期刊
VIRUSES-BASEL
卷 9, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/v9040092
关键词
citrus sudden death; CSDaV; CTV; plant viruses; high-throughput sequencing
类别
资金
- National Institute of Science and Technology of Genomics for Citrus Improvement (INCT Citros)
- National Council for Scientific and Technological Development [306230/2013-5, 2008/57909-2]
- Fundacao de Apoio a Pesquisa no Estado de Sao Paulo [2008/57909-2]
- National Council for Scientific and Technological Development (CNPq) fellowship
- Doctoral Sandwich Program Abroad (PDSE/CAPES) fellowship
Citrus sudden death (CSD) has caused the death of approximately four million orange trees in a very important citrus region in Brazil. Although its etiology is still not completely clear, symptoms and distribution of affected plants indicate a viral disease. In a search for viruses associated with CSD, we have performed a comparative high-throughput sequencing analysis of the transcriptome and small RNAs from CSD-symptomatic and -asymptomatic plants using the Illumina platform. The data revealed mixed infections that included Citrus tristeza virus (CTV) as the most predominant virus, followed by the Citrus sudden death-associated virus (CSDaV), Citrus endogenous pararetrovirus (CitPRV) and two putative novel viruses tentatively named Citrus jingmen-like virus (CJLV), and Citrus virga-like virus (CVLV). The deep sequencing analyses were sensitive enough to differentiate two genotypes of both viruses previously associated with CSD-affected plants: CTV and CSDaV. Our data also showed a putative association of the CSD-symptomatic plants with a specific CSDaV genotype and a likely association with CitPRV as well, whereas the two putative novel viruses showed to be more associated with CSD-asymptomatic plants. This is the first high-throughput sequencing-based study of the viral sequences present in CSD-affected citrus plants, and generated valuable information for further CSD studies.
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