Article
Immunology
Dominic Dolbec, Melanie Lehoux, Masatoshi Okura, Daisuke Takamatsu, Marcelo Gottschalk, Mariela Segura
Summary: In this study, we compared the protective properties of different serotypes of Streptococcus suis using serotype-switched mutants in a mouse infection model. The structure of the capsular polysaccharide (CPS) influenced bacterial survival, antibody binding, and antibody-mediated bacterial killing. CPS of serotypes 3, 4, and 14 allowed more antibody binding and bacterial elimination compared to serotypes 2, 7, and 9. These findings suggest that the varying CPS structures of S. suis provide different levels of protection by affecting antigen availability and elimination by the host immune system.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Genglin Guo, Zhuohao Wang, Quan Li, Yanfei Yu, Yubao Li, Zhongming Tan, Wei Zhang
Summary: This study analyzed the genomic characteristics of Streptococcus parasuis and compared it with Streptococcus suis. It identified some unique features of S. parasuis and provided insights into its interspecific and intraspecific genetic characteristics. These findings are important for further research on serotyping, diagnostics, vaccine development, and the pathogenesis mechanism of S. parasuis.
Article
Infectious Diseases
Shuji Gao, Yamin Shen, Shuo Yuan, Yingying Quan, Xingping Li, Yuxin Wang, Li Yi, Yang Wang
Summary: This study investigated the effects and mechanisms of methyl anthranilate (MA) on Streptococcus suis (S. suis) biofilm. The results showed that MA destroyed the structure of S. suis biofilm, hindered biofilm formation, and reduced the synthesis of capsular polysaccharides, leading to weakened adhesion and antiphagocytosis ability. MA competitively bound to S-adenosylhomocysteine nucleosidase, interfering with AI-2 quorum sensing of S. suis. In a mouse model, MA effectively reduced bacterial burden and inflammatory infiltrates.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Microbiology
Meng Nie, Yuhao Dong, Qing Cao, Dan Zhao, Shuting Ji, Hao Huang, Mingguo Jiang, Guangjin Liu, Yongjie Liu
Summary: In this study, we demonstrate that crRNA inhibits the transcription of the cps operon by repressing the activity of promoter PcpsA, leading to increases in the abilities of adhesion, invasion, and biofilm formation in S. agalactiae. This study highlights the regulatory role of crRNA in bacterial physiology and provides a new explanation for the mechanism of crRNA-mediated endogenous gene regulation in S. agalactiae.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Allison N. Dammann, Anna B. Chamby, Francisco J. Gonzalez, Molly E. Sharp, Karina Flores, Ifrah Shahi, Sophia Dongas, Thomas A. Hooven, Adam J. Ratner
Summary: This study found that specific capsule types may provide an advantage in GBS vaginal colonization, but the success of certain GBS lineages may involve both capsule and non-capsule genetic elements.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Chemistry, Analytical
Raine Garrido, Barbara Baro, Jean Pierre Soubal, Darielys Santana
Summary: This study presents an improved quantitative P-31-NMR methodology for the quantification of C-polysaccharide in capsular polysaccharide preparations, allowing discrimination between phosphocholine residues linked in different positions. The method was successfully applied to samples of vaccine serotypes, showing good quantification performance with a signal/noise ratio of 16:1. Repeatability and intermediate precision evaluation demonstrated the method's stability, while also providing information on the structural identity of phosphate contained in capsular polysaccharides and C-polysaccharide species.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Multidisciplinary Sciences
Rei Nakamoto, Jeric Mun Chung Kwan, Jasmine Fei Li Chin, Hui Ting Ong, Josue Flores-Kim, Caroline Midonet, Michael S. VanNieuwenhze, Xue Li Guan, Lok-To Sham
Summary: Many pathogenic bacteria rely on a layer of capsular polysaccharide (CPS) for virulence, with the bacterial tyrosine kinase (BY-kinase) playing a crucial role in regulating capsule synthesis. The interaction between BY-kinase CpsCD and other proteins influences CPS polymer length and peptidoglycan synthesis in Streptococcus pneumoniae. Autophosphorylation of BY-kinases fine-tunes the activity of CpsC, providing spatiotemporal coordination between CPS and PG synthesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Chaoxiong Yue, Chenlu Hu, Peng Xiang, Siming Zhang, Hongde Xiao, Wei Zhou, Hui Jin, Deshi Shi, Jinquan Li, Lang Xu, Yushan Chen, Yan Zeng
Summary: Streptococcus suis (S. suis) is a significant swine and human pathogen that can cause severe meningitis with high mortality. This study demonstrates that autophagy serves as a cellular defense mechanism in microglial cells following S. suis infection, aiding in limiting bacterial invasion and microglial inflammation.
VETERINARY MICROBIOLOGY
(2021)
Article
Immunology
Quanming Xu, Hong Chen, Wen Sun, Yongyi Zhang, Dewen Zhu, Kul Raj Rai, Ji-Long Chen, Ye Chen
Summary: sRNA23 is a key regulator in the pathogenicity of S. suis 2, enhancing its virulence by directly binding to proteins involved in adhesion and biofilm formation.
Article
Fisheries
Yanjian Yang, Meng Chen, Zhelin Wu, Defeng Zhang, Huiyan Lin, Xiayi Wei, Biao Han, Zheng Guo, Jianmin Ye
Summary: This study found that the phagocytosis of Streptococcus agalactiae by tilapia macrophages was significantly enhanced in cps-deficient mutant Delta cps and sia-deficient mutant Delta neuA. By dampening the inflammatory response and down-regulating the expression of phagocytic receptors, S. agalactiae could interfere with the phagocytosis of macrophages. These findings provide valuable insights into the defense mechanisms of S. agalactiae against the teleost innate immune system and offer clues for vaccine development.
Article
Biochemistry & Molecular Biology
Michelle M. Kuttel
Summary: Streptococcus suis is a potentially pathogenic bacterium that primarily infects swine and causes severe diseases. The capsule polysaccharide (CPS) is a key factor in its virulence. Serotype 2 is the most prevalent worldwide, and developing vaccines targeting its CPS is a major focus. Cross-protection with related serotypes may be possible. Molecular simulation helps explain immunological data and provides insights for rational design of S. suis vaccines.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Microbiology
Jinghui Zhang, Weijie Ye, Kaifeng Wu, Shengnan Xiao, Yuqiang Zheng, Zhaoche Shu, Yibing Yin, Xuemei Zhang
Summary: Streptococcus pneumoniae can alter its cell surface phenotype in response to the host environment; FabT indirectly regulates capsular polysaccharide, while PsrA is responsible for phase variation; Upregulation of PsrA correlates with more T phase variants, while inactivation of PsrA leads to O variants.
Article
Biochemistry & Molecular Biology
Maria Jose Lopez-Martinez, Andelo Beletic, Josipa Kules, Dina Resetar-Maslov, Ivana Rubic, Vladimir Mrljak, Edgar Garcia Manzanilla, Elena Goyena, Silvia Martinez-Subiela, Jose Joaquin Ceron, Alberto Munoz-Prieto
Summary: Through saliva and serum proteomic analysis, we elucidated specific proteome changes in saliva and serum caused by Streptococcus suis infection-induced meningitis, which may reflect different pathophysiological mechanisms and provide new potential biomarkers for this infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Public, Environmental & Occupational Health
N. M. A. Tarini, N. M. Susilawathi, A. A. R. Sudewi, A. Soejitno, N. N. D. Fatmawati, I. P. B. Mayura, A. A. W. Lestari, G. Suputra, I. K. Subrata, C. I. S. D. Astiti, I. N. K. Besung, G. N. Mahardika
Summary: This study investigated a suspected cluster of Streptococcus suis meningitis cases in Sibang Kaja Village, Bali, Indonesia, and identified the source of high-risk food and the presence of the pathogen in pigs. The study found that prompt early treatment of patients and the implementation of a comprehensive One Health approach are crucial in controlling the disease.
Article
Medicine, General & Internal
Javier Diez de los Rios, Esteban Reynaga, Merce Garcia-Gonzalez, Jordi Camara, Carmen Ardanuy, Jordi Cuquet, Maria D. Quesada, Marian Navarro, Anna Vilamala, Noemi Parraga-Nino, Sara Quero, Alba Romero, Rosa M. Benitez, Jacint Altimiras, Maria Luisa Pedro-Botet
Summary: The study focused on S. suis infections in Catalonia over a 9-year period, revealing a small number of cases predominantly affecting middle-aged males, with meningitis being the most common manifestation. More than half of the cases occurred in the last two years and were mainly seen in pig farm workers. While none of the patients died within 30 days, some did experience long-term complications such as hearing loss. Further surveillance is recommended to better understand the prevalence of S. suis infections in the region.
FRONTIERS IN MEDICINE
(2021)
Article
Immunology
Jiao Dai, Liying Lai, Huanyu Tang, Weixue Wang, Shuoyue Wang, Chengping Lu, Huochun Yao, Hongjie Fan, Zongfu Wu
Article
Infectious Diseases
Jinhu Huang, Xi Liu, Hao Chen, Li Chen, Xueping Gao, Zihao Pan, Jian Wang, Chengping Lu, Huochun Yao, Liping Wang, Zongfu Wu
TRANSBOUNDARY AND EMERGING DISEASES
(2019)
Article
Microbiology
Zihao Pan, Jin Liu, Yue Zhang, Shenshen Chen, Jiale Ma, Wenyang Dong, Zongfu Wu, Huochun Yao
VETERINARY MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
David Lalaouna, Jessica Baude, Zongfu Wu, Arnaud Tomasinil, Johana Chicher, Stefano Marzil, Francois Vandenesch, Pascale Romby, Isabelle Caldelari, Karen Moreau
NUCLEIC ACIDS RESEARCH
(2019)
Article
Microbiology
Jiale Ma, Ze Zhang, Zihao Pan, Qiankun Bai, Xiaojun Zhong, Yinchu Zhu, Yue Zhang, Zongfu Wu, Guangjin Liu, Huochun Yao
Article
Immunology
Guangjin Liu, Tingting Gao, Xiaojun Zhong, Jiale Ma, Yumin Zhang, Shiyu Zhang, Zongfu Wu, Zihao Pan, Yinchu Zhu, Huochun Yao, Yongjie Liu, Chengping Lu
INFECTION AND IMMUNITY
(2020)
Article
Microbiology
Yinchu Zhu, Wenyang Dong, Jiale Ma, Yue Zhang, Xiaojun Zhong, Zihao Pan, Guangjin Liu, Zongfu Wu, Huochun Yao
Summary: The study revealed the relatedness between different subpopulations of S. suis serotypes (SS2, SS3, SS7) and capsule switching in CC28 and CC29. Clade 2 SS2 strains showed high genetic similarity with SS3 and SS7, sharing common competence and defensive elements.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Microbiology
Xiaoming Wang, Junjie Sun, Chen Bian, Jianping Wang, Zijing Liang, Yanling Shen, Huochun Yao, Jinhu Huang, Liping Wang, Han Zheng, Zongfu Wu
Summary: The zoonotic pathogen Streptococcus suis SS31 exhibits high heterogeneity, with 75 strains clustered into 3 lineages showing different evolutionary paths and antimicrobial resistance profiles. Additionally, some strains isolated from clinically healthy pigs also showed pathogenicity, highlighting the unique characteristics of SS31 and contributing to public health surveillance and understanding the diversity of S. suis.
VETERINARY MICROBIOLOGY
(2021)
Article
Infectious Diseases
Shuoyue Wang, Miaohang Ma, Zijing Liang, Xinchi Zhu, Huochun Yao, Liping Wang, Zongfu Wu
Summary: This study provides valuable information about the pathogenetic characteristics and antimicrobial resistance of Streptococcus pasteurianus. The capsule was identified as an essential virulence factor for this pathogen, and a mouse infection model was established.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Infectious Diseases
Zhaoying Liu, Qiuhua Xu, Pujun Liang, Zeren Peng, Huochun Yao, Han Zheng, Zongfu Wu
Summary: Streptococcus suis, a severe zoonotic and foodborne pathogen, has distinct population structure and pathogenicity based on various serotypes. This study focused on serotype 8 (SS8) and found that SS8 population exhibited geographical clustering and included strains with global outbreak potential. Several SS8 strains showed virulence in mice and zebrafish, but no specific virulence indicators were identified. All SS8 isolates were classified as multidrug-resistant, with a high prevalence of resistance genes for macrolides, lincosamides, and tetracyclines. These findings highlight the importance of SS8 surveillance.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Immunology
Zijing Liang, Huizhen Wu, Chen Bian, Hao Chen, Yanling Shen, Xueping Gao, Jiale Ma, Huochun Yao, Liping Wang, Zongfu Wu
Summary: This study revealed the mechanism of stable persistence of Streptococcus suis in pig tonsil niche and its competitive survival strategy. It was found that S. suis strain WUSS351 is virulent and multidrug-resistant, and possesses several antimicrobial systems. Additionally, the study demonstrated the T7SS-mediated antagonism of S. suis against other strains. The research also revealed that Lcn351 mainly targets S. suis in pig tonsils. These findings have important implications for the epidemiology and disease progression of S. suis.
Article
Microbiology
Miaohang Ma, Shuoyue Wang, Xinchi Zhu, Xinchun Li, Yinli Bao, Xiang Chen, Zongfu Wu
Summary: Streptococcus pasteurianus is a zoonotic pathogen causing meningitis and bacteremia. This study established a multiplex PCR assay for the detection of S. pasteurianus and found two non-virulent strains with multidrug resistance characteristics. These findings provide important insights into the epidemiology and pathogenesis of this zoonotic bacterium.
Article
Immunology
Xinchi Zhu, Shuoyue Wang, Yu Du, Zijing Liang, Huochun Yao, Xiang Chen, Zongfu Wu
Summary: This study utilized RNA-Seq to reveal the transcriptional landscape of Streptococcus suis under oxidative stress. Several novel genes responsible for resisting oxidative stress were identified, along with a novel aquaporin, Aagp, which plays a crucial role during hydrogen peroxide stress. Additionally, Aagp may be involved in glycerol transport and contributes to S. suis virulence.
Article
Immunology
Pujun Liang, Mingliu Wang, Marcelo Gottschalk, Ana Vela, April A. Estrada, Jianping Wang, Pengcheng Du, Ming Luo, Han Zheng, Zongfu Wu
Summary: This study confirmed the non-negligible pathogenicity of Streptococcus suis serotype 7 and deepened the understanding of its population structure, providing valuable information for improved surveillance of this serotype.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Immunology
Qiankun Bai, Jiale Ma, Ze Zhang, Xiaojun Zhong, Zihao Pan, Yinchu Zhu, Yue Zhang, Zongfu Wu, Guangjin Liu, Huochun Yao