Article
Virology
Syeda Sumera Naqvi, Nazish Bostan, Katsuhiko Fukai, Qurban Ali, Kazuki Morioka, Tatsuya Nishi, Muhammad Abubakar, Zaheer Ahmed, Sadia Sattar, Sundus Javed, Aamira Tariq, Asma Sadiq
Summary: This study reconstructed the evolutionary history of FMDV serotype A and one of its sub-lineages prevalent in Pakistan. Recombination patterns and natural selection acting on the serotype A genomes were estimated. Source and transmission routes in Pakistan were inferred.
Article
Virology
Ming Yang, Dmytro Zhmendak, Valerie Mioulet, Donald P. King, Alison Burman, Charles K. Nfon
Summary: Foot-and-mouth disease virus (FMDV) is a highly contagious virus that causes a devastating disease in cloven-hoofed animals. We have developed a rapid lateral flow strip test (LFST) that can detect FMDV, which is comparable to current laboratory methods in terms of sensitivity and specificity. This LFST can be used for on-site detection in endemic countries with limited laboratory resources.
Article
Immunology
W. A. Gayan Chathuranga, Chamith Hewawaduge, N. A. Nadeeka Nethmini, Tae-Hwan Kim, Ju Hun Kim, Young-Hoon Ahn, In-Joong Yoon, Sung-Sik Yoo, Jong-Hyeon Park, Jong-Soo Lee
Summary: This study developed two multiepitope recombinant proteins as potential vaccine candidates for foot-and-mouth disease. The results showed that these proteins effectively induced immune responses and protected animals from the virus. By using appropriate adjuvants, the protective efficacy of the vaccine candidates can be enhanced, providing a method for developing effective and safe vaccines for the prevention and control of foot-and-mouth disease.
Article
Veterinary Sciences
Yan Xiao, Suling Zhang, He Yan, Xiaolin Geng, Yanwei Wang, Xin Xu, Mengyue Wang, Haohao Zhang, Baicheng Huang, Wenqiang Pang, Ming Yang, Kegong Tian
Summary: The study successfully expressed capsid proteins of FMDV serotype O in Escherichia coli with an optimal tandem permutation (VP0-VP3-VP1), showing a protein yield close to 1:1:1. Immunization of pigs with FMDV VLPs induced FMDV-specific immune responses effectively and dose-dependently. The stable FMDV VLPs with multiple epitope exposure were effective for the induction of an immune response in pigs, laying a foundation for further development of the FMDV subunit vaccine.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Virology
Sasmita Upadhyaya, Mana Mahapatra, Valerie Mioulet, Satya Parida
Summary: Foot and mouth disease (FMD) is highly contagious and endemic in Southeast Asia (SEA) and East Asia (EA) with multiple circulating serotypes, posing a threat to countries like Australia. Current vaccines may not provide sufficient cross-protection, calling for continuous monitoring and potential development of new vaccine strains to address antigenic drift.
Article
Immunology
Ji-Hyeon Hwang, Gyeongmin Lee, Aro Kim, Jong-Hyeon Park, Min Ja Lee, Byounghan Kim, Su-Mi Kim
Summary: The newly developed A/SKR/Yeoncheon/2017 (A-1) vaccine strain shows high immunogenicity against FMDV serotype A in Asia and demonstrates good serological matching with field isolates in various tests.
Review
Virology
Qian Li, Ashenafi Kiros Wubshet, Yang Wang, Livio Heath, Jie Zhang
Summary: The failure of cross-protection among different serotypes of foot-and-mouth disease virus poses a significant threat to endemic countries. To address this, the development of a multi-epitope vaccine is considered a promising approach. In this review, we compile relevant bioinformatic reports on B and T cell epitopes of the SAT2 serotype of FMDV, as well as experimental demonstrations of vaccines designed against this serotype.
Article
Immunology
Ntungufhadzeni M. Rathogwa, Katherine A. Scott, Pamela Opperman, Jacques Theron, Francois F. Maree
Summary: This study compared the efficacy of two adjuvant formulations combined with a structurally stabilized SAT2 vaccine antigen and found that the Montanide ISA 206B-adjuvanted vaccine elicited a higher SAT2 neutralizing antibody response and three times higher systemic IFN-gamma responses compared to the Quil-A Saponin-adjuvanted vaccine group at 14- and 28-days post-vaccination. Serum antibodies from the immunized animals reacted similarly to both the parental vaccine virus and mutated viruses simulating antigenic drift in nature.
Article
Immunology
Ah-Young Kim, Hyejin Kim, Sun Young Park, Sang Hyun Park, Jae-Seok Kim, Jung-Won Park, Jong-Hyeon Park, Young-Joon Ko
Summary: The optimal stabilizer composition for long-term storage of FMD vaccine antigens, especially the O JC strain, was determined, extending shelf-life at both 4 degrees C and -70 degrees C.
Article
Virology
Hu Dong, Yuanlu Lu, Yun Zhang, Suyu Mu, Nan Wang, Ping Du, Xiaoying Zhi, Xiaobo Wen, Xiangxi Wang, Shiqi Sun, Yanming Zhang, Huichen Guo
Summary: This study identified thermostable FMDV mutants by high-temperature screening, with an important alanine-to-threonine mutation at position 13 in the VP1 protein for capsid stability. These mutants showed improved stability and immunogenicity compared to the wild-type virus, potentially aiding in the development of a stable vaccine against FMDV serotype O.
JOURNAL OF VIROLOGY
(2021)
Article
Immunology
Wanhong Xu, Ming Yang
Summary: Foot-and-mouth disease (FMD) is a severe and highly contagious viral disease, with vaccines playing a key role in disease control. The diversity of FMDV poses a huge challenge in selecting appropriate vaccines. Continuous monitoring of antigenic evolution is crucial for effective vaccine selection in combating FMD.
Review
Virology
David J. Paton, Antonello Di Nardo, Nick J. Knowles, Jemma Wadsworth, Edviges M. Pituco, Ottorino Cosivi, Alejandro M. Rivera, Labib Bakkali Kassimi, Emiliana Brocchi, Kris de Clercq, Consuelo Carrillo, Francois F. Maree, Raj K. Singh, Wilna Vosloo, Min-Kyung Park, Keith J. Sumption, Anna B. Ludi, Donald P. King
Summary: Foot-and-mouth disease is a highly contagious animal disease caused by an RNA virus. The serotype C strain has disappeared in many regions but has been recently detected, with cases closely related to vaccine strains. It is recommended to strengthen surveillance in FMD-endemic regions and to stop vaccination against serotype C to prevent its reappearance.
Article
Infectious Diseases
Da-Rae Lim, Soyoon Ryoo, Hyeonjeong Kang, Su Hong Oh, Sang-Ho Jang, BoKyu Kang, Hye-Jin Park, Hyeonwoo Hwang, Jae-Myung Kim, Choi-Kyu Park, Sang-Ho Cha
Summary: This study developed a multiplex real-time RT-PCR assay for specific detection and differential serotyping of foot-and-mouth disease virus. The assay demonstrated improved sensitivity and accuracy compared to current methods, and showed potential for enhancing the detection and serotyping capabilities of the virus in field samples.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Veterinary Sciences
Smrutirekha Mallick, Rabindra Prasad Singh, Jitendra Kumar Biswal, Jajati Keshari Mohapatra, Manoranjan Rout, Reshma Samanta, Sagar Ashok Khulape, Rajeev Ranjan
Summary: In this study, eight monoclonal antibodies (MAbs) were developed against the FMDV serotype O Indian vaccine strain. Three of these antibodies showed virus neutralizing activity. The MAb-based ELISA developed here exhibited high sensitivity and specificity for detecting FMDV serotype O antigen.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Virology
Kun Li, Yong He, Li Wang, Pinghua Li, Sheng Wang, Pu Sun, Huifang Bao, Yimei Cao, Xuerong Liu, Guoqiang Zhu, Yali Song, Xingwen Bai, Xueqing Ma, Yuanfang Fu, Hong Yuan, Jing Zhang, Jian Wang, Yingli Chen, Dong Li, Zhiyong Lou, Zaixin Liu, Zengjun Lu
Summary: Foot-and-mouth disease virus (FMDV) is a highly contagious virus that infects cloven-hoofed animals. In this study, researchers identified two cross-protective antigen sites on VP2 and VP3 using bovine-derived broadly neutralizing monoclonal antibodies. One site on VP3 was recognized by bnAb C4, while the other site on VP2 was recognized by 9 different bnAbs.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Jolene Carlson, Melina Fischer, Laura Zani, Michael Eschbaumer, Walter Fuchs, Thomas Mettenleiter, Martin Beer, Sandra Blome
Article
Biotechnology & Applied Microbiology
Martina Zimmermann, Melanie Nguyen, Christian M. Schultheiss, Harald Kolmar, Aline Zimmer
Summary: ThioFuc was investigated as a supplement to modulate the fucosylation profile of therapeutic proteins, resulting in enhanced effector functions. The efficient reduction of core-fucosylation by ThioFuc treatment led to increased afucosylation levels and improved Fc gamma RIIIa binding, ultimately enhancing ADCC efficacy. ThioFuc shows potential applications in drug development processes for improving therapeutic antibody functions.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Corinna Schmidt, Maria Wehsling, Maxime Le Mignon, Gregor Wille, Yannick Rey, Alisa Schnellbaecher, Dmitry Zabezhinsky, Markus Fischer, Aline Zimmer
Summary: This study investigated highly soluble and bioavailable derivatives of leucine and isoleucine for cell culture media, showing benefits in increasing overall solubility. The modified amino acids were found to be suitable for CHO cells and could replace canonical amino acids in cell culture feeds. Overall, N-lactoyl amino acids provide a promising alternative for developing highly concentrated cell culture media formulations to support next generation bioprocesses.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Christine H. Weiss, Corinna Merkel, Aline Zimmer
Summary: The composition of cell culture medium significantly impacts cell growth and quality attributes of proteins. This study specifically focused on the effects of different iron sources on cell line performance and glycosylation patterns, finding that impurities such as manganese can have a greater influence than iron itself.
BIOTECHNOLOGY PROGRESS
(2021)
Article
Biochemistry & Molecular Biology
Alisa Schnellbaecher, Anton Lindig, Maxime Le Mignon, Tim Hofmann, Brit Pardon, Stephanie Bellmaine, Aline Zimmer
Summary: Long-term storage of cell culture media can lead to browning, primarily due to degradation products of tryptophan. Some identified compounds not only darken the solution but also exhibit toxicity against CHO cells. The addition of the cell-culture-compatible antioxidant α-ketoglutaric acid can effectively stabilize components, prevent degradation, and reduce ammonia production in cell culture media.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Martina Zimmermann, Harald Kolmar, Aline Zimmer
Summary: The study demonstrates that SSC shows good cellular performance in cell culture, but the cellular uptake mechanism remains unclear. Through transporter inhibition and overexpression experiments, it was found that x(c)(-) may facilitate the cellular uptake of SSC.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Veronika Dill, Florian Pfaff, Aline Zimmer, Martin Beer, Michael Eschbaumer
Summary: Animal cell culture is essential for biopharmaceutical production, but the molecular mechanisms involved in cell adaptation are largely unknown. Research has shown differences in receptor expression and actin cytoskeleton organization between adherently growing BHK cells and suspension cells.
Article
Biotechnology & Applied Microbiology
Christine Hilde Weiss, Janine Stephanie Caspari, Corinna Merkel, Aline Zimmer
Summary: Cell culture medium formulations are chemically defined to reduce variability, but raw material impurities can still affect cell performance and protein quality attributes. This study highlights the importance of tightly controlling raw material specifications to ensure consistent cell culture processes.
BIOTECHNOLOGY PROGRESS
(2022)
Article
Virology
Hanna Keck, Bernd Hoffmann, Michael Eschbaumer
Summary: A proficiency test was conducted to verify the reliability of foot-and-mouth disease virus (FMDV) diagnostics provided by regional veterinary laboratories in Germany. The participating laboratories demonstrated proficiency in both nucleic acid detection and antibody detection methods for FMDV, with no false-positive results reported.
Article
Microbiology
James J. Zhu, Carolina Stenfeldt, Elizabeth A. Bishop, Jessica A. Canter, Michael Eschbaumer, Luis L. Rodriguez, Jonathan Arzt
Summary: This study reveals that persistent FMDV infection during the transitional phase is associated with increased Th17 cell activity, changes in chemokine expression, and altered NF kappa B signaling. Additionally, the production of a large amount of AHR ligands in ruminants may explain why FMDV persists in ruminants but not in pigs.
Article
Virology
Hanna Keck, Benedikt Litz, Bernd Hoffmann, Julia Sehl-Ewert, Martin Beer, Michael Eschbaumer
Summary: Safe transport and inactivation of diagnostic samples is crucial for infectious diseases diagnostics. In the case of foot-and-mouth disease virus (FMDV), naked full-length viral RNA can be infectious. However, studies conducted on cattle showed that injection of naked FMDV RNA can lead to infection, while non-invasive exposure to the RNA does not result in infection. Inactivated FMDV samples containing full-length viral RNA pose a negligible risk of infecting animals.
Article
Virology
Hanna Keck, Michael Eschbaumer, Martin Beer, Bernd Hoffmann
Summary: Six different biosample collection cards were compared for their ability to inactivate viruses and stabilize viral nucleic acid. Most cards successfully inactivated RNA viruses, with some challenges in inactivating LSDV. Differences were also observed in nucleic acid stabilization among the cards.
Article
Cell Biology
Alisa Schnellbaecher, Aline Zimmer
Summary: LC-MS/MS was used to investigate the stability and degradation products of thiamin in different liquid feed formulations. The necessity of thiamin in the feed was assessed, and it was found that thiamin can be removed without affecting cell performance and metabolome. This study provides profound insights on thiamin reactivity and necessity, suggesting the removal of the dispensable and instable vitamin for the development of next generation feeds.
Article
Biotechnology & Applied Microbiology
Susanne Bohl, Maxime Le Mignon, Thomas Kilian, Aline Zimmer
Summary: Undesired proteoforms were detected during the manufacture of a therapeutic fusion protein produced in CHO cells. Low molecular weight proteoforms with low glycan site occupancy and sialylation content were identified. The quality of the fusion protein was found to be linked to the sodium chloride content of the medium. A mitigation strategy was developed to increase the yield of highly glycosylated Fc-fusion protein.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)