期刊
VETERINARY MICROBIOLOGY
卷 203, 期 -, 页码 202-210出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2017.02.021
关键词
Thymol; Actinobacillus pleuropneumoniae; Bactericidal; Biofilm; Pneumonia
资金
- National Key Research and Development Program of China [2016YFD0500708]
- National Natural Science Foundation of China [31672559, 31372447]
- Henan Provincial Department of Education [17A230003]
- China Postdoctoral Science Foundation [2016M600578]
- Henan Institute of Science and Technology [2016ZD04]
Actinobacillus pleuropneumoniae is the causative agent of the highly contagious and deadly respiratory infection porcine pleuropneumonia, resulting in serious losses to the pig industry worldwide. Alternative to antibiotics are urgently needed due to the serious increase in antimicrobial resistance. Thymol is a monoterpene phenol and efficiently kills a variety of bacteria. This study found that thymol has strong bactericidal effects on the A. pleuropneumoniae 5b serotype strain, an epidemic strain in China. Sterilization occurred rapidly, and the minimum inhibitory concentration (MIC) is 31.25 mu g/mL; the A. pleuropneumoniae density was reduced 1000 times within 10 min following treatment with 1 MIC. Transmission electron microscopy (TEM) analysis revealed that thymol could rapidly disrupt the cell walls and cell membranes of A. pleuropneumoniae, causing leakage of cell contents and cell death. In addition, treatment with thymol at 0.5 MIC significantly reduced the biofilm formation of A. pleuropneumoniae. Quantitative RT-PCR results indicated that thymol treatment significantly increased the expression of the virulence genes purC, tbpB1 and clpP and down-regulated ApxI, ApxII and Apa1 expression in A. pleuropneumoniae. Therapeutic analysis of a murine model showed that thymol (20 mg/kg) protected mice from a lethal dose of A. pleuropneumoniae, attenuated lung pathological lesions. This study is the first to report the use of thymol to treat A. pleuropneumoniae infection, establishing a foundation for the development of new antimicrobials. (C) 2017 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据