Article
Parasitology
Luciana Doria Ribeiro Cabral, Ana Paula Sato, Renato Silva de Sousa, Ana Paula Rossa, Adrien Wilhelm Dilger Sanches, Caroline Tomasi Bortoleto, Rosangela Locatelli Dittrich
Summary: Limited data is available about the presence of Toxoplasma gondii, Neospora spp., and Sarcocystis neurona in equine placental tissues and amniotic fluid. This study found evidence of Neospora spp. DNA in placentas and amniotic fluid of some mares, suggesting a potential transplacental transmission route for Neospora caninum infection.
VETERINARY PARASITOLOGY
(2022)
Review
Parasitology
Luis Fernando Pita Gondim, Rodrigo Martins Soares, Gaston More, Rogerio Fernando de Jesus, Horwald Alexander Bedoya Llano
Summary: Sarcocystis parasites are obligate heteroxenous cyst-forming coccidia that infect a wide variety of animals, with species shed by South American opossums showing biological and genetic differences from those shed by North American opossums.
REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA
(2021)
Review
Parasitology
J. P. Dubey
Summary: This review provides an update on the history, life cycle, diagnosis, and treatment of three infections in horses: Sarcocystis neurona, Neospora spp., and Toxoplasma gondii.
Article
Microbiology
Tautvile Sukyte, Dalius Butkauskas, Evelina Juozaityte-Ngugu, Saulius Svazas, Petras Prakas
Summary: The study aimed to identify Sarcocystis species in intestinal scrapings of Northern Goshawks and Eurasian Sparrowhawks in Lithuania. Sarcocystis calchasi, a protozoan parasite causing respiratory and neurological diseases in birds, was detected for the first time in Lithuania. Different Sarcocystis species were identified, and the prevalence and species richness were higher in Northern Goshawks compared to Eurasian Sparrowhawks. This study provides important insights into the distribution and diversity of Sarcocystis species in avian hosts.
Article
Parasitology
Laura da Silva Ney, Samira Salim Mello Gallo, Nicole Brand Ederli, Francisco Carlos Rodrigues de Oliveira
Summary: Protozoa of the genus Sarcocystis are obligate heterogenous parasites with both definitive and intermediate hosts. This study aimed to determine the main lesions that can occur in acute and chronic infections in intermediate hosts after ingesting infective sporocysts shed by opossums, using budgerigars as a model. The results showed that acute infection presented characteristic lesions in the liver and lungs, while chronic infection displayed mature cysts mainly in thigh and tongue muscles.
PARASITOLOGY RESEARCH
(2021)
Article
Veterinary Sciences
Benjamin M. Rosenthal
Summary: Apicomplexan species in the genus Sarcocystis form tissue cysts in intermediate hosts, posing a threat to human health when consumed in infected livestock meat, causing intestinal sarcocystosis. Clinical manifestations vary from asymptomatic muscle cysts to severe muscle infections.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Parasitology
Mariele De Santi, Marcos Rogerio Andre, Karin Werther, Luiz Ricardo Goncalves, Rodrigo Martins Soares, Heitor Miraglia Herrera, Filipe Martins Santos, Nayara Yoshie Sano, William Oliveira de Assis, Andreza Castro Rucco, Rosangela Zacarias Machado
Summary: High frequency of Sarcocystis falcatula-like samples and high genetic diversity have been found in South American opossums in Brazil. Sarcocystis spp. isolated from Didelphis albiventris and Didelphis aurita showed ITS-1 and cox1 results consistent with S. falcatula previously described in Brazil.
REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA
(2023)
Article
Parasitology
Ana Paula Sato, Miuriel de Aquino Goulart, Aline Luiza Konell, Marilia de Oliveira Koch, Flavia Moreira da Fonseca, Ana Paula Morel, Rosangela Locatelli-Dittrich
Summary: This study evaluated the serological reactivity of captive raptors serum to T. gondii, N. caninum and S. neurona antigens, with 2.7% reacting to N. caninum, 8.3% to T. gondii, and 11.1% to S. neurona antigens. Factors such as region of sample collection, reason for captivity, and diet were statistically associated with seropositivity to these protozoans. The occurrence of these protozoans in birds of prey highlights the importance of good hygiene and feeding management in reducing the risk of infection.
PARASITOLOGY INTERNATIONAL
(2021)
Article
Immunology
Monireh Khordadmehr, Alireza Sazmand, Pardis Almasi, Parisa Shahbazi, Vahidreza Ranjbar, Domenico Otranto, Andrew Hemphill
Summary: This study provides an overview of the prevalence and exposure to tissue cyst-forming apicomplexan parasites in Iranian domestic rock pigeons through molecular, serological, and histopathological examination. The results demonstrate that pigeons in Iran are infected, particularly with T. gondii, and suggest the need for control strategies to minimize the risk of infection in both pigeons and humans.
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2023)
Article
Parasitology
Hang Zeng, Inge Van Damme, Teresia Wanjiru Kabi, Barbara Soba, Sarah Gabriel
Summary: This study investigated the occurrence of Sarcocystis spp. in cattle sampled from a Belgian slaughterhouse and found that female dairy cattle had the highest Sarcocystis occurrence rate.
PARASITES & VECTORS
(2021)
Article
Biochemistry & Molecular Biology
Luisa Berna, Pablo Marquez, Andres Cabrera, Gonzalo Greif, Maria E. Francia, Carlos Robello
Summary: The study reveals errors in the assembly of the Neospora caninum genome and significant chromosomal rearrangements between Neospora caninum and Toxoplasma gondii, leading to a reduction in their karyotype. Additionally, more than 500 new genes were identified through reannotation of the Neospora caninum genome.
Article
Veterinary Sciences
Guili Yu, Wei Liang, Qiankun Yang, Jinxin Wang, Yu Wang, Tianmeng Zhang, Xiao Zhang, Hui Fan, Panpan Zhao, Lili Cao, Jingquan Dong
Summary: Neospora caninum's dense granule proteins, including GRA4, exhibit potential as vaccine candidates, triggering protective immune responses and significantly improving survival rates and reducing parasite burden.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Parasitology
Yesica Lopez, Alvaro A. Faccini-Martinez, Sebastian Munoz-Leal, Veronica Contreras, Alfonso Calderon, Ricardo Rivero, Marina Munoz, Juan David Ramirez, Salim Mattar
Summary: This study provides the first molecular evidence of Borrelia puertoricensis in Colombia, specifically in opossums, and the first detection of this spirochete in a vertebrate host since its isolation from Ornithodoros puertoricensis in Panama. The detection is also significant due to the epidemiological importance of opossums as reservoirs of zoonotic diseases to humans.
PARASITES & VECTORS
(2023)
Review
Chemistry, Multidisciplinary
Haixiang Yu, Obtin Alkhamis, Juan Canoura, Yingzhu Liu, Yi Xiao
Summary: This review discusses the challenges and advances in isolating and characterizing small-molecule-binding DNA aptamers, including in vitro methodologies for isolation, strategies for optimal binding properties, techniques for characterizing aptamer-target binding and structure, and various small-molecule sensing platforms and detection applications based on aptamers.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemical Research Methods
Laura Guillardin, John J. MacKay
Summary: This study compared two DNA isolation methods and found that the MicroGEM method was advantageous in reducing plastic waste and improving time efficiency. The results demonstrate that this method allows for sufficient and reliable DNA for common analyses.
Article
Microbiology
Laila A. R. Beserra, Carla M. Barbosa, Mikael Berg, Paulo E. Brandao, Rodrigo M. Soares, Fabio Gregori
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2020)
Correction
Microbiology
Laila A. R. Beserra, Carla M. Barbosa, Mikael Berg, Paulo E. Brandao, Rodrigo M. Soares, Fabio Gregori
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Parasitology
Daniela P. Chiebao, Paul M. Bartley, Francesca Chianini, Lauren E. Black, Alison Burrells, Hilda F. J. Pena, Rodrigo M. Soares, Elisabeth A. Innes, Frank Katzer
Summary: This study found that different genotypes of Toxoplasma gondii can induce different immune responses and parasite dynamics, with non-archetypal strains demonstrating higher virulence.
Article
Agriculture, Dairy & Animal Science
Igor C. L. Acosta, Solange M. Gennari, Horwald A. B. Llano, Sebastian Munoz-Leal, Rodrigo M. Soares
Summary: Sarcocystidae is a family of apicomplexan protozoa highly prevalent in vertebrates, with definitive hosts eliminating oocysts or sporocysts that infect intermediate hosts and develop into mature tissue cysts. In 2017, DNA of a potentially new species of Sarcocystis was identified in Chilean skuas, indicating the need for further studies to understand the epidemiology and impact of the infection. Further investigations revealed that the genetic makeup of the parasite detected in skuas was closely related to species using birds as definitive hosts.
Article
Veterinary Sciences
Waleria Borges-Silva, Mariana M. Rezende-Gondim, Gideao S. Galvao, Daniele S. Rocha, George R. Albuquerque, Luis P. Gondim
Summary: The study identified parasites resembling Neospora caninum or Toxoplasma gondii in the cerebrospinal fluid of a dog with neurologic disease, which had not been previously reported in dogs. The detection of T. gondii by CSF cytology was crucial for diagnosing toxoplasmosis in the examined dog.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2021)
Article
Ecology
Aline Diniz Cabral, Chunlei Su, Rodrigo Martins Soares, Solange Maria Gennari, Marcia Aparecida Speranca, Adriana Ruckert da Rosa, Hilda Fatima Jesus Pena
Summary: Studies in Sao Paulo state, Brazil, investigated the molecular frequency and diversity of Sarcocystidae parasites in bats, revealing a variety of these organisms in different bat species. The findings suggest that bats may play a role in the transmission of these parasites and highlight the need to consider Toxoplasma gondii as a differential diagnosis for rabies and other neurological syndromes in bats.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE
(2021)
Article
Parasitology
Horwald A. B. Llano, Rodrigo Martins Soares, Leidy Y. Acevedo-Gutierrez, Juan David Rodas, Gina Polo, Waleria Borges-Silva, Rogerio F. Jesus, Luis F. P. Gondim
Summary: The study found that equids in the North and Northwest regions of Colombia are exposed to S. neurona, but antibodies to Neospora spp. are rare. Risk factors for exposure include poor body condition, older age, and inhabiting climatic conditions associated with tropical very dry forests. Further research is needed to explore the presence of these parasites in other areas and understand the impact of these underdiagnosed coccidia on equids.
Article
Fisheries
Glaucia Pereira Sousa, Rodrigo Martins Soares, Joao Carlos Gomes Borges, Ana Paula Domingos Brito, Daniella Carvalho Ribeiro Oliveira, Thalita Faita, Fernanda Loffler Niemeyer Attademo, Fabia de Oliveira Luna, Radan Elvis Matias Oliveira, Carlos Ibere Alves Freitas, Jociery Einhardt Vergara-Parente, Lara Borges Keid
Summary: The study in northeast Brazil expanded the investigation of Brucella infection in marine mammals, showing all manatee samples tested negative for antibodies and DNA related to Brucella spp. However, Brucella spp. DNA was detected in the kidney of one Guiana dolphin, without successful isolation of the bacteria through microbiological culturing.
JOURNAL OF AQUATIC ANIMAL HEALTH
(2021)
Article
Ecology
Horwald A. B. Llano, Heloise Zavatieri Polato, Lara Borges Keid, Tricia Maria Ferreira de Souza Oliveira, Triciana Zwarg, Alice S. de Oliveira, Thais C. Sanches, Adriana M. Joppert, Luis F. P. Gondim, Rodrigo Martins Soares
Summary: This study represents the first extensive survey of Sarcocystidae species in wild birds in Brazil. It provides molecular evidence of natural infection of S. falcatula in 14 species, including in the order Piciformes, and demonstrates high genetic diversity of S. falcatula in intermediate hosts in South America. Additionally, this study presents evidence of at least three non-described species of Sarcocystis.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE
(2022)
Article
Infectious Diseases
Joao Augusto Franco Leonel, Bruna Tannihao, Julia Assis Arantes, Geovanna Vioti, Julia Cristina Benassi, Roberta Ariboni Brandi, Helena Lage Ferreira, Lara Borges Keid, Rodrigo Martins Soares, Tricia Maria Ferreira de Sousa Oliveira
Summary: Visceral leishmaniasis is a tropical disease caused by the Leishmania infantum parasite, which can infect horses and donkeys, with DNA of L. infantum being found in these animals in Sao Paulo State, Brazil, suggesting potential parasite infection in horses and donkeys from both VL and non-VL endemic areas.
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO
(2021)
Article
Parasitology
Anaia da Paixao Seva, Ana Perola Drulla Brandao, Silvia Neri Godoy, Rodrigo Martins Soares, Helio Langoni, Bruna Cristine Rodrigues, Mariana Zanchetta E Gava, Paula Ferraz de Camargo Zanotto, Tatiana Jimenez-Villegas, Roberto Hiramoto, Fernando Ferreira
Summary: Visceral leishmaniasis (VL) is a significant zoonosis in Brazil, with the identification of parasitized dogs being crucial for disease prevention. However, the accuracy of serological tests such as DPP (R)+ELISA has been questioned. A study in a non-endemic area of Sao Paulo state showed fluctuating seroprevalence rates of VL in dogs over three years.
REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL
(2021)
Review
Parasitology
Luis Fernando Pita Gondim, Rodrigo Martins Soares, Gaston More, Rogerio Fernando de Jesus, Horwald Alexander Bedoya Llano
Summary: Sarcocystis parasites are obligate heteroxenous cyst-forming coccidia that infect a wide variety of animals, with species shed by South American opossums showing biological and genetic differences from those shed by North American opossums.
REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA
(2021)
Article
Agronomy
Diogo Tiago da Silva, Maria Luana Alves, Julio Cesar Pereira Spada, Joao Augusto Franco Leonel, Julia Cristina Benassi, Wilma Aparecida Starke-Buzetti, Helena Lage Ferreira, Lara Borges Keid, Rodrigo Martins Soares, Tricia Maria Ferreira de Sousa Oliveira
Summary: The study found that L. infantum can be present in the reproductive organs of dogs without causing lesions, indicating a potential role of these organs in the transmission of canine leishmaniasis.
Article
Parasitology
Mahyumi Fujimori, Arleana do Bom Parto Ferreira de Almeida, Stella Maria Barrouin-Melo, Luiz Ricardo Paes de Barros Cortez, Malcolm Scott Duthie, Roberto Mitsuyoshi Hiramoto, Flaviane Alves de Pinho, Steven Gregory Reed, Valeria Regia Franco Sousa, Nazare Fonseca Souza, Rodrigo Martins Soares, Jose Eduardo Tolezano, Maria Carmen Arroyo Sanchez, Hiro Goto
Summary: The study aimed to establish an effective serological assay for diagnosing L. infantum infected dogs. The sensitivity of the rK39-, rK28- and rKR95-based ELISA was around 97% with a specificity of 100%. The new diagnostic method showed better performance compared to assays currently in use in Brazil and worldwide.
MEMORIAS DO INSTITUTO OSWALDO CRUZ
(2021)