期刊
VIRUS RESEARCH
卷 227, 期 -, 页码 110-114出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2016.09.020
关键词
Tembusu virus; Heat shock protein A9; Receptor
类别
资金
- National Natural Science Foundation of China [31172345]
- China Postdoctoral Science Foundation [2015M571714]
- Jiangsu Planned Projects for Postdoctoral Research Funds [1402025B]
- Innovation of Agricultural Sciences in Jiangsu province [CX(14)2091]
This study attempts to identify receptor elements for Tembusu virus (TMUV) on DF-1 cells. Using coimmunoprecipitation and virus overlay protein binding assays, we identified a TMUV-binding protein of approximately 70-kDa on DF-1 cell membranes. Mass spectroscopy identified the protein to be heat shock protein (HSP) A9, which was reconfirmed by an anti-HSPA9 antibody. Indirect immunofluorescence demonstrated a significant degree of colocalization between HSPA9 and TMUV on cell surface. Additionally, an antibody against HSPA9 could inhibit TMUV infection in DF-1 cells in a dose-dependent manner. These results might suggest that HSPA9 is a putative receptor for TMUV. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据