期刊
VIROLOGY
卷 508, 期 -, 页码 45-53出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2017.05.002
关键词
PA-X protein; Swine influenza virus; Virulence; Immune responses
类别
资金
- National Natural Science Foundation of China [31522058, 31572536]
- Beijing Science and Technology New Star program [Z161100004916115]
- Chinese Universities Scientific Fund [2014XJ007]
- Chang Jiang Scholars Program
Previous studies have identified a functional role of PA-X for influenza viruses in mice and avian species; however, its role in swine remains unknown. Toward this, we constructed PA-X deficient virus (Sw-FS) in the background of a Triple-reassortment (TR) H1N2 swine influenza virus (SW) to assess the impact of PA-X in viral virulence in pigs. Expression of PA-X in TR H1N2 SW enhanced viral replication and host protein synthesis shutoff, and inhibited the mRNA levels of type I IFNs and proinflammatory cytokines in porcine cells. A delay of proinflammatory responses was observed in lungs of pigs infected by wild type SW (Sw-WT) compared to Sw-FS. Furthermore, Sw-WT virus replicated and transmitted more efficiently than Sw-FS in pigs. These results highlight the importance of PA-X in the moderation of virulence and immune responses of TR SW in swine, which indicated that PA-X is a pro-virulence factor in TR SW in pigs.
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