期刊
YEAST
卷 34, 期 8, 页码 335-342出版社
WILEY
DOI: 10.1002/yea.3236
关键词
Rhodosporidium toruloides; carotenoids; Agrobacterium tumefaciens-mediated transformation; T-DNA insertion mutants
资金
- National Natural Science Foundation of China [31501464]
- China Postdoctoral Science Foundation [2016 M591419]
- Educational Commission of Liaoning Province of China [2016 J018]
The red yeast Rhodosporidium toruloides is a known lipid producer capable of accumulating large amounts of triacylglycerols and carotenoids. However, it remains challenging to study its carotenoid production profiles owing to limited biochemical information and inefficient genetic tools. Here we used an Agrobacterium tumefaciensmediated transformation (ATMT) to change its carotenoid production and profiles. We constructed R. toruloides NP11 mutant libraries with ATMT, selected three mutants with different colours, characterized their carotenoid products by high-pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis and assured differences among those strains in terms of carotenoid production and its composition profiles. We then located T-DNA insertion sites using the genome walking technology and provided discussions in terms of the new phenotypes. This study is the first of its kind to change the carotenoid production profiles in R. toruloides. Copyright (C) 2017 John Wiley & Sons, Ltd.
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