期刊
VIROLOGY
卷 512, 期 -, 页码 124-131出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2017.09.016
关键词
Herpes simplex virus; HSV-1; Viral genomic sequences; Latency; Illumina sequencing; Strain origins; Phylogenetics; Reactivation; Neurovirulence; Viral pathogenesis
类别
资金
- National Institutes of Health (NIH) grants from National Center for Advancing Translational Sciences (NCATS) [UL1 TR00038]
- Cancer Center Support Grant [P30CA016087]
- National Institutes of Health [AI103933, AI073898, GM056927, AI103268, N272201400019C]
Herpes simplex virus 1 (HSV-1) is a widespread pathogen that persists for life, replicating in surface tissues and establishing latency in peripheral ganglia. Increasingly, molecular studies of latency use cultured neuron models developed using recombinant viruses such as HSV-1. GFP-US11, a derivative of strain Patton expressing green fluorescent protein (GFP) fused to the viral US11 protein. Visible fluorescence follows viral DNA replication, providing a real time indicator of productive infection and reactivation. Patton was isolated in Houston, Texas, prior to 1973, and distributed to many laboratories. Although used extensively, the genomic structure and phylogenetic relationship to other strains is poorly known. We report that wild type Patton and the GFP-US11 recombinant contain the full complement of HSV-1 genes and differ within the unique regions at only eight nucleotides, changing only two amino acids. Although isolated in North America, Patton is most closely related to Asian viruses, including KOS63.
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