Article
Allergy
Lisa Hung, Alper Celik, Xiaojun Yin, Kai Yu, Alireza Berenjy, Akash Kothari, Helena Obernolte, Julia E. M. Upton, Katrine Lindholm Bogh, Gino R. Somers, Iram Siddiqui, Martin Grealish, Fayez A. Quereshy, Katherina Sewald, Priscilla P. L. Chiu, Thomas Eiwegger
Summary: This study presents a novel ex vivo model using precision cut intestinal slices to study acute, allergen-specific, IgE-mediated smooth muscle contractions. The model can be used to investigate food allergies and the effects of drugs on allergic responses in the gut.
Article
Microbiology
Gaurav Rawal, Wannarat Yim-im, Ethan Aljets, Patrick G. Halbur, Jianqiang Zhang, Tanja Opriessnig
Summary: Porcine respiratory coronavirus (PRCV) is a mutant strain distinct from transmissible gastroenteritis virus (TGEV). Little is known about recent PRCV isolates, and this study aimed to obtain a contemporary PRCV isolate from US pigs for genetic characterization.
Article
Virology
Chengfan Jiang, Liang Li, Mei Xue, Liyuan Zhao, Xiang Liu, Wenzhe Wang, Li Feng, Pinghuang Liu
Summary: Porcine respiratory CoV (PRCoV), a natural mutant of TGEV, shares pathogenetic similarities with human respiratory CoVs. The lack of an in vitro model for the study of PRCoV infection is a major obstacle. This study successfully developed long-term porcine airway organoids (AOs) that can serve as a promising disease model for PRCoV infection.
JOURNAL OF VIROLOGY
(2022)
Article
Veterinary Sciences
Lei Qin, Fandan Meng, Haijuan He, Yong-Bo Yang, Gang Wang, Yan-Dong Tang, Mingxia Sun, Wenlong Zhang, Xuehui Cai, Shujie Wang
Summary: Trueperella pyogenes is a pathogenic bacterium that causes diseases in various animals. In this study, a multidrug-resistant strain named 20121 was identified and shown to be pathogenic in mouse models and porcine precision-cut lung slices (PCLS). Additionally, dexamethasone was found to relieve bronchoconstriction caused by T. pyogenes 20121 in PCLS.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Jae Hun Kim, Niccole Schaible, Joseph K. Hall, Erzsebet Bartolak-Suki, Yuqing Deng, Jacob Herrmann, Adam Sonnenberg, Holger P. Behrsing, Kenneth R. Lutchen, Ramaswamy Krishnan, Bela Suki
Summary: By measuring tissue stiffness at both the tissue and alveolar scales, we found that the lung tissue of patients with emphysema was 50% softer due to microscopic septal wall remodeling and structural deterioration. Protein expression profiling revealed a wide range of enzymes involved in septal wall remodeling, along with mechanical forces, leading to rupture and structural deterioration of the emphysematous lung parenchyma.
Article
Pharmacology & Pharmacy
Ondrej Martinec, Carin Biel, Inge A. M. de Graaf, Martin Huliciak, Koert P. de Jong, Frantisek Staud, Filip Cecka, Peter Olinga, Ivan Vokral, Lukas Cerveny
Summary: This study utilized human precision-cut intestinal slices (hPCISs) to investigate the induction of functional expression of ABCB1 by rifampicin. Results demonstrated that rifampicin significantly increased gene expression, protein levels, and efflux activity of ABCB1 in hPCISs, suggesting the potential value of hPCISs in evaluating drugs' ABCB1-inducing potency in the human intestine.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Quanhui Yan, Keke Wu, Weijun Zeng, Shu Yu, Yuwan Li, Yawei Sun, Xiaodi Liu, Yang Ruan, Juncong Huang, Hongxing Ding, Lin Yi, Mingqiu Zhao, Jinding Chen, Shuangqi Fan
Summary: This study analyzed the genetic diversity and spatiotemporal distribution characteristics of transmissible gastroenteritis virus (TGEV) and porcine deltacoronavirus (PDCoV) through genome sequence analysis. It identified different genotypes and lineages within these two swine enteric coronaviruses. Positive selection sites were also identified, indicating the importance of spike protein in membrane fusion and adaptive evolution. The study provides new insights into the evolutionary characteristics and transmission diversity of these SeCoVs, emphasizing the need for enhanced surveillance and biosecurity measures in the context of the COVID-19 epidemic.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Changlin Wang, Mei Xue, Peng Wu, Honglei Wang, Zhongqing Liu, Guangzheng Wu, Pinghuang Liu, Keliang Wang, Wanhai Xu, Li Feng
Summary: This study found that Transmissible gastroenteritis virus (TGEV) infection inhibits the expression of anti-coronavirus microRNA through the IRE1 pathway, providing insights into how the virus regulates the host cell response to infection.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Microbiology
Dong-Mei Gao, Hai-Yang Yu, Wei Zhou, Bing-Bing Xia, Hong-Zhang Li, Ming-Li Wang, Jun Zhao
Summary: The research found that the dose of rPoIFN-alpha affects pigs' resistance to TGEV, with a higher dose (2 x 10(7)IU/pig) providing full protection, a moderate dose (2 x 10(6)IU/pig) offering partial protection, and a low dose (2 x 10(5)IU/pig) unable to adapt to the challenge. However, rPoIFN-alpha can delay the onset of clinical symptoms in pigs.
VETERINARY MICROBIOLOGY
(2021)
Article
Immunology
Lingdan Yin, Xiang Liu, Dongmei Hu, Yi Luo, Guozhong Zhang, Pinghuang Liu
Summary: Swine enteric coronaviruses (SECoVs), including PEDV, TGEV, and PDCoV, are the main pathogens causing lethal watery diarrhea in neonatal pigs. Different types of SECoVs induce different transcriptional profiles and immune responses in host epithelial cells, which helps us gain a better understanding of host-SECoVs interactions.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Engineering, Biomedical
Rachel Blomberg, Kayla Sompel, Caroline Hauer, Alex J. Smith, Brisa Pena, Jennifer Driscoll, Patrick S. Hume, Daniel T. Merrick, Meredith A. Tennis, Chelsea M. Magin
Summary: Lung cancer, the leading cause of cancer-related deaths worldwide, still occurs in people who have quit smoking. Researchers have developed an in vitro model of lung cancer premalignancy using precision-cut lung slices embedded in hydrogel and exposed to a cigarette smoke carcinogen. This model provides a foundation for studying the earliest stages of lung carcinogenesis and potential chemoprevention strategies.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Veterinary Sciences
Hongjin Zhou, Kaichuang Shi, Feng Long, Kang Zhao, Shuping Feng, Yanwen Yin, Chenyong Xiong, Sujie Qu, Wenjun Lu, Zongqiang Li
Summary: In this study, a quadruplex real-time quantitative PCR (qRT-PCR) assay was developed for the differential detection of porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV). The assay showed extreme specificity, high sensitivity, and excellent reproducibility. Evaluation of 3236 clinical fecal samples from Guangxi province, China, showed positive rates of 18.26% for PEDV, 0.46% for TGEV, 13.16% for PDCoV, and 0.15% for SADS-CoV. The developed assay provides an accurate method for the differential detection of these four porcine enteric coronaviruses.
VETERINARY SCIENCES
(2022)
Article
Veterinary Sciences
Pei-Hua Wang, Amina Nawal Bahoussi, Pir Tariq Shah, Yan-Yan Guo, Changxin Wu, Li Xing
Summary: Transmissible gastroenteritis virus (TGEV) is a porcine coronavirus that remains elusive despite years of research efforts. A systematic analysis of TGEV genomes showed two distinct evolutionary clades, GI and GII, with Chinese strains clustering with traditional or attenuated vaccine strains within clade GI and recent US strains falling into clade GII. There were significant differences in genomic similarity between Chinese and US strains, and several potential genomic recombination events were identified. Genomic recombination appears to contribute to the expansion of TGEV genomic diversity.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Multidisciplinary Sciences
Eric Simon, Maciej Motyka, Grietje H. Prins, Mei Li, Werner Rust, Stefan Kauschke, Coralie Viollet, Peter Olinga, Anouk Oldenburger
Summary: There is a need for predictive ex vivo models for NAFLD. This study profiles a new human and mouse PCLSs based assay for steatosis in NAFLD using RNASeq. Steatosis is induced by incremental supplementation of sugars, insulin, and fatty acids, and the gene expression regulation is analyzed across different nutrient conditions and time for both human and mouse liver organ derived PCLSs.
Article
Physiology
M. J. R. Ruigrok, M. A. P. Roest, H. W. Frijlink, P. Olinga, W. L. J. Hinrichs, B. N. Melgert
Summary: Chronic obstructive pulmonary disease (COPD) is the third leading cause of death globally, characterized by impaired epithelial repair. The study shows that RANKL protein contributes to lung repair by reducing cell death.
FRONTIERS IN PHYSIOLOGY
(2022)