期刊
ZEBRAFISH
卷 14, 期 2, 页码 161-168出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/zeb.2016.1386
关键词
FISH; rDNAs; repetitive DNAs; Robertsonian rearrangements; TTAGGGn probe
资金
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
- Fundacao Araucaria (Fundacao Araucaria de Apoio ao Desenvolvimento Cientifico e Tecnologico do Estado do Parana)
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
- SETI/UGF (Secretaria de Estado da Ciencia, Tecnologia e Ensino Superior/Unidade Gestora do Fundo do Parana)
Cytogenetic studies in fish of the Rineloricaria genus have already shown a high variation in diploid number (2n). Along with fusion/fission events for 2n alteration, inversions contribute to the diversification of chromosome formulae within this group. The present study assessed different populations/species of the Rineloricaria aiming to describe the karyotype organization of its members and understand the mechanisms that lead to the variation of chromosome numbers. Cytogenetic data showed distinct karyotype organization among Rineloricaria populations/species studied, ranging in diploid number from 46 to 64 chromosomes, syntopic species and two karyomorphs in Rineloricaria pentamaculata. Using ribosomal DNAs (rDNAs) and TTAGGGn probes by fluorescence in situ hybridization in species with low diploid numbers, we detected sites of 18S rDNA, 5S rDNA, and TTAGGGn in centromeric regions of metacentric chromosomes, which participated in chromosome rearrangements like centric fusions. In species with high 2n, centric fissions probably occurred in karyotypic diversification. In this study, we assessed the telomeric instability, chromosomal breaks, and rearrangements due to interstitial telomeric site vestiges detection, in addition to the probable role of rDNAs in chromosome fusions in karyotypic diversification of this group.
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