Article
Plant Sciences
Chao Xi, Mingyue Zhang, Bingtao Li, Xiaowei Meng, Shangcheng Xu, Hong Du, Xinyi Wang, Jingping Xu, Hong Ke, Yanru Cui, Fei Qu
Summary: The anti-inflammatory effects of Pueraria lobata and Pueraria thomsonii have been confirmed, and their mechanism of action involves regulating arachidonic acid metabolism and glutathione metabolism. These findings provide a scientific basis for the development of new drugs for inflammation-related diseases and lay a foundation for the utilization of herbal resources such as Pueraria lobata and Pueraria thomsonii.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Plant Sciences
Song Wang, Wei Yao, Xudong Zhu, Jingjing Wang, Longhui Lu, Na Zhu, Tong Lan, Yunxia Kuang, Weifeng Zhu, Ronghua Liu, Liping Huang
Summary: The antithrombotic mechanism of Pueraria lobata and Pueraria lobata var. thomsonii has been further clarified in this study. The study identified the active ingredients of the two plants and confirmed their antithrombotic effects through animal experiments and network pharmacology methods. The results showed that Pueraria lobata and Pueraria lobata var. thomsonii can reduce thrombus weight and platelet accumulation, inhibit coagulation indices, and regulate various physiological processes to achieve antithrombotic effects.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dan Gao, Jin Hyeok Kim, Cheong Taek Kim, Won Seok Jeong, Hyung Min Kim, Jaehoon Sim, Jong Seong Kang
Summary: The study revealed that the stem extract of Pueraria lobata has the potential to inhibit melanin production, with its main components puerarin and daidzin showing excellent binding affinities for human tyrosinase, suggesting its use as an anti-pigmentation agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Junping Liu, Jiayan Zhang, Tao Liao, Lei Zhou, Liqiang Zou, Yafei Liu, Li Zhang, Wei Liu
Summary: The study found that PPO in kudzu exhibited higher thermostability in food systems, with different food constituents varying in their effects on PPO. Among them, pectin showed the most significant protective effect against thermal inactivation.
Article
Materials Science, Biomaterials
Yumin Fan, Gangliang Huang
Summary: Pueraria lobata polysaccharides (PLPs) were obtained through a hot water extraction method. The extraction process was optimized using response surface methodology, and the optimal extraction parameters were determined to be an extraction temperature of 84°C, liquid-solid ratio of 11 mL/g, extraction time of 73 min, and a polysaccharide extraction rate of 8.59%. The PLPs were then purified by removing water-soluble proteins using the Sevag method, removing pigments using H2O2, and precipitation with anhydrous ethanol. Dialysis was used to remove soluble salts and small molecules, and the refined PLPs were obtained through freeze-drying.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Anami Ahuja, Pankaj Kumar Tyagi, Shruti Tyagi, Anuj Kumar, Manoj Kumar, Javad Sharifi-Rad
Summary: The study identified five phytochemicals from Pueraria tuberosa with strong BACE1 inhibition, which significantly improved cognitive decline in AD flies, suggesting that Pueraria tuberosa may be a promising dietary supplement for ameliorating cognitive decline caused by BACE1 in Alzheimer's disease.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Shifan Zhao, Hualei Xue, Yijiong Tao, Kai Chen, Xiao Li, Mi Wang
Summary: A water-soluble acidic heteropolysaccharide, named PPL-1, was isolated from Pueraria lobata. PPL-1 had an average molecular weight of 35 kDa and consisted of glucose, arabinose, galactose, and galacturonic acid. Methylation and NMR analysis revealed the linkage patterns of various sugar residues in PPL-1. PPL-1 exhibited strong DPPH radical scavenging activity and moderate proliferation enhancement effect on RAW 264.7 cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Weiqiang Zhan, Mingzhu Deng, Xinqia Huang, Dong Xie, Xiang Gao, Jiaxian Chen, Zhen Shi, Jiaxu Lu, Hao Lin, Peng Li
Summary: This study found that Pueraria lobata-derived exosome-like nanovesicles (PELNs) can promote osteoblast differentiation and mineralization in osteoporosis patients. Additionally, PELNs can reduce the relative abundance of harmful strains by regulating the metabolite TMAO produced by gut microbiota. The elevated autophagy levels achieved by degrading TMAO also contribute to the differentiation and function of hBMSCs.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Plant Sciences
Mei Fu, Mohammad Shah Jahan, Kang Tang, Shizheng Jiang, Juxian Guo, Shanwei Luo, Wenlong Luo, Guihua Li
Summary: In this study, the flavonoid, dietary fiber, total starch, and crude protein contents of Pueraria thomsonii and Pueraria lobata were compared. The results showed that P. lobata had higher levels of isoflavones and flavonoids compared to P. thomsonii. On the other hand, P. thomsonii had higher levels of total starch but lower levels of soluble dietary fiber, insoluble dietary fiber, and crude protein compared to P. lobata. These findings suggest that P. lobata is more suitable for medicinal use, while P. thomsonii is more suitable as an edible food, and provide a theoretical basis for the development of P. thomsonii and P. lobata germplasm resources.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jiaqi Wu, Xiaoyu Ma, Yu Lu, Tao Zhang, Zuoqin Du, Jin Xu, Jingcan You, Ni Chen, Xin Deng, Jianbo Wu
Summary: Pueraria lobata exerts anti-inflammatory effects through the activation of exosomes and shifts in macrophage polarization.
Article
Nutrition & Dietetics
Zhujun Wang, Hui Du, Wanqian Peng, Shilin Yang, Yulin Feng, Hui Ouyang, Weifeng Zhu, Ronghua Liu
Summary: This study investigates the effects of Pueraria polysaccharides on type 2 diabetes and its related mechanisms. The results show that Pueraria polysaccharides improve the metabolic profile of diabetic mice by regulating gut microbiota and metabolic pathways.
Article
Biochemistry & Molecular Biology
Qiang-Song Wang, Yi-Lun Wang, Wen-Yan Zhang, Kuang-Dai Li, Xiong-Fei Luo, Yuan-Lu Cui
Summary: IBS, especially IBS-D, affects approximately 9-20% of the global population, with traditional treatments often causing side effects. Natural medicines, such as puerarin, have gained attention for their beneficial effects in recent years. Puerarin has been found to reverse IBS-D symptoms by regulating gut microbiota and various physiological processes.
Article
Plant Sciences
Punam Thapa, Hye Mi Kim, Joon-Pyo Hong, Ranhee Kim, Sunil Babu Paudel, Hyukjae Choi, Dae Sik Jang, Joo-Won Nam
Summary: Pueraria lobata is a widely used medicinal plant in Korea, China, and Japan, containing bioactive substances in its flowers. This study developed a quantitative analysis of the isoflavones in Puerariae Flos using qHNMR spectroscopy, which was validated by HPLC-UV. The findings suggest that qHNMR spectroscopy is a reliable method for quantifying and standardizing isoflavone content in Puerariae Flos.
Article
Biochemistry & Molecular Biology
Ying-Jie Sun, Shi-Jie Cao, Feng-Ni Liang, Jin-Yan Li, Xiang-Yu Zhang, Wei Li, Li-Qin Ding, Feng Qiu
Summary: Five undescribed puerols and puerosides, along with three known analogues, were isolated from the roots of Pueraria lobata. Their structures were determined through comprehensive analysis of spectroscopic data and chemical methods. The absolute configurations of racemic compounds puerol D and puerol C were determined using experimental and calculated ECD spectra. The inhibitory activities of six isolates on NO generation and expression of inflammatory factors were evaluated, with (S)-puerol C, (R)-puerol C, isokuzubutenolide A, and kuzubutenolide A showing significant inhibitory effects.
Article
Plant Sciences
Yejin Kim, Jaeyoon Kim, So-Ri Son, Ji-Young Kim, Jung-Hye Choi, Dae Sik Jang
Summary: The extract of Puerariae Flos showed significant activity against human ovarian cancer cells in a preliminary study. This research aimed to identify the active compounds in Pueraria Flos and found several compounds that demonstrated cytotoxicity against the cancer cells.