Article
Agricultural Engineering
Huan Li, Teng Cai, Yijing Gao, Qicai Dai, Xinyu Liu, Xue Chen, Xueqin Lu, Guangyin Zhen
Summary: An upflow blanket filter (UBF) reactor with porous fixed cylinder carriers was used to investigate the formation mechanism of anammox granules. The UBF achieved the highest nitrogen removal efficiency of 93.19 +/- 3.39% at a nitrogen loading rate of 3.6 kg-N/m3/d and HRT of 2 h. Tryptophan-like proteins were found to be vital for granule formation, and the analysis of 16s rRNA revealed the important role of SBR1031 in cell aggregation. The anammox granules developed a two-layered spatial structure consisting of an outer-layer of ammonia oxidizing bacteria and an inner-core of anaerobic ammonia oxidizing bacteria. Introduction of porous fixed cylinder carriers effectively prevented biomass loss and flotation.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Bingbing Ma, Xiaojing Zhang, Aihua Gao, Chuang Ma, Yulin Hou, Zirui Zhao, Hao Hu
Summary: The CANON process was utilized for treating composting wastewater, resulting in decreased ammonia levels and increased total nitrogen removal rate. Microorganisms secreted more extracellular polymeric substances to counteract cell damage caused by harmful substances, maintaining system stability.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Environmental Sciences
Yujie Dai, Xiaolan Lin, Yi Luo, Jing Sun, Yun Tian
Summary: This study investigated the responses and relative contributions of ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB), anaerobic ammonium oxidizing (anammox) bacteria, and denitrifying bacteria in mangrove sediments after spiking urea. The results indicated that nitrification (primarily mediated by AOA)-denitrification process played the most important role in nitrogen removal from the amendment of nitrogen short-term input in the mangrove sediments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Ting Lan, Yuxiao Huang, Xi Song, Ouping Deng, Wei Zhou, Ling Luo, Xiaoyan Tang, Jian Zeng, Guangdeng Chen, Xuesong Gao
Summary: Nitrogen management measures such as urease inhibitors, synthetic nitrification inhibitors, and biochar are commonly used in agriculture to mitigate nitrogen loss and increase fertilizer recovery efficiency. In a study conducted on calcareous soil under rice cropping, it was found that a combination of BNIs and UIs had the most effective results in reducing nitrogen loss and improving FRE by utilizing synergistic effects.
ENVIRONMENTAL POLLUTION
(2022)
Article
Engineering, Environmental
Jing Guo, Xingke Wu, Suyu Liu, Yu Zhang, Zhiqiang Cai
Summary: The current research hotspot is the use of biological methods to degrade high ammonia nitrogen wastewater. Three high-efficiency ammonia-oxidizing bacteria were isolated and purified from wastewater for constructing compound bacteria, showing promising results in degradation efficiency in a pilot test.
JOURNAL OF WATER PROCESS ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Umesh Ghimire, Veera Gnaneswar Gude, Renotta Smith, John P. Brooks, Dewayne Deng
Summary: The nitritation-anammox biocathode microbial desalination cell showed high efficiency in nitrogen and salinity removal, with low energy consumption and high net energy output.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Environmental Sciences
Ahmed Elsayed, Jaecheul Yu, Taeho Lee, Younggy Kim
Summary: This study proposed a real-time aeration scheme based on nitrite concentration to control the competition between nitrite oxidizing bacteria (XNOB) and Anammox bacteria (XANA) in single-stage Anammox processes. The simulation results showed that maintaining dissolved oxygen (DO) at around 0.10 mg-O2/L was ideal for effective nitrite creation by ammonia oxidizing bacteria (XAOB) while slowing down the growth of XNOB. Nitrite concentration was found to be the rate-limiting parameter for efficient nitrogen removal in single-stage Anammox processes.
ENVIRONMENTAL RESEARCH
(2022)
Article
Environmental Sciences
Youqi Tao, Rui Shi, Linjing Li, Suhui Xia, Jianyong Ning, Wenlai Xu
Summary: This study addressed the issues of sludge floating and uneven mass transfer in up-flow partial denitrification/anammox (PDA) reactors by implementing two strategies: adding biological carriers and applying mechanical stirring. The results showed that both strategies effectively prevented sludge flotation and deterioration of settling performance. Mechanical agitation had a more significant positive impact on PDA performance compared to the addition of biocarriers as it achieved more adequate mass transfer.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Review
Engineering, Environmental
Yang-Fan Deng, Fei-xiang Zan, Hao Huang, Di Wu, Wen-tao Tang, Guang-Hao Chen
Summary: This review systematically summarizes and discusses the sulfur-based denitrification with anammox (SDA) process, including its biochemistry, biokinetic reactions, reactor performance, and application. The dominant functional bacteria and microbial interactions in the SDA process are also discussed. The paper provides an in-depth insight into the coupling mechanism of SDA and its potential application in biological nitrogen removal.
Article
Biotechnology & Applied Microbiology
Jing Lu, Yiguo Hong, Ying Wei, Ji-Dong Gu, Jiapeng Wu, Yu Wang, Fei Ye, Jih-Gaw Lin
Summary: The study analyzed the rates of ammonia oxidation (AO) and nitrite oxidation (NO) in the activated sludge from Xinfeng WWTPs, revealing strong activity of both processes. The research also identified AOB and Nitrospira as the main catalysts for nitrification in anammox-inoculated WWTPs, leading to efficient nitrogen removal.
Review
Microbiology
Shimin Lu, Xingguo Liu, Chong Liu, Guofeng Cheng, Runfeng Zhou, Yayuan Li
Summary: AOA and AnAOB play a crucial role in the nitrogen removal process of aquaculture pond water, especially in seasonal low temperatures and anoxic sediment layers. Their application in bioreactors for autotrophic nitrogen removal shows potential for reducing greenhouse gas emissions and promoting environmentally sustainable pond aquaculture.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Adrian Bozal-Leorri, Guntur Subbarao, Masahiro Kishii, Leyre Urmeneta, Victor Kommerell, Hannes Karwat, Hans-Joachim Braun, Pedro Ma Aparicio-Tejo, Ivan Ortiz-Monasterio, Carmen Gonzalez-Murua, Ma Begona Gonzalez-Moro
Summary: Synthetic nitrification inhibitors (SNI) and biological nitrification inhibitors (BNI) are promising tools to limit agricultural nitrogen pollution. Introducing the chromosome region (Lr#n-SA) controlling BNI production into wheat cultivars can enhance nitrogen uptake and utilization.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biodiversity Conservation
Weifang Hu, Mianrun Chen, Xue Lan, Guoliang Li, Bin Wang, Dong Yao Sun, Xianbiao Lin
Summary: This study found significant shifts in potential ammonia oxidation rates (PARs) and gene abundances of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) along different salinity gradients. The PARs were higher under high salinity conditions, and the AOB gene abundance was greater than AOA under moderate and high salinity. This has important implications for nitrogen cycling in estuarine sediments.
ECOLOGICAL INDICATORS
(2022)
Article
Environmental Sciences
Shishi He, Zhirong Zhao, Zhichao Tian, Chi Xu, Yuan Liu, Da He, Yinghui Zhang, Maosheng Zheng
Summary: This study analyzed activated sludge samples from municipal and industrial wastewater treatment plants and found that comammox bacteria were more abundant than ammonia-oxidizing bacteria and ammonia-oxidizing archaea in municipal plants, while this trend did not hold true for industrial plants. Furthermore, a higher diversity of comammox bacteria was found in municipal samples. This study provides important insights into the abundance and diversity of comammox bacteria in municipal and refinery wastewater treatment systems.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Engineering, Environmental
Huihui Cui, Liang Zhang, Qiong Zhang, Xiyao Li, Yongzhen Peng
Summary: This study found that comammox bacteria, which were previously overlooked, are the most important ammonia-oxidizing microbes in Anammox systems, and play a significant role in nitrogen removal.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
Daniel Valenzuela-Heredia, Camila Panatt, Marisol Belmonte, Oscar Franchi, Dafne Crutchik, Jacques Dumais, Jose Ramon Vazquez-Padin, Yves Lesty, Alba Pedrouso, Angeles Val del Rio, Anuska Mosquera-Corral, Jose Luis Campos
Summary: A two-stage system was used to remove nitrogen from dewatering liquor of municipal WWTP sludge. Two strategies were tested to start up the system, demonstrating that dilution of THP/AD effluents is not required. Experimental results showed that inhibitory effects of organic compounds on the activity of ammonia oxidizing bacteria can be eliminated in the PN reactor.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Alba Roibas-Rozas, Montserrat Nunez, Anuska Mosquera-Corral, Almudena Hospido
Summary: Salinity is changing in aquatic systems due to human activities and climate change, and its effects on overall ecosystems are still not well understood. This research introduces a new framework to study the impacts of salinity variations, including the possible positive effects of emissions. The framework was validated by applying it to an estuarine area in northwestern Spain, where sharp drops in salt concentration have caused mass mortalities of shellfish.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Environmental Sciences
Lucia Argiz, David Correa-Galeote, Angeles Val del Rio, Anuska Mosquera-Corral, Rebeca Gonzalez-Cabaleiro
Summary: It was found that the mode of oxygen, carbon, and nitrogen supply determines the enrichment of the culture in specific populations, thus influencing the type of intracellular compounds preferentially accumulated. Coupled carbon and nitrogen feeding regime mainly selects for TAG producers, while uncoupled feeding leads to PHA or TAG production depending on the rate of carbon supply under specific aeration rates and feast and famine phases lengths.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Bruno Garcia Silva, Patricia Perez-Calleja, Eugenio Foresti, Robert Nerenberg
Summary: The foam-aerated biofilm reactor (FABR) is a novel biofilm process that can simultaneously remove carbon and nitrogen from wastewater. This research explored biofilm development within the foam sheet and determined the impact of advective transport on mass transfer and performance. The study found that a dense nitrifying biofilm formed on the aerated side of the foam, while the rest of the foam sheet had a high porosity foam-biofilm layer.
ENVIRONMENTAL TECHNOLOGY
(2023)
Article
Polymer Science
David Correa-Galeote, Lucia Argiz, Angeles Val del Rio, Anuska Mosquera-Corral, Belen Juarez-Jimenez, Jesus Gonzalez-Lopez, Belen Rodelas
Summary: The biosynthesis of PHAs from industrial wastes using MMCs enriched in PHA-accumulating bacteria is a promising alternative to petroleum-based plastics. This study investigated the shifts in bacterial communities in lab-scale reactors fed with fish-canning effluents, and found that a double growth limitation strategy effectively modulated the relative abundances of PHA-accumulating bacteria and increased PHA accumulation, regardless of saline conditions.
Editorial Material
Green & Sustainable Science & Technology
Jose Luis Campos, Anuska Mosquera-Corral, Angeles Val del Rio, Alba Pedrouso
Article
Biochemistry & Molecular Biology
Santo Fabio Corsino, Gaetano Di Bella, Francesco Traina, Lucia Argiz Montes, Angeles Val del Rio, Anuska Mosquera Corral, Michele Torregrossa, Gaspare Viviani
Summary: In this study, a biological strategy was employed in the MBR system to reduce membrane fouling tendency. By controlling the operating conditions, the organic matter was directed towards the accumulation of intracellular polymers (PHA), leading to a decrease in extracellular polymeric substances (EPS) and a reduction in fouling rate. This highlights the possibility of minimizing membrane fouling and recovering bioplastic precursors from wastewater in line with the circular economy model.
Article
Environmental Sciences
Jessica Costa Lopes, Bruno Garcia Silva, Maria Eduarda Simoes Dias, Rodrigo Braz Carneiro, Marcia Helena Rissato Zamariolli Damianovic, Eugenio Foresti
Summary: The removal of nutrients from sewage is still a challenge in conventional treatment plants. This study explored the nitrogen and phosphorus removal from anaerobic pre-treated sewage using a structured fixed bed reactor. It was found that high removal efficiencies could be achieved under the appropriate operating conditions.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
J. M. S. Oliveira, J. S. Poulsen, E. Foresti, J. L. Nielsen
Summary: A multiple stage anaerobic system was proven effective for reductive decolorization of azo dyes in the presence of sulfate. The system achieved significant bulk color removal and organic matter and sulfate reduction in separate reactors. By utilizing amplicon sequencing and correlation analysis, the microbial communities and pathways involved in azo dye removal were identified, with reductive decolorization being mainly associated with hydrogen-producing pathways and the involvement of an azoreductase from Lactococcus lactis. The findings enhance our understanding of the role of different microbial communities in anaerobic treatment of azo dyes and can aid in the design of improved solutions for textile effluent treatment.
Article
Environmental Sciences
J. M. S. Oliveira, C. A. Sabatini, A. J. Santos-Neto, E. Foresti
Summary: Most studies on the degradation of textile azo dyes focus on color as a parameter, but it is susceptible to interference. This study proposes a method for determining a sulfonated azo dye in wastewater using solid-phase extraction and liquid chromatography - electrospray ionization tandem mass spectrometry. The results showed that the dye undergoes hydrolysis and has structural changes in aqueous solution, but its dyeing capacity remains unchanged. Color remains a reliable parameter for monitoring unstable azo compounds in aqueous solution, but a more robust method is needed for the speciation of the dye's hydrolyzed products.
ENVIRONMENTAL POLLUTION
(2023)
Article
Engineering, Environmental
P. Carrera, L. Strubbe, A. Val del Rio, A. Mosquera-Corral, E. I. P. Volcke
Summary: The effect of salinity on the treatment of fish-canning wastewater using aerobic granular sludge was assessed using a one-dimensional biofilm model. The model incorporated a non-competitive inhibition term to describe the salt inhibition of heterotrophic and nitrifying bacteria. The model was calibrated and validated using experimental lab-scale data, and accurately predicted the removal of COD and nitrogen species under different salinity conditions.
ENVIRONMENTAL SCIENCE-WATER RESEARCH & TECHNOLOGY
(2023)
Article
Engineering, Chemical
Alba Pedrouso, Nicolas Morales, Belen Rodelas, David Correa-Galeote, Angeles Val del Rio, Jose Luis Campos, Jose Vazquez-Padin, Anuska Mosquera-Corral
Summary: In this study, a pilot-scale two-stage partial nitritation (PN) and anammox (AMX) system was installed in a municipal wastewater treatment plant for auto-trophic nitrogen removal. The system operated without temperature control and experienced the same variations in wastewater characteristics as the plant. The developed strategy was simple, chemical-free, and had short start-up periods.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Engineering, Environmental
Cristiano Leal, Daniela P. P. Mesquita, Antonio L. Amard, Eugenio C. C. Ferreira, Angeles Val del Rio
Summary: This study successfully predicted the morphological and structural parameters of sludge in the presence of pharmaceutical active compounds using a sequencing batch reactor with aerobic granular sludge. These results highlight the importance of this method for monitoring dysfunctions in aerobic granular sludge.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Engineering, Environmental
S. Santorio, A. Val del Rio, C. L. Amorim, L. Arregui, P. M. L. Castro, A. Mosquera-Corral
Summary: The study evaluated the performance of a Continuous Flow Granular Reactor (CFGR) for treating freshwater RAS stream during two consecutive dry seasons. The configuration with a mechanical stirrer and sieve showed the best results in terms of biomass retention and nitrogen removal, achieving granulation in 55 days with high removal percentages of ammonium and nitrite.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)