Article
Medicine, General & Internal
Livia Mara Silva, Lorena Rodrigues Riani, Marcelo Silva Silverio, Olavo dos Santos Pereira-Junior, Frederico Pittella
Summary: This article discusses the use of rapid nucleic acid extraction procedures for reliable diagnosis during the COVID-19 pandemic. By comparing different extraction reagents, transport media, viral load levels, and PCR machines, the study finds that rapid nucleic acid extraction procedures can be applied for reliable diagnosis and reduce the time and risk of contamination during RT-qPCR diagnosis.
Article
Medical Laboratory Technology
Mei Yang, Shuang Cao, Yong Liu, Zhijie Zhang, Rui Zheng, Yuzhong Li, Jie Zhou, Chengguo Zong, Desheng Cao, Xiaosong Qin
Summary: This study evaluated the performance of five commercial RT-qPCR diagnostic kits for SARS-CoV-2 used in China. The results showed that all kits passed the performance verification, except for one. Da An and Liferiver demonstrated excellent clinical diagnostic specificity and sensitivity. This study can guide the selection or optimization of RT-qPCR diagnostic test kits for SARS-CoV-2.
CLINICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Shrawan Kumar, Anjali Kharb, Aman Vazirani, Rajinder Singh Chauhan, Goutam Pramanik, Mrittika Sengupta, Souradyuti Ghosh
Summary: Nucleic acid amplification technique (NAAT)-assisted detection is crucial for pathogen molecular diagnostics, but prior nucleic acid extraction is required. Traditional extraction methods limit the use of qPCR or PCR-like NAAT methods in resource-constrained settings.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Virology
Xin-Xin Shen, Feng-Yu Li, Meng Qin, Guo-Hao Zhang, Meng-Yi Zhang, Hong Liu, Xiu-Li Sun, Zhen-Jiang Xin, Xue-Jun Ma
Summary: GeneClick is a device for nucleic acid self-testing of SARS-CoV-2, with three modules: a sampling kit, a microfluidic chip-based disposable cartridge, and an amplification reader. Clinical evaluation showed that GeneClick had a sensitivity and specificity of 97.93% and 99.72% respectively, outperforming antigen tests.
Article
Biology
Seatthanan Pewlaoo, Siratcha Phanthong, Thida Kong-Ngoen, Sirijan Santajit, Witawat Tunyong, Shutipen Buranasinsup, Kampon Kaeoket, Techit Thavorasak, Pornpan Pumirat, Nitat Sookrung, Wanpen Chaicumpa, Nitaya Indrawattana
Summary: Porcine epidemic diarrhea virus (PEDV) infection is a significant acute diarrheal disease in swine, with severe clinical manifestations and high economic impact. A novel RT-RPA-NALF technique was developed for rapid and specific detection of PEDV, showing promising results in clinical and simulated samples.
Article
Infectious Diseases
Guilherme Augusto Minozzo, Thaila Francini Corona, Elaine Cristina Rodrigues da Cruz, Wagner Antonio Chiba de Castro, Louise Bach Kmetiuk, Andrea Pires Dos Santos, Alexander Welker Biondo, Irina Nastassja Riediger
Summary: Rabies, a lethal zoonotic disease, can be diagnosed using direct immunofluorescence and mouse inoculation test. To replace the mouse inoculation test, the World Health Organization recommends using molecular biology techniques like RT-qPCR. Recently, a real-time PCR protocol called LN34 has been validated to detect all rabies virus variants worldwide.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Chemistry, Analytical
Yujie Liu, Hui Liu, Guanliu Yu, Wenbo Sun, Muhammad Aizaz, Guiwen Yang, Lei Chen
Summary: A highly efficient single-tube detection platform based on RPA and CRISPR reaction system has been developed to rapidly and accurately detect MRSA infection. The platform demonstrates satisfactory selectivity and can achieve detection within 30 minutes. It shows outstanding performance in sensitivity, specificity, and resistance identification.
ANALYTICA CHIMICA ACTA
(2023)
Article
Multidisciplinary Sciences
Menglu Hu, Zhiqiang Qiu, Zirong Bi, Tian Tian, Yongzhong Jiang, Xiaoming Zhou
Summary: This article proposes a CRISPR diagnostic method based on optochemical control, which addresses the issues of contamination risks and insufficient sensitivity in nucleic acid amplification-based diagnostics. The method achieves comparable detection sensitivity and specificity to PCR, and avoids contamination risks through photocontrol.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Food Science & Technology
Chenang Lyu, Feifeng Lu, Zhentao Shi, Dapeng Wang
Summary: The study developed an ISC-RT-qPCR assay for detecting encapsulated RVA in oysters with higher sensitivity and accuracy than RT-qPCR. When applied to oysters collected from four different farms, ISC-RT-qPCR showed a higher detection rate of RVA compared to RT-qPCR, indicating its potential as a better method for disease monitoring and control.
Article
Chemistry, Multidisciplinary
Xander F. van Kooten, Yana Rozevsky, Yulia Marom, Efrat Ben Sadeh, Amit Meller
Summary: Most RNA-based COVID-19 diagnostic methods use enzymatic amplification for high sensitivity but rely on arbitrary thresholding. This study introduces a solid-state nanopore sensing technology for label-free quantification of SARS-CoV-2 RNA in clinical nasal swab samples. The method allows for ratiometric quantification of RNA abundance through single-molecule counting and length-based nanopore identification of dsDNA. The results show that this approach can provide quantitative positive outcomes in clinical samples classified as negative by RT-qPCR.
Article
Chemistry, Analytical
Xiaoyu Zhao, Yuxin Huang, Xiang Li, Weihao Yang, Yaoyi Lv, Wei Sun, Jiajun Huang, Shengli Mi
Summary: Researchers have developed a centrifugal microfluidic chip integrated with chitosan-modified magnetic microspheres for pH-induced nucleic acid extraction. The system demonstrates comparable performance in terms of nucleic acid concentration and purity, detection specificity and stability, and fast detection duration. This development is significant for portable, efficient, and simple nucleic acid diagnosis, especially under resource-limited conditions.
Article
Biochemistry & Molecular Biology
Evelyn Kelemen, Eva Adam, Stella Marta Sagi, Aniko Goblos, Lajos Kemeny, Zsuzsanna Bata-Csorgo, Marta Szell, Judit Danis
Summary: Psoriasis is a chronic inflammatory skin disease influenced by both genetic and environmental factors. Increased levels of free nucleic acids in psoriatic skin are associated with IL-23 expression. The study reveals that free nucleic acids regulate IL-23 mRNA expression in epithelial keratinocytes through TLR3 receptor and specific signaling pathways, contributing to the development of an inflammatory milieu favorable for psoriatic symptoms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Rashid Aman, Tin Marsic, Gundra Sivakrishna Rao, Ahmed Mahas, Zahir Ali, Madain Alsanea, Ahmed Al-Qahtani, Fatimah Alhamlan, Magdy Mahfouz
Summary: This study presents a rapid and sensitive detection platform for mass-scale screening of SARS-CoV-2 in low-resource settings. The platform combines CRISPR/Cas gene editing technology with isothermal amplification methods to enable detection of SARS-CoV-2 at a single temperature in less than an hour. The authors also provide detailed guidelines for developing and establishing this one-pot assay, and the clinical validation shows high sensitivity and specificity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Tin Marsic, Zahir Ali, Muhammad Tehseen, Ahmed Mahas, Samir Hamdan, Magdy Mahfouz
Summary: The fusion of catalytically inactive SpCas9 endonuclease and VirD2 relaxase allowed for sensitive and specific nucleic acid detection in lateral flow assays, providing a rapid and cost-effective platform for detecting SARS-CoV2 without cross-reactivity with other coronaviruses.
Article
Biotechnology & Applied Microbiology
Jian Jiao, Yiqi Liu, Mengli Yang, Jingcheng Zheng, Chonghuai Liu, Wenxiu Ye, Shangwei Song, Tuanhui Bai, Chunhui Song, Miaomiao Wang, Jiangli Shi, Ran Wan, Kunxi Zhang, Pengbo Hao, Jiancan Feng, Xianbo Zheng
Summary: The study found that Mb2Cas12a enzyme has stronger trans-cleavage activity, especially at lower temperatures. By designing a reaction vessel that physically separates different steps while maintaining a closed system, the sensitivity and specificity of the diagnosis can be improved, and contamination can be effectively prevented. By improving the CRISPR genome editing technology, highly sensitive and specific detection of multiple sample types can be achieved.
PLANT BIOTECHNOLOGY JOURNAL
(2023)