Article
Fisheries
Yunji Xiu, Baoshan Guo, Jingyuan Yi, Huimin Guo, Hetron Mweemba Munang'andu, Cheng Xu, Shun Zhou
Summary: This study used RNA-seq to analyze the transcriptomic profile of kidney tissue in turbot after immunization with an inactivated bivalent vaccine against Aeromonas salmonicida and Edwardsiella tarda. The results revealed that the vaccine induced upregulation and downregulation of immune-related genes, including cytokines, complement system, and chemokines, enhancing the immune response against these pathogens in turbot.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Lin Su, Huimin Guo, Baoshan Guo, Jingyuan Yi, Zongrui Yang, Shun Zhou, Yunji Xiu
Summary: In recent years, combination vaccines have become necessary to prevent fish diseases caused by multiple infections simultaneously. In this study, an inactivated bivalent vaccine was prepared for Aeromonas salmonicida and Edwardsiella tarda. After vaccination, the vaccinated turbot showed a relative percentage survival (RPS) of 77.1% when challenged with A. salmonicida and E. tarda. The study also found that the vaccinated group had upregulated serum antibody titer and lysozyme activity, as well as increased expression levels of genes related to antigen recognition, processing, and presentation.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Jingjing Cao, Kunihiko Futami, Masashi Maita, Teruyuki Nakanishi, Takayuki Katagiri
Summary: This study investigated the immune response and vaccine efficacy in fish vaccinated with different vaccines. The results showed that the allogeneic + FKC group had the best protection effect, achieved through the induction of CMI. The study provides important information for vaccine development.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Song Sun, Ding Lyu, Yulong Hu, Weiji Wang
Summary: This study aimed to determine the genetic parameters for the resistance of turbot to Edwardsiella tarda in order to design breeding protocols. The results showed consistent and high heritability estimates in the sire-dam model, indicating the successful application of selective breeding for resistance to E. tarda in the breeding population.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Cheng-dong Jin, Mo-fei Li
Summary: The study demonstrates that Omp1Et is a surface-located virulence factor of E. tarda, and recombinant Omp1Et can effectively induce immune protection as a subunit vaccine.
Article
Fisheries
Tasok Leya, Rajendran Kooloth Valappil, Gayatri Tripathi, Pani Prasad Kurcheti, Megha Kadam Bedekar
Summary: In this study, the expression of pIgR gene in Labeo rohita in response to a pDNA vaccine was characterized and its correlation with IgM expression in mucosal tissues was analyzed. The results showed a positive correlation between the expression of pIgR and IgM, suggesting a possible role of pIgR as an IgM transporter in L. rohita.
Article
Fisheries
Hongxiang Wang, Ming Guo, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: The study showed that flounder IFN-gamma could act as a promising adjuvant candidate for fish vaccination, significantly enhancing immune responses and protective effects against E. tarda. Co-vaccination with IFN-gamma could boost specific antibody levels and immune-related gene expressions, leading to higher survival rates in vaccinated fish.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Immunology
Xiao-Mi Sun, Asami Yoshida, Fukutarou Toutani, Takahiro Shimizu, Tatsuya Oda, Kiyoshi Osatomi
Summary: The flagellin genes of the high and low virulent strains of Edwardsiella tarda were cloned and expressed in E. coli. Both recombinant flagellins were found to stimulate the production of NO and TNF-alpha in mouse macrophages. These results suggest that the D0 and D1 domains of the flagellins are sufficient for macrophage activation.
MICROBIAL PATHOGENESIS
(2023)
Article
Immunology
Palanisamy Bothammal, Mohan Ganesh, Vellaisamy Vigneshwaran, Kumarasamy Anbarasu, Karuppiah Ponmurugan, Naif Abdullah Al-Dhabi, Kalimuthusamy Natarajaseenivasan
Summary: The study identified immune expressive proteins HflC, HflK, and YhcI which showed a 60% survivability in clearing Edwardsiella from the host. These immunoreactive proteins induced a strong immune response post-infection, leading to significant production of IL-10, IFN-gamma, Th1, and Th2 mediated mRNA expression, thus proving effective in vaccine production for edwardsiellosis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Huijing Cui, Cong Cong, Lili Wang, Xiaoyu Li, Jibin Li, Huan Yang, Shuying Li, Yongping Xu
Summary: This study discovered a lytic phage named PETp9 which can effectively prevent and control Edwardsiella tarda infection in turbot. The best preventive effect was achieved after 7 days of feeding phage with MOI = 10, and the most effective treatment in the phage-injection therapy was when the phage with MOI = 10 was injected intraperitoneally immediately after the challenge. In phage-immersion therapy, the best therapeutic effect was obtained when the infected turbot were immersed in phage-seawater mixture (MOI = 10) for 3 hours. Furthermore, the activities of non-specific immune-related enzymes in the blood of turbot treated with phage gradually reached normal levels.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Yunjeong Choe, Donghee Lee, Minji Seong, Ju Bin Yoon, Jun Hyeok Yang, Jin-Young Yang, Ki Hwan Moon, Ho Young Kang
Summary: The study identified a potential live vaccine candidate against E. piscicida infection in aquaculture, and found that Fap and Fdp proteins are crucial for flagella formation and motility, as well as for stimulating fish immune response.
JOURNAL OF FISH DISEASES
(2022)
Review
Immunology
Rie Chida, Kazuki Iio, Haruki Kaizuka, Yuji Hirai, Yu Ishida, Gaku Yamanaka
Summary: This article reports a case of a 12-year-old boy with symptoms of chronic diarrhea, abdominal pain, and weight loss, who was suspected to have inflammatory bowel disease but was ruled out by endoscopy and tissue biopsy.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2022)
Article
Infectious Diseases
Yuying Fu, Lishan Zhang, Huanhuan Song, Junyan Liao, Li Lin, Wenjia Jiang, Xiaoyun Wu, Guibin Wang
Summary: This study investigates the role of lysine acetylation and succinylation in the antibiotic resistance mechanism of Edwardsiella tarda. The results demonstrate that these post-translational modifications play important roles in bacterial antibiotic resistance and are enriched in various metabolic pathways. The findings provide insights into the mechanism of antibiotic resistance and may aid future research on the pathogenesis of this bacterium.
Article
Fisheries
Liao-tian Peng, De-li Li, Dai-xiao Yang, Bo Peng
Summary: This study profiles the metabolomic change of tilapia upon E. tarda infection and identifies taurine as a key metabolite that enhances fish immune response against bacterial infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Muhammad Nafees Ur Rehman, Yu Wang, Jiwen Pan, Yu Han, Nuo Yang, Xin Wang, Lixia Fan, Qian Li, Feifei Yin, Peng Wang, Weixin Zeng, Ting Zheng, Jifeng Zeng, Guiying Guo, Jiping Zheng
Summary: The study described the histopathological and etiological aspects of Edwardsiella tarda infection in farmed hatchling Siamese crocodiles in Hainan, China. Isolates of E. tarda from various internal organs showed positivity for virulence and antibiotic resistance genes, notably tetracycline resistance genes. Biofilm formation was highlighted as a key virulent factor in the pathogenesis of E. tarda.
