期刊
VACCINE
卷 35, 期 31, 页码 3813-3816出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2017.05.066
关键词
Edwardsiella tarda; Reverse vaccinology; Glycolysis-related protein; Protective antigen; Broad spectrum vaccine
资金
- National Natural Science Funds of China [31372550]
- Project of Marine Public Welfare Profession of China [201405003-2]
- Modern Agro-industry Technology Research System [CARS-50-G07]
- Fundamental Research Funds for the Central Universities
Reverse vaccinology (RV) has become a popular method for developing vaccines. Although Edwardsiella tarda is deemed to be an important fish pathogen, so far, no reports have used a genome-based approach to screen vaccine candidates against E. tarda. In the current study, protective antigens of E. tarda were screened using RV. Large-scale cloning, expression and purification of potential candidates were carried out, and their immunoprotective potential was evaluated. A candidate fructose-bisphosphate aldolase (FBA) exhibited broad spectrum protection, as did another glycolysis-related protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which we reported previously, indicating the potential of other glycolysis-related proteins of E. tarda as broad spectrum protective antigens. In total, half (5 out 10) of these proteins showed prominent immunoprotective potential. Therefore, we suggest that glycolysis-related proteins are a class of potential broad spectrum protective antigens and that these proteins should be preferentially selected. (C) 2017 Elsevier Ltd. All rights reserved.
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