Article
Biotechnology & Applied Microbiology
Henok Ayalew Tegegne, Ivana Kolackova, Martina Florianova, Pierre Wattiau, Tereza Gelbicova, Cecile Boland, Jean-Yves Madec, Marisa Haenni, Renata Karpiskova
Summary: This study revealed the genetic diversity and population structure of Staphylococcus aureus presenting the same spa type, t899, but belonging to different STs. The findings suggest that MLST and spa typing methods should be combined to enhance the accuracy in identifying S. aureus lineages. Additional markers, such as tet(M), sak, and seg genes, could be implemented easily and inexpensively to improve lineage identification.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Judit Szarvas, Mette Damkjaer Bartels, Henrik Westh, Ole Lund
Summary: The study compared clustering analysis results of MRSA and E. faecium using commercial software and open-source software, finding highly concordant phylogenies inferred by the two different technologies. The open-source software PAPABAC has the advantage of generating stable, referable clusters without the need for sequence assembly, and serves as a free-of-charge, open-source alternative to commercial software.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Mostafa Y. Abdel-Glil, Alexandra Chiaverini, Giuliano Garofolo, Antonio Fasanella, Antonio Parisi, Dag Harmsen, Keith A. Jolley, Mandy C. Elschner, Herbert Tomaso, Jorg Linde, Domenico Galante
Summary: A novel cgMLST scheme for Bacillus anthracis has been developed, providing high-resolution strain genotyping. The scheme has been evaluated on 584 genomes from 50 countries and confirmed the genetic distances and clades of B. anthracis. It can be used for rapid and harmonized interlaboratory comparisons, essential for global surveillance and outbreak analysis.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Infectious Diseases
M. D. Bartels, P. Worning, L. P. Andersen, M. Bes, H. Enger, C. G. As, T. A. Hansen, B. J. Holzknecht, K. W. Larssen, F. Laurent, B. Makitalo, B. Pichon, O. Svartstrom, H. Westh
Summary: The research identified a successful emerging clone of MRSA, t304/ST6, which has spread in several European countries and is commonly described in the Middle East. Most isolates were PVL negative and carried SCCmec IVa, with a similar plasmid found in 78% of all isolates.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Microbiology
Bar Zilberman, Yair Motro, Orli Sagi, David Kornspan, Shalom Ben-Shimol, Michael Gdalevich, Yael Yagel, Nadav Davidovitch, Boris Khalfin, Peter Rabinowitz, Lior Nesher, Itamar Grotto, Svetlana Bardenstein, Jacob Moran-Gilad
Summary: This study used whole-genome sequencing to analyze the genomic epidemiology of Brucella melitensis in Southern Israel, revealing a complex transmission pattern of the disease among Bedouin communities. The study found no dominant strain and identified local and household-based transmission as the main modes of spread.
Article
Immunology
Helena M. B. Seth-Smith, Michael Biggel, Tim Roloff, Vladimira Hinic, Thomas Bodmer, Martin Risch, Carlo Casanova, Andreas Widmer, Rami Sommerstein, Jonas Marschall, Sarah Tschudin-Sutter, Adrian Egli
Summary: Clostridioides difficile infection can lead to nosocomial outbreaks, with WGS offering higher resolution epidemiological data compared to traditional PCR-ribotyping, although limitations exist in predicting ribotypes for certain strains.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Hongzhi Zhang, Liang Tian, Taiyao Chen, Wenjie Chen, Yilin Ge, Jing Bi, Zhixin Fang, Min Chen
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) isolates in Chinese hospitals show geographic variation in their prevalence and molecular characteristics. ST764-t002-II has become the predominant lineage, replacing ST5-t002-II, and poses a potential risk of transmission.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shaojie Huang, Yi Li, Chengji Hong, Yafang Jin, Shengkai Li, Xuelian Xu, Yanmei Xia, Leyi Zhang, Yongliang Lou, Wanchun Guan
Summary: The study aimed to investigate the AMR genes, virulence factors, and genetic diversity of Yersinia strains isolated from meats and fish in Wenzhou in 2020 using WGS. The results showed the presence of different Yersinia species, with most strains isolated from beef. Multiple AMR genes and virulence factors were identified, and considerable diversity was observed among the strains. The study provides valuable data for the development of effective antipathogen control strategies.
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2023)
Article
Public, Environmental & Occupational Health
Maryam Dadar, Hanka Brangsch, Saeed Alamian, Heinrich Neubauer, Gamal Wareth
Summary: Brucellosis is a common zoonotic disease in the Middle East, causing economic losses to the livestock industry and posing a great public health concern. This study analyzed the genetic diversity and distribution of Brucella spp. in Iran using whole genome sequencing technology. The results revealed a close relationship between Iranian strains and strains from neighboring and Middle East countries, emphasizing the importance of brucellosis control measures in Iran.
Article
Microbiology
Henrike Krueger-Haker, Xing Ji, Dennis Hanke, Stefan Fiedler, Andrea T. Fessler, Nansong Jiang, Heike Kaspar, Yang Wang, Congming Wu, Stefan Schwarz
Summary: This study investigated the genomic diversity and antimicrobial resistance traits of Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) clonal complex 398 isolates collected from diseased swine in Germany. The isolates displayed close phylogenetic relationships but a wide molecular variety, and harbored a wide range of antimicrobial resistance properties. The study highlights the importance of monitoring and preventing the spread of LA-MRSA in swine husbandry facilities and the human community.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Mette Damkjaer Bartels, Mona Katrine Alberthe Holm, Peder Worning, Bodil Stavad, Uffe Vest Schneider, Heidi Meiniche, Henrik Thiesen, Henrik Westh
Summary: Whole genome sequencing (WGS) has greatly improved the detection of methicillin-resistant Staphylococcus aureus (MRSA) transmission between people. Using WGS and core genome MLST (cgMLST), we describe the transmission of two unique MRSA clones among homeless people in Copenhagen. The study confirms the effectiveness of WGS and cgMLST in revealing MRSA outbreaks, and the use of ETHOS categorization to identify the primary source of spread in the homeless community.
Article
Microbiology
Hauke Toennies, Karola Prior, Dag Harmsen, Alexander Mellmann
Summary: A novel cgMLST scheme for P. aeruginosa was established using a Bayesian approach to determine different groups, incorporating 15 reference strains and 3867 target genes. The scheme was highly reproducible and was validated through comparisons with SNP results and other cgMLST schemes, demonstrating its accuracy in outbreak investigations.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Maria M. M. Konstantinovski, Leo M. M. Schouls, Sandra Witteveen, Eric C. J. Claas, Margriet E. E. Kraakman, Jayant Kalpoe, Eva Mattson, David J. J. Hetem, Erika P. M. van Elzakker, Jos Kerremans, Vishal Hira, Thijs Bosch, Jairo Gooskens
Summary: This study assessed the genetic epidemiology and clinical characteristics of Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in an urban area with a limited livestock population in the Netherlands. The findings suggest that LA-MRSA may cause a significant burden of disease in urban areas, with most infections occurring through inter-human transmission rather than livestock exposure.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Mostafa Y. Abdel-Glil, Uwe Fischer, Dieter Steinhagen, Una McCarthy, Heinrich Neubauer, Lisa D. Sprague
Summary: The study conducted a comparative analysis of Yersinia ruckeri whole genome sequences, revealing their genetic relatedness to other members of the genus and potential genes and mechanisms involved in pathogenicity pathways. It provides new insights into the taxonomy and evolution of Y. ruckeri strains, offering a framework for more efficient control strategies for this pathogen.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jurgita Aksomaitiene, Aleksandr Novoslavskij, Mindaugas Malakauskas
Summary: This study characterized the antimicrobial resistance (AMR) and virulence genes of Campylobacter jejuni isolates from different sources in Lithuania using whole-genome sequencing. The results revealed diverse AMR and virulence gene profiles in C. jejuni, with different patterns of resistance to various antimicrobials. Additionally, the study identified different sequence types and clonal complexes based on core-genome MLST analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Linda Falgenhauer, Can Imirzalioglu, Hiren Ghosh, Konrad Gwozdzinski, Judith Schmiedel, Katrin Gentil, Rolf Bauerfeind, Peter Kaempfer, Harald Seifert, Geovana Brenner Michael, Stefan Schwarz, Yvonne Pfeifer, Guido Werner, Michael Pietsch, Uwe Roesler, Beatriz Guerra, Jennie Fischer, Hannah Sharp, Annemarie Kaesbohrer, Alexander Goesmann, Katja Hille, Lothar Kreienbrock, Trinad Chakraborty
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2016)
Article
Veterinary Sciences
Johanna Hering, Cornelia Froemke, Christiane von Muenchhausen, Maria Hartmann, Bettina Schneider, Anika Friese, Uwe Roesler, Lothar Kreienbrock, Katja Hille
PREVENTIVE VETERINARY MEDICINE
(2016)
Article
Veterinary Sciences
Katja Hille, Inga Ruddat, Annette Schmid, Johanna Hering, Maria Hartmann, Christiane von Muenchhausen, Bettina Schneider, Ute Messelhaeusser, Anika Friese, Rolf Mansfeld, Annemarie Kaesbohrer, Stefan Hoermansdorfer, Uwe Roesler, Lothar Kreienbrock
PREVENTIVE VETERINARY MEDICINE
(2017)
Article
Multidisciplinary Sciences
Denise Rabold, Werner Espelage, Muna Abu Sine, Tim Eckmanns, Alexander Schneebereg, Heinrich Neubauer, Nadine Moebius, Katja Hille, Lothar H. Wieler, Christian Seyboldt, Antina Luebke-Becker
Article
Public, Environmental & Occupational Health
Katja Hille, Nadine Moebius, Manas K. Akmatov, Jutta Verspohl, Denise Rabold, Maria Hartmann, Kathrin Guenther, Nadia Obi, Lothar Kreienbrock
BUNDESGESUNDHEITSBLATT-GESUNDHEITSFORSCHUNG-GESUNDHEITSSCHUTZ
(2014)
Article
Public, Environmental & Occupational Health
Claudia Sievers, Manas K. Akmatov, Lothar Kreienbrock, Katja Hille, Wolfgang Ahrens, Kathrin Guenther, Dieter Flesch-Janys, Nadia Obi, Karin B. Michels, Julia Fricke, Karin H. Greiser, Rudolf Kaaks, Hans-Hartmut Peter, Frank Pessler, Alexandra Nieters, Gerard Krause
BUNDESGESUNDHEITSBLATT-GESUNDHEITSFORSCHUNG-GESUNDHEITSSCHUTZ
(2014)
Article
Public, Environmental & Occupational Health
N. Moebius, K. Hille, J. Verspohl, P. Wefstaedt, L. Kreienbrock
EPIDEMIOLOGY AND INFECTION
(2013)
Article
Microbiology
Lars Valentin, Hannah Sharp, Katja Hille, Uwe Seibt, Jennie Fischer, Yvonne Pfeifer, Geovana Brenner Michael, Silke Nickel, Judith Schmiedel, Linda Falgenhauer, Anika Friese, Rolf Bauerfeind, Uwe Roesler, Can Imirzalioglu, Trinad Chakraborty, Reiner Helmuth, Giuseppe Valenza, Guido Werner, Stefan Schwarz, Beatriz Guerra, Bernd Appel, Lothar Kreienbrock, Annemarie Kaebohrer
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2014)
Article
Veterinary Sciences
Johanna Hering, Katja Hille, Cornelia Froemke, Christiane von Muenchhausen, Maria Hartmann, Bettina Schneider, Anika Friese, Uwe Roesler, Roswitha Merle, Lothar Kreienbrock
PREVENTIVE VETERINARY MEDICINE
(2014)
Article
Microbiology
Katja Hille, Mayala Felski, Inga Ruddat, Johanna Woydt, Annette Schmid, Anika Friese, Jennie Fischer, Hannah Sharp, Lars Valentin, Geovana Brenner Michael, Stefan Hoermansdorfer, Ute Messelhaeusser, Uwe Seibt, Walther Honscha, Beatriz Guerra, Stefan Schwarz, Uwe Roesler, Annemarie Kaesbohrer, Lothar Kreienbrock
VETERINARY MICROBIOLOGY
(2018)
Article
Microbiology
Annemarie Kaesbohrer, Karin Bakran-Lebl, Alexandra Irrgang, Jennie Fischer, Peter Kaempf, Arthur Schiffinanne, Christiane Werckenthin, Matthias Busch, Lothar Kreienbrock, Katja Hille
VETERINARY MICROBIOLOGY
(2019)
Article
Veterinary Sciences
Sylvia Mitrenga, Johanna Popp, Andre Becker, Maria Hartmann, Hatice Ertugrul, Dmitrij Sartison, Susanne Deutsch, Diana Meemken, Lothar Kreienbrock, Katja Hille
PREVENTIVE VETERINARY MEDICINE
(2020)
Article
Agriculture, Dairy & Animal Science
Marco Werhahn Beining, Maria Hartmann, Antina Luebke-Becker, Sebastian Guenther, Katharina Schaufler, Katja Hille, Lothar Kreienbrock
Summary: A study identified factors associated with the carriage of extended spectrum beta-lactamase-producing Escherichia coli in dogs, including husbandry conditions, contact with puppies, feeding of raw meat, food residues, food supplements, and antibiotic treatments of dogs or owners. Understanding these factors is crucial for preventing the spread of pathogens.
Article
Veterinary Sciences
Anke Huels, Cornelia Froemke, Katja Ickstadt, Katja Hille, Johanna Hering, Christiane von Muenchhausen, Maria Hartmann, Lothar Kreienbrock
FRONTIERS IN VETERINARY SCIENCE
(2017)