Norman H. Lam, Ralf D. Wimmer, Michael M. Halassa
Summary: Interactions between the mediodorsal thalamus and the prefrontal cortex play a critical role in cognition. Two distinct mediodorsal projections to the prefrontal cortex have complementary mechanistic roles in decision-making under uncertainty. These mechanisms offer a potential entry point for correcting decision-making abnormalities in disorders with a prominent prefrontal component.
Review
Neurosciences
Dheeraj S. Roy, Ying Zhang, Michael M. Halassa, Guoping Feng
Summary: Recent studies using advanced expression profiling have revealed gene expression gradients within and across thalamic nuclei, redefining functional units of the thalamus. Thalamic subnetworks, defined by the intersection of genetics, connectivity, and computation, provide a more appropriate level of functional description.
NATURE NEUROSCIENCE
(2022)
Article
Computer Science, Artificial Intelligence
Xiaohe Xue, Ralf D. Wimmer, Michael M. Halassa, Zhe Sage Chen
Summary: The prefrontal cortical neurons are essential in rule-dependent tasks, and a neural network model can generate rule-specific tunings in single-unit representations similar to experimentally observed data. Through computational modeling, we can understand neuronal representations at a fine timescale during working memory and cognitive control.
COGNITIVE COMPUTATION
(2022)
Article
Neurosciences
Mien Brabeeba Wang, Michael M. Halassa
Summary: Animal brains evolved to optimize behavior in dynamic environments, selecting actions that maximize future rewards. This study explores how the brain achieves optimal wiring adjustments in the presence of ambiguous sensory inputs and rewards. The authors propose that the interaction between the cortex, thalamus, and basal ganglia provides a solution, with the thalamus playing a key role in meta-learning. Different control functions in the thalamus guide plasticity in the cortex across two timescales, enabling behavioral flexibility and generalization to new contexts.
NETWORK NEUROSCIENCE
(2022)
Article
Psychology, Experimental
Goncalo A. Oliveira, Miguel Remondes, Teresa Garcia-Marques
Summary: Conflict and perceptual disfluency can lead to adaptive control adjustments and these effects may be integrated into a general feeling of disfluent information processing. Results showed that increasing processing disfluency led to a decrease in the interference of incongruent fluent trials, and this effect was significant for all conditions of proportion of congruence, although it was lower when incongruent trials were more frequent. These findings highlight the role of feelings in initiating control and suggest that changes in information processing fluency may serve as a signal for the need for control.
PSYCHOLOGICAL RESEARCH-PSYCHOLOGISCHE FORSCHUNG
(2022)
Review
Clinical Neurology
Arghya Mukherjee, Michael M. Halassa
Summary: Schizophrenia is a brain disorder that affects cognitive processing. While its symptoms can be treated, there is a lack of effective methods to measure and address its impact on reasoning, inference, and decision making. Recent research has shown dysfunction in the interactions between the prefrontal cortex and thalamus in schizophrenia, and this can be understood in the context of neural circuit research in nonhuman animals. The thalamus plays a critical role in maintaining and switching prefrontal activity patterns in various cognitive tasks. Focusing on associative thalamic structures can help us better understand cognitive processing and develop diagnostic and therapeutic approaches for schizophrenia.
Article
Biochemical Research Methods
Ali Hummos, Bin A. Wang, Sabrina Drammis, Michael M. Halassa, Burkhard Pleger
Summary: Interactions across frontal cortex are critical for cognition. Recent research suggests that the mediodorsal thalamus (MD) plays a role in these interactions, but the specific computations and relevance to human decision making remain unclear. In this study, a neural model of an executive frontal-MD network was trained on a human decision-making task, and the results showed that the MD compressed its cortical inputs and efficiently partitioned cortical activity patterns, enhancing task switching. The findings contribute to the emerging evidence for thalamic regulation of frontal interactions in the human brain.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Psychology, Biological
Goncalo A. Oliveira, Miguel Remondes, Teresa Garcia-Marques
Summary: Recent research suggests that the cognitive monitoring system may use negative emotional cues to regulate information processing. However, this study proposes that positive feelings of ease-of-processing can hinder adaptive control adjustments. The study found that in mostly congruent contexts, participants made more errors on incongruent trials that were easy-to-read. Similarly, in mostly incongruent contexts, participants made more errors after experiencing the facilitation effect of repeated congruent trials. These findings highlight the importance of transient and sustained feelings of processing fluency in regulating control mechanisms.
QUARTERLY JOURNAL OF EXPERIMENTAL PSYCHOLOGY
(2023)
Article
Biology
Arghya Mukherjee, Navdeep Bajwa, Norman H. Lam, Cesar Porrero, Francisco Clasca, Michael M. Halassa