Article
Agronomy
Heeyoung Park, Sang Un Park, Byeong-Kwan Jang, Jeong Jae Lee, Yong Suk Chung
Summary: Kenaf, an annual fiber crop mainly grown in India and China, is emerging as a promising bio-based energy source due to its rapid growth, CO2 absorption capabilities, and high productivity. The study evaluated 10 different cultivars of kenaf for cellulosic ethanol production, finding that ethanol yield and productivity were significantly impacted by cultivar type, rather than biomass composition. Engineering yeast to utilize xylose under pH control can maximize ethanol production from kenaf.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2021)
Article
Agricultural Engineering
Fengguang Zhao, Yaping Zhang, Jian Hu, Ce Shi, Xiang Ao, Shengding Wang, Ying Lin, Zhongwei Sun, Shuangyan Han
Summary: A haploid gene-deletion library was screened to identify 22 vanillin-tolerant mutants and investigate the tolerance mechanisms. The deleted genes in these mutants were found to be involved in phosphate and inositol polyphosphate metabolism and intracellular sterol transport. Activation of the phosphate signaling pathway was unfavorable for yeast under vanillin pressure, while inositol polyphosphates played a role in altering vanillin tolerance by regulating phosphate metabolism. Additionally, reducing sterol transport from the plasma membrane enhanced tolerance to vanillin. Two representative yeast deletions, pho84 Delta and lam3 Delta, showed good growth performance and rapid ethanol production in the presence of vanillin.
BIORESOURCE TECHNOLOGY
(2023)
Article
Food Science & Technology
Peizhou Yang, Jianchao Chen, Wenjing Wu, Suwei Jiang, Yanhong Deng, Jiuling Lu, Hu Wang, Yong Zhou, Yuyou Geng, Zhi Zheng
Summary: CRISPR-Cas9 was used to knock out MET5 and SIZ1 genes in S. cerevisiae MET5 and SIZ1 using XYL1 and XYL2 expression cassettes, leading to improved tolerance to organic acids and increased xylose utilization efficiency. The co-deletion of MET5 and SIZ1 resulted in enhanced tolerance to acetic acid and lactic acid. Transcriptomics analysis showed up-regulation of carbon metabolism and down-regulation of ribosome biogenesis in S. cerevisiae MET5dSIZ1d. This mutant strain exhibited higher ethanol concentrations in both kiwifruit and corn stalk hydrolysate fermentations, indicating its potential for ethanol production from cellulosic hydrolysates and substrates rich in organic acids.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Review
Acoustics
Yao Yang, Wenbin Ren, Haining Xu, Liang Cheng, Malcom Frimpong Dapaah, Ronghai He, Haile Ma
Summary: This study demonstrated an enhancement of ethanol yield in Saccharomyces cerevisiae through low-intensity ultrasonic irradiation, which involved regulations on gene expression and metabolite content related to ethanol synthesis. The findings suggest that ultrasonic treatment can effectively promote ethanol production by altering gene expression and metabolite levels in yeast cells.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Green & Sustainable Science & Technology
Phuong Hoang Nguyen Tran, Je Hyeong Jung, Ja Kyong Ko, Gyeongtaek Gong, Youngsoon Um, Sun-Mi Lee
Summary: The development of glucose/xylose co-fermenting Saccharomyces cerevisiae strain capable of co-producing polyhydroxybutyrate has improved the bioethanol yield from lignocellulosic biomass. This study demonstrates the feasibility of co-production of bioethanol and value-added chemicals to maximize the values derived from lignocellulosic biomass, using various types of biomasses.
Article
Agriculture, Multidisciplinary
Yunliang Zhang, Weigao Wang, Wenqian Wei, Lu Xia, Song Gao, Weizhu Zeng, Song Liu, Jingwen Zhou
Summary: By enhancing the expression of TSP1 and HSP104 genes in Saccharomyces cerevisiae, the ethanol tolerance of the yeast was increased, leading to higher accumulation of squalene. By optimizing the fermentation conditions, the highest reported squalene production parameters for a microorganism were achieved.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Mahmod Sidati Ali Abobaker, Husnul Azan Tajarudin, Abdul Latif Ahmad, Wan Maznah Wan Omar, Charles Ng Wai Chun
Summary: This study investigates the treatment of municipal landfill leachate using Scenedesmus sp. through batch and continuous cultivation modes. The research finds that continuous cultivation mode shows the highest removal values for COD, phosphorus, and carbohydrate. Additionally, the study suggests that Scenedesmus sp. has the potential to be utilized for bioethanol production, supporting the concept of sustainable materials.
Article
Engineering, Environmental
Minakshi Gohain, Maskura Hasin, Khalifa S. H. Eldiehy, Pritam Bardhan, Khairujjaman Laskar, Hridoyjit Phukon, Manabendra Mandal, Dipul Kalita, Dhanapati Deka
Summary: This study investigated the potential of using deoiled Scenedesmus obliquus for bio-ethanol production with different heterogeneous catalysts. Successful hydrolysis of the biomass was achieved using eco-friendly catalysts, leading to the production of bio-ethanol with a high yield and concentration through fermentation.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2021)
Article
Biotechnology & Applied Microbiology
Xianlin He, Bo Liu, Yali Xu, Ze Chen, Hao Li
Summary: The presence of Lactobacillus plantarum can increase the ethanol tolerance of Saccharomyces cerevisiae by promoting or inhibiting various metabolic processes in the yeast cells, and the maintenance of this tolerance depends on the coexistence of both organisms. The study also suggested a complex relationship between yeast and contaminating LAB, which may play a beneficial role in fermentation by promoting the ethanol tolerance of yeast.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Juhi Sharma, Vinod Kumar, Rajendra Prasad, Naseem A. Gaur
Summary: Lignocellulosic biomass is an abundant material on earth and can be processed by microorganisms to produce biofuels. Saccharomyces cerevisiae is an ideal candidate for consolidated bio-processing (CBP) to produce cellulose-based ethanol. However, there are still challenges in the process, such as incomplete substrate catabolism and low expression of heterologous proteins.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Engineering, Chemical
Wei-Yang Wang, Shi-Hua Wu, Yuan-Han Xie, Miao Zhong, Man-Li Wei, Ze-Yang Li, Xiu-Feng Long, Fu-Xing Niu
Summary: This study successfully established a Saccharomyces cerevisiae mutant library capable of tolerating high sucrose concentration, leading to increased ethanol yield and improved sucrose utilization efficiency.
Article
Agricultural Engineering
Pei-Liang Ye, Xue-Qing Wang, Bing Yuan, Chen-Guang Liu, Xin-Qing Zhao
Summary: In this study, integrated analysis of transcriptome, proteome, and phosphoproteome revealed significant changes in protein kinases in yeast cells under acetic acid stress. Overexpression of the mitogen-activated protein kinase Hog1 improved ethanol production under stress conditions.
BIORESOURCE TECHNOLOGY
(2022)
Article
Energy & Fuels
Lucia Coimbra, Karen Malan, Alejandra Fagundez, Mairan Guigou, Claudia Lareo, Belen Fernandez, Martin Pratto, Silvia Batista
Summary: Genetically engineered yeast strains were developed for efficient ethanol production from lignocellulosic biomass. The recombinant strain CAT-1-XIT (pRS42K::XI) showed promising results with 74% consumption of D-xylose and production of 12.6 g/L ethanol.
BIOENERGY RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Xueqin Lv, Ke Jin, Yu Yi, Lingang Song, Xiang Xiu, Yanfeng Liu, Jianghua Li, Guocheng Du, Jian Chen, Long Liu
Summary: By visual observation and order analysis of membrane microdomains of Saccharomyces cerevisiae, it was found that membrane microdomains play a crucial role in acid tolerance. This study proposed a new acid tolerance mechanism, providing a new direction for the rational engineering of acid-tolerant strains.
MICROBIAL CELL FACTORIES
(2023)
Article
Acoustics
Chunhua Dai, Zhenzhen Shu, Xueting Xu, Pengfei Yan, Mokhtar Dabbour, Benjamin Kumah Mintah, Liurong Huang, Ronghai He, Haile Ma
Summary: This study investigated the effect of low-intensity fixed-frequency continuous ultrasound on the growth of Bacillus licheniformis. The results showed that the ultrasound treatment significantly increased biomass, membrane permeability, and metabolic activity of the organism.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Engineering, Chemical
Ling Sun, Liping Liu, Lei Yang, Yazhen Wang, Mokhtar Dabbour, Benjamin Kumah Mintah, Ronghai He, Haile Ma
Summary: The use of ultrasound technology has shown to enhance the growth and metabolism of Ganoderma lucidum, leading to increased biomass and metabolite production. This study provides a theoretical basis for the application of ultrasound in the microbial fermentation industry.
JOURNAL OF FOOD PROCESS ENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
Ekene Christopher Umego, Ronghai He, Wenbin Ren, Haining Xu, Haile Ma
Summary: Ultrasonic-assisted enzymolysis, utilizing ultrasound technology to improve enzymolysis process, has shown potential in enhancing nutrient bioavailability and accelerating enzyme hydrolysis.
PROCESS BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Qingzhi Ding, Arooj Rehman Sheikh, Wenwen Pan, Xiangyue Gu, Nianzhen Sun, Xiaodong Su, Lin Luo, Haile Ma, Ronghai He, Ting Zhang
Summary: Raman spectroscopy was used to monitor the enzymatic hydrolysis process of grape seed protein, and models for DH, IC50, and peptide content were established and optimized. The results showed the feasibility of real-time monitoring of grape seed protein hydrolysis using Raman spectroscopy, which is significant for the grape seed active peptide industry.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Article
Chemistry, Applied
Yang Wang, Zhaoli Zhang, Ronghai He, Benjamin Kumah Mintah, Mokhtar Dabbour, Wenjuan Qu, Dandan Liu, Haile Ma
Summary: The use of sequential and simultaneous duple-frequency modes of ultrasound enhanced the enzymolysis efficiency of corn gluten meal (CGM) significantly, with the 20/40 kHz sequential duple-frequency mode showing the most significant effect. Changes in surface hydrophobicity, secondary structure, and microstructure of CGM were observed, along with an increase in susceptibility to alcalase proteolysis. Ultrasonication may be a practical approach to improve protein-enzyme reactions.
Review
Food Science & Technology
Qingzhi Ding, Arooj Rehman Sheikh, Qian Chen, Yize Hu, Nianzhen Sun, Xiaodong Su, Lin Luo, Haile Ma, Ronghai He
Summary: In this study, the structural changes and influencing factors of ACE inhibitory peptides were analyzed through comparative analysis of polypeptide amino acid sequences. The results showed that leucine is the N-terminal peptide responsible for ACE inhibition and proline is the C-terminal peptide, and lower molecular weight peptides showed higher ACEI.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Agriculture, Multidisciplinary
Chunhua Dai, Yizhi Hou, Haining Xu, Liurong Huang, Mokhtar Dabbour, Benjamin K. Mintah, Ronghai He, Haile Ma
Summary: The study found that fermentation by thermophiles such as Bacillus licheniformis YYC4 and mesophilic Bacillus subtilis 10 160 could significantly improve the protein content and structure of soybean meal, indicating potential application in the SBM fermentation industry.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Agriculture, Multidisciplinary
Haining Xu, Chunhua Dai, Yingxiu Tang, Xueting Xu, Ekene Christopher Umego, Ronghai He, Haile Ma
Summary: The study utilized ARTP mutagenesis technology to successfully increase the production of Surfactin in Bacillus subtilis, resulting in the mutant strain M45 with higher growth rate and yield. The findings provide a basis for industrial production of Surfactin and shed light on the mutagenesis mechanism.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Agriculture, Multidisciplinary
Chunhua Dai, Yizhi Hou, Haining Xu, Ekene C. Umego, Liurong Huang, Ronghai He, Haile Ma
Summary: Thermophiles like Bacillus licheniformis can produce enzymes with biocatalytic potential. By utilizing strain YYC4 in solid-state fermentation, significant increases in soluble and crude protein content, along with a decrease in harmful trypsin inhibitor activity, were achieved. The dominant strain Bacillus licheniformis YYC4 inhibited most other microorganisms during fermentation, demonstrating the potential application of thermophiles in a eco-friendly and energy-saving manner.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Engineering, Chemical
Mokhtar Dabbour, Rokkaya Sami, Benjamin K. Mintah, Ronghai He, Hafida Wahia, Ebtihal Khojah, Anka Trajkovska Petkoska, Mohammad Fikry
Summary: The influence of freeze and convection drying on the physical, functional, and rheological attributes of sunflower protein and its hydrolysate was studied. Freeze-dried samples had higher lightness, turbidity, bulk density, and particle size values, while having lower browning index. They also showed good solubility and foaming characteristics. Convectively-dried powders exhibited greater viscosity and consistency coefficient, and lower flow behavior index of dispersions.
Review
Engineering, Chemical
Jiayin Pan, Zhaoli Zhang, Benjamin Kumah Mintah, Haining Xu, Mokhtar Dabbour, Yu Cheng, Chunhua Dai, Ronghai He, Haile Ma
Summary: This review assesses the impact of nonthermal physical processing technologies on protein modification and discusses the structural, functional, and digestibility attributes of processed proteins. Nonthermal physical technologies, such as ultrasonication, ultrahigh pressure, and pulsed electric field (PEF), can induce conformational changes in proteins and influence their functional properties. These technologies have shown significant achievements in improving enzymatic hydrolysis, solubility, emulsification, foaming, gelatinization, digestibility, and reducing allergenicity.
JOURNAL OF FOOD PROCESS ENGINEERING
(2022)
Article
Food Science & Technology
Hui Jiang, Zheng Xing, Mokhtar Dabbour, Benjamin Kumah Mintah, Zhaoli Zhang, Ronghai He, Haile Ma
Summary: Studies have shown that the conjugate formed by allicin and whey protein isolate can enhance the stability of allicin. Optimal reaction conditions were determined, and the effect of binding rate on the properties and microstructure of the conjugates was investigated.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Lina Guo, Yiting Guo, Ping Wu, Shiyi Liu, Chen Gu, Yolandani, Mengdi Wu, Haile Ma, Ronghai He
Summary: The study showed that low-intensity alternating magnetic field-assisted solid-state fermentation could effectively enhance the polypeptide yield from rapeseed meal, providing a new approach for the production of bioactive components.
Review
Food Science & Technology
Jiayin Pan, Haining Xu, Yu Cheng, Benjamin Kumah Mintah, Mokhtar Dabbour, Fan Yang, Wen Chen, Zhaoli Zhang, Chunhua Dai, Ronghai He, Haile Ma
Summary: This article explores the characteristics, extraction process, biological activity, and applications of insect protein as an alternative protein source.
Review
Food Science & Technology
Yang Wang, Jiarui Liu, Zhaoli Zhang, Xiangren Meng, Tingxuan Yang, Wangbin Shi, Ronghai He, Haile Ma
Summary: This article discusses the effects of ultrasonication on the characteristics of cereal proteins (CPs), including solubility, emulsibility, foamability, surface-hydrophobicity, particle-size, conformational-structure, microstructural, enzymatic-hydrolysis, and digestive properties. The results show that ultrasonication can enhance the characteristics of CPs, improving solubility, emulsibility, and foamability, and altering protein structures. Additionally, ultrasonication can promote enzymatic efficiency and in vitro digestibility of CPs. Therefore, ultrasonication technology is a useful method for modifying cereal protein functionality and structure for the food industry.
Article
Food Science & Technology
Zhaoli Zhang, Tingxuan Yang, Yang Wang, Jiarui Liu, Wangbin Shi, Haochen Hu, Yang Meng, Xiangren Meng, Ronghai He
Summary: This study investigated the impact of multi-frequency sonication on the structural characteristics of beef myofibrillar proteins (MPs) at different degrees of doneness. The results revealed that the surface hydrophobicity and sulfhydryl content of the MPs increased with the degree of doneness. The sulfhydryl group content was maximized at a frequency of 28 kHz. Furthermore, ultrasonic treatment significantly decreased the -potential value, and the structural changes in the MPs were observed through various techniques such as SDS gel electrophoresis, Fourier transform infrared spectrum, and atomic force microscopy.
