期刊
VIROLOGY
卷 502, 期 -, 页码 123-132出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2016.12.021
关键词
FMDV; Adenovirus; O1Manisa; Foot-and-mouth; Disease; Cross-protection; Ad5-FMD; Vaccine; Swine
类别
资金
- USDA CRIS [1940-32000-057-00]
- ARS-USDA
- Directorate of Science and Technology of the Department of Homeland Security [HSHQPM-13X-0013]
- USDA-University of Connecticut [58-1940-4-003]
- PIADC Research Participation Program
A human adenovirus (Ad5) vectored foot-and-mouth disease virus (FMDV) O1-Manisa subunit vaccine (Ad5-O1Man) was engineered to deliver FMDV O1-Manisa capsid and capsid-processing proteins. Swine inoculated with Ad5-O1Man developed an FMDV-specific humoral response as compared to animals inoculated with an empty Ad5-vector. Vaccinated animals were completely protected against homologous challenge at 7 or 21 days post-vaccination. Potency studies exhibited a PD50 of about 10(7) pfu/animal while a dose of 4x 10(7) pfu/animal fully protected swine against FMDV intradermal challenge. In-vitro cross-neutralization analysis distinctly predicted that swine vaccinated with Ad5-O1Man would be protected against challenge with homologous FMDV O1Man Middle East-South Asia (ME-SA) topotype and also against recent outbreak strains of Mya-98 South East Asia (SEA) lineage including O1-UK-2001 and O1-South Korea-2010. These results indicate that recombinant Ad5-O1Man is an effective, safe and cross-reacting vaccine that could potentially be used preventively and in outbreak situations, to control FMDV O Mya-98 lineage in swine.
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