Article
Chemistry, Analytical
Mai Zhang, Honghong Wang, Hui Wang, Fangfang Wang, Zhengping Li
Summary: A CRISPR/Cas12a-assisted sequence-specific detection method for LAMP products was developed to eliminate non-specific amplification effects. By designing target-specific stem-loop DNA probes, primer design for LAMP was greatly simplified, achieving sensitive detection of miRNAs with high specificity.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Xiaowen Cao, Feng Chen, Jing Xue, Yue Zhao, Min Bai, Yongxi Zhao
Summary: Here, we present a hierarchical DNA branch assembly-encoded fluorescent nanoladders for denoised and highly multiplexed signal amplification of single-cell transcripts. Our method achieves independent RNA-primed rolling circle amplification without nonspecific amplification and allows for programmable DNA branch assembly to encode virtual signals for visualizing numbers of targets. We demonstrate accurate RNA profiling in different cancer cells and reveal diverse localization patterns for spatial regulation of transcripts.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Analytical
Yanfei Zhang, Jun Chen, Huihui Yang, Wen Yin, Chunrong Li, Yuzhi Xu, Si-Yang Liu, Zong Dai, Xiaoyong Zou
Summary: The LCR-HCR method uses exosomes as vehicles for controlled release and recruitment, significantly improving miRNA imaging contrast by confining reactants on exosome vehicles. It provides a powerful tool for sensitive miRNA imaging in living cells and cancer diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Juan Song, Shan Li, Jie Zhou, Qiao Yu, Xue-Jiao Yang, Hong-Yuan Chen, Jing-Juan Xu
Summary: We have developed a triple signal amplification strategy for in situ visualization of telomerase RNA (TR) in living cells. The strategy includes target-initiated strand displacement circuit, multidirectional rolling circle amplification (RCA), and Mg2+ DNAzyme-mediated amplification. The proposed method exhibited ultrasensitive detection of TR mimic with a detection limit at the picomolar level. Moreover, after transfection with the proposed sensing platform, tumor cells can be easily distinguished from normal cells based on TR abundance-related fluorescence signal, providing new insights into initial cancer screening.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Jun Zhao, Jiandong Wu, Xiaosong Wu, Guoqing Deng, Yong Liu, Francis Lin, Ling Zhu
Summary: An efficient self-quenching fluorescence probe and the corresponding detection system were designed, capable of achieving a wide dynamic range and low detection limit in a single step. The method, with high specificity and biocompatibility, has great potential for accurately quantifying miRNA biomarkers in serum, providing promising prospects for clinical diagnosis.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Masanobu Nagano, Takumi Toda, Kurumi Makino, Hiroko Miki, Yoshiaki Sugizaki, Hideyuki Tomizawa, Atsunobu Isobayashi, Keitaro Yoshimoto
Summary: Researchers have developed an MTX detection system using chemically synthesizable homogeneous oligonucleotides. Through microbead-assisted capillary electrophoresis, they successfully identified MSmt7 with a similar level of specificity to antiMTX antibodies. This oligonucleotide can be coupled with a peroxidase-like hemin-DNAzyme to construct a bifunctional MTX sensing method with a low detection limit.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Shu-An Hsieh, Danial Shamsaei, Derek R. Eitzmann, Jared L. Anderson
Summary: This study demonstrates the use of a 3D printed droplet generation platform coupled with molecular beacons in LAMP detection, allowing for low-cost and customizable droplet generation. The study also demonstrates the use of a smartphone-based imaging system to increase accessibility. This method can be used for sequence-specific, sensitive, and accurate DNA quantification.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Sidan Wang, Haojia Li, Kejun Dong, Wan Shu, Jiarui Zhang, Jun Zhang, Rong Zhao, Sitian Wei, Dilu Feng, Xianjin Xiao, Wei Zhang
Summary: Point of care testing (POCT) plays a crucial role in disease diagnosis and prognosis evaluation. The biosensor based on CRISPR/Cas12a is a powerful diagnostic tool due to its high sensitivity. However, its limited recognition ability and high cost hinder its universal application. Therefore, it is important to develop a CRISPR/Cas12a-based RNA detection system that is easy to use, cheap, specific, and universal.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Jingyang Zhang, Yu Wang, Xudong Zhao, Mengmeng Chen, Yuan Peng, Jialei Bai, Shuang Li, Dianpeng Han, Shuyue Ren, Kang Qin, Sen Li, Tie Han, Zhixian Gao
Summary: The development of a dual sensitization smartphone colorimetric strategy based on rolling circle amplification (RCA) coils gathering Au tetrahedra has been successful. This strategy utilizes the dual sensitization effect of RCA and Au tetrahedra to amplify the signal. The use of ImageJ software allows for timely target detection under practical conditions.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Hang Ao, Wencheng Xiao, Yuhui Chen, Jie Wu, Huangxian Ju
Summary: This study developed a target-triggered parallel rolling circle amplification circuit (PRCAC) strategy for chemiluminescent (CL) protein imaging assay. PRCAC generated G-quadruplex units to form DNAzyme, which catalyzed the oxidation of luminol by H2O2 to produce a strong CL signal. The method demonstrated low limits of detection, excellent specificity, simplicity, short analytical time, and reliability for biomarker analysis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Multidisciplinary Sciences
Gun-Soo Park, Jin-Soo Maeng
Summary: We developed a method for amplifying kilobases-long target sequence from RNA templates using Cre recombinase to generate circular intermediate template for subsequent amplification. The method can amplify a target sequence of about 2.9 kb, and the amplicon can be used for cloning.
SCIENTIFIC REPORTS
(2022)
Article
Biophysics
Dongdong Liu, Wenhua Li, Mingzhu Yang, Lizhen Qiu, Hongru Pian, Yongsheng Huang, Mian Chen, Zhi Zheng
Summary: By introducing Au-TPP and 'C' form target-specific oligonucleotide linker probes, a specific and photothermal mediated hyperbranched rolling circle amplification was successfully developed for direct in situ mRNA detection, suitable for both FFPE tissue section and frozen tissue section.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Ruolan Huang, Kun Liu, Chensirong Yue, Ying Wang, Zhilong Zeng, Lu Xiao, Lei Dong, Rongqin Ke, Chaoyong Yang, Dan Liu
Summary: This study developed a method for visualizing mRNA at single-molecule resolution using rolling circle amplification and streptavidin-modified alkaline phosphatase (SA-ALP) detection. The method provides highly selective and sensitive RNA detection, comparable to fluorescence-based assays. It also overcomes common background interference issues and allows for the quantification of mRNA expression from two genes in single cells and multiple cell types.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jinhua Shang, Shanshan Yu, Ruomeng Li, Yuqiu He, Yushi Wang, Fuan Wang
Summary: Rolling circle amplification (RCA) is used to construct compact and versatile DNA nanoassemblies, but it is rarely used for intracellular analysis due to uncontrollable and inefficient probe integration/activation. Here, we developed a multifunctional RCA nanogel encoded with tandem allosteric deoxyribozyme for efficient intracellular microRNA imaging. The endogenous microRNA stimulates the precise degradation of DNA nanocarriers, leading to the exposure of RCA-entrapped DNAzyme biocatalyst for amplified readout signal. Our bioorthogonal DNAzyme disassembly strategy enables robust analysis of intracellular biomolecules with potential applications in clinical diagnosis.
Article
Microbiology
Xuelian Rao, Liangkai Zheng, Kaipeng Wei, Meiqing Li, Meng Jiang, Jianlong Qiu, Yulin Zhou, Rongqin Ke, Chen Lin
Summary: In situ detection of viral mRNA expression in tissue samples is valuable for pathological diagnosis. However, conventional RNA in situ hybridization assays lack sensitivity and specificity for clinical purposes. This study developed a novel in situ hybridization assay for HPV E6/E7 mRNA, which allows visualization of mRNA as discrete dot-like signals using bright-field microscopy. The results are consistent with traditional staining methods and offer potential applications for clinical diagnostics.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Physical
Wenyan Yu, Cuiping Xuan, Bingbing Liu, Lei Zhou, Na Yin, Enpeng Gong, Zhenzhong Zhang, Yinchao Li, Kaixiang Zhang, Jinjin Shi
Summary: A carrier-free programmed spherical nucleic acid nanostructure has been developed, which showed efficient blood-brain barrier penetration capability and satisfactory caspase-3 silencing effect, significantly improving the therapeutic efficacy of ischemic stroke.
Article
Chemistry, Analytical
Yanan Li, Di Huang, Yiran Pei, Fenglei Quan, Hua Gao, Junli Zhang, Hongwei Hou, Kaixiang Zhang, Jinghong Li
Summary: Site-specific imaging of target genes using CRISPR probes is crucial for understanding gene function and developing tools for gene manipulation. The CasSABER probe allows programmable in situ imaging of low and nonrepetitive regions of a gene. It can specifically light up gene loci bound by dCas9/sgRNA, enabling high-specificity and high-signal-to-noise ratio imaging of fixed cellular gene loci. The CasSABER system also enables adjustable signal amplification, allowing simultaneous visualization of multicopy and single-copy gene loci with similar fluorescence intensity.
ANALYTICAL CHEMISTRY
(2023)
Review
Engineering, Biomedical
Danyu Wang, Jie Duan, Jingwen Liu, Hua Yi, Zhenzhong Zhang, Haiwei Song, Yinchao Li, Kaixiang Zhang
Summary: DNA hydrogels are increasingly important in biomedical and bioanalysis applications. They are highly programmable, multifunctional, and biocompatible, making them effective carriers for drugs and bioactive cargoes. However, the stability of DNA hydrogels hinders rapid release of cargoes. Stimulus-responsive DNA hydrogels have been designed to meet these requirements, switching from gel to solution for precision cargo delivery and bioanalysis. This review summarizes innovative methods for designing stimuli-responsive self-degradable DNA hydrogels and discusses their applications and challenges in the fields of bioanalysis and biomedicine.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Junli Zhang, Mengting Guan, Chihong Ma, Yingying Liu, Min Lv, Zhenzhong Zhang, Hua Gao, Kaixiang Zhang
Summary: Exosomal miRNAs have a critical role in cancer biology and may serve as potential biomarkers for cancer diagnosis. However, detecting disease-associated exosomal miRNAs in an easy-to-operate manner is challenging due to their low abundance. In this study, a liposome-mediated membrane fusion strategy (MFS) called MFS-CRISPR was used to directly measure exosomal miRNAs in plasma. The MFS-CRISPR platform exhibited a linear detection range from 10(4) to 10(8) particles/mL and could detect exosomal miR-21 at a low concentration of 1.2 x 10(3) particles/mL. The proposed method showed promising clinical potential for cancer diagnosis and treatment monitoring due to its high sensitivity and simplicity.
Article
Chemistry, Multidisciplinary
Yan Liang, Wenshuai Han, Chenlu Xu, Jinjin Wang, Jingge Zhang, Jingyi An, Wei Liu, Junjie Liu, Zhenzhong Zhang, Jinjin Shi, Kaixiang Zhang
Summary: A cascade-responsive oxidative stress amplifier, DR-TAF-pHT/FA, is proposed that can generate reactive oxygen species (ROS) on the lysosomal membrane of tumor cells and release Cas9/sgNrf2 RNPs for efficient gene disruption. The treatment significantly improves tumor ablation efficiency and extends median survival time in xenograft models.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Shizhen Geng, Tingting Xiang, Yunya Zhang, Pengke Guo, Hongling Zhang, Zhenzhong Zhang, Mengchao Gu, Kaixiang Zhang, Haiwei Song, Jinjin Shi, Junjie Liu
Summary: Abundant CAFs in highly fibrotic breast cancer act as an immunosuppressive barrier and are linked to the failure of ICB. To overcome this, a strategy to transform immune-suppressed CAFs into immune-activated APCs is proposed. A photo-controlled gene expression nanosystem is developed to engineer CAFs as APCs and induce activation and proliferation of CD8+ T cells.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Chemistry, Multidisciplinary
Xianglin Zhu, Kaixiang Zhang, Xucong Teng, Xuhan Xia, Ruijie Deng, Jinghong Li
Summary: The gut microbiota is an important hidden organ closely related to human health. Emerging techniques such as spatially resolved sequencing and in vivo imaging enable the acquisition of spatial organizations and dynamic information of the gut microbiome, providing new insights into physiological activities, symbiotic functions, and spatial organization rearrangement under different physiological and pathological conditions, which have implications for diet and clinical treatment through gut microbiota management.
Article
Chemistry, Multidisciplinary
Xiu Zhao, Hui Cheng, Qiongwei Wang, Weimin Nie, Yue Yang, Xinyuan Yang, Kaixiang Zhang, Jinjin Shi, Junjie Liu
Summary: This study reports a photosensitizer metabolism-regulating strategy using ALA/DNAzyme-co-loaded nanoparticles (ALA & Dz@ZIF-PEG) for mitochondria-targeting photodynamic immunotherapy. The nanoparticles release zinc ions (Zn2+) in tumor cells, which promotes the conversion of ALA to PpIX and enhances the accumulation of PpIX in mitochondria, leading to improved photodynamic therapy outcomes.
Article
Chemistry, Multidisciplinary
Danyu Wang, Hua Yi, Shizhen Geng, Chuanmei Jiang, Jingwen Liu, Jie Duan, Zhenzhong Zhang, Jinjin Shi, Haiwei Song, Zhenzhen Guo, Kaixiang Zhang
Summary: Multidrug resistance (MDR) is a major cause of chemotherapy failure in oncology, and gene therapy is an excellent measure to reverse MDR. However, conventional gene therapy only modulates the expression of MDR-associated proteins but hardly affects their existing function, thus limiting the efficiency of tumor treatment. Therefore, we designed a photoactivated DNA nanodrug (MCD@TMPyP4@DOX) to improve tumor chemosensitivity through the downregulation of MDR-related genes and mitochondria-targeted photodynamic therapy (PDT).
Article
Chemistry, Multidisciplinary
Junli Zhang, Mengting Guan, Min Lv, Yingying Liu, Hongling Zhang, Zhenzhong Zhang, Kaixiang Zhang
Summary: In this study, a new method (LITIE) for localized imaging of tumor-derived EVs PD-L1 was developed, which can predict the response to immunotherapy. The method effectively distinguished breast cancer patients from healthy individuals or patients with benign tumors. The results also showed that the method could predict the effectiveness of immunotherapy before drug treatment.
Article
Chemistry, Multidisciplinary
Yan Liang, Jingge Zhang, Chenlu Xu, Jinjin Wang, Wenshuai Han, Jiali Yang, Sixuan Wu, Jingyi An, Junjie Liu, Zhenzhong Zhang, Jinjin Shi, Kaixiang Zhang
Summary: The researchers have developed an all-in-one biomimetic mineralized CRISPR/Cas9 RNA delivery system, which achieves efficient multiplex gene editing by editing multiple genomic loci. The system allows for precise control over the coencapsulation ratio between Cas9 mRNA and multiple sgRNAs, with a high RNA loading capacity. It also enhances the storage stability of RNA at 4°C and enables precise targeting of RNA nanoparticles in vivo. The system successfully achieved significant gene editing and inhibited tumor growth without off-target effects in liver tissue.
Correction
Chemistry, Multidisciplinary
Jingyi An, Kaixiang Zhang, Binghua Wang, Sixuan Wu, Yifei Wang, Hongling Zhang, Zhenzhong Zhang, Junjie Liu, Jinjin Shi
Article
Multidisciplinary Sciences
Danyu Wang, Jingwen Liu, Jie Duan, Hua Yi, Junjie Liu, Haiwei Song, Zhenzhong Zhang, Jinjin Shi, Kaixiang Zhang
Summary: The authors developed a DNA hydrogel that can monitor tumor recurrence and prevent postoperative tumor recurrence and metastasis. The hydrogel contains PDL1 aptamers to capture and enrich relapsed tumor cells, increasing local ATP concentration and providing a timely warning signal. By triggering photodynamic therapy and releasing tumor-associated antigens, as well as promoting systemic antitumor immunotherapy, the hydrogel system effectively inhibits recurrent tumors and suppresses metastasis.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Yanan Li, Ru Xu, Yonghua Wu, Jialing Guo, Fenglei Quan, Yiran Pei, Di Huang, Xiu Zhao, Hua Gao, Junjie Liu, Zhenzhong Zhang, Jinjin Shi, Kaixiang Zhang
Summary: This study presents a mutation-induced drug release system (MIDRS) that utilizes the single-nucleotide variation (SNV) recognition ability and trans-cleavage activity of Cas12a to convert tumor-specific mtDNA mutations into a regulatory switch for intracellular drug release, achieving precise tumor cell killing. The MIDRS system demonstrated excellent antitumor effects without affecting normal cells, leading to a stronger systemic antitumor immune response. This strategy shows promise for mutation-specific personalized tumor treatment approaches.
Correction
Chemistry, Multidisciplinary
Xiu Zhao, Hui Cheng, Qiongwei Wang, Weimin Nie, Yue Yang, Xinyuan Yang, Kaixiang Zhang, Jinjin Shi, Junjie Liu
Article
Chemistry, Multidisciplinary
Alexander M. Prophet, Kritanjan Polley, Gary J. Van Berkel, David T. Limmer, Kevin R. Wilson
Summary: The oxidation kinetics of iodide by ozone at the air-water interface is studied in single microdroplets. Molecular simulations and kinetic modeling are used to understand the underlying multiphase mechanism.
Article
Chemistry, Multidisciplinary
Rong Zhang, Jiajing Lan, Fei Wang, Shumei Chen, Jian Zhang
Summary: By utilizing 1,1'-ferrocene dicarboxylic acid as a chelating and surface protection ligand, we have synthesized multi-nuclear indium oxide clusters with varying nuclear sizes, including heptanuclear and thirteen-nuclear clusters. These clusters possess labile coordination sites, allowing for structural modification and self-assembly, resulting in the formation of various cluster structures.
Article
Chemistry, Multidisciplinary
Hui Wang, Hui Liu, Mingsen Wang, Jiaheng Hou, Yongjun Li, Yuancheng Wang, Yingjie Zhao
Summary: Two supramolecular complexes were prepared using CBs and M1, and their single-crystal structures were analyzed by SCXRD. The unexpected 1:2 self-assembly structure between M1 and CB[8] was discovered for the first time. These complexes exhibit unique photophysical properties and provide valuable information about the structure and photophysical properties of supramolecular complexes.
Article
Chemistry, Multidisciplinary
Chang-Hee Lee, Sookil Park, Sanggil Kim, Ji Young Hyun, Hyun Soo Lee, Injae Shin
Summary: The epidermal growth factor receptor (EGFR) is a cell-surface glycoprotein involved in cell proliferation and tumor development. This study used a fluorescently labeled EGFR to investigate its time-dependent endocytosis in live cells and found that appended glycans affect EGFR internalization. Additionally, the study detected sialic acid residues attached to EGFR on the live cell surface using FRET-based imaging. This research provides valuable insights into the cellular functions of EGFR.
Article
Chemistry, Multidisciplinary
Subhankar Sahu, Lokesh Kumar, Sumita Das, Dipti Gupta, Ruchi Anand
Summary: This study proposes a strategy that combines organic electronics with biosensor scaffolds to create a compact device for monitoring environmental aromatic pollution. By coupling biosensing protein MopR with an organic electrochemical transistor (OECT), a sensor module capable of efficient detection of phenol was designed. Exclusive phenol detection with minimal loss of sensitivity could be achieved in complex pollutant mixtures and real environmental samples.
Review
Chemistry, Multidisciplinary
Changseok Lee, Hyung-Joon Kang, Sungwoo Hong
Summary: The formation of C-N bonds through hydroamination reactions catalyzed by nickel hydrides has been a topic of recent interest. This approach offers a way to efficiently transform a variety of alkene and alkyne substrates into compounds enriched with C-N bonds. The review provides a concise overview of the underlying reaction mechanisms and aims to stimulate further progress in NiH-catalytic techniques and catalyst design.
Article
Chemistry, Multidisciplinary
Yueci Wu, Lu-Lu Sun, Hai-Hao Han, Xiao-Peng He, Weiguo Cao, Tony D. James
Summary: Drug-induced liver injury (DILI) is a common cause of acute liver failure in the USA and Europe, but most cases can be recovered or prevented by discontinuing the offending drug. Recent research has found that peroxynitrite (ONOO-) can be used as a potential indicator for early diagnosis of DILI, and there is an urgent need to establish a method to detect and track peroxynitrite in DILI cases. In this study, a FRET-based nano fluorescent probe CD-N-I was developed, which showed high selectivity and sensitivity in detecting peroxynitrite. The probe successfully detected exogenous peroxynitrite in live cells and endogenous peroxynitrite in APAP-induced liver injury of HepG2 cells.
Article
Chemistry, Multidisciplinary
Dmitry L. Lipilin, Mikhail O. Zubkov, Mikhail D. Kosobokov, Alexander D. Dilman
Summary: This article describes a direct photocatalytic method for the thiolation of unprotected acids, which was previously challenging. By using a thionocarbonate reagent with an N-O bond, the efficient conversion of carboxylic acids to thiols is achieved.
Article
Chemistry, Multidisciplinary
Jason Malenfant, Lucille Kuster, Yohann Gagne, Kouassi Signo, Maxime Denis, Sylvain Canesi, Mathieu Frenette
Summary: Raman microscopy can reveal compound-specific vibrational fingerprints without sample preparation. The combination of efficient theoretical calculations and a user-friendly software can accurately predict peak positions and provide match scores to assist with structure determination.
Article
Chemistry, Multidisciplinary
Jayoh A. Hernandez, Paul S. Micus, Sean Alec Lois Sunga, Luca Mazzei, Stefano Ciurli, Gabriele Meloni
Summary: Essential trace metals play crucial roles in the survival and virulence of bacterial pathogens. Helicobacter pylori requires nickel for colonization and persistence in the stomach, and NixA is an essential nickel transporter in this process. This study characterizes the selectivity and electrogenic nature of NixA-mediated nickel transport.
Article
Chemistry, Multidisciplinary
Tarali Devi, Kuheli Dutta, Jennifer Deutscher, Stefan Mebs, Uwe Kuhlmann, Michael Haumann, Beatrice Cula, Holger Dau, Peter Hildebrandt, Kallol Ray
Summary: This study emphasizes the importance of subtle electronic changes and secondary interactions in the stability of biologically relevant metal-dioxygen intermediates. It also shows that the role of the chloride ligand in stabilizing the Fe-III-(OOBu)-Bu-t moiety can extend to other anions, including the thiolate ligand.
Article
Chemistry, Multidisciplinary
Jacqueline R. Santhouse, Jeremy M. G. Leung, Lillian T. Chong, W. Seth Horne
Summary: By studying the folding kinetics and mechanism of the BdpA sequence, researchers found that altering the backbone connectivity can affect protein folding. This suggests that protein mimetic chains have a significant degree of plasticity in transitioning between unfolded and folded states.
Article
Chemistry, Multidisciplinary
Divanshu Gupta, Ralf Einholz, Holger F. Bettinger
Summary: This study presents the first direct spectroscopic evidence of a cyclic seven-membered iminoborane. Compared to linear amino-iminoboranes, this cyclic iminoborane exhibits weakened bond strength and lower Lewis acidity value. The study suggests that the reduced ring strain of cyclic iminoborane prevents nitrogen fixation but allows facile (2 + 2) cycloaddition reaction with C2H4.
Article
Chemistry, Multidisciplinary
Renny Mathew, Aniruddha Mazumder, Praveen Kumar, Julie Matula, Sharmarke Mohamed, Petr Brazda, Mahesh Hariharan, Brijith Thomas
Summary: This study reveals the packing arrangement of partially disordered nitro-perylenediimide (NO2-PDI) using a synergistic approach that combines 3D ED, ssNMR, and DFT techniques. By overcoming these challenges, this methodology opens up new avenues for material characterization, driving exciting advancements in the field.
