Article
Engineering, Environmental
Zifang Chi, Shijie Ju, Wenjing Wang, Huai Li, Yi-Hao Luo, Bruce E. Rittmann
Summary: This study demonstrated simultaneous Cr(VI) and NO3- bioreductions driven by C2H6 oxidation in a membrane biofilm reactor (MBfR). The biofilm was mainly composed of cocci and bacillus, and a Cr(III) precipitate was the major product of microbial Cr(VI) reduction.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
Yulu Wang, Chun-Yu Lai, Mengxiong Wu, Yarong Song, Shihu Hu, Zhiguo Yuan, Jianhua Guo
Summary: The study revealed that the oxygen supply rate has a dual role in selenium reduction rates and microbial communities, depending on the presence of dissolved oxygen. An oxygen supply rate of 184 mg/L.d significantly enhanced selenium reduction rates.
Article
Engineering, Environmental
Yuchun Yang, Zhongyi Lu, Mohammad Azari, Boran Kartal, Huan Du, Mingwei Cai, Craig W. Herbold, Xinghua Ding, Martin Denecke, Xiaoyan Li, Meng Li, Ji-Dong Gu
Summary: A new genus of anammox bacteria, Candidatus Loosdrechtia aerotolerans, has been discovered in a full-scale wastewater-treating biofilm system. This genus possesses unique enzymes involved in nitrate reduction and oxidative stress resistance. The discovery expands our understanding of the ecophysiology of anammox bacteria and provides valuable insights for anammox-based wastewater treatment systems operation.
Article
Engineering, Chemical
Jing Ma, Wei Wei, Guotong Qin, Lei Jiang, Ngie Hing Wong, Jaka Sunarso, Shaomin Liu
Summary: This study presents an efficient reactor design that integrates an electrocatalytic packed-bed reactor with a membrane for nitrate removal. The reactor system shows excellent nitrate removal and nitrogen selectivity, highlighting the potential for large-scale nitrate remediation.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Engineering, Environmental
Jing Ma, Wei Wei, Guotong Qin, Tianliang Xiao, Weiqiang Tang, Shuangliang Zhao, Lei Jiang, Shaomin Liu
Summary: This study introduces a porous tubular carbon membrane as the electrode with catalysts for nitrate removal reactions. The achieved nitrate removal rate is significantly higher than other methods, with high conversion and selectivity to nitrogen. The use of Cu-Pd bimetal catalysts inside the membrane enhances activity and selectivity, leading to improved nitrate removal efficiency.
Article
Environmental Sciences
Parsa Mohajeri, Carol M. S. Smith, Henry Wai Chau, Niklas Lehto
Summary: Nitrate contamination is a significant threat to water bodies, but low-cost materials like allophanic soil and carbon-based bio-wastes have shown potential in reducing nutrient concentrations. A new media called ALLODUST was evaluated for enhancing nitrate removal, with promising results in reducing both nitrate concentrations and nitrous oxide emissions. The study demonstrates the efficiency of the new operating approach in wastewater treatment and environmental protection.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Rongfen Chen, Yan Zhou
Summary: This study demonstrated a microbial activity driven oxygen influx across a microporous hollow fibre membrane by tracking changes in volume and gas composition of entrapped air supply. The results showed that active biofilm in membrane aerated biofilm reactor can effectively uptake oxygen, potentially leading to greater energy savings in some applications.
ENVIRONMENTAL RESEARCH
(2021)
Article
Chemistry, Analytical
Huan Lv, Qian Yang, Yiliang Chen, Xiaolong Xu, Changyu Liu, Jianbo Jia
Summary: This study cultivated a biofilm reactor in situ for rapid determination of biochemical oxygen demand in seawater, without the need for phosphate buffer solution to avoid secondary pollution. Optimal effects of temperature and flow rate were observed, showing a wide linear range and high detection sensitivity under the optimal conditions.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Environmental
Han Zhang, Weijia Gong, Ying Xue, Weichen Zeng, Langming Bai, Guibai Li, Heng Liang, How Yong Ng
Summary: Simulated-sunlight assisted MABR (L-MABR) can enhance the removal efficiency of sulfamethoxazole (SMX) and reduce the production of antibiotic resistance genes (ARGs). Photolysis dominates the SMX removal in L-MABR, with the help of ultraviolet (UV) and reactive oxygen species (ROS) to accelerate the degradation process of SMX.
Article
Engineering, Environmental
Yongsun Jang, Sang-Hoon Lee, Na-Kyung Kim, Chang Hoon Ahn, Bruce E. Rittmann, Hee-Deung Park
Summary: In a hydrogen-based membrane biofilm reactor (H-2-MBfR), the thickness of biofilm is crucial for denitrification. Thick biofilms have lower removal fluxes due to substrate transport resistance. This study operated the H-2-MBfR under different loading rates of oxyanions, and observed improved performance and stable denitrification under stressful conditions. Microbial community analysis showed dominant heterotrophs and the presence of functional genes related to organic carbon metabolism and homoacetogenesis.
Article
Engineering, Environmental
Mengxiong Wu, Chun -Yu Lai, Yulu Wang, Zhiguo Yuan, Jianhua Guo
Summary: Nitrate contamination is a common problem in water environments and poses health risks. This study investigates the feasibility of using propane and butane as electron donors for nitrate reduction. The results showed that propane and butane could continuously remove nitrate under oxygen-limiting conditions, and certain microorganisms were responsible for the oxidation of these gases and the reduction of nitrate.
Article
Engineering, Environmental
Yulu Wang, Chun-Yu Lai, Mengxiong Wu, Xuanyu Lu, Shihu Hu, Zhiguo Yuan, Jianhua Guo
Summary: This study showed that increasing copper concentration significantly enhanced the perchlorate reduction rate in a methane-based membrane biofilm reactor, as well as promoted methane oxidation and perchlorate reduction rates through adjusting the abundance of functional genes. A higher copper concentration also increased microbial community diversity and facilitated the synergistic association between methanotrophs and perchlorate reducers.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Inorganic & Nuclear
Jun Ding, Wenyi Li, Qingqing Chen, Jiafang Liu, Shu Tang, Zhiwei Wang, Longwei Chen, Haimin Zhang
Summary: This work proposes an integrated strategy of non-thermal plasma (NTP) and electrocatalysis technique to realize the indirect harvesting of ammonia (NH3) from air. The strategy activates air to form NOx-, which is further electrochemically reduced to NH3. The self-assembled tandem plasma system, consisting of gliding arc discharge plus microwave discharge with pulse modulation tandem mode (GAD + MD-PM), achieves high NOx- yields. The PtCu alloy with a 3D hexapod structure (PtCu HPs) serves as an effective electrocatalyst for NH3 production.
INORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Chemistry, Inorganic & Nuclear
Jun Ding, Wenyi Li, Qingqing Chen, Jiafang Liu, Shu Tang, Zhiwei Wang, Longwei Chen, Haimin Zhang
Summary: In this study, a strategy was proposed to harvest indirect ammonia from air using the integration of the non-thermal plasma technique and electrocatalysis technique. The self-assembled tandem plasma system was constructed to achieve high NOx- yields, and the plasma-treated KOH solution was used as the electrolyte for the electrochemical reduction reaction. PtCu alloy with a 3D hexapod structure was designed as an effective electrocatalyst, exhibiting remarkable NH3 yield rate and faradaic efficiency. This work provides a potential strategy for sustainable nitrogen fixation and NH3 production.
INORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Environmental Sciences
Jiaqi Wang, Mingyue He, Yufen Li, Fang Ma, Ping Zheng, Baolan Hu
Summary: This study successfully achieved rapid enrichment of denitrifying methanotrophs using a series hollow-fiber membrane biofilm reactor for 90 days. The results showed that the series reactor effectively counteracted the inhibitory effect of dissolved oxygen on denitrifying methanotrophic archaea, resulting in a high growth rate.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Rui Sun, Ying Xu, Yonghong Wu, Jun Tang, Sofia Esquivel-Elizondo, Philip G. Kerr, Philip L. Staddon, Junzhuo Liu
Summary: The study showed that periphytic biofilm can maintain nutrient accumulation capacity under temperature fluctuations by increasing biodiversity, with the adaptation mechanisms of benthic microbial communities helping to sustain this functionality. The findings also emphasize the important role of periphytic biofilm in influencing nutrient cycling in aquatic ecosystems under temperature changes.
ENVIRONMENTAL TECHNOLOGY
(2021)
Article
Engineering, Environmental
Junzhuo Liu, Huijie Zhang, Liying Yan, Philip G. Kerr, Songhe Zhang, Yonghong Wu
Summary: The study found that intact nanoparticle-Ag and Ag+ both have negative impacts on the photosynthesis of benthic algae, but through different pathways. Ag+ is more likely to damage the oxygen-evolving complex and thylakoid membrane, while intact nanoparticle-Ag has less impact. In response to the stress of AgNPs, benthic algae increase light energy absorption to maintain electron transport efficiency and adjust the expression of certain proteins to adapt to the environment.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Diana C. Calvo, Aura Ontiveros-Valencia, Rosa Krajmalnik-Brown, Cesar Torres, Bruce E. Rittmann
Summary: Microbiological conversion of CO2 into biofuels and/or organic industrial feedstock using autotrophic anaerobic bacteria in membrane biofilm reactor has shown that controlling the ratio of H-2 to IC influences microbial chain elongation process and the types and quantities of products produced. Proper management of IC availability and H-2 supply allows control over community structure and function, affecting the chain length of the carboxylates and alcohols produced.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Diego A. Esquivel-Hernandez, Jonathan S. Garcia-Perez, Xiaoyin Xu, Sanya Metha, Juan Maldonado, Siqing Xia, He-Ping Zhao, Bruce E. Rittmann, Aura Ontiveros-Valencia
Summary: The reduction of selenate in hydrogen-fed membrane biofilm reactors was studied in combination with other common electron acceptors. Results showed that the microbial communities had a high diversity and resilience in terms of selenate and nitrate reductions, influenced by the rare biosphere. Changes in electron acceptor or donor loading altered the phylogenetic structure of the communities, but biofilm communities were resilient.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Materials Science, Paper & Wood
Crisel A. Mejia-Rivas, Ana M. Bailon-Salas, Luis A. De la Pena-Arellano, Maria D. J. Rodriguez-Rosales, Luis A. Ordaz-Diaz
Summary: The study shows that using Opuntia ficus-indica mucilage as a coadjuvant can effectively improve the COD removal rate in the treatment of mezcal vinasses through the coagulation-flocculation process. However, the addition of ferric chloride with OFI mucilage did not show satisfactory results in the removal process.
Article
Engineering, Environmental
Junzhuo Liu, Yanmin Zhou, Pengfei Sun, Yonghong Wu, Jan Dolfing
Summary: The study revealed a significant positive correlation between nitrogen immobilized in biofilm biomass and soil organic carbon (SOC) content. High SOC, especially elevated levels of dissolved organic carbon (DOC), were found to trigger increased bacterial and algal diversity, biomass accumulation, and inorganic nitrogen assimilation in phototrophic biofilms. Metatranscriptomics results showed distinct expression of key proteins related to photosynthesis and nitrogen immobilization at high SOC and DOC levels.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Materials Science, Paper & Wood
Ana M. Bailon-Salas, Luis A. Ordaz-Diaz, Pedro A. Dominguez-Calleros
Summary: The study found that Agave durangensis mezcal vinasse has fungicide activity against forest phytopathogenic fungi. The inhibition effect on the fungi was better with lower pH and sterile raw vinasse.
Article
Environmental Sciences
Junzhuo Liu, Haiying Lu, Lirong Wu, Philip G. Kerr, Yonghong Wu
Summary: This study revealed the interactions between DOM and periphytic biofilms and their effects on changes in bioactive phosphorus fractions, providing practical information for designing periphytic biofilm based biofertilizers from the perspective of soil DOM.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Engineering, Chemical
Alexis Ruben Bracamontes-Ruelas, Luis Alberto Ordaz-Diaz, Ana Maria Bailon-Salas, Julio Cesar Rios-Saucedo, Yolanda Reyes-Vidal, Liliana Reynoso-Cuevas
Summary: This article provides an overview of emerging pollutants in wastewater and the environment, discussing their entry pathways and distribution. It also presents advanced oxidation processes and biological treatment methods for removing these pollutants.
Article
Microbiology
Pengfei Sun, Yin Chen, Junzhuo Liu, Ying Xu, Lei Zhou, Yonghong Wu
Summary: Based on a national-scale field investigation in China, the nitrogen accumulation potential of periphytic biofilms decreases with increasing latitude and longitude. The nitrogen cycling pathways suggest that soil nitrogen and N-2 are two potential sources for nitrogen accumulation, while some of the accumulated nitrogen may be lost through emissions. Periphytic biofilms are beneficial for enhancing nitrogen content in paddy fields, but they also accelerate greenhouse gas emissions.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Junzhuo Liu, Lirong Wu, Lina Gong, Yonghong Wu, Andrew J. Tanentzap
Summary: Phototrophic biofilms play a crucial role in shaping the molecular composition and accumulation of dissolved organic matter (DOM) in periodically flooding-drying soils. They increase the abundance of labile DOM compounds and richness of molecular formulae during growth, while decreasing the relative abundance of labile components during decomposition. These findings provide insights into the potential use of phototrophic biofilms for enhancing DOM bioactivity and soil fertility in agricultural settings.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Jose de Jesus Montoya-Rosales, Aura Ontiveros-Valencia, Diego A. Esquivel-Hernandez, Claudia Etchebehere, Lourdes B. Celis, Elias Razo-Flores
Summary: Microbial communities in dark fermentation continuous systems are influenced by substrate type, concentration, and product accumulation. This study used metatranscriptomics and quantitative PCR to investigate the effect of high organic loading rates on microbial community diversity and expression of key dark fermentative genes. The results showed that the microbial communities consisted of H-2-producing bacteria, homoacetogens, and lactic acid bacteria. Increasing the organic loading rate influenced the abundance of genes and gene transcripts related to H-2 production and homoacetogenesis. Adding silicone oil increased H-2 production and the abundance of related genes and gene transcripts.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Environmental Sciences
Bumkyu Kim, Jacob Novitch, Aura Ontiveros-Valencia, Marcela Vega, Robert Nerenberg
Summary: This study evaluated the effectiveness of a liquid-permeable membrane biofilm reactor in supplying performance-enhancing chemicals to the base of a biofilm. The results showed that adding chlorate to a denitrifying biofilm increased the abundance of specialized bacteria and maintained high denitrification efficiency. In a nitrifying biofilm, adding a protozoan inhibitor resulted in increased biofilm thickness and higher removal efficiency of ammonium.
Article
Ecology
F. de Jesus Rodriguez-Flores, Cynthia M. Nunez-Nunez, Luis A. Ordaz-Diaz, Carlos Alvarez-Alvarez, Angelica Rivera Montoya
Summary: The goal of the study was to investigate the growth and adaptability of Californian red worms in diesel-contaminated soil, as well as the efficiency of hydrocarbon degradation in vermicomposting. Results showed that the vermicomposting process with Californian red worms can achieve up to 97% diesel removal efficiency, making it an effective alternative for bioremediation of hydrocarbon-contaminated soils.
Review
Materials Science, Paper & Wood
Ana M. Bailon-Salas, Luis A. Ordaz-Diaz, Pablito M. Lopez-Serrano, Monica Y. Flores-Villegas, Pedro A. Dominguez-Calleros
Summary: Pests have negative impacts on the economy and the environment, necessitating effective pest control measures. Compounds found in plants and lignocellulosic materials derivatives can act against pests, potentially serving as insecticides. Further research on richness, biodiversity, functionality, and microbials is crucial.
Article
Engineering, Environmental
Rong-Gui Zhu, Chang-Gui Pan, Feng-Jiao Peng, Chao-Yang Zhou, Jun-Jie Hu, Kefu Yu
Summary: This comprehensive survey investigated the occurrence, bioaccumulation, and trophic magnification of parabens and their metabolite 4-HB in a marine food web. Results showed that parabens were the predominant pollutants in marine organisms, with significant bioaccumulation from sediments. The estimated trophic magnification factor indicated biomagnification for MeP and trophic dilution for 4-HB. Overall, the risks for humans consuming marine organisms were found to be low.
Article
Engineering, Environmental
Andres F. Torres-Franco, Deborah Leroy-Freitas, Cristina Martinez-Fraile, Elisa Rodriguez, Pedro A. Garcia-Encina, Raul Munoz
Summary: Anaerobic and microalgae-based technologies have emerged as sustainable alternatives for municipal wastewater treatment. However, the presence of viruses in the treated wastewater is a major concern for reuse applications. This study assessed the ability of these technologies to reduce viruses during secondary wastewater treatment. The results showed that all technologies were effective in reducing the concentration of viruses, with microalgae-based treatment exhibiting the highest potential for reducing the disinfection requirements of treated wastewater.
Article
Engineering, Environmental
Young Gwang Kim, Sae Yun Kwon, Spencer J. Washburn, Scott C. Brooks, Ji Won Yoon, Lucien Besnard
Summary: The study uses Hg isotope ratios to identify the sources and exposure pathways of mercury in bivalves, finding that dissolved Hg phases in the water column are the primary source and exposure pathway to bivalves. This provides new insights into using bivalves as bioindicators for sediment quality monitoring.
Article
Engineering, Environmental
Hui Geng, Ying Xu, Rui Liu, Dianhai Yang, Xiaohu Dai
Summary: This study investigates the effect of cation exchange resin (CER) on the sequential recovery of hydrogen and methane from anaerobic digestion (AD) and the corresponding mechanisms. The results show that CER can simultaneously enhance the production of hydrogen and methane by promoting the solubilisation, hydrolysis, and acidification of organic matter. Additionally, CER facilitates effective contact between bacteria and organic particulates and reduces the energy barrier for mass transfer during methane production. The study also reveals changes in the microbial community structure and metagenomics during the AD process.
Article
Engineering, Environmental
Xiaojing Lin, Zhan Jin, Shunfeng Jiang, Zhiquan Wang, Suqing Wu, Ke Bei, Min Zhao, Xiangyong Zheng
Summary: Dehumidification combined with addition of absorbent resin supplement (ARS) was used to achieve rapid evaporation of non-pretreated urine, resulting in high water evaporation efficiency and nutrient recovery.
Article
Engineering, Environmental
Yangli Che, Chaoran Lin, Shen Li, Jiao Liu, Longhai Zhu, Shilei Yu, Nan Wang, Haoshuai Li, Mutai Bao, Yang Zhou, Tonghao Si, Rui Bao
Summary: Hydrodynamic processes play a crucial role in the transmission of sediments, microbial assembly, and organic carbon redistribution in the ocean. Through experiments and analysis, we found that hydrodynamics shape the assembly of microbial communities and control the redistribution of different sourced organic carbon, thereby influencing microbial-mediated biogeochemical transformation.
Article
Engineering, Environmental
Chao Chen, Yu Yang, Nigel J. D. Graham, Zhenyu Li, Xingtao Yang, Zhining Wang, Nadia Farhat, Johannes S. Vrouwenvelder, Li -an Hou
Summary: The fouling of seawater reverse osmosis membranes is a persistent challenge in desalination. This study monitored the operational performance of a desalination plant for 7 years and the fouling development in different areas of membrane modules. The findings showed that operational performance declined over time and fouling mainly occurred at the feed side of the modules, with the highest microbial diversity. Keystone species like Chloroflexi and Planctomycetes played an important role in maintaining community structure and biofilm maturation. Polysaccharides, soluble microbial products, marine humic acid-like substances, and inorganic substances contributed to fouling. Overall, biofouling had a significant impact on membrane fouling after 7 years of operation.
Article
Engineering, Environmental
Dan Li, Jieyi Sun, Yibo Fu, Wentao Hong, Heli Wang, Qian Yang, Junhong Wu, Sen Yang, Jianhui Xu, Yunfei Zhang, Yirong Deng, Yin Zhong, Ping'an Peng
Summary: Sulfidation-oxidation treatment of magnetite (Fe3O4) enhances the production of dark center dot OH, which can efficiently degrade dissolved organic matter (DOM) and accelerate carbon cycling.
Article
Engineering, Environmental
Cheng Yu, Kaijun Wang, Kaiyuan Zhang, Ruiyang Liu, Pingping Zheng
Summary: This study implemented a microaerobic-aerobic configuration in a full-scale municipal wastewater treatment facility and investigated the effects on sludge characteristics, pollutant removal, microbial community, and granulation mechanisms. The results showed successful transition from flocculent-activated sludge to well-defined AGS after two months of operation. The primary pathways for pollutant removal were simultaneous nitrification, denitrification, and phosphorus removal. Moreover, the incorporation of internal separators induced shifts in the flow pattern, which promoted granulation.
Article
Engineering, Environmental
Zhe Zhang, Shaoyang Hu, Guangrong Sun, Wei Wang
Summary: Halogenated aromatic disinfection byproducts (DBPs), such as halogenated phenols, have garnered widespread attention due to their high toxicity and prevalence. This study reports on the analysis, occurrence, and cytotoxicity of a group of emerging halogenated aromatic DBPs, known as halogenated polyhydroxyphenols (HPPs), in drinking water.
Article
Engineering, Environmental
Shengyue Chen, Jinliang Huang, Peng Wang, Xi Tang, Zhenyu Zhang
Summary: Accurate prediction of river water quality is crucial for sustainable water management. This study introduces wavelet analysis and transfer learning techniques to assist LSTM modeling, proposing a newly coupled modeling approach that improves short-term prediction of river water quality.
Article
Engineering, Environmental
Bang Du, Xinmin Zhan, Piet N. L. Lens, Yifeng Zhang, Guangxue Wu
Summary: Efficient anaerobic digestion relies on the cooperation of different microorganisms with different metabolic pathways. This study investigated the effects of different operational modes and the addition of powdered activated carbon (PAC) on ethanol metabolic pathways. The results showed that the SBR mode and the presence of CO2 facilitated ethanol metabolism towards propionate production, while the CFR mode with extended solids retention time enriched Geobacter. Adjusting operational modes and PAC addition can modulate anaerobic ethanol metabolism and enrich Geobacter.
Article
Engineering, Environmental
Wanfa Wang, Si-Liang Li, Jun Zhong, Yuanbi Yi, Fujun Yue, Zenglei Han, Qixin Wu, Ding He, Cong-Qiang Liu
Summary: This study compares the carbon biogeochemical processes in karst and non-karst regions within large thermal stratified river-reservoir systems. The results demonstrate that karst reservoirs have a reduced potential for carbon emissions and highlight the importance of considering geologic settings to improve accuracy in regional and global CO2 emission estimates.
Article
Engineering, Environmental
Chunxia Jiang, Zelong Zhao, Dong Zhu, Xiong Pan, Yuyi Yang
Summary: This study analyzed the occurrence and distribution of antibiotic resistance genes (ARGs) in different environmental media of the Yangtze River using metagenomics. Core resistome dominated by multidrug resistance genes was found in all samples, while rare resistome dominated by various resistance genes was more prevalent in plasmids. Specific bacteria were identified as hosts for both core and rare resistomes, with high clinical concern ARGs found in the rare resistome. Particle-associated environment provided the most ideal conditions for resistome hosts. This study provided insights into the genetic locations of ARGs and the community assembly mechanisms of ARG hosts in freshwater environments.
Article
Engineering, Environmental
Yu Zhang, Yongtao He, Linchun Jia, Lei Xu, Zheng Wang, Yueling He, Ling Xiong, Xumeng Lin, Hong Chen, Gang Xue
Summary: By synergizing organic carbon source, thiosulfate, and zero-valent iron, efficient mixotrophic denitrification of oligotrophic secondary effluent can be achieved. Thiosulfate plays a vital role in promoting TN removal efficiency, while corrosion of Fe0 releases OH- to neutralize H+ from thiosulfate-driven denitrification, creating a suitable environment for denitrification. The coordination of thiosulfate and Fe0 maintains the dominance of Thiobacillus for denitrification.